Serveur d'exploration sur les interactions arbre microorganisme

Attention, ce site est en cours de développement !
Attention, site généré par des moyens informatiques à partir de corpus bruts.
Les informations ne sont donc pas validées.

Metal induction of a Paxillus involutus metallothionein and its heterologous expression in Hebeloma cylindrosporum.

Identifieur interne : 000266 ( Main/Corpus ); précédent : 000265; suivant : 000267

Metal induction of a Paxillus involutus metallothionein and its heterologous expression in Hebeloma cylindrosporum.

Auteurs : Marc Bellion ; Mikael Courbot ; Christophe Jacob ; Frédéric Guinet ; Damien Blaudez ; Michel Chalot

Source :

RBID : pubmed:17335505

English descriptors

Abstract

* Metallothioneins are small polypeptides involved in metal tolerance of many eukaryotes. Here we characterized the Pimt1 gene, coding for a metallothionein from the ectomycorrhizal fungus Paxillus involutus. * Expression of Pimt1 in P. involutus under metal stress conditions was measured by northern blot and RT-PCR analyses. The full-length cDNA was used to perform functional complementation in yeast mutant strains and agrotransformation of Hebeloma cylindrosporum. * Heterologous expression in yeast showed that PiMT1 was able to complement the hypersensitivity of mutant strains to cadmium (Cd) and copper (Cu), but not to zinc (Zn). Transcripts were almost undetectable under control conditions, whereas Cu and Cd, but not Zn, strongly induced Pimt1 expression in P. involutus. Constitutive overexpression of Pimt1 in H. cylindrosporum conferred a higher copper tolerance. * The present study identified PiMT1 as a potential determinant in the response of mycorrhizal fungi to Cu and Cd stress. Additionally, we demonstrated the usefulness of mycorrhizal fungi transformation using Agrobacterium technology to approach gene function.

