Discovery of Putative Herbicide Resistance Genes and Its Regulatory Network in Chickpea Using Transcriptome Sequencing.
Identifieur interne : 000090 ( Main/Exploration ); précédent : 000089; suivant : 000091Discovery of Putative Herbicide Resistance Genes and Its Regulatory Network in Chickpea Using Transcriptome Sequencing.
Auteurs : Mir A. Iquebal [Inde] ; Khela R. Soren [Inde] ; Priyanka Gangwar [Inde] ; P S Shanmugavadivel [Inde] ; K. Aravind [Inde] ; Deepak Singla [Inde] ; Sarika Jaiswal [Inde] ; Rahul S. Jasrotia [Inde] ; Sushil K. Chaturvedi [Inde] ; Narendra P. Singh [Inde] ; Rajeev K. Varshney [Inde] ; Anil Rai [Inde] ; Dinesh Kumar [Inde]Source :
- Frontiers in plant science [ 1664-462X ] ; 2017.
Abstract
Background: Chickpea (Cicer arietinum L.) contributes 75% of total pulse production. Being cheaper than animal protein, makes it important in dietary requirement of developing countries. Weed not only competes with chickpea resulting into drastic yield reduction but also creates problem of harboring fungi, bacterial diseases and insect pests. Chemical approach having new herbicide discovery has constraint of limited lead molecule options, statutory regulations and environmental clearance. Through genetic approach, transgenic herbicide tolerant crop has given successful result but led to serious concern over ecological safety thus non-transgenic approach like marker assisted selection is desirable. Since large variability in tolerance limit of herbicide already exists in chickpea varieties, thus the genes offering herbicide tolerance can be introgressed in variety improvement programme. Transcriptome studies can discover such associated key genes with herbicide tolerance in chickpea. Results: This is first transcriptomic studies of chickpea or even any legume crop using two herbicide susceptible and tolerant genotypes exposed to imidazoline (Imazethapyr). Approximately 90 million paired-end reads generated from four samples were processed and assembled into 30,803 contigs using reference based assembly. We report 6,310 differentially expressed genes (DEGs), of which 3,037 were regulated by 980 miRNAs, 1,528 transcription factors associated with 897 DEGs, 47 Hub proteins, 3,540 putative Simple Sequence Repeat-Functional Domain Marker (SSR-FDM), 13,778 genic Single Nucleotide Polymorphism (SNP) putative markers and 1,174 Indels. Randomly selected 20 DEGs were validated using qPCR. Pathway analysis suggested that xenobiotic degradation related gene, glutathione S-transferase (GST) were only up-regulated in presence of herbicide. Down-regulation of DNA replication genes and up-regulation of abscisic acid pathway genes were observed. Study further reveals the role of cytochrome P450, xyloglucan endotransglucosylase/hydrolase, glutamate dehydrogenase, methyl crotonoyl carboxylase and of thaumatin-like genes in herbicide resistance. Conclusion: Reported DEGs can be used as genomic resource for future discovery of candidate genes associated with herbicide tolerance. Reported markers can be used for future association studies in order to develop marker assisted selection (MAS) for refinement. In endeavor of chickpea variety development programme, these findings can be of immense use in improving productivity of chickpea germplasm.
