Development of a lesion-mimic phenotype in a transgenic wheat line overexpressing genes for pathogenesis-related (PR) proteins is dependent on salicylic acid concentration.
Identifieur interne : 000464 ( Main/Corpus ); précédent : 000463; suivant : 000465Development of a lesion-mimic phenotype in a transgenic wheat line overexpressing genes for pathogenesis-related (PR) proteins is dependent on salicylic acid concentration.
Auteurs : Ajith Anand ; Eric A. Schmelz ; Subbaratnam MuthukrishnanSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2003.
English descriptors
- KwdEn :
- Chitinases (genetics), Cyclopentanes (metabolism), Gene Expression (MeSH), Genes, Plant (MeSH), Glycoside Hydrolases (genetics), Oxylipins (MeSH), Phenotype (MeSH), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Proteins (genetics), Plants, Genetically Modified (MeSH), Reactive Oxygen Species (metabolism), Salicylic Acid (metabolism), Triticum (genetics), Triticum (metabolism), Triticum (microbiology).
- MESH :
- chemical , genetics : Chitinases, Glycoside Hydrolases, Plant Proteins.
- chemical , metabolism : Cyclopentanes, Reactive Oxygen Species, Salicylic Acid.
- genetics : Plant Diseases, Triticum.
- metabolism : Triticum.
- microbiology : Plant Diseases, Triticum.
- Gene Expression, Genes, Plant, Oxylipins, Phenotype, Plants, Genetically Modified.
Abstract
In the course of coexpressing genes for pathogenesis-related (PR) proteins for a class IV chitinase and an acidic glucanase in transgenic wheat plants, we regenerated a wheat line that developed necrotic lesions containing dead cells in the T2 and subsequent generations. Lesion spots were detected at the booting stage (5- to 6-week-old plants) in lines homozygous for the transgene loci. In contrast, lesions were not observed in hemizygous transgenic lines or lines silenced for transgene expression, indicating a requirement for high levels of transgene expression for the development of the lesioned phenotype. Lesion development was associated with the accumulation of host-encoded PR proteins, e.g., chitinases, beta-1,3-glucanases, thaumatin-like protein, and production of reactive oxygen intermediates. F1 progeny of a cross between the lesion-plus transgenic line and wild-type nontransgenic plants produced progeny with a normal phenotype, while the F2 progenies segregated for the lesion phenotype. Salicylic acid (SA) levels in plants with the lesion-plus phenotype were found to be several times higher than controls and nearly double the levels in hemizygous transgenic plants that lack lesions. SA application activated lesion development in excised leaf pieces of these hemizygous transgenic plants. Similar activation of lesion development in control plants occurred only when high concentrations of SA were applied for prolonged periods. Transcripts for phenylalanine-ammonia lyase, which provides precursors of SA, were elevated in homozygous transgenic plants. Our data suggest that transgene-induced lesion-mimic phenotype correlates with enhanced SA biosynthesis.
DOI: 10.1094/MPMI.2003.16.10.916
PubMed: 14558693
Links to Exploration step
pubmed:14558693Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of a lesion-mimic phenotype in a transgenic wheat line overexpressing genes for pathogenesis-related (PR) proteins is dependent on salicylic acid concentration.</title><author><name sortKey="Anand, Ajith" sort="Anand, Ajith" uniqKey="Anand A" first="Ajith" last="Anand">Ajith Anand</name><affiliation><nlm:affiliation>Department of Biochemistry, Kansas State University, Manhattan, KS 66506, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Schmelz, Eric A" sort="Schmelz, Eric A" uniqKey="Schmelz E" first="Eric A" last="Schmelz">Eric A. Schmelz</name></author><author><name sortKey="Muthukrishnan, Subbaratnam" sort="Muthukrishnan, Subbaratnam" uniqKey="Muthukrishnan S" first="Subbaratnam" last="Muthukrishnan">Subbaratnam Muthukrishnan</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:14558693</idno><idno type="pmid">14558693</idno><idno type="doi">10.1094/MPMI.2003.16.10.916</idno><idno type="wicri:Area/Main/Corpus">000464</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000464</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Development of a lesion-mimic phenotype in a transgenic wheat line overexpressing genes for pathogenesis-related (PR) proteins is dependent on salicylic acid concentration.</title><author><name sortKey="Anand, Ajith" sort="Anand, Ajith" uniqKey="Anand A" first="Ajith" last="Anand">Ajith Anand</name><affiliation><nlm:affiliation>Department of Biochemistry, Kansas State University, Manhattan, KS 66506, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Schmelz, Eric A" sort="Schmelz, Eric A" uniqKey="Schmelz E" first="Eric A" last="Schmelz">Eric A. Schmelz</name></author><author><name sortKey="Muthukrishnan, Subbaratnam" sort="Muthukrishnan, Subbaratnam" uniqKey="Muthukrishnan S" first="Subbaratnam" last="Muthukrishnan">Subbaratnam Muthukrishnan</name></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chitinases (genetics)</term><term>Cyclopentanes (metabolism)</term><term>Gene Expression (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Glycoside Hydrolases (genetics)</term><term>Oxylipins (MeSH)</term><term>Phenotype (MeSH)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plants, Genetically Modified (MeSH)</term><term>Reactive Oxygen Species (metabolism)</term><term>Salicylic Acid (metabolism)</term><term>Triticum (genetics)</term><term>Triticum (metabolism)</term><term>Triticum (microbiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Chitinases</term><term>Glycoside Hydrolases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cyclopentanes</term><term>Reactive Oxygen Species</term><term>Salicylic Acid</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Diseases</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Triticum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression</term><term>Genes, Plant</term><term>Oxylipins</term><term>Phenotype</term><term>Plants, Genetically