Ectopic Expression of JcWRKY Confers Enhanced Resistance in Transgenic Tobacco Against Macrophomina phaseolina.
Identifieur interne : 000070 ( Main/Corpus ); précédent : 000069; suivant : 000071Ectopic Expression of JcWRKY Confers Enhanced Resistance in Transgenic Tobacco Against Macrophomina phaseolina.
Auteurs : Parinita Agarwal ; Khantika Patel ; Pradeep K. AgarwalSource :
- DNA and cell biology [ 1557-7430 ] ; 2018.
English descriptors
- KwdEn :
- Disease Resistance (genetics), Ectopic Gene Expression (genetics), Gene Expression Regulation, Plant (genetics), Mycoses (MeSH), Plant Immunity (genetics), Plant Leaves (genetics), Plant Leaves (microbiology), Plant Proteins (genetics), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Salicylic Acid (MeSH), Tobacco (genetics), Tobacco (immunology), Tobacco (metabolism), Tobacco (microbiology), Transcription Factors (genetics), Transcription Factors (metabolism).
- MESH :
- chemical , genetics : Plant Proteins, Transcription Factors.
- genetics : Disease Resistance, Ectopic Gene Expression, Gene Expression Regulation, Plant, Plant Immunity, Plant Leaves, Plants, Genetically Modified, Tobacco.
- immunology : Tobacco.
- metabolism : Plants, Genetically Modified, Tobacco, Transcription Factors.
- microbiology : Plant Leaves, Tobacco.
- Mycoses, Salicylic Acid.
Abstract
Plants possess an innate immune system comprising of a complex network of closely regulated defense responses involving differential gene expression mediated by transcription factors (TFs). The WRKYs comprise of an important plant-specific TF family, which is involved in regulation of biotic and abiotic defenses. The overexpression of JcWRKY resulted in improved resistance in transgenic tobacco against Macrophomina phaseolina. The production of reactive oxygen species (ROS) and its detoxification through antioxidative system in the transgenics facilitates defense against Macrophomina. The enhanced catalase activity on Macrophomina infection limits the spread of infection. The transcript expression of antioxidative enzymes gene (CAT and SOD) and salicylic acid (SA) biosynthetic gene ICS1 showed upregulation during Macrophomina infection and combinatorial stress. The enhanced transcript of pathogenesis-related genes PR-1 indicates the accumulation of SA during different stresses. The PR-2 and PR-5 highlight the activation of defense responses comprising of activation of hydrolytic cleavage of glucanases and thaumatin-like proteins causing disruption of fungal cells. The ROS homeostasis in coordination with signaling molecules regulate the defense responses and inhibit fungal growth.
DOI: 10.1089/dna.2017.4057
PubMed: 29461864
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Agarwal</name><affiliation><nlm:affiliation>Plant Omics Division, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI) , Council of Scientific and Industrial Research (CSIR), Bhavnagar, Gujarat, India .</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29461864</idno><idno type="pmid">29461864</idno><idno type="doi">10.1089/dna.2017.4057</idno><idno type="wicri:Area/Main/Corpus">000070</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000070</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ectopic Expression of JcWRKY Confers Enhanced Resistance in Transgenic Tobacco Against Macrophomina phaseolina.</title><author><name sortKey="Agarwal, Parinita" sort="Agarwal, Parinita" uniqKey="Agarwal P" first="Parinita" last="Agarwal">Parinita Agarwal</name><affiliation><nlm:affiliation>Plant Omics Division, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI) , Council of Scientific and Industrial Research (CSIR), Bhavnagar, Gujarat, India .</nlm:affiliation></affiliation></author><author><name sortKey="Patel, Khantika" sort="Patel, Khantika" uniqKey="Patel K" first="Khantika" last="Patel">Khantika Patel</name><affiliation><nlm:affiliation>Plant Omics Division, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI) , Council of Scientific and Industrial Research (CSIR), Bhavnagar, Gujarat, India .</nlm:affiliation></affiliation></author><author><name sortKey="Agarwal, Pradeep K" sort="Agarwal, Pradeep K" uniqKey="Agarwal P" first="Pradeep K" last="Agarwal">Pradeep K. Agarwal</name><affiliation><nlm:affiliation>Plant Omics Division, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI) , Council of Scientific and Industrial Research (CSIR), Bhavnagar, Gujarat, India .</nlm:affiliation></affiliation></author></analytic><series><title level="j">DNA and cell biology</title><idno type="eISSN">1557-7430</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Disease Resistance (genetics)</term><term>Ectopic Gene Expression (genetics)</term><term>Gene Expression Regulation, Plant (genetics)</term><term>Mycoses (MeSH)</term><term>Plant Immunity (genetics)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Salicylic Acid (MeSH)</term><term>Tobacco (genetics)</term><term>Tobacco (immunology)</term><term>Tobacco (metabolism)</term><term>Tobacco (microbiology)</term><term>Transcription Factors (genetics)</term><term>Transcription Factors (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Disease Resistance</term><term>Ectopic Gene Expression</term><term>Gene Expression Regulation, Plant</term><term>Plant Immunity</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Tobacco</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Leaves</term><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Mycoses</term><term>Salicylic