DOI: 10.3389/fpls.2017.00958
PubMed: 28638398
PubMed Central: PMC5461349
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Discovery of Putative Herbicide Resistance Genes and Its Regulatory Network in Chickpea Using Transcriptome Sequencing.</title><author><name sortKey="Iquebal, Mir A" sort="Iquebal, Mir A" uniqKey="Iquebal M" first="Mir A" last="Iquebal">Mir A. Iquebal</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:regionArea><wicri:noRegion>Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Soren, Khela R" sort="Soren, Khela R" uniqKey="Soren K" first="Khela R" last="Soren">Khela R. Soren</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur</wicri:regionArea><wicri:noRegion>Indian Institute of Pulses Research (ICAR)Kanpur</wicri:noRegion></affiliation></author><author><name sortKey="Gangwar, Priyanka" sort="Gangwar, Priyanka" uniqKey="Gangwar P" first="Priyanka" last="Gangwar">Priyanka Gangwar</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur</wicri:regionArea><wicri:noRegion>Indian Institute of Pulses Research (ICAR)Kanpur</wicri:noRegion></affiliation></author><author><name sortKey="Shanmugavadivel, P S" sort="Shanmugavadivel, P S" uniqKey="Shanmugavadivel P" first="P S" last="Shanmugavadivel">P S Shanmugavadivel</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur</wicri:regionArea><wicri:noRegion>Indian Institute of Pulses Research (ICAR)Kanpur</wicri:noRegion></affiliation></author><author><name sortKey="Aravind, K" sort="Aravind, K" uniqKey="Aravind K" first="K" last="Aravind">K. Aravind</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur</wicri:regionArea><wicri:noRegion>Indian Institute of Pulses Research (ICAR)Kanpur</wicri:noRegion></affiliation></author><author><name sortKey="Singla, Deepak" sort="Singla, Deepak" uniqKey="Singla D" first="Deepak" last="Singla">Deepak Singla</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:regionArea><wicri:noRegion>Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Jaiswal, Sarika" sort="Jaiswal, Sarika" uniqKey="Jaiswal S" first="Sarika" last="Jaiswal">Sarika Jaiswal</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:regionArea><wicri:noRegion>Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Jasrotia, Rahul S" sort="Jasrotia, Rahul S" uniqKey="Jasrotia R" first="Rahul S" last="Jasrotia">Rahul S. Jasrotia</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:regionArea><wicri:noRegion>Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Chaturvedi, Sushil K" sort="Chaturvedi, Sushil K" uniqKey="Chaturvedi S" first="Sushil K" last="Chaturvedi">Sushil K. Chaturvedi</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur</wicri:regionArea><wicri:noRegion>Indian Institute of Pulses Research (ICAR)Kanpur</wicri:noRegion></affiliation></author><author><name sortKey="Singh, Narendra P" sort="Singh, Narendra P" uniqKey="Singh N" first="Narendra P" last="Singh">Narendra P. Singh</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur</wicri:regionArea><wicri:noRegion>Indian Institute of Pulses Research (ICAR)Kanpur</wicri:noRegion></affiliation></author><author><name sortKey="Varshney, Rajeev K" sort="Varshney, Rajeev K" uniqKey="Varshney R" first="Rajeev K" last="Varshney">Rajeev K. Varshney</name><affiliation wicri:level="1"><nlm:affiliation>Genetic Gains, International Crops Research Institute for the Semi-Arid TropicsPatancheru, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Genetic Gains, International Crops Research Institute for the Semi-Arid TropicsPatancheru</wicri:regionArea><wicri:noRegion>International Crops Research Institute for the Semi-Arid TropicsPatancheru</wicri:noRegion></affiliation></author><author><name sortKey="Rai, Anil" sort="Rai, Anil" uniqKey="Rai A" first="Anil" last="Rai">Anil Rai</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:regionArea><wicri:noRegion>Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Kumar, Dinesh" sort="Kumar, Dinesh" uniqKey="Kumar D" first="Dinesh" last="Kumar">Dinesh Kumar</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:regionArea><wicri:noRegion>Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28638398</idno><idno type="pmid">28638398</idno><idno type="doi">10.3389/fpls.2017.00958</idno><idno type="pmc">PMC5461349</idno><idno type="wicri:Area/Main/Corpus">000085</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000085</idno><idno type="wicri:Area/Main/Curation">000085</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000085</idno><idno type="wicri:Area/Main/Exploration">000085</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Discovery of Putative Herbicide Resistance Genes and Its Regulatory Network in Chickpea Using Transcriptome Sequencing.