Radiation damage in room-temperature data acquisition with the PILATUS 6M pixel detector.
Identifieur interne : 000259 ( Main/Corpus ); précédent : 000258; suivant : 000260Radiation damage in room-temperature data acquisition with the PILATUS 6M pixel detector.
Auteurs : Chitra Rajendran ; Florian S N. Dworkowski ; Meitian Wang ; Clemens Schulze-BrieseSource :
- Journal of synchrotron radiation [ 1600-5775 ] ; 2011.
Abstract
The first study of room-temperature macromolecular crystallography data acquisition with a silicon pixel detector is presented, where the data are collected in continuous sample rotation mode, with millisecond read-out time and no read-out noise. Several successive datasets were collected sequentially from single test crystals of thaumatin and insulin. The dose rate ranged between ∼ 1320 Gy s(-1) and ∼ 8420 Gy s(-1) with corresponding frame rates between 1.565 Hz and 12.5 Hz. The data were analysed for global radiation damage. A previously unreported negative dose-rate effect is observed in the indicators of global radiation damage, which showed an approximately 75% decrease in D(1/2) at sixfold higher dose rate. The integrated intensity decreases in an exponential manner. Sample heating that could give rise to the enhanced radiation sensitivity at higher dose rate is investigated by collecting data between crystal temperatures of 298 K and 353 K. UV-Vis spectroscopy is used to demonstrate that disulfide radicals and trapped electrons do not accumulate at high dose rates in continuous data collection.
DOI: 10.1107/S090904951100968X
PubMed: 21525639
PubMed Central: PMC3133521
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Radiation damage in room-temperature data acquisition with the PILATUS 6M pixel detector.</title><author><name sortKey="Rajendran, Chitra" sort="Rajendran, Chitra" uniqKey="Rajendran C" first="Chitra" last="Rajendran">Chitra Rajendran</name><affiliation><nlm:affiliation>Swiss Light Source at Paul Scherrer Institute, CH-5232 Villigen, Switzerland. chitra.rajendran@psi.ch</nlm:affiliation></affiliation></author><author><name sortKey="Dworkowski, Florian S N" sort="Dworkowski, Florian S N" uniqKey="Dworkowski F" first="Florian S N" last="Dworkowski">Florian S N. Dworkowski</name></author><author><name sortKey="Wang, Meitian" sort="Wang, Meitian" uniqKey="Wang M" first="Meitian" last="Wang">Meitian Wang</name></author><author><name sortKey="Schulze Briese, Clemens" sort="Schulze Briese, Clemens" uniqKey="Schulze Briese C" first="Clemens" last="Schulze-Briese">Clemens Schulze-Briese</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21525639</idno><idno type="pmid">21525639</idno><idno type="doi">10.1107/S090904951100968X</idno><idno type="pmc">PMC3133521</idno><idno type="wicri:Area/Main/Corpus">000259</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000259</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Radiation damage in room-temperature data acquisition with the PILATUS 6M pixel detector.</title><author><name sortKey="Rajendran, Chitra" sort="Rajendran, Chitra" uniqKey="Rajendran C" first="Chitra" last="Rajendran">Chitra Rajendran</name><affiliation><nlm:affiliation>Swiss Light Source at Paul Scherrer Institute, CH-5232 Villigen, Switzerland. chitra.rajendran@psi.ch</nlm:affiliation></affiliation></author><author><name sortKey="Dworkowski, Florian S N" sort="Dworkowski, Florian S N" uniqKey="Dworkowski F" first="Florian S N" last="Dworkowski">Florian S N. Dworkowski</name></author><author><name sortKey="Wang, Meitian" sort="Wang, Meitian" uniqKey="Wang M" first="Meitian" last="Wang">Meitian Wang</name></author><author><name sortKey="Schulze Briese, Clemens" sort="Schulze Briese, Clemens" uniqKey="Schulze Briese C" first="Clemens" last="Schulze-Briese">Clemens Schulze-Briese</name></author></analytic><series><title level="j">Journal of synchrotron radiation</title><idno type="eISSN">1600-5775</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The first study of room-temperature macromolecular crystallography data acquisition with a silicon pixel detector is presented, where the data are collected in continuous sample rotation mode, with millisecond read-out time and no read-out noise. Several successive datasets were collected sequentially from single test crystals of thaumatin and insulin. The dose rate ranged between ∼ 1320 Gy s(-1) and ∼ 8420 Gy s(-1) with corresponding frame rates between 1.565 Hz and 12.5 Hz. The data were analysed for global radiation damage. A previously unreported negative dose-rate effect is observed in the indicators of global radiation damage, which showed an approximately 75% decrease in D(1/2) at sixfold higher dose rate. The integrated intensity decreases in an exponential manner. Sample heating that could give rise to the enhanced radiation sensitivity at higher dose rate is investigated by collecting data between crystal temperatures of 298 K and 353 K. UV-Vis spectroscopy is used to demonstrate that disulfide radicals and trapped electrons do not accumulate at high dose rates in continuous data collection.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">21525639</PMID><DateCompleted><Year>2011</Year><Month>08</Month><Day>16</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>06</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1600-5775</ISSN><JournalIssue CitedMedium="Internet"><Volume>18</Volume><Issue>Pt 3</Issue><PubDate><Year>2011</Year><Month>May</Month></PubDate></JournalIssue><Title>Journal of synchrotron radiation</Title><ISOAbbreviation>J Synchrotron Radiat</ISOAbbreviation></Journal><ArticleTitle>Radiation damage in room-temperature data acquisition with the PILATUS 6M pixel detector.</ArticleTitle><Pagination><MedlinePgn>318-28</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1107/S090904951100968X</ELocationID><Abstract><AbstractText>The first study of room-temperature macromolecular crystallography data acquisition with a silicon pixel detector is presented, where the data are collected in continuous sample rotation mode, with millisecond read-out time and no read-out noise. Several successive datasets were collected sequentially from single test crystals of thaumatin and insulin. The dose rate ranged between ∼ 1320 Gy s(-1) and ∼ 8420 Gy s(-1) with corresponding frame rates between 1.565 Hz and 12.5 Hz. The data were analysed for global radiation damage. A previously unreported negative dose-rate effect is observed in the indicators of global radiation damage, which showed an approximately 75% decrease in D(1/2) at sixfold higher dose rate. The integrated intensity decreases in an exponential manner. Sample heating that could give rise to the enhanced radiation sensitivity at higher dose rate is investigated by collecting data between crystal temperatures of 298 K and 353 K. UV-Vis spectroscopy is used to demonstrate that disulfide radicals and trapped electrons do not accumulate at high dose rates in continuous data collection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rajendran</LastName><ForeName>Chitra</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Swiss Light Source at Paul Scherrer Institute, CH-5232 Villigen, Switzerland. chitra.rajendran@psi.ch</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dworkowski</LastName><ForeName>Florian S N</ForeName><Initials>FS</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Meitian</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Schulze-Briese</LastName><ForeName>Clemens</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate 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