Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection.
Identifieur interne : 000081 ( Main/Exploration ); précédent : 000080; suivant : 000082Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection.
Auteurs : Nusrat Yasmin [Pakistan] ; Mahjabeen Saleem [Pakistan] ; Mamoona Naz [Pakistan] ; Roquyya Gul [Pakistan] ; Hafiz Muzzammel Rehman [Pakistan]Source :
- BioMed research international [ 2314-6141 ] ; 2017.
Descripteurs français
- KwdFr :
- Antifongiques (composition chimique), Antifongiques (usage thérapeutique), Champignons (effets des médicaments et des substances chimiques), Champignons (pathogénicité), Conformation des protéines (MeSH), Humains (MeSH), Musa (composition chimique), Musa (génétique), Mycoses (microbiologie), Mycoses (traitement médicamenteux), Protéines végétales (composition chimique), Protéines végétales (génétique), Protéines végétales (usage thérapeutique), Régulation de l'expression des gènes végétaux (MeSH), Similitude de séquences d'acides aminés (MeSH), Simulation de docking moléculaire (MeSH), Séquence d'acides aminés (MeSH).
- MESH :
- composition chimique : Antifongiques, Musa, Protéines végétales.
- effets des médicaments et des substances chimiques : Champignons.
- génétique : Musa, Protéines végétales.
- microbiologie : Mycoses.
- pathogénicité : Champignons.
- traitement médicamenteux : Mycoses.
- usage thérapeutique : Antifongiques, Protéines végétales.
- Conformation des protéines, Humains, Régulation de l'expression des gènes végétaux, Similitude de séquences d'acides aminés, Simulation de docking moléculaire, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Antifungal Agents (chemistry), Antifungal Agents (therapeutic use), Fungi (drug effects), Fungi (pathogenicity), Gene Expression Regulation, Plant (MeSH), Humans (MeSH), Molecular Docking Simulation (MeSH), Musa (chemistry), Musa (genetics), Mycoses (drug therapy), Mycoses (microbiology), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (therapeutic use), Protein Conformation (MeSH), Sequence Homology, Amino Acid (MeSH).
- MESH :
- chemical , chemistry : Antifungal Agents, Plant Proteins.
- chemical , genetics : Plant Proteins.
- chemical , therapeutic use : Antifungal Agents, Plant Proteins.
- chemistry : Musa.
- drug effects : Fungi.
- drug therapy : Mycoses.
- genetics : Musa.
- microbiology : Mycoses.
- pathogenicity : Fungi.
- Amino Acid Sequence, Gene Expression Regulation, Plant, Humans, Molecular Docking Simulation, Protein Conformation, Sequence Homology, Amino Acid.
Abstract
A thaumatin-like protein gene from Basrai banana was cloned and expressed in Escherichia coli. Amplified gene product was cloned into pTZ57R/T vector and subcloned into expression vector pET22b(+) and resulting pET22b-basrai TLP construct was introduced into E. coli BL21. Maximum protein expression was obtained at 0.7 mM IPTG concentration after 6 hours at 37°C. Western blot analysis showed the presence of approximately 20 kDa protein in induced cells. Basrai antifungal TLP was tried as pharmacological agent against fungal disease. Independently Basrai antifungal protein and amphotericin B exhibited their antifungal activity against A. fumigatus; however combined effect of both agents maximized activity against the pathogen. Docking studies were performed to evaluate the antimicrobial potential of TLP against A. fumigatus by probing binding pattern of antifungal protein with plasma membrane ergosterol of targeted fungal strain. Ice crystallization primarily damages frozen food items; however addition of antifreeze proteins limits the growth of ice crystal in frozen foods. The potential of Basrai TLP protein, as an antifreezing agent, in controlling the ice crystal formation in frozen yogurt was also studied. The scope of this study ranges from cost effective production of pharmaceutics to antifreezing and food preserving agent as well as other real life applications.
