ThaumatinV1, Main, Curation, bibRecord, 000264

Osmotin purified from the latex of Calotropis procera: biochemical characterization, biological activity and role in plant defense.

Identifieur interne : 000264 ( Main/Curation ); précédent : 000263; suivant : 000265

Osmotin purified from the latex of Calotropis procera: biochemical characterization, biological activity and role in plant defense.

Auteurs : Cleverson Diniz Teixeira De Freitas [Brésil] ; Fábio César Sousa Nogueira ; Ilka Maria Vasconcelos ; José Tadeu Abreu Oliveira ; Gilberto Barbosa Domont ; Márcio Viana Ramos

Source :

Plant physiology and biochemistry : PPB [ 1873-2690 ] ; 2011.

RBID : pubmed:21334906

Descripteurs français

KwdFr :
- Antifongiques (composition chimique), Antifongiques (isolement et purification), Antifongiques (métabolisme), Antifongiques (pharmacologie), Calotropis (composition chimique), Calotropis (métabolisme), Colletotrichum (effets des médicaments et des substances chimiques), Fusarium (effets des médicaments et des substances chimiques), Glycoprotéines (composition chimique), Glycoprotéines (isolement et purification), Glycoprotéines (métabolisme), Glycoprotéines (pharmacologie), Immunité des plantes (MeSH), Isoformes de protéines (MeSH), Latex (composition chimique), Latex (métabolisme), Masse moléculaire (MeSH), Neurospora (effets des médicaments et des substances chimiques), Protéines végétales (composition chimique), Protéines végétales (isolement et purification), Protéines végétales (métabolisme), Protéines végétales (pharmacologie), Spectrométrie de masse (MeSH), Spectrométrie de masse MALDI (MeSH), Séquence d'acides aminés (MeSH), Électrophorèse bidimensionnelle sur gel (MeSH), Électrophorèse sur gel de polyacrylamide (MeSH).
MESH :
- composition chimique : Antifongiques, Calotropis, Glycoprotéines, Latex, Protéines végétales.
- effets des médicaments et des substances chimiques : Colletotrichum, Fusarium, Neurospora.
- isolement et purification : Antifongiques, Glycoprotéines, Protéines végétales.
- métabolisme : Antifongiques, Calotropis, Glycoprotéines, Latex, Protéines végétales.
- pharmacologie : Antifongiques, Glycoprotéines, Protéines végétales.
- Immunité des plantes, Isoformes de protéines, Masse moléculaire, Spectrométrie de masse, Spectrométrie de masse MALDI, Séquence d'acides aminés, Électrophorèse bidimensionnelle sur gel, Électrophorèse sur gel de polyacrylamide.

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Antifungal Agents (chemistry), Antifungal Agents (isolation & purification), Antifungal Agents (metabolism), Antifungal Agents (pharmacology), Calotropis (chemistry), Calotropis (metabolism), Colletotrichum (drug effects), Electrophoresis, Gel, Two-Dimensional (MeSH), Electrophoresis, Polyacrylamide Gel (MeSH), Fusarium (drug effects), Glycoproteins (chemistry), Glycoproteins (isolation & purification), Glycoproteins (metabolism), Glycoproteins (pharmacology), Latex (chemistry), Latex (metabolism), Mass Spectrometry (MeSH), Molecular Weight (MeSH), Neurospora (drug effects), Plant Immunity (MeSH), Plant Proteins (chemistry), Plant Proteins (isolation & purification), Plant Proteins (metabolism), Plant Proteins (pharmacology), Protein Isoforms (MeSH), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH).
MESH :
- chemical , chemistry : Antifungal Agents, Glycoproteins, Latex, Plant Proteins.
- chemical , isolation & purification : Antifungal Agents, Glycoproteins, Plant Proteins.
- chemical , metabolism : Antifungal Agents, Glycoproteins, Latex, Plant Proteins.
- chemical , pharmacology : Antifungal Agents, Glycoproteins, Plant Proteins.
- chemistry : Calotropis.
- drug effects : Colletotrichum, Fusarium, Neurospora.
- metabolism : Calotropis.
- Amino Acid Sequence, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Mass Spectrometry, Molecular Weight, Plant Immunity, Protein Isoforms, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.

Abstract

A protein, similar to osmotin- and thaumatin-like proteins, was purified from Calotropis procera (Ait.) R.Br latex. The isolation procedure required two cation exchange chromatography steps on 50mM Na-acetate buffer (pH 5.0) CM-Sepharose Fast Flow and 25 mM Na-phosphate buffer (pH 6.0) Resource-S, respectively. The protein purity was confirmed by an unique N-terminal sequence [ATFTIRNNCPYTIWAAAVPGGGRRLNSGGTWTINVAPGTA]. The osmotin (CpOsm) appeared as a single band (20,100 Da) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and as two spots in two-dimensional electrophoresis (pI 8.9 and 9.1). Both polypeptides were further identified by mass spectrometry as two osmotin isoforms with molecular masses of 22,340 and 22,536 Da. The CpOsm exerted antifungal activity against Fusarium solani (IC₅₀=67.0 μg mL⁻¹), Neurospora sp. (IC₅₀=57.5 μg mL⁻¹) and Colletotrichum gloeosporioides (IC₅₀=32.1 μg mL⁻¹). However, this activity was lost when the protein was previously treated with a reducing agent (DTT, Dithiothreitol) suggesting the presence of disulfide bounds stabilizing the protein. The occurrence of osmotin in latex substantiates the defensive role of these fluids.

