Cloning and expression of three thaumatin-like protein genes from Polyporus umbellatus.
Identifieur interne : 000095 ( Main/Exploration ); précédent : 000094; suivant : 000096Cloning and expression of three thaumatin-like protein genes from Polyporus umbellatus.
Auteurs : Mengmeng Liu [République populaire de Chine] ; Dawei Zhang [République populaire de Chine] ; Yongmei Xing [République populaire de Chine] ; Shunxing Guo [République populaire de Chine]Source :
- Acta pharmaceutica Sinica. B [ 2211-3835 ] ; 2017.
Abstract
Genes encoding thaumatin-like protein (TLPs) are frequently found in fungal genomes. However, information on TLP genes in Polyporus umbellatus is still limited. In this study, three TLP genes were cloned from P. umbellatus. The full-length coding sequence of PuTLP1, PuTLP2 and PuTLP3 were 768, 759 and 561 bp long, respectively, encoding for 256, 253 and 187 amino acids. Phylogenetic trees showed that P. umbellatus PuTLP1, PuTLP2 and PuTLP3 were clustered with sequences from Gloeophyllum trabeum, Trametes versicolor and Stereum hirsutum, respectively. The expression patterns of the three TLP genes were higher in P. umbellatus with Armillaria mellea infection than in the sclerotia without A. mellea. Furthermore, over-expression of three PuTLPs were carried out in Escherichia coli BL21 (DE3) strain, and high quality proteins were obtained using Ni-NTA resin that can be used for preparation of specific antibodies. These results suggest that PuTLP1, PuTLP2 and PuTLP3 in P. umbellatus may be involved in the defense response to A. mellea infections.
DOI: 10.1016/j.apsb.2017.03.001
PubMed: 28540175
PubMed Central: PMC5430889
Affiliations:
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xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213001</wicri:regionArea><wicri:noRegion>Changzhou 213001</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Dawei" sort="Zhang, Dawei" uniqKey="Zhang D" first="Dawei" last="Zhang">Dawei Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213001, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213001</wicri:regionArea><wicri:noRegion>Changzhou 213001</wicri:noRegion></affiliation></author><author><name sortKey="Xing, Yongmei" sort="Xing, Yongmei" uniqKey="Xing Y" first="Yongmei" last="Xing">Yongmei Xing</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Guo, Shunxing" sort="Guo, Shunxing" uniqKey="Guo S" first="Shunxing" last="Guo">Shunxing Guo</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Acta pharmaceutica Sinica. B</title><idno type="ISSN">2211-3835</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Genes encoding thaumatin-like protein (<i>TLPs</i>) are frequently found in fungal genomes. However, information on <i>TLP</i> genes in <i>Polyporus umbellatus</i> is still limited. In this study, three <i>TLP</i> genes were cloned from <i>P. umbellatus</i>. The full-length coding sequence of <i>PuTLP1</i>, <i>PuTLP2</i> and <i>PuTLP3</i> were 768, 759 and 561 bp long, respectively, encoding for 256, 253 and 187 amino acids. Phylogenetic trees showed that <i>P. umbellatus PuTLP1</i>, <i>PuTLP2</i> and <i>PuTLP3</i> were clustered with sequences from <i>Gloeophyllum trabeum, Trametes versicolor</i> and <i>Stereum hirsutum</i>, respectively. The expression patterns of the three <i>TLP</i> genes were higher in <i>P. umbellatus</i> with <i>Armillaria mellea</i> infection than in the sclerotia without <i>A. mellea</i>. Furthermore, over-expression of three PuTLPs were carried out in <i>Escherichia coli</i> BL21 (DE3) strain, and high quality proteins were obtained using Ni-NTA resin that can be used for preparation of specific antibodies. These results suggest that <i>PuTLP1</i>, <i>PuTLP2</i> and <i>PuTLP3</i> in <i>P. umbellatus</i> may be involved in the defense response to <i>A. mellea</i> infections.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">28540175</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">2211-3835</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>3</Issue><PubDate><Year>2017</Year><Month>May</Month></PubDate></JournalIssue><Title>Acta pharmaceutica Sinica. B</Title><ISOAbbreviation>Acta Pharm Sin B</ISOAbbreviation></Journal><ArticleTitle>Cloning and expression of three thaumatin-like protein genes from <i>Polyporus umbellatus</i>.</ArticleTitle><Pagination><MedlinePgn>373-380</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.apsb.2017.03.001</ELocationID><Abstract><AbstractText>Genes encoding thaumatin-like protein (<i>TLPs</i>) are frequently found in fungal genomes. However, information on <i>TLP</i> genes in <i>Polyporus umbellatus</i> is still limited. In this study, three <i>TLP</i> genes were cloned from <i>P. umbellatus</i>. The full-length coding sequence of <i>PuTLP1</i>, <i>PuTLP2</i> and <i>PuTLP3</i> were 768, 759 and 561 bp long, respectively, encoding for 256, 253 and 187 amino acids. Phylogenetic trees showed that <i>P. umbellatus PuTLP1</i>, <i>PuTLP2</i> and <i>PuTLP3</i> were clustered with sequences from <i>Gloeophyllum trabeum, Trametes versicolor</i> and <i>Stereum hirsutum</i>, respectively. The expression patterns of the three <i>TLP</i> genes were higher in <i>P. umbellatus</i> with <i>Armillaria mellea</i> infection than in the sclerotia without <i>A. mellea</i>. Furthermore, over-expression of three PuTLPs were carried out in <i>Escherichia coli</i> BL21 (DE3) strain, and high quality proteins were obtained using Ni-NTA resin that can be used for preparation of specific antibodies. These results suggest that <i>PuTLP1</i>, <i>PuTLP2</i> and <i>PuTLP3</i> in <i>P. umbellatus</i> may be involved in the defense response to <i>A. mellea</i> infections.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Mengmeng</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Dawei</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213001, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xing</LastName><ForeName>Yongmei</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Shunxing</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>04</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Acta Pharm Sin B</MedlineTA><NlmUniqueID>101600560</NlmUniqueID><ISSNLinking>2211-3835</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Defense</Keyword><Keyword MajorTopicYN="N">Expression</Keyword><Keyword MajorTopicYN="N">Gene cloning</Keyword><Keyword MajorTopicYN="N">Polyporus umbellatus</Keyword><Keyword MajorTopicYN="N">Thaumatin-like protein</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>02</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>03</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>03</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>5</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>5</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>5</Month><Day>26</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28540175</ArticleId><ArticleId 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Shunxing" uniqKey="Guo S" first="Shunxing" last="Guo">Shunxing Guo</name><name sortKey="Liu, Mengmeng" sort="Liu, Mengmeng" uniqKey="Liu M" first="Mengmeng" last="Liu">Mengmeng Liu</name><name sortKey="Xing, Yongmei" sort="Xing, Yongmei" uniqKey="Xing Y" first="Yongmei" last="Xing">Yongmei Xing</name><name sortKey="Zhang, Dawei" sort="Zhang, Dawei" uniqKey="Zhang D" first="Dawei" last="Zhang">Dawei Zhang</name><name sortKey="Zhang, Dawei" sort="Zhang, Dawei" uniqKey="Zhang D" first="Dawei" last="Zhang">Dawei Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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