DOI: 10.1111/j.1469-8137.2007.01973.x
PubMed: 17335505

Links to Exploration step

pubmed:17335505

Le document en format XML

<record>
<TEI>
<teiHeader>
<fileDesc>
<titleStmt>
<title xml:lang="en">Metal induction of a Paxillus involutus metallothionein and its heterologous expression in Hebeloma cylindrosporum.</title>
<author>
<name sortKey="Bellion, Marc" sort="Bellion, Marc" uniqKey="Bellion M" first="Marc" last="Bellion">Marc Bellion</name>
<affiliation>
<nlm:affiliation>Nancy-University, Research Unit 1136 INRA/UHP Tree-Microbe Interactions, BP 239, F-54506 Vandoeuvre-les-Nancy Cedex, France.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Courbot, Mikael" sort="Courbot, Mikael" uniqKey="Courbot M" first="Mikael" last="Courbot">Mikael Courbot</name>
</author>
<author>
<name sortKey="Jacob, Christophe" sort="Jacob, Christophe" uniqKey="Jacob C" first="Christophe" last="Jacob">Christophe Jacob</name>
</author>
<author>
<name sortKey="Guinet, Frederic" sort="Guinet, Frederic" uniqKey="Guinet F" first="Frédéric" last="Guinet">Frédéric Guinet</name>
</author>
<author>
<name sortKey="Blaudez, Damien" sort="Blaudez, Damien" uniqKey="Blaudez D" first="Damien" last="Blaudez">Damien Blaudez</name>
</author>
<author>
<name sortKey="Chalot, Michel" sort="Chalot, Michel" uniqKey="Chalot M" first="Michel" last="Chalot">Michel Chalot</name>
</author>
</titleStmt>
<publicationStmt>
<idno type="wicri:source">PubMed</idno>
<date when="2007">2007</date>
<idno type="RBID">pubmed:17335505</idno>
<idno type="pmid">17335505</idno>
<idno type="doi">10.1111/j.1469-8137.2007.01973.x</idno>
<idno type="wicri:Area/Main/Corpus">000266</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000266</idno>
</publicationStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title xml:lang="en">Metal induction of a Paxillus involutus metallothionein and its heterologous expression in Hebeloma cylindrosporum.</title>
<author>
<name sortKey="Bellion, Marc" sort="Bellion, Marc" uniqKey="Bellion M" first="Marc" last="Bellion">Marc Bellion</name>
<affiliation>
<nlm:affiliation>Nancy-University, Research Unit 1136 INRA/UHP Tree-Microbe Interactions, BP 239, F-54506 Vandoeuvre-les-Nancy Cedex, France.</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Courbot, Mikael" sort="Courbot, Mikael" uniqKey="Courbot M" first="Mikael" last="Courbot">Mikael Courbot</name>
</author>
<author>
<name sortKey="Jacob, Christophe" sort="Jacob, Christophe" uniqKey="Jacob C" first="Christophe" last="Jacob">Christophe Jacob</name>
</author>
<author>
<name sortKey="Guinet, Frederic" sort="Guinet, Frederic" uniqKey="Guinet F" first="Frédéric" last="Guinet">Frédéric Guinet</name>
</author>
<author>
<name sortKey="Blaudez, Damien" sort="Blaudez, Damien" uniqKey="Blaudez D" first="Damien" last="Blaudez">Damien Blaudez</name>
</author>
<author>
<name sortKey="Chalot, Michel" sort="Chalot, Michel" uniqKey="Chalot M" first="Michel" last="Chalot">Michel Chalot</name>
</author>
</analytic>
<series>
<title level="j">The New phytologist</title>
<idno type="ISSN">0028-646X</idno>
<imprint>
<date when="2007" type="published">2007</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc>
<textClass>
<keywords scheme="KwdEn" xml:lang="en">
<term>Agaricales (genetics)</term>
<term>Agaricales (metabolism)</term>
<term>Amino Acid Sequence (MeSH)</term>
<term>Basidiomycota (genetics)</term>
<term>Basidiomycota (metabolism)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>DNA, Complementary (MeSH)</term>
<term>Fungal Proteins (biosynthesis)</term>
<term>Fungal Proteins (chemistry)</term>
<term>Fungal Proteins (genetics)</term>
<term>Gene Expression Regulation, Fungal (MeSH)</term>
<term>Genetic Complementation Test (MeSH)</term>
<term>Metallothionein (biosynthesis)</term>
<term>Metallothionein (chemistry)</term>
<term>Metallothionein (genetics)</term>
<term>Metals (metabolism)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Mycorrhizae (genetics)</term>
<term>Mycorrhizae (metabolism)</term>
<term>Organisms, Genetically Modified (MeSH)</term>
<term>Rhizobium (genetics)</term>
<term>Saccharomyces cerevisiae (genetics)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Sequence Analysis, Protein (MeSH)</term>
<term>Transcription, Genetic (MeSH)</term>
<term>Transformation, Genetic (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en">
<term>Fungal Proteins</term>
<term>Metallothionein</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en">
<term>Fungal Proteins</term>
<term>Metallothionein</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en">
<term>Fungal Proteins</term>
<term>Metallothionein</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en">
<term>Metals</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en">
<term>DNA, Complementary</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en">
<term>Agaricales</term>
<term>Basidiomycota</term>
<term>Mycorrhizae</term>
<term>Rhizobium</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en">
<term>Agaricales</term>
<term>Basidiomycota</term>
<term>Mycorrhizae</term>
</keywords>
<keywords scheme="MESH" xml:lang="en">
<term>Amino Acid Sequence</term>
<term>Cloning, Molecular</term>
<term>Gene Expression Regulation, Fungal</term>
<term>Genetic Complementation Test</term>
<term>Molecular Sequence Data</term>
<term>Organisms, Genetically Modified</term>
<term>Sequence Alignment</term>
<term>Sequence Analysis, Protein</term>
<term>Transcription, Genetic</term>
<term>Transformation, Genetic</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">* Metallothioneins are small polypeptides involved in metal tolerance of many eukaryotes. Here we characterized the Pimt1 gene, coding for a metallothionein from the ectomycorrhizal fungus Paxillus involutus. * Expression of Pimt1 in P. involutus under metal stress conditions was measured by northern blot and RT-PCR analyses. The full-length cDNA was used to perform functional complementation in yeast mutant strains and agrotransformation of Hebeloma cylindrosporum. * Heterologous expression in yeast showed that PiMT1 was able to complement the hypersensitivity of mutant strains to cadmium (Cd) and copper (Cu), but not to zinc (Zn). Transcripts were almost undetectable under control conditions, whereas Cu and Cd, but not Zn, strongly induced Pimt1 expression in P. involutus. Constitutive overexpression of Pimt1 in H. cylindrosporum conferred a higher copper tolerance. * The present study identified PiMT1 as a potential determinant in the response of mycorrhizal fungi to Cu and Cd stress. Additionally, we demonstrated the usefulness of mycorrhizal fungi transformation using Agrobacterium technology to approach gene function.</div>