DOI: 10.1016/j.plaphy.2011.01.027
PubMed: 21334906

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Le document en format XML

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<term>Antifungal Agents (isolation & purification)</term>
<term>Antifungal Agents (metabolism)</term>
<term>Antifungal Agents (pharmacology)</term>
<term>Calotropis (chemistry)</term>
<term>Calotropis (metabolism)</term>
<term>Colletotrichum (drug effects)</term>
<term>Electrophoresis, Gel, Two-Dimensional (MeSH)</term>
<term>Electrophoresis, Polyacrylamide Gel (MeSH)</term>
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<term>Glycoproteins (chemistry)</term>
<term>Glycoproteins (isolation & purification)</term>
<term>Glycoproteins (metabolism)</term>
<term>Glycoproteins (pharmacology)</term>
<term>Latex (chemistry)</term>
<term>Latex (metabolism)</term>
<term>Mass Spectrometry (MeSH)</term>
<term>Molecular Weight (MeSH)</term>
<term>Neurospora (drug effects)</term>
<term>Plant Immunity (MeSH)</term>
<term>Plant Proteins (chemistry)</term>
<term>Plant Proteins (isolation & purification)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plant Proteins (pharmacology)</term>
<term>Protein Isoforms (MeSH)</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Antifongiques (composition chimique)</term>
<term>Antifongiques (isolement et purification)</term>
<term>Antifongiques (métabolisme)</term>
<term>Antifongiques (pharmacologie)</term>
<term>Calotropis (composition chimique)</term>
<term>Calotropis (métabolisme)</term>
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<term>Glycoprotéines (composition chimique)</term>
<term>Glycoprotéines (isolement et purification)</term>
<term>Glycoprotéines (métabolisme)</term>
<term>Glycoprotéines (pharmacologie)</term>
<term>Immunité des plantes (MeSH)</term>
<term>Isoformes de protéines (MeSH)</term>
<term>Latex (composition chimique)</term>
<term>Latex (métabolisme)</term>
<term>Masse moléculaire (MeSH)</term>
<term>Neurospora (effets des médicaments et des substances chimiques)</term>
<term>Protéines végétales (composition chimique)</term>
<term>Protéines végétales (isolement et purification)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Protéines végétales (pharmacologie)</term>
<term>Spectrométrie de masse (MeSH)</term>
<term>Spectrométrie de masse MALDI (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Électrophorèse bidimensionnelle sur gel (MeSH)</term>
<term>Électrophorèse sur gel de polyacrylamide (MeSH)</term>
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<term>Glycoproteins</term>
<term>Latex</term>
<term>Plant Proteins</term>
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<term>Plant Proteins</term>
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<term>Glycoproteins</term>
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<term>Calotropis</term>
<term>Glycoprotéines</term>
<term>Latex</term>
<term>Protéines végétales</term>
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<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Colletotrichum</term>
<term>Fusarium</term>
<term>Neurospora</term>
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<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Colletotrichum</term>
<term>Fusarium</term>
<term>Neurospora</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antifongiques</term>
<term>Glycoprotéines</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Calotropis</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antifongiques</term>
<term>Calotropis</term>
<term>Glycoprotéines</term>
<term>Latex</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antifongiques</term>
<term>Glycoprotéines</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Electrophoresis, Gel, Two-Dimensional</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Mass Spectrometry</term>
<term>Molecular Weight</term>
<term>Plant Immunity</term>
<term>Protein Isoforms</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term>
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<term>Isoformes de protéines</term>
<term>Masse moléculaire</term>
<term>Spectrométrie de masse</term>
<term>Spectrométrie de masse MALDI</term>
<term>Séquence d'acides aminés</term>
<term>Électrophorèse bidimensionnelle sur gel</term>
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<front><div type="abstract" xml:lang="en">A protein, similar to osmotin- and thaumatin-like proteins, was purified from Calotropis procera (Ait.) R.Br latex. The isolation procedure required two cation exchange chromatography steps on 50mM Na-acetate buffer (pH 5.0) CM-Sepharose Fast Flow and 25 mM Na-phosphate buffer (pH 6.0) Resource-S, respectively. The protein purity was confirmed by an unique N-terminal sequence [ATFTIRNNCPYTIWAAAVPGGGRRLNSGGTWTINVAPGTA]. The osmotin (CpOsm) appeared as a single band (20,100 Da) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and as two spots in two-dimensional electrophoresis (pI 8.9 and 9.1). Both polypeptides were further identified by mass spectrometry as two osmotin isoforms with molecular masses of 22,340 and 22,536 Da. The CpOsm exerted antifungal activity against Fusarium solani (IC₅₀=67.0 μg mL⁻¹), Neurospora sp. (IC₅₀=57.5 μg mL⁻¹) and Colletotrichum gloeosporioides (IC₅₀=32.1 μg mL⁻¹). However, this activity was lost when the protein was previously treated with a reducing agent (DTT, Dithiothreitol) suggesting the presence of disulfide bounds stabilizing the protein. The occurrence of osmotin in latex substantiates the defensive role of these fluids.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21334906</PMID>
