In vitro and in vivo characterization of hazelnut skin prick test extracts.
Identifieur interne : 000466 ( Main/Exploration ); précédent : 000465; suivant : 000467In vitro and in vivo characterization of hazelnut skin prick test extracts.
Auteurs : Jaap H. Akkerdaas ; Marjolein Wensing ; André C. Knulst ; Rob C. Aalberse ; Susan L. Hefle ; Ronald Van ReeSource :
- Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M [ 0936-8671 ] ; 2003.
Descripteurs français
- KwdFr :
- MESH :
- composition chimique : Noix.
- diagnostic : Hypersensibilité.
- immunologie : Noix.
- Extraits de plantes, Humains, Reproductibilité des résultats, Techniques in vitro, Tests cutanés.
English descriptors
- KwdEn :
- MESH :
- chemical : Plant Extracts.
- chemistry : Nuts.
- diagnosis : Hypersensitivity.
- immunology : Nuts.
- Humans, In Vitro Techniques, Reproducibility of Results, Skin Tests.
Abstract
RATIONALE
Hazelnut allergy ranks among the most frequently observed food allergies. Clinical symptoms range from the oral allergy syndrome to life threatening anaphylaxis. Diagnosis of hazelnut allergy partially relies on in vivo testing by means of skin prick testing (SPT). The aim of this study was to characterize hazelnut SPT extracts both in vitro and in vivo.
METHODS
Hazelnut SPT extracts were investigated for protein concentration and composition. The major hazelnut allergen Cor a 1, lipid transfer protein (LTP) and thaumatin-like-protein (TLP) were monitored by competitive RIA and immunoblotting. SPT extracts (n = 6) were analyzed for skin reactivity and the correlation between the SPT extract protein concentration and the mean skin reactivity (HEIC) was determined in a group of hazelnut-allergic patients (n = 30). For one SPT extract, the threshold level for Cor a 1 was determined in Cor a 1-monosensitized patients (n = 5).
RESULTS
Protein concentrations ranged from 0.2-14 mg/ml. Although some proteins were present in most extracts (bands at 10, 22-28, 32 and around 48 kDa), clear differences in composition were observed (both intra- and inter-variability). The concentration of the major hazelnut allergen Cor a 1 differed up to a factor 50 (0.6-32 micrograms/ml). LTP was virtually absent in 3/9 SPT extracts and variable quantities of TLP were detected by immunoblotting. Some patients (6/30) had a false-negative SPT with 3/6 SPT extracts. There was a clear correlation between the protein concentration and the mean HEIC (RPearson = 0.87). The threshold level for Cor a 1 was +/- 3.2 ng/ml as assessed with one of the products investigated.
CONCLUSIONS
Heterogeneous protein concentration/composition of SPT extracts results in variable skin test responses. The absence of potentially severe allergens like LTP may lead to false-negative SPT results that jeopardize a patient's safety. From these results it can be concluded that there is a strong need for standardization of products for SPT.
PubMed: 15119025
Affiliations:
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Aalberse</name></author><author><name sortKey="Hefle, Susan L" sort="Hefle, Susan L" uniqKey="Hefle S" first="Susan L" last="Hefle">Susan L. Hefle</name></author><author><name sortKey="Van Ree, Ronald" sort="Van Ree, Ronald" uniqKey="Van Ree R" first="Ronald" last="Van Ree">Ronald Van Ree</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:15119025</idno><idno type="pmid">15119025</idno><idno type="wicri:Area/Main/Corpus">000447</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000447</idno><idno type="wicri:Area/Main/Curation">000447</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000447</idno><idno type="wicri:Area/Main/Exploration">000447</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">In vitro and in vivo characterization of hazelnut skin prick test extracts.</title><author><name sortKey="Akkerdaas, Jaap H" sort="Akkerdaas, Jaap H" uniqKey="Akkerdaas J" first="Jaap H" last="Akkerdaas">Jaap H. Akkerdaas</name><affiliation><nlm:affiliation>CLB Central Lab. of The Netherlands, Red Cross Blood Transfusion Service, Plesmanlaan 125, NL-1066 CX Amsterdam.