</front>
</TEI>
<pubmed>
<MedlineCitation Status="MEDLINE" Owner="NLM">
<PMID Version="1">17335505</PMID>
<DateCompleted>
<Year>2007</Year>
<Month>06</Month>
<Day>08</Day>
</DateCompleted>
<DateRevised>
<Year>2020</Year>
<Month>09</Month>
<Day>30</Day>
</DateRevised>
<Article PubModel="Print">
<Journal>
<ISSN IssnType="Print">0028-646X</ISSN>
<JournalIssue CitedMedium="Print">
<Volume>174</Volume>
<Issue>1</Issue>
<PubDate>
<Year>2007</Year>
</PubDate>
</JournalIssue>
<Title>The New phytologist</Title>
<ISOAbbreviation>New Phytol</ISOAbbreviation>
</Journal>
<ArticleTitle>Metal induction of a Paxillus involutus metallothionein and its heterologous expression in Hebeloma cylindrosporum.</ArticleTitle>
<Pagination>
<MedlinePgn>151-8</MedlinePgn>
</Pagination>
<Abstract>
<AbstractText>* Metallothioneins are small polypeptides involved in metal tolerance of many eukaryotes. Here we characterized the Pimt1 gene, coding for a metallothionein from the ectomycorrhizal fungus Paxillus involutus. * Expression of Pimt1 in P. involutus under metal stress conditions was measured by northern blot and RT-PCR analyses. The full-length cDNA was used to perform functional complementation in yeast mutant strains and agrotransformation of Hebeloma cylindrosporum. * Heterologous expression in yeast showed that PiMT1 was able to complement the hypersensitivity of mutant strains to cadmium (Cd) and copper (Cu), but not to zinc (Zn). Transcripts were almost undetectable under control conditions, whereas Cu and Cd, but not Zn, strongly induced Pimt1 expression in P. involutus. Constitutive overexpression of Pimt1 in H. cylindrosporum conferred a higher copper tolerance. * The present study identified PiMT1 as a potential determinant in the response of mycorrhizal fungi to Cu and Cd stress. Additionally, we demonstrated the usefulness of mycorrhizal fungi transformation using Agrobacterium technology to approach gene function.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y">
<Author ValidYN="Y">
<LastName>Bellion</LastName>
<ForeName>Marc</ForeName>
<Initials>M</Initials>
<AffiliationInfo>
<Affiliation>Nancy-University, Research Unit 1136 INRA/UHP Tree-Microbe Interactions, BP 239, F-54506 Vandoeuvre-les-Nancy Cedex, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Courbot</LastName>
<ForeName>Mikael</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Jacob</LastName>
<ForeName>Christophe</ForeName>
<Initials>C</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Guinet</LastName>
<ForeName>Frédéric</ForeName>
<Initials>F</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Blaudez</LastName>
<ForeName>Damien</ForeName>
<Initials>D</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Chalot</LastName>
<ForeName>Michel</ForeName>
<Initials>M</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList>
<PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo>
<Country>England</Country>
<MedlineTA>New Phytol</MedlineTA>
<NlmUniqueID>9882884</NlmUniqueID>
<ISSNLinking>0028-646X</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance>
</Chemical>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance>
</Chemical>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D008670">Metals</NameOfSubstance>
</Chemical>
<Chemical>
<RegistryNumber>9038-94-2</RegistryNumber>
<NameOfSubstance UI="D008668">Metallothionein</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<CommentsCorrectionsList>
<CommentsCorrections RefType="CommentIn">
<RefSource>New Phytol. 2007;174(1):3-6</RefSource>
<PMID Version="1">17335491</PMID>
</CommentsCorrections>
</CommentsCorrectionsList>
<MeshHeadingList>
<MeshHeading>
<DescriptorName UI="D000363" MajorTopicYN="N">Agaricales</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName>
<QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D005816" MajorTopicYN="N">Genetic Complementation Test</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D008668" MajorTopicYN="N">Metallothionein</DescriptorName>
<QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D008670" MajorTopicYN="N">Metals</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D030781" MajorTopicYN="N">Organisms, Genetically Modified</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D020539" MajorTopicYN="N">Sequence Analysis, Protein</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData>
<History>
<PubMedPubDate PubStatus="pubmed">
<Year>2007</Year>
<Month>3</Month>
<Day>6</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline">
<Year>2007</Year>
<Month>6</Month>
<Day>9</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez">
<Year>2007</Year>
<Month>3</Month>
<Day>6</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList>
<ArticleId IdType="pubmed">17335505</ArticleId>
<ArticleId IdType="pii">NPH1973</ArticleId>
<ArticleId IdType="doi">10.1111/j.1469-8137.2007.01973.x</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/TreeMicInterV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000266 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000266 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    TreeMicInterV1
   |flux=    Main
   |étape=   Corpus
   |type=    RBID
   |clé=     pubmed:17335505
   |texte=   Metal induction of a Paxillus involutus metallothionein and its heterologous expression in Hebeloma cylindrosporum.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i   -Sk "pubmed:17335505" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd   \
       | NlmPubMed2Wicri -a TreeMicInterV1 

Wicri

This area was generated with Dilib version V0.6.37.
Data generation: Thu Nov 19 16:52:21 2020. Site generation: Thu Nov 19 16:52:50 2020