Olfactory and nasal respiratory epithelia, and foliate taste buds visualized with rapid-freeze freeze-substitution and Lowicryl K11M embedding. Ultrastructural and initial cytochemical studies.
Identifieur interne : 000543 ( Main/Corpus ); précédent : 000542; suivant : 000544Olfactory and nasal respiratory epithelia, and foliate taste buds visualized with rapid-freeze freeze-substitution and Lowicryl K11M embedding. Ultrastructural and initial cytochemical studies.
Auteurs : B P MencoSource :
- Scanning microscopy [ 0891-7035 ] ; 1989.
English descriptors
- KwdEn :
- Acrylic Resins (MeSH), Animals (MeSH), Concanavalin A (metabolism), Freezing (MeSH), Histocytochemistry (MeSH), Macaca mulatta (MeSH), Male (MeSH), Microscopy, Electron (MeSH), Microtomy (methods), Nasal Mucosa (metabolism), Nasal Mucosa (ultrastructure), Olfactory Mucosa (metabolism), Olfactory Mucosa (ultrastructure), Rats (MeSH), Rats, Inbred Strains (MeSH), Taste Buds (metabolism), Taste Buds (ultrastructure).
- MESH :
- chemical , metabolism : Concanavalin A.
- chemical : Acrylic Resins.
- metabolism : Nasal Mucosa, Olfactory Mucosa, Taste Buds.
- methods : Microtomy.
- ultrastructure : Nasal Mucosa, Olfactory Mucosa, Taste Buds.
- Animals, Freezing, Histocytochemistry, Macaca mulatta, Male, Microscopy, Electron, Rats, Rats, Inbred Strains.
Abstract
Rat olfactory and respiratory epithelia and Rhesus monkey taste buds were studied with rapid-freeze, acetone/0.1% uranyl acetate freeze-substitution and low-temperature Lowicryl K11M embedding, usually in the absence of other chemical fixation and cryoprotection procedures. Ultrastructural features of mucus, cytoplasm, including cytoskeletons, and membranes were better retained than with conventional methods. Some major examples: The mucus of the olfactory epithelium consisted of a single layer; that of the respiratory epithelium had an electron-opaque sol layer surrounding cilia and microvilli below a thin laminated electron-lucent gel layer. Taste-bud pores displayed a foam-like opaque secretory product, resembling the contents of secretory granules within Type I taste-bud cells. The electron-opacity of cytoplasmic matrices sometimes obscured features such as radial spokes of respiratory cilia. Membranes had smooth outlines; those of olfactory receptor cell cilia were more electron-opaque than those of olfactory supporting cell microvilli and respiratory cilia. Membranous monolayers of many respiratory cilia across large arrays often partially split apart, all in the same direction. The space between those monolayers contained an electron-lucent substance. Preliminary cytochemistry on olfactory and nasal respiratory epithelial samples with the lectin Concanavalin A (Con A) and antibodies against olfactory marker- and odorant-binding proteins and, in taste only, the sweet-tasting protein thaumatin, were sufficiently successful to warrant further endeavors.
PubMed: 2740865
Links to Exploration step
pubmed:2740865Le document en format XML
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Ultrastructural and initial cytochemical studies.</title><author><name sortKey="Menco, B P" sort="Menco, B P" uniqKey="Menco B" first="B P" last="Menco">B P Menco</name><affiliation><nlm:affiliation>Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208-3520.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1989">1989</date><idno type="RBID">pubmed:2740865</idno><idno type="pmid">2740865</idno><idno type="wicri:Area/Main/Corpus">000543</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000543</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Olfactory and nasal respiratory epithelia, and foliate taste buds visualized with rapid-freeze freeze-substitution and Lowicryl K11M embedding. Ultrastructural and initial cytochemical studies.</title><author><name sortKey="Menco, B P" sort="Menco, B P" uniqKey="Menco B" first="B P" last="Menco">B P Menco</name><affiliation><nlm:affiliation>Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208-3520.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Scanning microscopy</title><idno type="ISSN">0891-7035</idno><imprint><date when="1989" type="published">1989</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acrylic Resins (MeSH)</term><term>Animals (MeSH)</term><term>Concanavalin A (metabolism)</term><term>Freezing (MeSH)</term><term>Histocytochemistry (MeSH)</term><term>Macaca mulatta (MeSH)</term><term>Male (MeSH)</term><term>Microscopy, Electron (MeSH)</term><term>Microtomy (methods)</term><term>Nasal Mucosa (metabolism)</term><term>Nasal Mucosa (ultrastructure)</term><term>Olfactory Mucosa (metabolism)</term><term>Olfactory Mucosa (ultrastructure)</term><term>Rats (MeSH)</term><term>Rats, Inbred Strains (MeSH)</term><term>Taste Buds (metabolism)</term><term>Taste Buds (ultrastructure)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Concanavalin A</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Acrylic Resins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Nasal Mucosa</term><term>Olfactory Mucosa</term><term>Taste Buds</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microtomy</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Nasal Mucosa</term><term>Olfactory Mucosa</term><term>Taste Buds</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Freezing</term><term>Histocytochemistry</term><term>Macaca mulatta</term><term>Male</term><term>Microscopy, Electron</term><term>Rats</term><term>Rats, Inbred Strains</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Rat olfactory and respiratory epithelia and Rhesus monkey taste buds were studied with rapid-freeze, acetone/0.1% uranyl acetate freeze-substitution and low-temperature Lowicryl K11M embedding, usually in the absence of