</nlm:affiliation><wicri:noCountry code="subField">NL-1066 CX Amsterdam</wicri:noCountry></affiliation></author><author><name sortKey="Wensing, Marjolein" sort="Wensing, Marjolein" uniqKey="Wensing M" first="Marjolein" last="Wensing">Marjolein Wensing</name></author><author><name sortKey="Knulst, Andre C" sort="Knulst, Andre C" uniqKey="Knulst A" first="André C" last="Knulst">André C. Knulst</name></author><author><name sortKey="Aalberse, Rob C" sort="Aalberse, Rob C" uniqKey="Aalberse R" first="Rob C" last="Aalberse">Rob C. Aalberse</name></author><author><name sortKey="Hefle, Susan L" sort="Hefle, Susan L" uniqKey="Hefle S" first="Susan L" last="Hefle">Susan L. Hefle</name></author><author><name sortKey="Van Ree, Ronald" sort="Van Ree, Ronald" uniqKey="Van Ree R" first="Ronald" last="Van Ree">Ronald Van Ree</name></author></analytic><series><title level="j">Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M</title><idno type="ISSN">0936-8671</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Humans (MeSH)</term><term>Hypersensitivity (diagnosis)</term><term>In Vitro Techniques (MeSH)</term><term>Nuts (chemistry)</term><term>Nuts (immunology)</term><term>Plant Extracts (MeSH)</term><term>Reproducibility of Results (MeSH)</term><term>Skin Tests (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Extraits de plantes (MeSH)</term><term>Humains (MeSH)</term><term>Hypersensibilité (diagnostic)</term><term>Noix (composition chimique)</term><term>Noix (immunologie)</term><term>Reproductibilité des résultats (MeSH)</term><term>Techniques in vitro (MeSH)</term><term>Tests cutanés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Plant Extracts</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nuts</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Noix</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Hypersensitivity</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Hypersensibilité</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Noix</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Nuts</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>In Vitro Techniques</term><term>Reproducibility of Results</term><term>Skin Tests</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Extraits de plantes</term><term>Humains</term><term>Reproductibilité des résultats</term><term>Techniques in vitro</term><term>Tests cutanés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>RATIONALE</b></p><p>Hazelnut allergy ranks among the most frequently observed food allergies. Clinical symptoms range from the oral allergy syndrome to life threatening anaphylaxis. Diagnosis of hazelnut allergy partially relies on in vivo testing by means of skin prick testing (SPT). The aim of this study was to characterize hazelnut SPT extracts both in vitro and in vivo.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>Hazelnut SPT extracts were investigated for protein concentration and composition. The major hazelnut allergen Cor a 1, lipid transfer protein (LTP) and thaumatin-like-protein (TLP) were monitored by competitive RIA and immunoblotting. SPT extracts (n = 6) were analyzed for skin reactivity and the correlation between the SPT extract protein concentration and the mean skin reactivity (HEIC) was determined in a group of hazelnut-allergic patients (n = 30). For one SPT extract, the threshold level for Cor a 1 was determined in Cor a 1-monosensitized patients (n = 5).</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Protein concentrations ranged from 0.2-14 mg/ml. Although some proteins were present in most extracts (bands at 10, 22-28, 32 and around 48 kDa), clear differences in composition were observed (both intra- and inter-variability). The concentration of the major hazelnut allergen Cor a 1 differed up to a factor 50 (0.6-32 micrograms/ml). LTP was virtually absent in 3/9 SPT extracts and variable quantities of TLP were detected by immunoblotting. Some patients (6/30) had a false-negative SPT with 3/6 SPT extracts. There was a clear correlation between the protein concentration and the mean HEIC (RPearson = 0.87). The threshold level for Cor a 1 was +/- 3.2 ng/ml as assessed with one of the products investigated.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>Heterogeneous protein concentration/composition of SPT extracts results in variable skin test responses. The absence of potentially severe allergens like LTP may lead to false-negative SPT results that jeopardize a patient's safety. From these results it can be concluded that there is a strong need for standardization of products for SPT.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15119025</PMID><DateCompleted><Year>2004</Year><Month>05</Month><Day>25</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0936-8671</ISSN><JournalIssue CitedMedium="Print"><Issue>94</Issue><PubDate><Year>2003</Year></PubDate></JournalIssue><Title>Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M</Title><ISOAbbreviation>Arb Paul Ehrlich Inst Bundesamt Sera Impfstoffe Frankf A M</ISOAbbreviation></Journal><ArticleTitle>In vitro and in vivo characterization of hazelnut skin prick test extracts.