DOI: 10.1155/2017/5046451
PubMed: 28875151
PubMed Central: PMC5569749
Affiliations:
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Conformation (MeSH)</term><term>Sequence Homology, Amino Acid (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antifongiques (composition chimique)</term><term>Antifongiques (usage thérapeutique)</term><term>Champignons (effets des médicaments et des substances chimiques)</term><term>Champignons (pathogénicité)</term><term>Conformation des protéines (MeSH)</term><term>Humains (MeSH)</term><term>Musa (composition chimique)</term><term>Musa (génétique)</term><term>Mycoses (microbiologie)</term><term>Mycoses (traitement médicamenteux)</term><term>Protéines végétales (composition chimique)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (usage thérapeutique)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Simulation de docking moléculaire (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antifungal Agents</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Antifungal Agents</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Musa</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Antifongiques</term><term>Musa</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Mycoses</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Champignons</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Musa</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Musa</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Mycoses</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Mycoses</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Champignons</term></keywords><keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Mycoses</term></keywords><keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Antifongiques</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Gene Expression Regulation, Plant</term><term>Humans</term><term>Molecular Docking Simulation</term><term>Protein Conformation</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Conformation des protéines</term><term>Humains</term><term>Régulation de l'expression des gènes végétaux</term><term>Similitude de séquences d'acides aminés</term><term>Simulation de docking moléculaire</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A thaumatin-like protein gene from Basrai banana was cloned and expressed in <i>Escherichia coli</i>. Amplified gene product was cloned into pTZ57R/T vector and subcloned into expression vector pET22b(+) and resulting pET22b-basrai TLP construct was introduced into <i>E. coli</i> BL21. Maximum protein expression was obtained at 0.7 mM IPTG concentration after 6 hours at 37°C. Western blot analysis showed the presence of approximately 20 kDa protein in induced cells. Basrai antifungal TLP was tried as pharmacological agent against fungal disease. Independently Basrai antifungal protein and amphotericin B exhibited their antifungal activity against <i>A. fumigatus</i>; however combined effect of both agents maximized activity against the pathogen. Docking studies were performed to evaluate the antimicrobial potential of TLP against <i>A. fumigatus</i> by probing binding pattern of antifungal protein with plasma membrane ergosterol of targeted fungal strain. Ice crystallization primarily damages frozen food items; however addition of antifreeze proteins limits the growth of ice crystal in frozen foods. The potential of Basrai TLP protein, as an antifreezing agent, in controlling the ice crystal formation in frozen yogurt was also studied. The scope of this study ranges from cost effective production of pharmaceutics to antifreezing and food preserving agent as well as other real life applications.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28875151</PMID><DateCompleted><Year>2018</Year><Month>07</Month><Day>05</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2314-6141</ISSN><JournalIssue CitedMedium="Internet"><Volume>2017</Volume><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>BioMed research international</Title><ISOAbbreviation>Biomed Res Int</ISOAbbreviation></Journal><ArticleTitle>Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection.</ArticleTitle><Pagination><MedlinePgn>5046451</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1155/2017/5046451</ELocationID><Abstract><AbstractText>A thaumatin-like protein gene from Basrai banana was cloned and expressed in <i>Escherichia coli</i>. Amplified gene product was cloned into pTZ57R/T vector and subcloned into expression vector pET22b(+) and resulting pET22b-basrai TLP construct was introduced into <i>E. coli</i> BL21. Maximum protein expression was obtained at 0.7 mM IPTG concentration after 6 hours at 37°C. Western blot analysis showed the presence of approximately 20 kDa protein in induced cells. Basrai antifungal TLP was tried as pharmacological agent against fungal disease. Independently Basrai antifungal protein and amphotericin B exhibited their antifungal activity against <i>A. fumigatus</i>; however combined effect of both agents maximized activity against the pathogen. Docking studies were performed to evaluate the antimicrobial potential of TLP against <i>A. fumigatus</i> by probing binding pattern of antifungal protein with plasma membrane ergosterol of targeted fungal strain. Ice crystallization primarily damages frozen food items; however addition of antifreeze proteins limits the growth of ice crystal in frozen foods. The potential of Basrai TLP protein, as an antifreezing agent, in controlling the ice crystal formation in frozen yogurt was also studied. The scope of this study ranges from cost effective production of pharmaceutics to antifreezing and food preserving agent as well as other real life applications.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yasmin</LastName><ForeName>Nusrat</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore 54590, Pakistan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saleem</LastName><ForeName>Mahjabeen</ForeName><Initials>M</Initials><Identifier Source="ORCID">0000-0001-8944-8714</Identifier><AffiliationInfo><Affiliation>Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore 54590, Pakistan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Naz</LastName><ForeName>Mamoona</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore 54590, Pakistan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gul</LastName><ForeName>Roquyya</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>The University of Lahore, Lahore 54590, Pakistan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rehman</LastName><ForeName>Hafiz Muzzammel</ForeName><Initials>HM</Initials><AffiliationInfo><Affiliation>Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore 54590, Pakistan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>08</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biomed Res Int</MedlineTA><NlmUniqueID>101600173</NlmUniqueID></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000935">Antifungal Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C420118">thaumatin-like protein, Musa acuminata</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000935" MajorTopicYN="N">Antifungal Agents</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000627" MajorTopicYN="N">therapeutic use</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D062105" MajorTopicYN="N">Molecular Docking Simulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D028521" MajorTopicYN="N">Musa</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009181" MajorTopicYN="N">Mycoses</DescriptorName><QualifierName UI="Q000188" MajorTopicYN="Y">drug therapy</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000627" MajorTopicYN="N">therapeutic use</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011487" MajorTopicYN="N">Protein Conformation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>05</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>07</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>9</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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sortKey="Gul, Roquyya" sort="Gul, Roquyya" uniqKey="Gul R" first="Roquyya" last="Gul">Roquyya Gul</name><name sortKey="Naz, Mamoona" sort="Naz, Mamoona" uniqKey="Naz M" first="Mamoona" last="Naz">Mamoona Naz</name><name sortKey="Rehman, Hafiz Muzzammel" sort="Rehman, Hafiz Muzzammel" uniqKey="Rehman H" first="Hafiz Muzzammel" last="Rehman">Hafiz Muzzammel Rehman</name><name sortKey="Saleem, Mahjabeen" sort="Saleem, Mahjabeen" uniqKey="Saleem M" first="Mahjabeen" last="Saleem">Mahjabeen Saleem</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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