Modified</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the course of coexpressing genes for pathogenesis-related (PR) proteins for a class IV chitinase and an acidic glucanase in transgenic wheat plants, we regenerated a wheat line that developed necrotic lesions containing dead cells in the T2 and subsequent generations. Lesion spots were detected at the booting stage (5- to 6-week-old plants) in lines homozygous for the transgene loci. In contrast, lesions were not observed in hemizygous transgenic lines or lines silenced for transgene expression, indicating a requirement for high levels of transgene expression for the development of the lesioned phenotype. Lesion development was associated with the accumulation of host-encoded PR proteins, e.g., chitinases, beta-1,3-glucanases, thaumatin-like protein, and production of reactive oxygen intermediates. F1 progeny of a cross between the lesion-plus transgenic line and wild-type nontransgenic plants produced progeny with a normal phenotype, while the F2 progenies segregated for the lesion phenotype. Salicylic acid (SA) levels in plants with the lesion-plus phenotype were found to be several times higher than controls and nearly double the levels in hemizygous transgenic plants that lack lesions. SA application activated lesion development in excised leaf pieces of these hemizygous transgenic plants. Similar activation of lesion development in control plants occurred only when high concentrations of SA were applied for prolonged periods. Transcripts for phenylalanine-ammonia lyase, which provides precursors of SA, were elevated in homozygous transgenic plants. Our data suggest that transgene-induced lesion-mimic phenotype correlates with enhanced SA biosynthesis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14558693</PMID><DateCompleted><Year>2004</Year><Month>01</Month><Day>05</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>24</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>16</Volume><Issue>10</Issue><PubDate><Year>2003</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Development of a lesion-mimic phenotype in a transgenic wheat line overexpressing genes for pathogenesis-related (PR) proteins is dependent on salicylic acid concentration.</ArticleTitle><Pagination><MedlinePgn>916-25</MedlinePgn></Pagination><Abstract><AbstractText>In the course of coexpressing genes for pathogenesis-related (PR) proteins for a class IV chitinase and an acidic glucanase in transgenic wheat plants, we regenerated a wheat line that developed necrotic lesions containing dead cells in the T2 and subsequent generations. Lesion spots were detected at the booting stage (5- to 6-week-old plants) in lines homozygous for the transgene loci. In contrast, lesions were not observed in hemizygous transgenic lines or lines silenced for transgene expression, indicating a requirement for high levels of transgene expression for the development of the lesioned phenotype. Lesion development was associated with the accumulation of host-encoded PR proteins, e.g., chitinases, beta-1,3-glucanases, thaumatin-like protein, and production of reactive oxygen intermediates. F1 progeny of a cross between the lesion-plus transgenic line and wild-type nontransgenic plants produced progeny with a normal phenotype, while the F2 progenies segregated for the lesion phenotype. Salicylic acid (SA) levels in plants with the lesion-plus phenotype were found to be several times higher than controls and nearly double the levels in hemizygous transgenic plants that lack lesions. SA application activated lesion development in excised leaf pieces of these hemizygous transgenic plants. Similar activation of lesion development in control plants occurred only when high concentrations of SA were applied for prolonged periods. Transcripts for phenylalanine-ammonia lyase, which provides precursors of SA, were elevated in homozygous transgenic plants. Our data suggest that transgene-induced lesion-mimic phenotype correlates with enhanced SA biosynthesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Anand</LastName><ForeName>Ajith</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Kansas State University, Manhattan, KS 66506, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schmelz</LastName><ForeName>Eric A</ForeName><Initials>EA</Initials></Author><Author ValidYN="Y"><LastName>Muthukrishnan</LastName><ForeName>Subbaratnam</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003517">Cyclopentanes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D054883">Oxylipins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C053376">pathogenesis-related proteins, plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>6RI5N05OWW</RegistryNumber><NameOfSubstance UI="C011006">jasmonic acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="D006026">Glycoside Hydrolases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.14</RegistryNumber><NameOfSubstance UI="D002688">Chitinases</NameOfSubstance></Chemical><Chemical><RegistryNumber>O414PZ4LPZ</RegistryNumber><NameOfSubstance UI="D020156">Salicylic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002688" MajorTopicYN="N">Chitinases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003517" MajorTopicYN="N">Cyclopentanes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006026" MajorTopicYN="N">Glycoside 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MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020156" MajorTopicYN="N">Salicylic Acid</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014908" MajorTopicYN="N">Triticum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>10</Month><Day>16</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>1</Month><Day>6</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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