Identification of olive (Olea europaea) seed and pulp proteins by nLC-MS/MS via combinatorial peptide ligand libraries.
Identifieur interne : 000226 ( Main/Exploration ); précédent : 000225; suivant : 000227Identification of olive (Olea europaea) seed and pulp proteins by nLC-MS/MS via combinatorial peptide ligand libraries.
Auteurs : Clara Esteve [Espagne] ; Alfonsina D'Amato ; María Luisa Marina ; María Concepci N García ; Attilio Citterio ; Pier Giorgio RighettiSource :
- Journal of proteomics [ 1876-7737 ] ; 2012.
Descripteurs français
- KwdFr :
- Banque de peptides (MeSH), Chromatographie en phase liquide (méthodes), Fruit (composition chimique), Fruit (métabolisme), Graines (composition chimique), Graines (métabolisme), Ligands (MeSH), Modèles biologiques (MeSH), Olea (composition chimique), Olea (métabolisme), Protéines végétales (analyse), Protéines végétales (métabolisme), Protéomique (méthodes), Spectrométrie de masse en tandem (méthodes), Techniques de chimie combinatoire (méthodes), Électrophorèse sur gel de polyacrylamide (MeSH).
- MESH :
- analyse : Protéines végétales.
- composition chimique : Fruit, Graines, Olea.
- métabolisme : Fruit, Graines, Olea, Protéines végétales.
- méthodes : Chromatographie en phase liquide, Protéomique, Spectrométrie de masse en tandem, Techniques de chimie combinatoire.
- Banque de peptides, Ligands, Modèles biologiques, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Chromatography, Liquid (methods), Combinatorial Chemistry Techniques (methods), Electrophoresis, Polyacrylamide Gel (MeSH), Fruit (chemistry), Fruit (metabolism), Ligands (MeSH), Models, Biological (MeSH), Olea (chemistry), Olea (metabolism), Peptide Library (MeSH), Plant Proteins (analysis), Plant Proteins (metabolism), Proteomics (methods), Seeds (chemistry), Seeds (metabolism), Tandem Mass Spectrometry (methods).
- MESH :
- chemical , analysis : Plant Proteins.
- chemical , metabolism : Plant Proteins.
- chemical : Ligands, Peptide Library.
- chemistry : Fruit, Olea, Seeds.
- metabolism : Fruit, Olea, Seeds.
- methods : Chromatography, Liquid, Combinatorial Chemistry Techniques, Proteomics, Tandem Mass Spectrometry.
- Electrophoresis, Polyacrylamide Gel, Models, Biological.
Abstract
Different types of extraction protocols are described for identifying proteins in seed and pulp of olive (Olea europea), by employing both conventional extraction methods and capture with ProteoMiner as well as with in house-made combinatorial peptide ligand libraries (HM-CPLLs) at pH 7.4 and at pH 2.2. Thanks to the use of CPLLs, able to dramatically amplify the signal of low-abundance species, a quite large number of compounds has been indeed identified: 61 in the seed (vs. only four reported in current literature) and 231 in the pulp (vs. 56 described so far), the deepest investigation up to the present of the olive proteome. In the seed, it highlights the presence of seed storage proteins, oleosins and histones. In the pulp, the allergenic thaumatin-like protein (Ole e 13) was confirmed, among the other 231, as the most abundant protein in the olive pulp. The present research has also been undertaken with the aim of identifying proteins in olive oil and ascertaining the relative contribution of seed and pulp proteins in their presence, if any, in oils.
