Frankia and Alnus rubra canopy roots: an assessment of genetic diversity, propagule availability, and effects on soil nitrogen.
Identifieur interne : 000246 ( Main/Exploration ); précédent : 000245; suivant : 000247Frankia and Alnus rubra canopy roots: an assessment of genetic diversity, propagule availability, and effects on soil nitrogen.
Auteurs : Peter G. Kennedy [États-Unis] ; Jesse L. Schouboe ; Rachel H. Rogers ; Marjorie G. Weber ; Nalini M. NadkarniSource :
- Microbial ecology [ 1432-184X ] ; 2010.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), Alnus (microbiologie), Arbres (génétique), Azote (analyse), Frankia (classification), Frankia (génétique), Génotype (MeSH), Microbiologie du sol (MeSH), Phylogenèse (MeSH), Racines de plante (microbiologie), Sol (analyse), Symbiose (MeSH), Variation génétique (MeSH), Washington (MeSH).
- MESH :
- analyse : Azote, Sol.
- génétique : ADN bactérien, Arbres, Frankia.
- microbiologie : Alnus, Racines de plante.
- Génotype, Microbiologie du sol, Phylogenèse, Symbiose, Variation génétique, Washington.
English descriptors
- KwdEn :
- Alnus (microbiology), DNA, Bacterial (genetics), Frankia (classification), Frankia (genetics), Genetic Variation (MeSH), Genotype (MeSH), Nitrogen (analysis), Phylogeny (MeSH), Plant Roots (microbiology), Soil (analysis), Soil Microbiology (MeSH), Symbiosis (MeSH), Trees (genetics), Washington (MeSH).
- MESH :
- chemical , analysis : Nitrogen, Soil.
- chemical , genetics : DNA, Bacterial.
- classification : Frankia.
- genetics : Frankia, Trees.
- microbiology : Alnus, Plant Roots.
- Genetic Variation, Genotype, Phylogeny, Soil Microbiology, Symbiosis, Washington.
Abstract
The ecological importance of microbial symbioses in terrestrial soils is widely recognized, but their role in soils that accumulate in forest canopies is almost entirely unknown. To address this gap, this study investigated the Frankia-Alnus rubra symbiosis in canopy and forest floor roots at Olympic National Park, WA, USA. Sixteen mature A. rubra trees were surveyed and Frankia genetic diversity in canopy and forest floor nodules was assessed with sequence-based nifH analyses. A seedling bioassay experiment was conducted to determine Frankia propagule availability in canopy and forest floor soils. Total soil nitrogen from both environments was also quantified. Nodules were present in the canopies of nine of the 16 trees sampled. Across the study area, Frankia canopy and forest floor assemblages were similar, with both habitats containing the same two genotypes. The composition of forest floor and canopy genotypes on the same tree was not always identical, however, suggesting that dispersal was not a strictly local phenomenon. Frankia seedling colonization was similar in canopy soils regardless of the presence of nodules as well as in forest floor soils, indicating that dispersal was not likely to be a major limiting factor. The total soil nitrogen of canopy soils was higher than that of forest floor soils, but the presence of Frankia nodules in canopy soils did not significantly alter soil nitrogen levels. Overall, this study indicates that the Frankia-A. rubra symbiosis is similar in canopy and forest floor environments. Because canopy roots are exposed to different environmental conditions within very small spatial areas and because those areas can be easily manipulated (e.g., fertilizer or watering treatments), they present microbial ecologists with a unique arena to examine root-microbe interactions.
