Metal induction of a Paxillus involutus metallothionein and its heterologous expression in Hebeloma cylindrosporum.
Identifieur interne : 000266 ( Main/Corpus ); précédent : 000265; suivant : 000267Metal induction of a Paxillus involutus metallothionein and its heterologous expression in Hebeloma cylindrosporum.
Auteurs : Marc Bellion ; Mikael Courbot ; Christophe Jacob ; Frédéric Guinet ; Damien Blaudez ; Michel ChalotSource :
- The New phytologist [ 0028-646X ] ; 2007.
English descriptors
- KwdEn :
- Agaricales (genetics), Agaricales (metabolism), Amino Acid Sequence (MeSH), Basidiomycota (genetics), Basidiomycota (metabolism), Cloning, Molecular (MeSH), DNA, Complementary (MeSH), Fungal Proteins (biosynthesis), Fungal Proteins (chemistry), Fungal Proteins (genetics), Gene Expression Regulation, Fungal (MeSH), Genetic Complementation Test (MeSH), Metallothionein (biosynthesis), Metallothionein (chemistry), Metallothionein (genetics), Metals (metabolism), Molecular Sequence Data (MeSH), Mycorrhizae (genetics), Mycorrhizae (metabolism), Organisms, Genetically Modified (MeSH), Rhizobium (genetics), Saccharomyces cerevisiae (genetics), Sequence Alignment (MeSH), Sequence Analysis, Protein (MeSH), Transcription, Genetic (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , biosynthesis : Fungal Proteins, Metallothionein.
- chemical , chemistry : Fungal Proteins, Metallothionein.
- chemical , genetics : Fungal Proteins, Metallothionein.
- chemical , metabolism : Metals.
- chemical : DNA, Complementary.
- genetics : Agaricales, Basidiomycota, Mycorrhizae, Rhizobium, Saccharomyces cerevisiae.
- metabolism : Agaricales, Basidiomycota, Mycorrhizae.
- Amino Acid Sequence, Cloning, Molecular, Gene Expression Regulation, Fungal, Genetic Complementation Test, Molecular Sequence Data, Organisms, Genetically Modified, Sequence Alignment, Sequence Analysis, Protein, Transcription, Genetic, Transformation, Genetic.
Abstract
* Metallothioneins are small polypeptides involved in metal tolerance of many eukaryotes. Here we characterized the Pimt1 gene, coding for a metallothionein from the ectomycorrhizal fungus Paxillus involutus. * Expression of Pimt1 in P. involutus under metal stress conditions was measured by northern blot and RT-PCR analyses. The full-length cDNA was used to perform functional complementation in yeast mutant strains and agrotransformation of Hebeloma cylindrosporum. * Heterologous expression in yeast showed that PiMT1 was able to complement the hypersensitivity of mutant strains to cadmium (Cd) and copper (Cu), but not to zinc (Zn). Transcripts were almost undetectable under control conditions, whereas Cu and Cd, but not Zn, strongly induced Pimt1 expression in P. involutus. Constitutive overexpression of Pimt1 in H. cylindrosporum conferred a higher copper tolerance. * The present study identified PiMT1 as a potential determinant in the response of mycorrhizal fungi to Cu and Cd stress. Additionally, we demonstrated the usefulness of mycorrhizal fungi transformation using Agrobacterium technology to approach gene function.
