Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection.
Identifieur interne : 000081 ( Main/Exploration ); précédent : 000080; suivant : 000082Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection.
Auteurs : Nusrat Yasmin [Pakistan] ; Mahjabeen Saleem [Pakistan] ; Mamoona Naz [Pakistan] ; Roquyya Gul [Pakistan] ; Hafiz Muzzammel Rehman [Pakistan]Source :
- BioMed research international [ 2314-6141 ] ; 2017.
Descripteurs français
- KwdFr :
- Antifongiques (composition chimique), Antifongiques (usage thérapeutique), Champignons (effets des médicaments et des substances chimiques), Champignons (pathogénicité), Conformation des protéines (MeSH), Humains (MeSH), Musa (composition chimique), Musa (génétique), Mycoses (microbiologie), Mycoses (traitement médicamenteux), Protéines végétales (composition chimique), Protéines végétales (génétique), Protéines végétales (usage thérapeutique), Régulation de l'expression des gènes végétaux (MeSH), Similitude de séquences d'acides aminés (MeSH), Simulation de docking moléculaire (MeSH), Séquence d'acides aminés (MeSH).
- MESH :
- composition chimique : Antifongiques, Musa, Protéines végétales.
- effets des médicaments et des substances chimiques : Champignons.
- génétique : Musa, Protéines végétales.
- microbiologie : Mycoses.
- pathogénicité : Champignons.
- traitement médicamenteux : Mycoses.
- usage thérapeutique : Antifongiques, Protéines végétales.
- Conformation des protéines, Humains, Régulation de l'expression des gènes végétaux, Similitude de séquences d'acides aminés, Simulation de docking moléculaire, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Antifungal Agents (chemistry), Antifungal Agents (therapeutic use), Fungi (drug effects), Fungi (pathogenicity), Gene Expression Regulation, Plant (MeSH), Humans (MeSH), Molecular Docking Simulation (MeSH), Musa (chemistry), Musa (genetics), Mycoses (drug therapy), Mycoses (microbiology), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (therapeutic use), Protein Conformation (MeSH), Sequence Homology, Amino Acid (MeSH).
- MESH :
- chemical , chemistry : Antifungal Agents, Plant Proteins.
- chemical , genetics : Plant Proteins.
- chemical , therapeutic use : Antifungal Agents, Plant Proteins.
- chemistry : Musa.
- drug effects : Fungi.
- drug therapy : Mycoses.
- genetics : Musa.
- microbiology : Mycoses.
- pathogenicity : Fungi.
- Amino Acid Sequence, Gene Expression Regulation, Plant, Humans, Molecular Docking Simulation, Protein Conformation, Sequence Homology, Amino Acid.
Abstract
A thaumatin-like protein gene from Basrai banana was cloned and expressed in Escherichia coli. Amplified gene product was cloned into pTZ57R/T vector and subcloned into expression vector pET22b(+) and resulting pET22b-basrai TLP construct was introduced into E. coli BL21. Maximum protein expression was obtained at 0.7 mM IPTG concentration after 6 hours at 37°C. Western blot analysis showed the presence of approximately 20 kDa protein in induced cells. Basrai antifungal TLP was tried as pharmacological agent against fungal disease. Independently Basrai antifungal protein and amphotericin B exhibited their antifungal activity against A. fumigatus; however combined effect of both agents maximized activity against the pathogen. Docking studies were performed to evaluate the antimicrobial potential of TLP against A. fumigatus by probing binding pattern of antifungal protein with plasma membrane ergosterol of targeted fungal strain. Ice crystallization primarily damages frozen food items; however addition of antifreeze proteins limits the growth of ice crystal in frozen foods. The potential of Basrai TLP protein, as an antifreezing agent, in controlling the ice crystal formation in frozen yogurt was also studied. The scope of this study ranges from cost effective production of pharmaceutics to antifreezing and food preserving agent as well as other real life applications.
DOI: 10.1155/2017/5046451
PubMed: 28875151
PubMed Central: PMC5569749
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<term>Antifungal Agents (therapeutic use)</term>
<term>Fungi (drug effects)</term>
<term>Fungi (pathogenicity)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Molecular Docking Simulation (MeSH)</term>
<term>Musa (chemistry)</term>
<term>Musa (genetics)</term>
<term>Mycoses (drug therapy)</term>
<term>Mycoses (microbiology)</term>
<term>Plant Proteins (chemistry)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (therapeutic use)</term>
<term>Protein Conformation (MeSH)</term>
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<term>Antifongiques (usage thérapeutique)</term>
<term>Champignons (effets des médicaments et des substances chimiques)</term>
<term>Champignons (pathogénicité)</term>
<term>Conformation des protéines (MeSH)</term>
<term>Humains (MeSH)</term>
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<term>Protéines végétales (usage thérapeutique)</term>
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<term>Similitude de séquences d'acides aminés (MeSH)</term>
<term>Simulation de docking moléculaire (MeSH)</term>
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<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Mycoses</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Mycoses</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Fungi</term>
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<front><div type="abstract" xml:lang="en">A thaumatin-like protein gene from Basrai banana was cloned and expressed in <i>Escherichia coli</i>
. Amplified gene product was cloned into pTZ57R/T vector and subcloned into expression vector pET22b(+) and resulting pET22b-basrai TLP construct was introduced into <i>E. coli</i>
BL21. Maximum protein expression was obtained at 0.7 mM IPTG concentration after 6 hours at 37°C. Western blot analysis showed the presence of approximately 20 kDa protein in induced cells. Basrai antifungal TLP was tried as pharmacological agent against fungal disease. Independently Basrai antifungal protein and amphotericin B exhibited their antifungal activity against <i>A. fumigatus</i>
; however combined effect of both agents maximized activity against the pathogen. Docking studies were performed to evaluate the antimicrobial potential of TLP against <i>A. fumigatus</i>
by probing binding pattern of antifungal protein with plasma membrane ergosterol of targeted fungal strain. Ice crystallization primarily damages frozen food items; however addition of antifreeze proteins limits the growth of ice crystal in frozen foods. The potential of Basrai TLP protein, as an antifreezing agent, in controlling the ice crystal formation in frozen yogurt was also studied. The scope of this study ranges from cost effective production of pharmaceutics to antifreezing and food preserving agent as well as other real life applications.</div>
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<Abstract><AbstractText>A thaumatin-like protein gene from Basrai banana was cloned and expressed in <i>Escherichia coli</i>
. Amplified gene product was cloned into pTZ57R/T vector and subcloned into expression vector pET22b(+) and resulting pET22b-basrai TLP construct was introduced into <i>E. coli</i>
BL21. Maximum protein expression was obtained at 0.7 mM IPTG concentration after 6 hours at 37°C. Western blot analysis showed the presence of approximately 20 kDa protein in induced cells. Basrai antifungal TLP was tried as pharmacological agent against fungal disease. Independently Basrai antifungal protein and amphotericin B exhibited their antifungal activity against <i>A. fumigatus</i>
; however combined effect of both agents maximized activity against the pathogen. Docking studies were performed to evaluate the antimicrobial potential of TLP against <i>A. fumigatus</i>
by probing binding pattern of antifungal protein with plasma membrane ergosterol of targeted fungal strain. Ice crystallization primarily damages frozen food items; however addition of antifreeze proteins limits the growth of ice crystal in frozen foods. The potential of Basrai TLP protein, as an antifreezing agent, in controlling the ice crystal formation in frozen yogurt was also studied. The scope of this study ranges from cost effective production of pharmaceutics to antifreezing and food preserving agent as well as other real life applications.</AbstractText>
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