Characterization of Three Osmotin-Like Proteins from Plumeria rubra and Prospection for Adiponectin Peptidomimetics.
Identifieur interne : 000021 ( Main/Corpus ); précédent : 000020; suivant : 000022Characterization of Three Osmotin-Like Proteins from Plumeria rubra and Prospection for Adiponectin Peptidomimetics.
Auteurs : Cleverson D T De Freitas ; Beatriz C. Nishi ; Camila T M. Do Nascimento ; Maria Z R. Silva ; Eduardo H S. Bezerra ; Bruno A M. Rocha ; Thalles B. Grangeiro ; João P B De Oliveira ; Pedro F Noronha Souza ; Márcio V. RamosSource :
- Protein and peptide letters [ 1875-5305 ] ; 2020.
Abstract
BACKGROUND
Osmotin-Like Proteins (OLPs) have been purified and characterized from different plant tissues, including latex fluids. Besides its defensive role, tobacco osmotin seems to induce adiponectin-like physiological effects, acting as an agonist. However, molecular information about this agonistic effect on adiponectin receptors has been poorly exploited and other osmotins have not been investigated yet.
OBJECTIVE AND METHODS
The present study involved the characterization of three OLPs from Plumeria rubra latex and molecular docking studies to evaluate the interaction between them and adiponectin receptors (AdipoR1 and AdipoR2).
RESULTS
P. rubra Osmotin-Like Proteins (PrOLPs) exhibited molecular masses from 21 to 25 kDa and isoelectric points ranging from 4.4 to 7.7. The proteins have 16 cysteine residues, which are involved in eight disulfide bonds, conserved in the same positions as other plant OLPs. The threedimensional (3D) models exhibited the three typical domains of OLPs, and molecular docking analysis showed that two PrOLP peptides interacted with two adiponectin receptors similarly to tobacco osmotin peptide.
CONCLUSION
As observed for tobacco osmotin, the latex osmotins of P. rubra exhibited compatible interactions with adiponectin receptors. Therefore, these plant defense proteins (without known counterparts in humans) are potential tools to study modulation of glucose metabolism in type II diabetes, where adiponectin plays a pivotal role in homeostasis.
DOI: 10.2174/0929866527666200129154357
PubMed: 31994998
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Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Do Nascimento, Camila T M" sort="Do Nascimento, Camila T M" uniqKey="Do Nascimento C" first="Camila T M" last="Do Nascimento">Camila T M. Do Nascimento</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Silva, Maria Z R" sort="Silva, Maria Z R" uniqKey="Silva M" first="Maria Z R" last="Silva">Maria Z R. Silva</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Bezerra, Eduardo H S" sort="Bezerra, Eduardo H S" uniqKey="Bezerra E" first="Eduardo H S" last="Bezerra">Eduardo H S. Bezerra</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Rocha, Bruno A M" sort="Rocha, Bruno A M" uniqKey="Rocha B" first="Bruno A M" last="Rocha">Bruno A M. Rocha</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Grangeiro, Thalles B" sort="Grangeiro, Thalles B" uniqKey="Grangeiro T" first="Thalles B" last="Grangeiro">Thalles B. Grangeiro</name><affiliation><nlm:affiliation>Departamento de Biologia, Fortaleza, Universidade Federal de Ceara, Ceara, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Oliveira, Joao P B De" sort="Oliveira, Joao P B De" uniqKey="Oliveira J" first="João P B De" last="Oliveira">João P B De Oliveira</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Souza, Pedro F Noronha" sort="Souza, Pedro F Noronha" uniqKey="Souza P" first="Pedro F Noronha" last="Souza">Pedro F Noronha Souza</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Ramos, Marcio V" sort="Ramos, Marcio V" uniqKey="Ramos M" first="Márcio V" last="Ramos">Márcio V. Ramos</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:31994998</idno><idno type="pmid">31994998</idno><idno type="doi">10.2174/0929866527666200129154357</idno><idno type="wicri:Area/Main/Corpus">000021</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000021</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Characterization of Three Osmotin-Like Proteins from Plumeria rubra and Prospection for Adiponectin Peptidomimetics.