</title><author><name sortKey="Iquebal, Mir A" sort="Iquebal, Mir A" uniqKey="Iquebal M" first="Mir A" last="Iquebal">Mir A. Iquebal</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:regionArea><wicri:noRegion>Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Soren, Khela R" sort="Soren, Khela R" uniqKey="Soren K" first="Khela R" last="Soren">Khela R. Soren</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur</wicri:regionArea><wicri:noRegion>Indian Institute of Pulses Research (ICAR)Kanpur</wicri:noRegion></affiliation></author><author><name sortKey="Gangwar, Priyanka" sort="Gangwar, Priyanka" uniqKey="Gangwar P" first="Priyanka" last="Gangwar">Priyanka Gangwar</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur</wicri:regionArea><wicri:noRegion>Indian Institute of Pulses Research (ICAR)Kanpur</wicri:noRegion></affiliation></author><author><name sortKey="Shanmugavadivel, P S" sort="Shanmugavadivel, P S" uniqKey="Shanmugavadivel P" first="P S" last="Shanmugavadivel">P S Shanmugavadivel</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur</wicri:regionArea><wicri:noRegion>Indian Institute of Pulses Research (ICAR)Kanpur</wicri:noRegion></affiliation></author><author><name sortKey="Aravind, K" sort="Aravind, K" uniqKey="Aravind K" first="K" last="Aravind">K. Aravind</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur</wicri:regionArea><wicri:noRegion>Indian Institute of Pulses Research (ICAR)Kanpur</wicri:noRegion></affiliation></author><author><name sortKey="Singla, Deepak" sort="Singla, Deepak" uniqKey="Singla D" first="Deepak" last="Singla">Deepak Singla</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:regionArea><wicri:noRegion>Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Jaiswal, Sarika" sort="Jaiswal, Sarika" uniqKey="Jaiswal S" first="Sarika" last="Jaiswal">Sarika Jaiswal</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:regionArea><wicri:noRegion>Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Jasrotia, Rahul S" sort="Jasrotia, Rahul S" uniqKey="Jasrotia R" first="Rahul S" last="Jasrotia">Rahul S. Jasrotia</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:regionArea><wicri:noRegion>Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Chaturvedi, Sushil K" sort="Chaturvedi, Sushil K" uniqKey="Chaturvedi S" first="Sushil K" last="Chaturvedi">Sushil K. Chaturvedi</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur</wicri:regionArea><wicri:noRegion>Indian Institute of Pulses Research (ICAR)Kanpur</wicri:noRegion></affiliation></author><author><name sortKey="Singh, Narendra P" sort="Singh, Narendra P" uniqKey="Singh N" first="Narendra P" last="Singh">Narendra P. Singh</name><affiliation wicri:level="1"><nlm:affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur</wicri:regionArea><wicri:noRegion>Indian Institute of Pulses Research (ICAR)Kanpur</wicri:noRegion></affiliation></author><author><name sortKey="Varshney, Rajeev K" sort="Varshney, Rajeev K" uniqKey="Varshney R" first="Rajeev K" last="Varshney">Rajeev K. Varshney</name><affiliation wicri:level="1"><nlm:affiliation>Genetic Gains, International Crops Research Institute for the Semi-Arid TropicsPatancheru, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Genetic Gains, International Crops Research Institute for the Semi-Arid TropicsPatancheru</wicri:regionArea><wicri:noRegion>International Crops Research Institute for the Semi-Arid TropicsPatancheru</wicri:noRegion></affiliation></author><author><name sortKey="Rai, Anil" sort="Rai, Anil" uniqKey="Rai A" first="Anil" last="Rai">Anil Rai</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:regionArea><wicri:noRegion>Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Kumar, Dinesh" sort="Kumar, Dinesh" uniqKey="Kumar D" first="Dinesh" last="Kumar">Dinesh Kumar</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:regionArea><wicri:noRegion>Indian Agricultural Statistics Research Institute (ICAR)New Delhi</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><b>Background:</b> Chickpea (<i>Cicer arietinum</i> L.) contributes 75% of total pulse production. Being cheaper than animal protein, makes it important in dietary requirement of developing countries. Weed not only competes with chickpea resulting into drastic yield reduction but also creates problem of harboring fungi, bacterial diseases and insect pests. Chemical approach having new herbicide discovery has constraint of limited lead molecule options, statutory regulations and environmental clearance. Through genetic approach, transgenic herbicide tolerant crop has given successful result but led to serious concern over ecological safety thus non-transgenic approach like marker assisted selection is desirable. Since large variability in tolerance limit of herbicide already exists in chickpea varieties, thus the genes offering