Separating nucleation and growth in protein crystallization using dynamic light scattering.
Identifieur interne : 000491 ( Main/Corpus ); précédent : 000490; suivant : 000492Separating nucleation and growth in protein crystallization using dynamic light scattering.
Auteurs : Emmanuel Saridakis ; Karsten Dierks ; Abel Moreno ; Matthias W M. Dieckmann ; Naomi E. ChayenSource :
- Acta crystallographica. Section D, Biological crystallography [ 0907-4449 ] ; 2002.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Plant Proteins, Proteins, Sweetening Agents, Trypsin.
- methods : Crystallization.
- Animals, Light, Particle Size, Scattering, Radiation, Solutions.
Abstract
A means of controlling crystallization is to separate the phases of nucleation and growth. Methods to achieve this, other than seeding, involve lowering the supersaturation by changing the temperature or diluting drops after incubating them for a given time at nucleation conditions. However, by the time nuclei or crystals are visible under the microscope too many nuclei will have formed. Dynamic Light Scattering was applied practically, to determine the most likely time for nucleation-growth decoupling to be performed successfully. The time at which DLS showed a significant change in the size-distribution of species in solution, corresponded to that optimal time.
DOI: 10.1107/s0907444902014348
PubMed: 12351869
Links to Exploration step
pubmed:12351869Le document en format XML
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<author><name sortKey="Saridakis, Emmanuel" sort="Saridakis, Emmanuel" uniqKey="Saridakis E" first="Emmanuel" last="Saridakis">Emmanuel Saridakis</name>
<affiliation><nlm:affiliation>Biological Structure and Function Section, Division of Biomedical Sciences, Faculty of Medicine, Imperial College, London SW7 2AZ, UK.</nlm:affiliation>
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<author><name sortKey="Dierks, Karsten" sort="Dierks, Karsten" uniqKey="Dierks K" first="Karsten" last="Dierks">Karsten Dierks</name>
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<author><name sortKey="Moreno, Abel" sort="Moreno, Abel" uniqKey="Moreno A" first="Abel" last="Moreno">Abel Moreno</name>
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<author><name sortKey="Dieckmann, Matthias W M" sort="Dieckmann, Matthias W M" uniqKey="Dieckmann M" first="Matthias W M" last="Dieckmann">Matthias W M. Dieckmann</name>
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<author><name sortKey="Chayen, Naomi E" sort="Chayen, Naomi E" uniqKey="Chayen N" first="Naomi E" last="Chayen">Naomi E. Chayen</name>
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<author><name sortKey="Saridakis, Emmanuel" sort="Saridakis, Emmanuel" uniqKey="Saridakis E" first="Emmanuel" last="Saridakis">Emmanuel Saridakis</name>
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<author><name sortKey="Moreno, Abel" sort="Moreno, Abel" uniqKey="Moreno A" first="Abel" last="Moreno">Abel Moreno</name>
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<author><name sortKey="Dieckmann, Matthias W M" sort="Dieckmann, Matthias W M" uniqKey="Dieckmann M" first="Matthias W M" last="Dieckmann">Matthias W M. Dieckmann</name>
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<author><name sortKey="Chayen, Naomi E" sort="Chayen, Naomi E" uniqKey="Chayen N" first="Naomi E" last="Chayen">Naomi E. Chayen</name>
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<term>Light (MeSH)</term>
<term>Particle Size (MeSH)</term>
<term>Plant Proteins (chemistry)</term>
<term>Proteins (chemistry)</term>
<term>Scattering, Radiation (MeSH)</term>
<term>Solutions (MeSH)</term>
<term>Sweetening Agents (chemistry)</term>
<term>Trypsin (chemistry)</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term>
<term>Proteins</term>
<term>Sweetening Agents</term>
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<front><div type="abstract" xml:lang="en">A means of controlling crystallization is to separate the phases of nucleation and growth. Methods to achieve this, other than seeding, involve lowering the supersaturation by changing the temperature or diluting drops after incubating them for a given time at nucleation conditions. However, by the time nuclei or crystals are visible under the microscope too many nuclei will have formed. Dynamic Light Scattering was applied practically, to determine the most likely time for nucleation-growth decoupling to be performed successfully. The time at which DLS showed a significant change in the size-distribution of species in solution, corresponded to that optimal time.</div>
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<Title>Acta crystallographica. Section D, Biological crystallography</Title>
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<ArticleTitle>Separating nucleation and growth in protein crystallization using dynamic light scattering.</ArticleTitle>
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<Abstract><AbstractText>A means of controlling crystallization is to separate the phases of nucleation and growth. Methods to achieve this, other than seeding, involve lowering the supersaturation by changing the temperature or diluting drops after incubating them for a given time at nucleation conditions. However, by the time nuclei or crystals are visible under the microscope too many nuclei will have formed. Dynamic Light Scattering was applied practically, to determine the most likely time for nucleation-growth decoupling to be performed successfully. The time at which DLS showed a significant change in the size-distribution of species in solution, corresponded to that optimal time.</AbstractText>
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