Cloning and expression of three thaumatin-like protein genes from Polyporus umbellatus.
Identifieur interne : 000095 ( Main/Exploration ); précédent : 000094; suivant : 000096Cloning and expression of three thaumatin-like protein genes from Polyporus umbellatus.
Auteurs : Mengmeng Liu [République populaire de Chine] ; Dawei Zhang [République populaire de Chine] ; Yongmei Xing [République populaire de Chine] ; Shunxing Guo [République populaire de Chine]Source :
- Acta pharmaceutica Sinica. B [ 2211-3835 ] ; 2017.
Abstract
Genes encoding thaumatin-like protein (TLPs) are frequently found in fungal genomes. However, information on TLP genes in Polyporus umbellatus is still limited. In this study, three TLP genes were cloned from P. umbellatus. The full-length coding sequence of PuTLP1, PuTLP2 and PuTLP3 were 768, 759 and 561 bp long, respectively, encoding for 256, 253 and 187 amino acids. Phylogenetic trees showed that P. umbellatus PuTLP1, PuTLP2 and PuTLP3 were clustered with sequences from Gloeophyllum trabeum, Trametes versicolor and Stereum hirsutum, respectively. The expression patterns of the three TLP genes were higher in P. umbellatus with Armillaria mellea infection than in the sclerotia without A. mellea. Furthermore, over-expression of three PuTLPs were carried out in Escherichia coli BL21 (DE3) strain, and high quality proteins were obtained using Ni-NTA resin that can be used for preparation of specific antibodies. These results suggest that PuTLP1, PuTLP2 and PuTLP3 in P. umbellatus may be involved in the defense response to A. mellea infections.
DOI: 10.1016/j.apsb.2017.03.001
PubMed: 28540175
PubMed Central: PMC5430889
Affiliations:
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<front><div type="abstract" xml:lang="en">Genes encoding thaumatin-like protein (<i>TLPs</i>
) are frequently found in fungal genomes. However, information on <i>TLP</i>
genes in <i>Polyporus umbellatus</i>
is still limited. In this study, three <i>TLP</i>
genes were cloned from <i>P. umbellatus</i>
. The full-length coding sequence of <i>PuTLP1</i>
, <i>PuTLP2</i>
and <i>PuTLP3</i>
were 768, 759 and 561 bp long, respectively, encoding for 256, 253 and 187 amino acids. Phylogenetic trees showed that <i>P. umbellatus PuTLP1</i>
, <i>PuTLP2</i>
and <i>PuTLP3</i>
were clustered with sequences from <i>Gloeophyllum trabeum, Trametes versicolor</i>
and <i>Stereum hirsutum</i>
, respectively. The expression patterns of the three <i>TLP</i>
genes were higher in <i>P. umbellatus</i>
with <i>Armillaria mellea</i>
infection than in the sclerotia without <i>A. mellea</i>
. Furthermore, over-expression of three PuTLPs were carried out in <i>Escherichia coli</i>
BL21 (DE3) strain, and high quality proteins were obtained using Ni-NTA resin that can be used for preparation of specific antibodies. These results suggest that <i>PuTLP1</i>
, <i>PuTLP2</i>
and <i>PuTLP3</i>
in <i>P. umbellatus</i>
may be involved in the defense response to <i>A. mellea</i>
infections.</div>
</front>
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<Title>Acta pharmaceutica Sinica. B</Title>
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<ArticleTitle>Cloning and expression of three thaumatin-like protein genes from <i>Polyporus umbellatus</i>
.</ArticleTitle>
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<Abstract><AbstractText>Genes encoding thaumatin-like protein (<i>TLPs</i>
) are frequently found in fungal genomes. However, information on <i>TLP</i>
genes in <i>Polyporus umbellatus</i>
is still limited. In this study, three <i>TLP</i>
genes were cloned from <i>P. umbellatus</i>
. The full-length coding sequence of <i>PuTLP1</i>
, <i>PuTLP2</i>
and <i>PuTLP3</i>
were 768, 759 and 561 bp long, respectively, encoding for 256, 253 and 187 amino acids. Phylogenetic trees showed that <i>P. umbellatus PuTLP1</i>
, <i>PuTLP2</i>
and <i>PuTLP3</i>
were clustered with sequences from <i>Gloeophyllum trabeum, Trametes versicolor</i>
and <i>Stereum hirsutum</i>
, respectively. The expression patterns of the three <i>TLP</i>
genes were higher in <i>P. umbellatus</i>
with <i>Armillaria mellea</i>
infection than in the sclerotia without <i>A. mellea</i>
. Furthermore, over-expression of three PuTLPs were carried out in <i>Escherichia coli</i>
BL21 (DE3) strain, and high quality proteins were obtained using Ni-NTA resin that can be used for preparation of specific antibodies. These results suggest that <i>PuTLP1</i>
, <i>PuTLP2</i>
and <i>PuTLP3</i>
in <i>P. umbellatus</i>
may be involved in the defense response to <i>A. mellea</i>
infections.</AbstractText>
</Abstract>
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<AffiliationInfo><Affiliation>Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213001, China.</Affiliation>
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<AffiliationInfo><Affiliation>Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213001, China.</Affiliation>
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<Initials>S</Initials>
<AffiliationInfo><Affiliation>Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.</Affiliation>
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<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Defense</Keyword>
<Keyword MajorTopicYN="N">Expression</Keyword>
<Keyword MajorTopicYN="N">Gene cloning</Keyword>
<Keyword MajorTopicYN="N">Polyporus umbellatus</Keyword>
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<ReferenceList><Reference><Citation>FEMS Microbiol Rev. 2007 Mar;31(2):168-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17313520</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Rep. 2015 Jun 01;5:10759</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26030006</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods. 2001 Dec;25(4):402-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11846609</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Phytopathol. 2006;44:135-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16602946</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2006 Jun;141(2):793-801</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16648221</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Evol. 2013 Dec;30(12):2725-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24132122</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Pathol. 2015 Feb;16(2):123-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24965864</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Rep. 2010 May;29(5):419-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20204373</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Evol. 2006 Jul;63(1):12-29</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16736102</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Rep. 2015 Nov 03;5:16075</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26526032</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Plant Biol. 2011 Feb 15;11:33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21324123</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Mol Sci. 2014 Sep 10;15(9):15951-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25210845</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2015 Oct 30;466(4):629-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26367180</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Rep. 2016 May 06;6:25340</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27150014</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1994 Nov 11;22(22):4673-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7984417</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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</country>
<settlement><li>Pékin</li>
</settlement>
</list>
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