ThaumatinV1, Main, Exploration, bibRecord, 000245

Use of dual polarization interferometry as a diagnostic tool for protein crystallization.

Identifieur interne : 000245 ( Main/Exploration ); précédent : 000244; suivant : 000246

Use of dual polarization interferometry as a diagnostic tool for protein crystallization.

Auteurs : Attia Boudjemline [Royaume-Uni] ; Emmanuel Saridakis ; Marcus J. Swann ; Lata Govada ; Irene M. Mavridis ; Naomi E. Chayen

Source :

Analytical chemistry [ 1520-6882 ] ; 2011.

RBID : pubmed:21894980

Descripteurs français

KwdFr :
- Animaux (MeSH), Catalase (composition chimique), Cristallisation (MeSH), Dynamines (composition chimique), Endo-1,4-beta xylanases (composition chimique), Interférométrie (MeSH), Lasers à gaz (MeSH), Lumière (MeSH), Lysozyme (composition chimique), Protéines (composition chimique), Protéines végétales (composition chimique), Rats (MeSH).
MESH :
- composition chimique : Catalase, Dynamines, Endo-1,4-beta xylanases, Lysozyme, Protéines, Protéines végétales.
- Animaux, Cristallisation, Interférométrie, Lasers à gaz, Lumière, Rats.

English descriptors

KwdEn :
- Animals (MeSH), Catalase (chemistry), Crystallization (MeSH), Dynamins (chemistry), Endo-1,4-beta Xylanases (chemistry), Interferometry (MeSH), Lasers, Gas (MeSH), Light (MeSH), Muramidase (chemistry), Plant Proteins (chemistry), Proteins (chemistry), Rats (MeSH).
MESH :
- chemical , chemistry : Catalase, Dynamins, Endo-1,4-beta Xylanases, Muramidase, Plant Proteins, Proteins.
- Animals, Crystallization, Interferometry, Lasers, Gas, Light, Rats.

Abstract

The use of dual polarization interferometry (DPI) as a tool for probing the different possible outcomes of protein crystallization experiments is described. DPI is a surface analytical technique used for the characterization of structure and interactions of molecular layers on an optical waveguide surface for a wide range of applications, including protein-protein interactions and conformational changes. The application of this technique provides a "signature" of crystallization events, thus predicting if there will be protein crystal formation, amorphous precipitate, or clear solution. The technique was demonstrated on a number of model proteins, and it also produced meaningful results in the case of two problematic target proteins. DPI in conjunction with a dialysis setup, allows changes in the protein solution above the waveguide surface to be monitored simultaneously with continuous control of its precipitant content. DPI has the potential to be used as a powerful method for discovering crystallization conditions, for obtaining information on the crystallization process, and as an aid in crystal optimization. It has also provided what is, to the best of our knowledge, the most direct observation to date of salting-in behavior in a protein-salt solution.

DOI: 10.1021/ac2017844
PubMed: 21894980

Affiliations:

Royaume-Uni

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Le document en format XML

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<term>Endo-1,4-beta Xylanases (chemistry)</term>
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<term>Lasers, Gas (MeSH)</term>
<term>Light (MeSH)</term>
<term>Muramidase (chemistry)</term>
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<term>Catalase (composition chimique)</term>
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<term>Dynamines (composition chimique)</term>
<term>Endo-1,4-beta xylanases (composition chimique)</term>
<term>Interférométrie (MeSH)</term>
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<term>Plant Proteins</term>
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<term>Dynamines</term>
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<term>Lysozyme</term>
<term>Protéines</term>
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<term>Rats</term>
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<front><div type="abstract" xml:lang="en">The use of dual polarization interferometry (DPI) as a tool for probing the different possible outcomes of protein crystallization experiments is described. DPI is a surface analytical technique used for the characterization of structure and interactions of molecular layers on an optical waveguide surface for a wide range of applications, including protein-protein interactions and conformational changes. The application of this technique provides a "signature" of crystallization events, thus predicting if there will be protein crystal formation, amorphous precipitate, or clear solution. The technique was demonstrated on a number of model proteins, and it also produced meaningful results in the case of two problematic target proteins. DPI in conjunction with a dialysis setup, allows changes in the protein solution above the waveguide surface to be monitored simultaneously with continuous control of its precipitant content. DPI has the potential to be used as a powerful method for discovering crystallization conditions, for obtaining information on the crystallization process, and as an aid in crystal optimization. It has also provided what is, to the best of our knowledge, the most direct observation to date of salting-in behavior in a protein-salt solution.</div>
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Use of dual polarization interferometry as a diagnostic tool for protein crystallization.

Use of dual polarization interferometry as a diagnostic tool for protein crystallization.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki