Biomarker genes highlight intraspecific and interspecific variations in the responses of Pinus taeda L. and Pinus radiata D. Don to Sirex noctilio F. acid gland secretions.
Identifieur interne : 000210 ( Main/Corpus ); précédent : 000209; suivant : 000211Biomarker genes highlight intraspecific and interspecific variations in the responses of Pinus taeda L. and Pinus radiata D. Don to Sirex noctilio F. acid gland secretions.
Auteurs : John Michael Bordeaux ; W Walter Lorenz ; Jeffrey F D. DeanSource :
- Tree physiology [ 1758-4469 ] ; 2012.
English descriptors
- KwdEn :
- Animals (MeSH), Biomarkers (analysis), Female (MeSH), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (drug effects), Genes, Plant (genetics), Genotype (MeSH), Host-Parasite Interactions (MeSH), Hymenoptera (chemistry), Hymenoptera (physiology), Molecular Sequence Annotation (MeSH), North America (MeSH), Oligonucleotide Array Sequence Analysis (MeSH), Peptides (MeSH), Phenotype (MeSH), Pinus (drug effects), Pinus (genetics), Pinus (parasitology), Pinus taeda (drug effects), Pinus taeda (genetics), Pinus taeda (parasitology), Plant Diseases (parasitology), Proteolysis (MeSH), RNA, Plant (genetics), Seedlings (drug effects), Seedlings (genetics), Seedlings (parasitology), Species Specificity (MeSH), Stress, Physiological (MeSH), Wasp Venoms (isolation & purification), Wasp Venoms (pharmacology).
- MESH :
- chemical , analysis : Biomarkers.
- chemical , genetics : RNA, Plant.
- chemical , isolation & purification : Wasp Venoms.
- chemical , pharmacology : Wasp Venoms.
- geographic : North America, Peptides.
- chemistry : Hymenoptera.
- drug effects : Gene Expression Regulation, Plant, Pinus, Pinus taeda, Seedlings.
- genetics : Genes, Plant, Pinus, Pinus taeda, Seedlings.
- parasitology : Pinus, Pinus taeda, Plant Diseases, Seedlings.
- physiology : Hymenoptera.
- Animals, Female, Gene Expression Profiling, Genotype, Host-Parasite Interactions, Molecular Sequence Annotation, Oligonucleotide Array Sequence Analysis, Phenotype, Proteolysis, Species Specificity, Stress, Physiological.
Abstract
Sirex noctilio F., a Eurasian horntail woodwasp recently introduced into North America, oviposits in pines and other conifers and in the process spreads a phytopathogenic fungus that serves as a food source for its larvae. During oviposition the woodwasp also deposits mucus produced in its acid (venom) gland that alters pine defense responses and facilitates infection by the fungus. A 26,496-feature loblolly pine cDNA microarray was used to survey gene expression of pine tissue responding to S. noctilio venom. Six genes were selected for further assessment by quantitative real-time polymerase chain reaction (qRT-PCR), including one that encoded an apparent PR-4 protein and another that encoded a thaumatin-like protein. Expression of both was strongly induced in response to venom, while expression of an apparent actin gene (ACT1) was stable in response to the venom. The pattern of gene response was similar in Pinus taeda L. and Pinus radiata D. Don, but the magnitude of response in P. radiata was significantly stronger for each of the induced genes. The magnitude of the biomarker gene response to venom also varied according to genotype within these two species. The qRT-PCR assay was used to demonstrate that the primary bioactive component in S. noctilio venom is a polypeptide.
DOI: 10.1093/treephys/tps091
PubMed: 23042767
Links to Exploration step
pubmed:23042767Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Biomarker genes highlight intraspecific and interspecific variations in the responses of Pinus taeda L. and Pinus radiata D. Don to Sirex noctilio F. acid gland secretions.</title><author><name sortKey="Bordeaux, John Michael" sort="Bordeaux, John Michael" uniqKey="Bordeaux J" first="John Michael" last="Bordeaux">John Michael Bordeaux</name><affiliation><nlm:affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Lorenz, W Walter" sort="Lorenz, W Walter" uniqKey="Lorenz W" first="W Walter" last="Lorenz">W Walter Lorenz</name></author><author><name sortKey="Dean, Jeffrey F D" sort="Dean, Jeffrey F D" uniqKey="Dean J" first="Jeffrey F D" last="Dean">Jeffrey F D. Dean</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:23042767</idno><idno type="pmid">23042767</idno><idno type="doi">10.1093/treephys/tps091</idno><idno type="wicri:Area/Main/Corpus">000210</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000210</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Biomarker genes highlight intraspecific and interspecific variations in the responses of Pinus taeda L. and Pinus radiata D. Don to Sirex noctilio F. acid gland secretions.</title><author><name sortKey="Bordeaux, John Michael" sort="Bordeaux, John Michael" uniqKey="Bordeaux J" first="John Michael" last="Bordeaux">John Michael Bordeaux</name><affiliation><nlm:affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Lorenz, W Walter" sort="Lorenz, W Walter" uniqKey="Lorenz W" first="W Walter" last="Lorenz">W Walter Lorenz</name></author><author><name sortKey="Dean, Jeffrey F D" sort="Dean, Jeffrey F D" uniqKey="Dean J" first="Jeffrey F D" last="Dean">Jeffrey F D. Dean</name></author></analytic><series><title level="j">Tree physiology</title><idno type="eISSN">1758-4469</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Biomarkers (analysis)</term><term>Female (MeSH)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Genes, Plant (genetics)</term><term>Genotype (MeSH)</term><term>Host-Parasite Interactions (MeSH)</term><term>Hymenoptera (chemistry)</term><term>Hymenoptera (physiology)</term><term>Molecular Sequence Annotation (MeSH)</term><term>North America (MeSH)</term><term>Oligonucleotide Array Sequence Analysis (MeSH)</term><term>Peptides (MeSH)</term><term>Phenotype (MeSH)</term><term>Pinus (drug effects)</term><term>Pinus (genetics)</term><term>Pinus (parasitology)</term><term>Pinus taeda (drug effects)</term><term>Pinus taeda (genetics)</term><term>Pinus taeda (parasitology)</term><term>Plant Diseases (parasitology)</term><term>Proteolysis (MeSH)</term><term>RNA, Plant (genetics)</term><term>Seedlings (drug effects)</term><term>Seedlings (genetics)</term><term>Seedlings (parasitology)</term><term>Species Specificity (MeSH)</term><term>Stress, Physiological (MeSH)</term><term>Wasp Venoms (isolation & purification)</term><term>Wasp Venoms (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Biomarkers</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Wasp Venoms</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Wasp Venoms</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>North America</term><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Hymenoptera</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Pinus</term><term>Pinus taeda</term><term>Seedlings</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genes, Plant</term><term>Pinus</term><term>Pinus taeda</term><term>Seedlings</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Pinus</term><term>Pinus taeda</term><term>Plant Diseases</term><term>Seedlings</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Hymenoptera</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Gene Expression Profiling</term><term>Genotype</term><term>Host-Parasite Interactions</term><term>Molecular Sequence Annotation</term><term>Oligonucleotide Array Sequence Analysis</term><term>Phenotype</term><term>Proteolysis</term><term>Species Specificity</term><term>Stress, Physiological</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Sirex noctilio F., a Eurasian horntail woodwasp recently introduced into North America, oviposits in pines and other conifers and in the process spreads a phytopathogenic fungus that serves as a food source for its larvae. During oviposition the woodwasp also deposits mucus produced in its acid (venom) gland that alters pine defense responses and facilitates infection by the fungus. A 26,496-feature loblolly pine cDNA microarray was used to survey gene expression of pine tissue responding to S. noctilio venom. Six genes were selected for further assessment by quantitative real-time polymerase chain reaction (qRT-PCR), including one that encoded an apparent PR-4 protein and another that encoded a thaumatin-like protein. Expression of both was strongly induced in response to venom, while expression of an apparent actin gene (ACT1) was stable in response to the venom. The pattern of gene response was similar in Pinus taeda L. and Pinus radiata D. Don, but the magnitude of response in P. radiata was significantly stronger for each of the induced genes. The magnitude of the biomarker gene response to venom also varied according to genotype within these two species. The qRT-PCR assay was used to demonstrate that the primary bioactive component in S. noctilio venom is a polypeptide.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23042767</PMID><DateCompleted><Year>2013</Year><Month>04</Month><Day>18</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1758-4469</ISSN><JournalIssue CitedMedium="Internet"><Volume>32</Volume><Issue>10</Issue><PubDate><Year>2012</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Biomarker genes highlight intraspecific and interspecific variations in the responses of Pinus taeda L. and Pinus radiata D. Don to Sirex noctilio F. acid gland secretions.</ArticleTitle><Pagination><MedlinePgn>1302-12</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tps091</ELocationID><Abstract><AbstractText>Sirex noctilio F., a Eurasian horntail woodwasp recently introduced into North America, oviposits in pines and other conifers and in the process spreads a phytopathogenic fungus that serves as a food source for its larvae. During oviposition the woodwasp also deposits mucus produced in its acid (venom) gland that alters pine defense responses and facilitates infection by the fungus. A 26,496-feature loblolly pine cDNA microarray was used to survey gene expression of pine tissue responding to S. noctilio venom. Six genes were selected for further assessment by quantitative real-time polymerase chain reaction (qRT-PCR), including one that encoded an apparent PR-4 protein and another that encoded a thaumatin-like protein. Expression of both was strongly induced in response to venom, while expression of an apparent actin gene (ACT1) was stable in response to the venom. The pattern of gene response was similar in Pinus taeda L. and Pinus radiata D. Don, but the magnitude of response in P. radiata was significantly stronger for each of the induced genes. The magnitude of the biomarker gene response to venom also varied according to genotype within these two species. The qRT-PCR assay was used to demonstrate that the primary bioactive component in S. noctilio venom is a polypeptide.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bordeaux</LastName><ForeName>John Michael</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lorenz</LastName><ForeName>W Walter</ForeName><Initials>WW</Initials></Author><Author ValidYN="Y"><LastName>Dean</LastName><ForeName>Jeffrey F D</ForeName><Initials>JF</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>10</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015415">Biomarkers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010455">Peptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014862">Wasp Venoms</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015415" MajorTopicYN="N">Biomarkers</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006790" MajorTopicYN="N">Host-Parasite Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006927" MajorTopicYN="N">Hymenoptera</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058977" MajorTopicYN="N">Molecular Sequence Annotation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009656" MajorTopicYN="N" Type="Geographic">North America</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020411" MajorTopicYN="N">Oligonucleotide Array Sequence Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010455" MajorTopicYN="N">Peptides</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D028223" MajorTopicYN="N">Pinus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D041603" MajorTopicYN="N">Pinus taeda</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059748" MajorTopicYN="N">Proteolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D036226" MajorTopicYN="N">Seedlings</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014862" MajorTopicYN="N">Wasp Venoms</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>10</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>10</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>4</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23042767</ArticleId><ArticleId IdType="pii">tps091</ArticleId><ArticleId IdType="doi">10.1093/treephys/tps091</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/ThaumatinV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000210 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000210 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= ThaumatinV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:23042767
|texte= Biomarker genes highlight intraspecific and interspecific variations in the responses of Pinus taeda L. and Pinus radiata D. Don to Sirex noctilio F. acid gland secretions.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:23042767" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a ThaumatinV1
| This area was generated with Dilib version V0.6.37. |  |