herbicide tolerance can be introgressed in variety improvement programme. Transcriptome studies can discover such associated key genes with herbicide tolerance in chickpea. <b>Results:</b> This is first transcriptomic studies of chickpea or even any legume crop using two herbicide susceptible and tolerant genotypes exposed to imidazoline (Imazethapyr). Approximately 90 million paired-end reads generated from four samples were processed and assembled into 30,803 contigs using reference based assembly. We report 6,310 differentially expressed genes (DEGs), of which 3,037 were regulated by 980 miRNAs, 1,528 transcription factors associated with 897 DEGs, 47 Hub proteins, 3,540 putative Simple Sequence Repeat-Functional Domain Marker (SSR-FDM), 13,778 genic Single Nucleotide Polymorphism (SNP) putative markers and 1,174 Indels. Randomly selected 20 DEGs were validated using qPCR. Pathway analysis suggested that xenobiotic degradation related gene, glutathione S-transferase (GST) were only up-regulated in presence of herbicide. Down-regulation of DNA replication genes and up-regulation of abscisic acid pathway genes were observed. Study further reveals the role of cytochrome P450, xyloglucan endotransglucosylase/hydrolase, glutamate dehydrogenase, methyl crotonoyl carboxylase and of thaumatin-like genes in herbicide resistance. <b>Conclusion:</b> Reported DEGs can be used as genomic resource for future discovery of candidate genes associated with herbicide tolerance. Reported markers can be used for future association studies in order to develop marker assisted selection (MAS) for refinement. In endeavor of chickpea variety development programme, these findings can be of immense use in improving productivity of chickpea germplasm.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">28638398</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Discovery of Putative Herbicide Resistance Genes and Its Regulatory Network in Chickpea Using Transcriptome Sequencing.</ArticleTitle><Pagination><MedlinePgn>958</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2017.00958</ELocationID><Abstract><AbstractText><b>Background:</b> Chickpea (<i>Cicer arietinum</i> L.) contributes 75% of total pulse production. Being cheaper than animal protein, makes it important in dietary requirement of developing countries. Weed not only competes with chickpea resulting into drastic yield reduction but also creates problem of harboring fungi, bacterial diseases and insect pests. Chemical approach having new herbicide discovery has constraint of limited lead molecule options, statutory regulations and environmental clearance. Through genetic approach, transgenic herbicide tolerant crop has given successful result but led to serious concern over ecological safety thus non-transgenic approach like marker assisted selection is desirable. Since large variability in tolerance limit of herbicide already exists in chickpea varieties, thus the genes offering herbicide tolerance can be introgressed in variety improvement programme. Transcriptome studies can discover such associated key genes with herbicide tolerance in chickpea. <b>Results:</b> This is first transcriptomic studies of chickpea or even any legume crop using two herbicide susceptible and tolerant genotypes exposed to imidazoline (Imazethapyr). Approximately 90 million paired-end reads generated from four samples were processed and assembled into 30,803 contigs using reference based assembly. We report 6,310 differentially expressed genes (DEGs), of which 3,037 were regulated by 980 miRNAs, 1,528 transcription factors associated with 897 DEGs, 47 Hub proteins, 3,540 putative Simple Sequence Repeat-Functional Domain Marker (SSR-FDM), 13,778 genic Single Nucleotide Polymorphism (SNP) putative markers and 1,174 Indels. Randomly selected 20 DEGs were validated using qPCR. Pathway analysis suggested that xenobiotic degradation related gene, glutathione S-transferase (GST) were only up-regulated in presence of herbicide. Down-regulation of DNA replication genes and up-regulation of abscisic acid pathway genes were observed. Study further reveals the role of cytochrome P450, xyloglucan endotransglucosylase/hydrolase, glutamate dehydrogenase, methyl crotonoyl carboxylase and of thaumatin-like genes in herbicide resistance. <b>Conclusion:</b> Reported DEGs can be used as genomic resource for future discovery of candidate genes associated with herbicide tolerance. Reported markers can be used for future association studies in order to develop marker assisted selection (MAS) for refinement. In endeavor of chickpea variety development programme, these findings can be of immense use in improving productivity of chickpea germplasm.