Acid</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plants possess an innate immune system comprising of a complex network of closely regulated defense responses involving differential gene expression mediated by transcription factors (TFs). The WRKYs comprise of an important plant-specific TF family, which is involved in regulation of biotic and abiotic defenses. The overexpression of JcWRKY resulted in improved resistance in transgenic tobacco against Macrophomina phaseolina. The production of reactive oxygen species (ROS) and its detoxification through antioxidative system in the transgenics facilitates defense against Macrophomina. The enhanced catalase activity on Macrophomina infection limits the spread of infection. The transcript expression of antioxidative enzymes gene (CAT and SOD) and salicylic acid (SA) biosynthetic gene ICS1 showed upregulation during Macrophomina infection and combinatorial stress. The enhanced transcript of pathogenesis-related genes PR-1 indicates the accumulation of SA during different stresses. The PR-2 and PR-5 highlight the activation of defense responses comprising of activation of hydrolytic cleavage of glucanases and thaumatin-like proteins causing disruption of fungal cells. The ROS homeostasis in coordination with signaling molecules regulate the defense responses and inhibit fungal growth.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29461864</PMID><DateCompleted><Year>2018</Year><Month>04</Month><Day>24</Day></DateCompleted><DateRevised><Year>2018</Year><Month>04</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1557-7430</ISSN><JournalIssue CitedMedium="Internet"><Volume>37</Volume><Issue>4</Issue><PubDate><Year>2018</Year><Month>Apr</Month></PubDate></JournalIssue><Title>DNA and cell biology</Title><ISOAbbreviation>DNA Cell Biol</ISOAbbreviation></Journal><ArticleTitle>Ectopic Expression of JcWRKY Confers Enhanced Resistance in Transgenic Tobacco Against Macrophomina phaseolina.</ArticleTitle><Pagination><MedlinePgn>298-307</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1089/dna.2017.4057</ELocationID><Abstract><AbstractText>Plants possess an innate immune system comprising of a complex network of closely regulated defense responses involving differential gene expression mediated by transcription factors (TFs). The WRKYs comprise of an important plant-specific TF family, which is involved in regulation of biotic and abiotic defenses. The overexpression of JcWRKY resulted in improved resistance in transgenic tobacco against Macrophomina phaseolina. The production of reactive oxygen species (ROS) and its detoxification through antioxidative system in the transgenics facilitates defense against Macrophomina. The enhanced catalase activity on Macrophomina infection limits the spread of infection. The transcript expression of antioxidative enzymes gene (CAT and SOD) and salicylic acid (SA) biosynthetic gene ICS1 showed upregulation during Macrophomina infection and combinatorial stress. The enhanced transcript of pathogenesis-related genes PR-1 indicates the accumulation of SA during different stresses. The PR-2 and PR-5 highlight the activation of defense responses comprising of activation of hydrolytic cleavage of glucanases and thaumatin-like proteins causing disruption of fungal cells. The ROS homeostasis in coordination with signaling molecules regulate the defense responses and inhibit fungal growth.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Agarwal</LastName><ForeName>Parinita</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Plant Omics Division, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI) , Council of Scientific and Industrial Research (CSIR), Bhavnagar, Gujarat, India .</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Patel</LastName><ForeName>Khantika</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Plant Omics Division, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI) , Council of Scientific and Industrial Research (CSIR), Bhavnagar, Gujarat, India .</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Agarwal</LastName><ForeName>Pradeep K</ForeName><Initials>PK</Initials><AffiliationInfo><Affiliation>Plant Omics Division, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI) , Council of Scientific and Industrial Research (CSIR), Bhavnagar, Gujarat, India .</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>02</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>DNA Cell Biol</MedlineTA><NlmUniqueID>9004522</NlmUniqueID><ISSNLinking>1044-5498</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>O414PZ4LPZ</RegistryNumber><NameOfSubstance UI="D020156">Salicylic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000066630" MajorTopicYN="N">Ectopic Gene Expression</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009181" MajorTopicYN="N">Mycoses</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D057865" MajorTopicYN="N">Plant Immunity</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020156" MajorTopicYN="N">Salicylic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">JcWRKY</Keyword><Keyword MajorTopicYN="N">Macrophomina phaseolina</Keyword><Keyword MajorTopicYN="N">antioxidative system</Keyword><Keyword MajorTopicYN="N">gene expression</Keyword><Keyword MajorTopicYN="N">reactive oxygen species</Keyword><Keyword MajorTopicYN="N">transcription factor</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>2</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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