other chemical fixation and cryoprotection procedures. Ultrastructural features of mucus, cytoplasm, including cytoskeletons, and membranes were better retained than with conventional methods. Some major examples: The mucus of the olfactory epithelium consisted of a single layer; that of the respiratory epithelium had an electron-opaque sol layer surrounding cilia and microvilli below a thin laminated electron-lucent gel layer. Taste-bud pores displayed a foam-like opaque secretory product, resembling the contents of secretory granules within Type I taste-bud cells. The electron-opacity of cytoplasmic matrices sometimes obscured features such as radial spokes of respiratory cilia. Membranes had smooth outlines; those of olfactory receptor cell cilia were more electron-opaque than those of olfactory supporting cell microvilli and respiratory cilia. Membranous monolayers of many respiratory cilia across large arrays often partially split apart, all in the same direction. The space between those monolayers contained an electron-lucent substance. Preliminary cytochemistry on olfactory and nasal respiratory epithelial samples with the lectin Concanavalin A (Con A) and antibodies against olfactory marker- and odorant-binding proteins and, in taste only, the sweet-tasting protein thaumatin, were sufficiently successful to warrant further endeavors.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2740865</PMID><DateCompleted><Year>1989</Year><Month>07</Month><Day>28</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0891-7035</ISSN><JournalIssue CitedMedium="Print"><Volume>3</Volume><Issue>1</Issue><PubDate><Year>1989</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Scanning microscopy</Title><ISOAbbreviation>Scanning Microsc</ISOAbbreviation></Journal><ArticleTitle>Olfactory and nasal respiratory epithelia, and foliate taste buds visualized with rapid-freeze freeze-substitution and Lowicryl K11M embedding. Ultrastructural and initial cytochemical studies.</ArticleTitle><Pagination><MedlinePgn>257-72</MedlinePgn></Pagination><Abstract><AbstractText>Rat olfactory and respiratory epithelia and Rhesus monkey taste buds were studied with rapid-freeze, acetone/0.1% uranyl acetate freeze-substitution and low-temperature Lowicryl K11M embedding, usually in the absence of other chemical fixation and cryoprotection procedures. Ultrastructural features of mucus, cytoplasm, including cytoskeletons, and membranes were better retained than with conventional methods. Some major examples: The mucus of the olfactory epithelium consisted of a single layer; that of the respiratory epithelium had an electron-opaque sol layer surrounding cilia and microvilli below a thin laminated electron-lucent gel layer. Taste-bud pores displayed a foam-like opaque secretory product, resembling the contents of secretory granules within Type I taste-bud cells. The electron-opacity of cytoplasmic matrices sometimes obscured features such as radial spokes of respiratory cilia. Membranes had smooth outlines; those of olfactory receptor cell cilia were more electron-opaque than those of olfactory supporting cell microvilli and respiratory cilia. Membranous monolayers of many respiratory cilia across large arrays often partially split apart, all in the same direction. The space between those monolayers contained an electron-lucent substance. Preliminary cytochemistry on olfactory and nasal respiratory epithelial samples with the lectin Concanavalin A (Con A) and antibodies against olfactory marker- and odorant-binding proteins and, in taste only, the sweet-tasting protein thaumatin, were sufficiently successful to warrant further endeavors.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Menco</LastName><ForeName>B P</ForeName><Initials>BP</Initials><AffiliationInfo><Affiliation>Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208-3520.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Scanning Microsc</MedlineTA><NlmUniqueID>8704616</NlmUniqueID><ISSNLinking>0891-7035</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000180">Acrylic Resins</NameOfSubstance></Chemical><Chemical><RegistryNumber>11028-71-0</RegistryNumber><NameOfSubstance UI="D003208">Concanavalin A</NameOfSubstance></Chemical><Chemical><RegistryNumber>121548-34-3</RegistryNumber><NameOfSubstance UI="C050066">Lowicryl K11M</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000180" MajorTopicYN="Y">Acrylic Resins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003208" MajorTopicYN="N">Concanavalin A</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005615" MajorTopicYN="N">Freezing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006651" MajorTopicYN="N">Histocytochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008253" MajorTopicYN="N">Macaca mulatta</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008854" MajorTopicYN="N">Microscopy, Electron</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008867" MajorTopicYN="N">Microtomy</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009297" MajorTopicYN="N">Nasal Mucosa</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009831" MajorTopicYN="N">Olfactory Mucosa</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011919" MajorTopicYN="N">Rats, Inbred Strains</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013650" MajorTopicYN="N">Taste Buds</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1989</Year><Month>3</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1989</Year><Month>3</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1989</Year><Month>3</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">2740865</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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