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Iquebal</LastName><ForeName>Mir A</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Soren</LastName><ForeName>Khela R</ForeName><Initials>KR</Initials><AffiliationInfo><Affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gangwar</LastName><ForeName>Priyanka</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shanmugavadivel</LastName><ForeName>P S</ForeName><Initials>PS</Initials><AffiliationInfo><Affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aravind</LastName><ForeName>K</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Singla</LastName><ForeName>Deepak</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jaiswal</LastName><ForeName>Sarika</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jasrotia</LastName><ForeName>Rahul S</ForeName><Initials>RS</Initials><AffiliationInfo><Affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chaturvedi</LastName><ForeName>Sushil K</ForeName><Initials>SK</Initials><AffiliationInfo><Affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Singh</LastName><ForeName>Narendra P</ForeName><Initials>NP</Initials><AffiliationInfo><Affiliation>Division of Plant Biotechnology, Indian Institute of Pulses Research (ICAR)Kanpur, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Varshney</LastName><ForeName>Rajeev K</ForeName><Initials>RK</Initials><AffiliationInfo><Affiliation>Genetic Gains, International Crops Research Institute for the Semi-Arid TropicsPatancheru, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rai</LastName><ForeName>Anil</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Dinesh</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute (ICAR)New Delhi, India.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>06</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Imazethapyr</Keyword><Keyword MajorTopicYN="N">chickpea</Keyword><Keyword MajorTopicYN="N">differentially expressed genes</Keyword><Keyword MajorTopicYN="N">gene regulatory network</Keyword><Keyword MajorTopicYN="N">herbicide</Keyword><Keyword MajorTopicYN="N">molecular markers</Keyword><Keyword MajorTopicYN="N">transcriptome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>12</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>05</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>6</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>6</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>6</Month><Day>24</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28638398</ArticleId><ArticleId IdType="doi">10.3389/fpls.2017.00958</ArticleId><ArticleId IdType="pmc">PMC5461349</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Physiol. 1991 May;96(1):310-3</Citation><ArticleIdList><ArticleId IdType="pubmed">16668171</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2015 Jan 15;31(2):166-9</Citation><ArticleIdList><ArticleId IdType="pubmed">25260700</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Genet. 2013;9(1):e1003215</Citation><ArticleIdList><ArticleId IdType="pubmed">23349638</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2007 Jan 19;352(3):733-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17141185</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2015 Aug 11;5:12806</Citation><ArticleIdList><ArticleId IdType="pubmed">26259924</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Genet Genomics. 2010 Apr;283(4):397-413</Citation><ArticleIdList><ArticleId IdType="pubmed">20213187</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2013 Mar;31(3):240-6</Citation><ArticleIdList><ArticleId IdType="pubmed">23354103</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2006 Aug;18(8):2051-65</Citation><ArticleIdList><ArticleId IdType="pubmed">16861386</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2010 Jan 1;26(1):139-40</Citation><ArticleIdList><ArticleId IdType="pubmed">19910308</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2007 May 15;23(10):1289-91</Citation><ArticleIdList><ArticleId IdType="pubmed">17379693</ArticleId></ArticleIdList></Reference><Reference><Citation>Theor Appl Genet. 2014 Jul;127(7):1583-91</Citation><ArticleIdList><ArticleId IdType="pubmed">24821525</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol Biochem. 