</ArticleTitle><Pagination><MedlinePgn>87-95; discussion 96</MedlinePgn></Pagination><Abstract><AbstractText Label="RATIONALE" NlmCategory="BACKGROUND">Hazelnut allergy ranks among the most frequently observed food allergies. Clinical symptoms range from the oral allergy syndrome to life threatening anaphylaxis. Diagnosis of hazelnut allergy partially relies on in vivo testing by means of skin prick testing (SPT). The aim of this study was to characterize hazelnut SPT extracts both in vitro and in vivo.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Hazelnut SPT extracts were investigated for protein concentration and composition. The major hazelnut allergen Cor a 1, lipid transfer protein (LTP) and thaumatin-like-protein (TLP) were monitored by competitive RIA and immunoblotting. SPT extracts (n = 6) were analyzed for skin reactivity and the correlation between the SPT extract protein concentration and the mean skin reactivity (HEIC) was determined in a group of hazelnut-allergic patients (n = 30). For one SPT extract, the threshold level for Cor a 1 was determined in Cor a 1-monosensitized patients (n = 5).</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Protein concentrations ranged from 0.2-14 mg/ml. Although some proteins were present in most extracts (bands at 10, 22-28, 32 and around 48 kDa), clear differences in composition were observed (both intra- and inter-variability). The concentration of the major hazelnut allergen Cor a 1 differed up to a factor 50 (0.6-32 micrograms/ml). LTP was virtually absent in 3/9 SPT extracts and variable quantities of TLP were detected by immunoblotting. Some patients (6/30) had a false-negative SPT with 3/6 SPT extracts. There was a clear correlation between the protein concentration and the mean HEIC (RPearson = 0.87). The threshold level for Cor a 1 was +/- 3.2 ng/ml as assessed with one of the products investigated.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Heterogeneous protein concentration/composition of SPT extracts results in variable skin test responses. The absence of potentially severe allergens like LTP may lead to false-negative SPT results that jeopardize a patient's safety. From these results it can be concluded that there is a strong need for standardization of products for SPT.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Akkerdaas</LastName><ForeName>Jaap H</ForeName><Initials>JH</Initials><AffiliationInfo><Affiliation>CLB Central Lab. of The Netherlands, Red Cross Blood Transfusion Service, Plesmanlaan 125, NL-1066 CX Amsterdam.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wensing</LastName><ForeName>Marjolein</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Knulst</LastName><ForeName>André C</ForeName><Initials>AC</Initials></Author><Author ValidYN="Y"><LastName>Aalberse</LastName><ForeName>Rob C</ForeName><Initials>RC</Initials></Author><Author ValidYN="Y"><LastName>Hefle</LastName><ForeName>Susan L</ForeName><Initials>SL</Initials></Author><Author ValidYN="Y"><LastName>van Ree</LastName><ForeName>Ronald</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Arb Paul Ehrlich Inst Bundesamt Sera Impfstoffe Frankf A M</MedlineTA><NlmUniqueID>8912864</NlmUniqueID><ISSNLinking>0936-8671</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006967" MajorTopicYN="N">Hypersensitivity</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D066298" MajorTopicYN="N">In Vitro Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009754" MajorTopicYN="N">Nuts</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012882" MajorTopicYN="Y">Skin Tests</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>5</Month><Day>4</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>5</Month><Day>27</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>5</Month><Day>4</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15119025</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Aalberse, Rob C" sort="Aalberse, Rob C" uniqKey="Aalberse R" first="Rob C" last="Aalberse">Rob C. Aalberse</name><name sortKey="Akkerdaas, Jaap H" sort="Akkerdaas, Jaap H" uniqKey="Akkerdaas J" first="Jaap H" last="Akkerdaas">Jaap H. Akkerdaas</name><name sortKey="Hefle, Susan L" sort="Hefle, Susan L" uniqKey="Hefle S" first="Susan L" last="Hefle">Susan L. Hefle</name><name sortKey="Knulst, Andre C" sort="Knulst, Andre C" uniqKey="Knulst A" first="André C" last="Knulst">André C. Knulst</name><name sortKey="Van Ree, Ronald" sort="Van Ree, Ronald" uniqKey="Van Ree R" first="Ronald" last="Van Ree">Ronald Van Ree</name><name sortKey="Wensing, Marjolein" sort="Wensing, Marjolein" uniqKey="Wensing M" first="Marjolein" last="Wensing">Marjolein Wensing</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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