DOI: 10.1111/j.1469-8137.2007.01973.x
PubMed: 17335505
Links to Exploration step
pubmed:17335505Le document en format XML
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<author><name sortKey="Courbot, Mikael" sort="Courbot, Mikael" uniqKey="Courbot M" first="Mikael" last="Courbot">Mikael Courbot</name>
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<author><name sortKey="Jacob, Christophe" sort="Jacob, Christophe" uniqKey="Jacob C" first="Christophe" last="Jacob">Christophe Jacob</name>
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<author><name sortKey="Guinet, Frederic" sort="Guinet, Frederic" uniqKey="Guinet F" first="Frédéric" last="Guinet">Frédéric Guinet</name>
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<author><name sortKey="Blaudez, Damien" sort="Blaudez, Damien" uniqKey="Blaudez D" first="Damien" last="Blaudez">Damien Blaudez</name>
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<series><title level="j">The New phytologist</title>
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<term>Amino Acid Sequence (MeSH)</term>
<term>Basidiomycota (genetics)</term>
<term>Basidiomycota (metabolism)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>DNA, Complementary (MeSH)</term>
<term>Fungal Proteins (biosynthesis)</term>
<term>Fungal Proteins (chemistry)</term>
<term>Fungal Proteins (genetics)</term>
<term>Gene Expression Regulation, Fungal (MeSH)</term>
<term>Genetic Complementation Test (MeSH)</term>
<term>Metallothionein (biosynthesis)</term>
<term>Metallothionein (chemistry)</term>
<term>Metallothionein (genetics)</term>
<term>Metals (metabolism)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Mycorrhizae (genetics)</term>
<term>Mycorrhizae (metabolism)</term>
<term>Organisms, Genetically Modified (MeSH)</term>
<term>Rhizobium (genetics)</term>
<term>Saccharomyces cerevisiae (genetics)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Sequence Analysis, Protein (MeSH)</term>
<term>Transcription, Genetic (MeSH)</term>
<term>Transformation, Genetic (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Fungal Proteins</term>
<term>Metallothionein</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fungal Proteins</term>
<term>Metallothionein</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Metals</term>
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<term>Genetic Complementation Test</term>
<term>Molecular Sequence Data</term>
<term>Organisms, Genetically Modified</term>
<term>Sequence Alignment</term>
<term>Sequence Analysis, Protein</term>
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<term>Transformation, Genetic</term>
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<front><div type="abstract" xml:lang="en">* Metallothioneins are small polypeptides involved in metal tolerance of many eukaryotes. Here we characterized the Pimt1 gene, coding for a metallothionein from the ectomycorrhizal fungus Paxillus involutus. * Expression of Pimt1 in P. involutus under metal stress conditions was measured by northern blot and RT-PCR analyses. The full-length cDNA was used to perform functional complementation in yeast mutant strains and agrotransformation of Hebeloma cylindrosporum. * Heterologous expression in yeast showed that PiMT1 was able to complement the hypersensitivity of mutant strains to cadmium (Cd) and copper (Cu), but not to zinc (Zn). Transcripts were almost undetectable under control conditions, whereas Cu and Cd, but not Zn, strongly induced Pimt1 expression in P. involutus. Constitutive overexpression of Pimt1 in H. cylindrosporum conferred a higher copper tolerance. * The present study identified PiMT1 as a potential determinant in the response of mycorrhizal fungi to Cu and Cd stress. Additionally, we demonstrated the usefulness of mycorrhizal fungi transformation using Agrobacterium technology to approach gene function.</div>
</front>
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<Title>The New phytologist</Title>
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<ArticleTitle>Metal induction of a Paxillus involutus metallothionein and its heterologous expression in Hebeloma cylindrosporum.</ArticleTitle>
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<Abstract><AbstractText>* Metallothioneins are small polypeptides involved in metal tolerance of many eukaryotes. Here we characterized the Pimt1 gene, coding for a metallothionein from the ectomycorrhizal fungus Paxillus involutus. * Expression of Pimt1 in P. involutus under metal stress conditions was measured by northern blot and RT-PCR analyses. The full-length cDNA was used to perform functional complementation in yeast mutant strains and agrotransformation of Hebeloma cylindrosporum. * Heterologous expression in yeast showed that PiMT1 was able to complement the hypersensitivity of mutant strains to cadmium (Cd) and copper (Cu), but not to zinc (Zn). Transcripts were almost undetectable under control conditions, whereas Cu and Cd, but not Zn, strongly induced Pimt1 expression in P. involutus. Constitutive overexpression of Pimt1 in H. cylindrosporum conferred a higher copper tolerance. * The present study identified PiMT1 as a potential determinant in the response of mycorrhizal fungi to Cu and Cd stress. Additionally, we demonstrated the usefulness of mycorrhizal fungi transformation using Agrobacterium technology to approach gene function.</AbstractText>
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