DOI: 10.1007/s00248-009-9587-8
PubMed: 19787390
Affiliations:
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Schouboe</name></author><author><name sortKey="Rogers, Rachel H" sort="Rogers, Rachel H" uniqKey="Rogers R" first="Rachel H" last="Rogers">Rachel H. Rogers</name></author><author><name sortKey="Weber, Marjorie G" sort="Weber, Marjorie G" uniqKey="Weber M" first="Marjorie G" last="Weber">Marjorie G. Weber</name></author><author><name sortKey="Nadkarni, Nalini M" sort="Nadkarni, Nalini M" uniqKey="Nadkarni N" first="Nalini M" last="Nadkarni">Nalini M. 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Kennedy</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biology, Lewis and Clark College, Portland, OR 97219, USA. pkennedy@lclark.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, Lewis and Clark College, Portland, OR 97219</wicri:regionArea><placeName><region type="state">Oregon</region></placeName></affiliation></author><author><name sortKey="Schouboe, Jesse L" sort="Schouboe, Jesse L" uniqKey="Schouboe J" first="Jesse L" last="Schouboe">Jesse L. Schouboe</name></author><author><name sortKey="Rogers, Rachel H" sort="Rogers, Rachel H" uniqKey="Rogers R" first="Rachel H" last="Rogers">Rachel H. Rogers</name></author><author><name sortKey="Weber, Marjorie G" sort="Weber, Marjorie G" uniqKey="Weber M" first="Marjorie G" last="Weber">Marjorie G. Weber</name></author><author><name sortKey="Nadkarni, Nalini M" sort="Nadkarni, Nalini M" uniqKey="Nadkarni N" first="Nalini M" last="Nadkarni">Nalini M. Nadkarni</name></author></analytic><series><title level="j">Microbial ecology</title><idno type="eISSN">1432-184X</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alnus (microbiology)</term><term>DNA, Bacterial (genetics)</term><term>Frankia (classification)</term><term>Frankia (genetics)</term><term>Genetic Variation (MeSH)</term><term>Genotype (MeSH)</term><term>Nitrogen (analysis)</term><term>Phylogeny (MeSH)</term><term>Plant Roots (microbiology)</term><term>Soil (analysis)</term><term>Soil Microbiology (MeSH)</term><term>Symbiosis (MeSH)</term><term>Trees (genetics)</term><term>Washington (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (génétique)</term><term>Alnus (microbiologie)</term><term>Arbres (génétique)</term><term>Azote (analyse)</term><term>Frankia (classification)</term><term>Frankia (génétique)</term><term>Génotype (MeSH)</term><term>Microbiologie du sol (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Racines de plante (microbiologie)</term><term>Sol (analyse)</term><term>Symbiose (MeSH)</term><term>Variation génétique (MeSH)</term><term>Washington (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Nitrogen</term><term>Soil</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Azote</term><term>Sol</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Frankia</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Frankia</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>Arbres</term><term>Frankia</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Alnus</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Alnus</term><term>Plant Roots</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genetic Variation</term><term>Genotype</term><term>Phylogeny</term><term>Soil Microbiology</term><term>Symbiosis</term><term>Washington</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Génotype</term><term>Microbiologie du sol</term><term>Phylogenèse</term><term>Symbiose</term><term>Variation génétique</term><term>Washington</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The ecological importance of microbial symbioses in terrestrial soils is widely recognized, but their role in soils that accumulate in forest canopies is almost entirely unknown. To address this gap, this study investigated the Frankia-Alnus rubra symbiosis in canopy and forest floor roots at Olympic National Park, WA, USA. Sixteen mature A. rubra trees were surveyed and Frankia genetic diversity in canopy and forest floor nodules was assessed with sequence-based nifH analyses. A seedling bioassay experiment was conducted to determine Frankia propagule availability in canopy and forest floor soils. Total soil nitrogen from both environments was also quantified. Nodules were present in the canopies of nine of the 16 trees sampled. Across the study area, Frankia canopy and forest floor assemblages were similar, with both habitats containing the same two genotypes. The composition of forest floor and canopy genotypes on the same tree was not always identical, however, suggesting that dispersal was not a strictly local phenomenon. Frankia seedling colonization was similar in canopy soils regardless of the presence of nodules as well as in forest floor soils, indicating that dispersal was not likely to be a major limiting factor. The total soil nitrogen of canopy soils was higher than that of forest floor soils, but the presence of Frankia nodules in canopy soils did not significantly alter soil nitrogen levels. Overall, this study indicates that the Frankia-A. rubra symbiosis is similar in canopy and forest floor environments. Because canopy roots are exposed to different environmental conditions within very small spatial areas and because those areas can be easily manipulated (e.g., fertilizer or watering treatments), they present microbial ecologists with a unique arena to examine root-microbe interactions.