<DateCompleted><Year>2012</Year>
<Month>05</Month>
<Day>07</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>09</Month>
<Day>30</Day>
</DateRevised>
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<JournalIssue CitedMedium="Internet"><Volume>49</Volume>
<Issue>7</Issue>
<PubDate><Year>2011</Year>
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</PubDate>
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<Title>Plant physiology and biochemistry : PPB</Title>
<ISOAbbreviation>Plant Physiol Biochem</ISOAbbreviation>
</Journal>
<ArticleTitle>Osmotin purified from the latex of Calotropis procera: biochemical characterization, biological activity and role in plant defense.</ArticleTitle>
<Pagination><MedlinePgn>738-43</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1016/j.plaphy.2011.01.027</ELocationID>
<Abstract><AbstractText>A protein, similar to osmotin- and thaumatin-like proteins, was purified from Calotropis procera (Ait.) R.Br latex. The isolation procedure required two cation exchange chromatography steps on 50mM Na-acetate buffer (pH 5.0) CM-Sepharose Fast Flow and 25 mM Na-phosphate buffer (pH 6.0) Resource-S, respectively. The protein purity was confirmed by an unique N-terminal sequence [ATFTIRNNCPYTIWAAAVPGGGRRLNSGGTWTINVAPGTA]. The osmotin (CpOsm) appeared as a single band (20,100 Da) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and as two spots in two-dimensional electrophoresis (pI 8.9 and 9.1). Both polypeptides were further identified by mass spectrometry as two osmotin isoforms with molecular masses of 22,340 and 22,536 Da. The CpOsm exerted antifungal activity against Fusarium solani (IC₅₀=67.0 μg mL⁻¹), Neurospora sp. (IC₅₀=57.5 μg mL⁻¹) and Colletotrichum gloeosporioides (IC₅₀=32.1 μg mL⁻¹). However, this activity was lost when the protein was previously treated with a reducing agent (DTT, Dithiothreitol) suggesting the presence of disulfide bounds stabilizing the protein. The occurrence of osmotin in latex substantiates the defensive role of these fluids.</AbstractText>
<CopyrightInformation>Copyright © 2011 Elsevier Masson SAS. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>de Freitas</LastName>
<ForeName>Cleverson Diniz Teixeira</ForeName>
<Initials>CD</Initials>
<AffiliationInfo><Affiliation>Departamento de Bioquímica e Biologia Molecular da, Universidade Federal do Ceará, Campus do Pici, Cx., Postal 6033, Fortaleza, Ceará, CEP 60451-970, Brazil. cleversondiniz@hotmail.com</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Nogueira</LastName>
<ForeName>Fábio César Sousa</ForeName>
<Initials>FC</Initials>
</Author>
<Author ValidYN="Y"><LastName>Vasconcelos</LastName>
<ForeName>Ilka Maria</ForeName>
<Initials>IM</Initials>
</Author>
<Author ValidYN="Y"><LastName>Oliveira</LastName>
<ForeName>José Tadeu Abreu</ForeName>
<Initials>JT</Initials>
</Author>
<Author ValidYN="Y"><LastName>Domont</LastName>
<ForeName>Gilberto Barbosa</ForeName>
<Initials>GB</Initials>
</Author>
<Author ValidYN="Y"><LastName>Ramos</LastName>
<ForeName>Márcio Viana</ForeName>
<Initials>MV</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
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<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
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<ArticleDate DateType="Electronic"><Year>2011</Year>
<Month>02</Month>
<Day>04</Day>
</ArticleDate>
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<MedlineJournalInfo><Country>France</Country>
<MedlineTA>Plant Physiol Biochem</MedlineTA>
<NlmUniqueID>9882449</NlmUniqueID>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000935">Antifungal Agents</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D006023">Glycoproteins</NameOfSubstance>
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<NameOfSubstance UI="D007840">Latex</NameOfSubstance>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000935" MajorTopicYN="N">Antifungal Agents</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D031107" MajorTopicYN="N">Calotropis</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020231" MajorTopicYN="N">Colletotrichum</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015180" MajorTopicYN="N">Electrophoresis, Gel, Two-Dimensional</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005670" MajorTopicYN="N">Fusarium</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006023" MajorTopicYN="N">Glycoproteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007840" MajorTopicYN="N">Latex</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013058" MajorTopicYN="N">Mass Spectrometry</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008970" MajorTopicYN="N">Molecular Weight</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009491" MajorTopicYN="N">Neurospora</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057865" MajorTopicYN="N">Plant Immunity</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName>
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Osmotin purified from the latex of Calotropis procera: biochemical characterization, biological activity and role in plant defense.

Osmotin purified from the latex of Calotropis procera: biochemical characterization, biological activity and role in plant defense.

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