2008 Mar;46(3):309-24</Citation><ArticleIdList><ArticleId IdType="pubmed">18234503</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2013 Apr 25;14(4):R36</Citation><ArticleIdList><ArticleId IdType="pubmed">23618408</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2013 Jan;64(2):445-58</Citation><ArticleIdList><ArticleId IdType="pubmed">23307915</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2012;7(10):e46415</Citation><ArticleIdList><ArticleId IdType="pubmed">23056304</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2003 Nov;13(11):2498-504</Citation><ArticleIdList><ArticleId IdType="pubmed">14597658</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2010 Jan;38(Database issue):D822-7</Citation><ArticleIdList><ArticleId IdType="pubmed">19858103</ArticleId></ArticleIdList></Reference><Reference><Citation>Pest Manag Sci. 2005 Mar;61(3):246-57</Citation><ArticleIdList><ArticleId IdType="pubmed">15627242</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2010 May;28(5):511-5</Citation><ArticleIdList><ArticleId IdType="pubmed">20436464</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2014 Aug 1;30(15):2114-20</Citation><ArticleIdList><ArticleId IdType="pubmed">24695404</ArticleId></ArticleIdList></Reference><Reference><Citation>Pest Manag Sci. 2005 Mar;61(3):211-8</Citation><ArticleIdList><ArticleId IdType="pubmed">15660452</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2005 Sep;167(3):787-96</Citation><ArticleIdList><ArticleId IdType="pubmed">16101915</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2013 Aug;8(8):1494-512</Citation><ArticleIdList><ArticleId IdType="pubmed">23845962</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 2004 Dec 15;52(25):7601-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15675810</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2009 Aug 15;25(16):2078-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19505943</ArticleId></ArticleIdList></Reference><Reference><Citation>Biomed Res Int. 2014;2014:629697</Citation><ArticleIdList><ArticleId IdType="pubmed">25136606</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1984 Jun 29;224(4656):1443-5</Citation><ArticleIdList><ArticleId IdType="pubmed">17793381</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2016 Oct;14 (10 ):1941-55</Citation><ArticleIdList><ArticleId IdType="pubmed">26990124</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2017 Jun;90(5):868-885</Citation><ArticleIdList><ArticleId IdType="pubmed">27859875</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Plant Biol. 2014 Dec 30;14:380</Citation><ArticleIdList><ArticleId IdType="pubmed">25547170</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2015 May 12;16:93</Citation><ArticleIdList><ArticleId IdType="pubmed">25962727</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2016 Dec 15;7:1871</Citation><ArticleIdList><ArticleId IdType="pubmed">28018404</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2011 May;30(5):867-77</Citation><ArticleIdList><ArticleId IdType="pubmed">21207033</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2011 Jul;39(Web Server issue):W155-9</Citation><ArticleIdList><ArticleId IdType="pubmed">21622958</ArticleId></ArticleIdList></Reference><Reference><Citation>J Hazard Mater. 2013 Apr 15;250-251:204-11</Citation><ArticleIdList><ArticleId IdType="pubmed">23454459</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13010-5</Citation><ArticleIdList><ArticleId IdType="pubmed">16916934</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2014 Apr;9(4):939-49</Citation><ArticleIdList><ArticleId IdType="pubmed">24675734</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 2011 Jun 8;59(11):5819-29</Citation><ArticleIdList><ArticleId IdType="pubmed">20586458</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 1990 Oct 5;215(3):403-10</Citation><ArticleIdList><ArticleId IdType="pubmed">2231712</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2009 Apr;7(3):266-82</Citation><ArticleIdList><ArticleId IdType="pubmed">19222808</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 1999 Oct;15(10):776-84</Citation><ArticleIdList><ArticleId IdType="pubmed">10705431</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Jan 17;103(3):569-73</Citation><ArticleIdList><ArticleId IdType="pubmed">16407096</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2002 May;14 (5):1109-20</Citation><ArticleIdList><ArticleId IdType="pubmed">12034900</ArticleId></ArticleIdList></Reference><Reference><Citation>G3 (Bethesda). 2014 Aug 12;4(11):2065-78</Citation><ArticleIdList><ArticleId IdType="pubmed">25122667</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2011 Jun 07;12:299</Citation><ArticleIdList><ArticleId IdType="pubmed">21649926</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2016 Jul 28;6:30412</Citation><ArticleIdList><ArticleId IdType="pubmed">27465480</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2000;132:365-86</Citation><ArticleIdList><ArticleId IdType="pubmed">10547847</ArticleId></ArticleIdList></Reference><Reference><Citation>Pest Manag Sci. 2005 Mar;61(3):312-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15668961</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2012 Aug;40(15):e115</Citation><ArticleIdList><ArticleId IdType="pubmed">22730293</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2007 Jul;35(Web Server issue):W182-5</Citation><ArticleIdList><ArticleId IdType="pubmed">17526522</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2007 Jan;12(1):6-13</Citation><ArticleIdList><ArticleId IdType="pubmed">17161644</ArticleId></ArticleIdList></Reference><Reference><Citation>Fly (Austin). 