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19787390</PMID><DateCompleted><Year>2010</Year><Month>04</Month><Day>12</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-184X</ISSN><JournalIssue CitedMedium="Internet"><Volume>59</Volume><Issue>2</Issue><PubDate><Year>2010</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Microbial ecology</Title><ISOAbbreviation>Microb Ecol</ISOAbbreviation></Journal><ArticleTitle>Frankia and Alnus rubra canopy roots: an assessment of genetic diversity, propagule availability, and effects on soil nitrogen.</ArticleTitle><Pagination><MedlinePgn>214-20</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00248-009-9587-8</ELocationID><Abstract><AbstractText>The ecological importance of microbial symbioses in terrestrial soils is widely recognized, but their role in soils that accumulate in forest canopies is almost entirely unknown. To address this gap, this study investigated the Frankia-Alnus rubra symbiosis in canopy and forest floor roots at Olympic National Park, WA, USA. Sixteen mature A. rubra trees were surveyed and Frankia genetic diversity in canopy and forest floor nodules was assessed with sequence-based nifH analyses. A seedling bioassay experiment was conducted to determine Frankia propagule availability in canopy and forest floor soils. Total soil nitrogen from both environments was also quantified. Nodules were present in the canopies of nine of the 16 trees sampled. Across the study area, Frankia canopy and forest floor assemblages were similar, with both habitats containing the same two genotypes. The composition of forest floor and canopy genotypes on the same tree was not always identical, however, suggesting that dispersal was not a strictly local phenomenon. Frankia seedling colonization was similar in canopy soils regardless of the presence of nodules as well as in forest floor soils, indicating that dispersal was not likely to be a major limiting factor. The total soil nitrogen of canopy soils was higher than that of forest floor soils, but the presence of Frankia nodules in canopy soils did not significantly alter soil nitrogen levels. Overall, this study indicates that the Frankia-A. rubra symbiosis is similar in canopy and forest floor environments. Because canopy roots are exposed to different environmental conditions within very small spatial areas and because those areas can be easily manipulated (e.g., fertilizer or watering treatments), they present microbial ecologists with a unique arena to examine root-microbe interactions.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kennedy</LastName><ForeName>Peter G</ForeName><Initials>PG</Initials><AffiliationInfo><Affiliation>Department of Biology, Lewis and Clark College, Portland, OR 97219, USA. pkennedy@lclark.edu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schouboe</LastName><ForeName>Jesse L</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Rogers</LastName><ForeName>Rachel H</ForeName><Initials>RH</Initials></Author><Author ValidYN="Y"><LastName>Weber</LastName><ForeName>Marjorie G</ForeName><Initials>MG</Initials></Author><Author ValidYN="Y"><LastName>Nadkarni</LastName><ForeName>Nalini M</ForeName><Initials>NM</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>09</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Microb Ecol</MedlineTA><NlmUniqueID>7500663</NlmUniqueID><ISSNLinking>0095-3628</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012987">Soil</NameOfSubstance></Chemical><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance 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MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012987" MajorTopicYN="N">Soil</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="N">Soil Microbiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="Y">Symbiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014861" MajorTopicYN="N">Washington</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>07</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>08</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2009</Year><Month>08</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>9</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>9</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>4</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19787390</ArticleId><ArticleId 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IdType="pubmed">8336669</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Oregon</li></region></list><tree><noCountry><name sortKey="Nadkarni, Nalini M" sort="Nadkarni, Nalini M" uniqKey="Nadkarni N" first="Nalini M" last="Nadkarni">Nalini M. Nadkarni</name><name sortKey="Rogers, Rachel H" sort="Rogers, Rachel H" uniqKey="Rogers R" first="Rachel H" last="Rogers">Rachel H. Rogers</name><name sortKey="Schouboe, Jesse L" sort="Schouboe, Jesse L" uniqKey="Schouboe J" first="Jesse L" last="Schouboe">Jesse L. Schouboe</name><name sortKey="Weber, Marjorie G" sort="Weber, Marjorie G" uniqKey="Weber M" first="Marjorie G" last="Weber">Marjorie G. Weber</name></noCountry><country name="États-Unis"><region name="Oregon"><name sortKey="Kennedy, Peter G" sort="Kennedy, Peter G" uniqKey="Kennedy P" first="Peter G" last="Kennedy">Peter G. Kennedy</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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