</title><author><name sortKey="Freitas, Cleverson D T De" sort="Freitas, Cleverson D T De" uniqKey="Freitas C" first="Cleverson D T De" last="Freitas">Cleverson D T De Freitas</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Nishi, Beatriz C" sort="Nishi, Beatriz C" uniqKey="Nishi B" first="Beatriz C" last="Nishi">Beatriz C. Nishi</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Do Nascimento, Camila T M" sort="Do Nascimento, Camila T M" uniqKey="Do Nascimento C" first="Camila T M" last="Do Nascimento">Camila T M. Do Nascimento</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Silva, Maria Z R" sort="Silva, Maria Z R" uniqKey="Silva M" first="Maria Z R" last="Silva">Maria Z R. Silva</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Bezerra, Eduardo H S" sort="Bezerra, Eduardo H S" uniqKey="Bezerra E" first="Eduardo H S" last="Bezerra">Eduardo H S. Bezerra</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Rocha, Bruno A M" sort="Rocha, Bruno A M" uniqKey="Rocha B" first="Bruno A M" last="Rocha">Bruno A M. Rocha</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Grangeiro, Thalles B" sort="Grangeiro, Thalles B" uniqKey="Grangeiro T" first="Thalles B" last="Grangeiro">Thalles B. Grangeiro</name><affiliation><nlm:affiliation>Departamento de Biologia, Fortaleza, Universidade Federal de Ceara, Ceara, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Oliveira, Joao P B De" sort="Oliveira, Joao P B De" uniqKey="Oliveira J" first="João P B De" last="Oliveira">João P B De Oliveira</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Souza, Pedro F Noronha" sort="Souza, Pedro F Noronha" uniqKey="Souza P" first="Pedro F Noronha" last="Souza">Pedro F Noronha Souza</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Ramos, Marcio V" sort="Ramos, Marcio V" uniqKey="Ramos M" first="Márcio V" last="Ramos">Márcio V. Ramos</name><affiliation><nlm:affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Protein and peptide letters</title><idno type="eISSN">1875-5305</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Osmotin-Like Proteins (OLPs) have been purified and characterized from different plant tissues, including latex fluids. Besides its defensive role, tobacco osmotin seems to induce adiponectin-like physiological effects, acting as an agonist. However, molecular information about this agonistic effect on adiponectin receptors has been poorly exploited and other osmotins have not been investigated yet.</p></div><div type="abstract" xml:lang="en"><p><b>OBJECTIVE AND METHODS</b></p><p>The present study involved the characterization of three OLPs from Plumeria rubra latex and molecular docking studies to evaluate the interaction between them and adiponectin receptors (AdipoR1 and AdipoR2).</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>P. rubra Osmotin-Like Proteins (PrOLPs) exhibited molecular masses from 21 to 25 kDa and isoelectric points ranging from 4.4 to 7.7. The proteins have 16 cysteine residues, which are involved in eight disulfide bonds, conserved in the same positions as other plant OLPs. The threedimensional (3D) models exhibited the three typical domains of OLPs, and molecular docking analysis showed that two PrOLP peptides interacted with two adiponectin receptors similarly to tobacco osmotin peptide.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>As observed for tobacco osmotin, the latex osmotins of P. rubra exhibited compatible interactions with adiponectin receptors. Therefore, these plant defense proteins (without known counterparts in humans) are potential tools to study modulation of glucose metabolism in type II diabetes, where adiponectin plays a pivotal role in homeostasis.</p></div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">31994998</PMID><DateRevised><Year>2020</Year><Month>08</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1875-5305</ISSN><JournalIssue CitedMedium="Internet"><Volume>27</Volume><Issue>7</Issue><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Protein and peptide letters</Title><ISOAbbreviation>Protein Pept Lett</ISOAbbreviation></Journal><ArticleTitle>Characterization of Three Osmotin-Like Proteins from Plumeria rubra and Prospection for Adiponectin Peptidomimetics.