2012 Apr-Jun;6(2):80-92</Citation><ArticleIdList><ArticleId IdType="pubmed">22728672</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Mol Sci. 2013 Mar 01;14(3):4912-33</Citation><ArticleIdList><ArticleId IdType="pubmed">23455465</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Mol Sci. 2016 Dec 22;18(1):</Citation><ArticleIdList><ArticleId IdType="pubmed">28025485</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Med Genomics. 2015;8 Suppl 2:S10</Citation><ArticleIdList><ArticleId IdType="pubmed">26044212</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1987 Sep;6(9):2513-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16453789</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Plant Genomics. 2008;2008:619832</Citation><ArticleIdList><ArticleId IdType="pubmed">18483572</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Syst Biol. 2015 Aug 07;11(8):826</Citation><ArticleIdList><ArticleId IdType="pubmed">26253570</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2014 Nov 05;5:623</Citation><ArticleIdList><ArticleId IdType="pubmed">25414720</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2015 Jun 12;6:443</Citation><ArticleIdList><ArticleId IdType="pubmed">26124770</ArticleId></ArticleIdList></Reference><Reference><Citation>Database (Oxford). 2015 Aug 19;2015:null</Citation><ArticleIdList><ArticleId IdType="pubmed">26289427</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2006 Jan 1;34(Database issue):D140-4</Citation><ArticleIdList><ArticleId IdType="pubmed">16381832</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2017 Apr 18;:null</Citation><ArticleIdList><ArticleId IdType="pubmed">28418615</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Comput Biol. 2013;9(5):e1003068</Citation><ArticleIdList><ArticleId IdType="pubmed">23717196</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell Biol. 2012 Jan;32(2):385-98</Citation><ArticleIdList><ArticleId IdType="pubmed">22083952</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Genet. 2016 Feb 23;7:24</Citation><ArticleIdList><ArticleId IdType="pubmed">26941778</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Iquebal, Mir A" sort="Iquebal, Mir A" uniqKey="Iquebal M" first="Mir A" last="Iquebal">Mir A. Iquebal</name></noRegion><name sortKey="Aravind, K" sort="Aravind, K" uniqKey="Aravind K" first="K" last="Aravind">K. Aravind</name><name sortKey="Chaturvedi, Sushil K" sort="Chaturvedi, Sushil K" uniqKey="Chaturvedi S" first="Sushil K" last="Chaturvedi">Sushil K. Chaturvedi</name><name sortKey="Gangwar, Priyanka" sort="Gangwar, Priyanka" uniqKey="Gangwar P" first="Priyanka" last="Gangwar">Priyanka Gangwar</name><name sortKey="Jaiswal, Sarika" sort="Jaiswal, Sarika" uniqKey="Jaiswal S" first="Sarika" last="Jaiswal">Sarika Jaiswal</name><name sortKey="Jasrotia, Rahul S" sort="Jasrotia, Rahul S" uniqKey="Jasrotia R" first="Rahul S" last="Jasrotia">Rahul S. Jasrotia</name><name sortKey="Kumar, Dinesh" sort="Kumar, Dinesh" uniqKey="Kumar D" first="Dinesh" last="Kumar">Dinesh Kumar</name><name sortKey="Rai, Anil" sort="Rai, Anil" uniqKey="Rai A" first="Anil" last="Rai">Anil Rai</name><name sortKey="Shanmugavadivel, P S" sort="Shanmugavadivel, P S" uniqKey="Shanmugavadivel P" first="P S" last="Shanmugavadivel">P S Shanmugavadivel</name><name sortKey="Singh, Narendra P" sort="Singh, Narendra P" uniqKey="Singh N" first="Narendra P" last="Singh">Narendra P. Singh</name><name sortKey="Singla, Deepak" sort="Singla, Deepak" uniqKey="Singla D" first="Deepak" last="Singla">Deepak Singla</name><name sortKey="Soren, Khela R" sort="Soren, Khela R" uniqKey="Soren K" first="Khela R" last="Soren">Khela R. Soren</name><name sortKey="Varshney, Rajeev K" sort="Varshney, Rajeev K" uniqKey="Varshney R" first="Rajeev K" last="Varshney">Rajeev K. Varshney</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/ThaumatinV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000090 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000090 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= ThaumatinV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:28638398
|texte= Discovery of Putative Herbicide Resistance Genes and Its Regulatory Network in Chickpea Using Transcriptome Sequencing.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:28638398" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ThaumatinV1
| This area was generated with Dilib version V0.6.37. |  |