DOI: 10.1016/j.jprot.2012.02.020
PubMed: 22387115
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Thanks to the use of CPLLs, able to dramatically amplify the signal of low-abundance species, a quite large number of compounds has been indeed identified: 61 in the seed (vs. only four reported in current literature) and 231 in the pulp (vs. 56 described so far), the deepest investigation up to the present of the olive proteome. In the seed, it highlights the presence of seed storage proteins, oleosins and histones. In the pulp, the allergenic thaumatin-like protein (Ole e 13) was confirmed, among the other 231, as the most abundant protein in the olive pulp. The present research has also been undertaken with the aim of identifying proteins in olive oil and ascertaining the relative contribution of seed and pulp proteins in their presence, if any, in oils.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22387115</PMID><DateCompleted><Year>2012</Year><Month>08</Month><Day>16</Day></DateCompleted><DateRevised><Year>2016</Year><Month>05</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1876-7737</ISSN><JournalIssue CitedMedium="Internet"><Volume>75</Volume><Issue>8</Issue><PubDate><Year>2012</Year><Month>Apr</Month><Day>18</Day></PubDate></JournalIssue><Title>Journal of proteomics</Title><ISOAbbreviation>J Proteomics</ISOAbbreviation></Journal><ArticleTitle>Identification of olive (Olea europaea) seed and pulp proteins by nLC-MS/MS via combinatorial peptide ligand libraries.</ArticleTitle><Pagination><MedlinePgn>2396-403</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jprot.2012.02.020</ELocationID><Abstract><AbstractText>Different types of extraction protocols are described for identifying proteins in seed and pulp of olive (Olea europea), by employing both conventional extraction methods and capture with ProteoMiner as well as with in house-made combinatorial peptide ligand libraries (HM-CPLLs) at pH 7.4 and at pH 2.2. Thanks to the use of CPLLs, able to dramatically amplify the signal of low-abundance species, a quite large number of compounds has been indeed identified: 61 in the seed (vs. only four reported in current literature) and 231 in the pulp (vs. 56 described so far), the deepest investigation up to the present of the olive proteome. In the seed, it highlights the presence of seed storage proteins, oleosins and histones. In the pulp, the allergenic thaumatin-like protein (Ole e 13) was confirmed, among the other 231, as the most abundant protein in the olive pulp. The present research has also been undertaken with the aim of identifying proteins in olive oil and ascertaining the relative contribution of seed and pulp proteins in their presence, if any, in oils.</AbstractText><CopyrightInformation>Copyright © 2012 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Esteve</LastName><ForeName>Clara</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Analytical Chemistry, Faculty of Chemistry, University of Alcalá, Ctra. Madrid-Barcelona, Km. 33.600, E-28871 Alcalá de Henares, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>D'Amato</LastName><ForeName>Alfonsina</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Marina</LastName><ForeName>María Luisa</ForeName><Initials>ML</Initials></Author><Author ValidYN="Y"><LastName>García</LastName><ForeName>María Concepción</ForeName><Initials>MC</Initials></Author><Author ValidYN="Y"><LastName>Citterio</LastName><ForeName>Attilio</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Righetti</LastName><ForeName>Pier Giorgio</ForeName><Initials>PG</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>02</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Proteomics</MedlineTA><NlmUniqueID>101475056</NlmUniqueID><ISSNLinking>1874-3919</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008024">Ligands</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019151">Peptide Library</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002853" MajorTopicYN="N">Chromatography, Liquid</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020650" MajorTopicYN="N">Combinatorial Chemistry Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005638" MajorTopicYN="N">Fruit</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008024" MajorTopicYN="N">Ligands</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008954" MajorTopicYN="N">Models, Biological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D031658" MajorTopicYN="N">Olea</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019151" MajorTopicYN="Y">Peptide 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PubStatus="received"><Year>2012</Year><Month>01</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>02</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>02</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>3</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>3</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>8</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22387115</ArticleId><ArticleId IdType="pii">S1874-3919(12)00104-2</ArticleId><ArticleId IdType="doi">10.1016/j.jprot.2012.02.020</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country><region><li>Communauté de Madrid</li></region><settlement><li>Madrid</li></settlement></list><tree><noCountry><name sortKey="Citterio, Attilio" sort="Citterio, Attilio" uniqKey="Citterio A" first="Attilio" last="Citterio">Attilio Citterio</name><name sortKey="D Amato, Alfonsina" sort="D Amato, Alfonsina" uniqKey="D Amato A" first="Alfonsina" last="D'Amato">Alfonsina D'Amato</name><name sortKey="Garcia, Maria Concepci N" sort="Garcia, Maria Concepci N" uniqKey="Garcia M" first="María Concepci N" last="García">María Concepci N García</name><name sortKey="Marina, Maria Luisa" sort="Marina, Maria Luisa" uniqKey="Marina M" first="María Luisa" last="Marina">María Luisa Marina</name><name sortKey="Righetti, Pier Giorgio" sort="Righetti, Pier Giorgio" uniqKey="Righetti P" first="Pier Giorgio" last="Righetti">Pier Giorgio Righetti</name></noCountry><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Esteve, Clara" sort="Esteve, Clara" uniqKey="Esteve C" first="Clara" last="Esteve">Clara Esteve</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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