</ArticleTitle><Pagination><MedlinePgn>593-603</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.2174/0929866527666200129154357</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Osmotin-Like Proteins (OLPs) have been purified and characterized from different plant tissues, including latex fluids. Besides its defensive role, tobacco osmotin seems to induce adiponectin-like physiological effects, acting as an agonist. However, molecular information about this agonistic effect on adiponectin receptors has been poorly exploited and other osmotins have not been investigated yet.</AbstractText><AbstractText Label="OBJECTIVE AND METHODS" NlmCategory="OBJECTIVE">The present study involved the characterization of three OLPs from Plumeria rubra latex and molecular docking studies to evaluate the interaction between them and adiponectin receptors (AdipoR1 and AdipoR2).</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">P. rubra Osmotin-Like Proteins (PrOLPs) exhibited molecular masses from 21 to 25 kDa and isoelectric points ranging from 4.4 to 7.7. The proteins have 16 cysteine residues, which are involved in eight disulfide bonds, conserved in the same positions as other plant OLPs. The threedimensional (3D) models exhibited the three typical domains of OLPs, and molecular docking analysis showed that two PrOLP peptides interacted with two adiponectin receptors similarly to tobacco osmotin peptide.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">As observed for tobacco osmotin, the latex osmotins of P. rubra exhibited compatible interactions with adiponectin receptors. Therefore, these plant defense proteins (without known counterparts in humans) are potential tools to study modulation of glucose metabolism in type II diabetes, where adiponectin plays a pivotal role in homeostasis.</AbstractText><CopyrightInformation>Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Freitas</LastName><ForeName>Cleverson D T de</ForeName><Initials>CDT</Initials><AffiliationInfo><Affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nishi</LastName><ForeName>Beatriz C</ForeName><Initials>BC</Initials><AffiliationInfo><Affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>do Nascimento</LastName><ForeName>Camila T M</ForeName><Initials>CTM</Initials><AffiliationInfo><Affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Silva</LastName><ForeName>Maria Z R</ForeName><Initials>MZR</Initials><AffiliationInfo><Affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bezerra</LastName><ForeName>Eduardo H S</ForeName><Initials>EHS</Initials><AffiliationInfo><Affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rocha</LastName><ForeName>Bruno A M</ForeName><Initials>BAM</Initials><AffiliationInfo><Affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Grangeiro</LastName><ForeName>Thalles B</ForeName><Initials>TB</Initials><AffiliationInfo><Affiliation>Departamento de Biologia, Fortaleza, Universidade Federal de Ceara, Ceara, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Oliveira</LastName><ForeName>João P B de</ForeName><Initials>JPB</Initials><AffiliationInfo><Affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Souza</LastName><ForeName>Pedro F Noronha</ForeName><Initials>PFN</Initials><AffiliationInfo><Affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ramos</LastName><ForeName>Márcio V</ForeName><Initials>MV</Initials><AffiliationInfo><Affiliation>Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Centro de Ciencias, Bloco 907, Campus do Pici. Fortaleza, Ceara, CEP 60440-900, Brazil.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Protein Pept Lett</MedlineTA><NlmUniqueID>9441434</NlmUniqueID><ISSNLinking>0929-8665</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">AdipoR1</Keyword><Keyword MajorTopicYN="N">AdipoR2</Keyword><Keyword MajorTopicYN="N">latex</Keyword><Keyword MajorTopicYN="N">molecular modeling</Keyword><Keyword MajorTopicYN="N">phylogeny of proteins</Keyword><Keyword MajorTopicYN="N">thaumatin</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>06</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>11</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>12</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>1</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>1</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>1</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31994998</ArticleId><ArticleId IdType="pii">PPL-EPUB-104065</ArticleId><ArticleId IdType="doi">10.2174/0929866527666200129154357</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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