Aberrant mRNA processing of the maize Rp1-D rust resistance gene in wheat and barley.
Identifieur interne : 000159 ( Main/Corpus ); précédent : 000158; suivant : 000160Aberrant mRNA processing of the maize Rp1-D rust resistance gene in wheat and barley.
Auteurs : Michael A. Ayliffe ; Martin Steinau ; Robert F. Park ; Lee Rooke ; Maria G. Pacheco ; Scot H. Hulbert ; Harold N. Trick ; Anthony J. PryorSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2004.
English descriptors
- KwdEn :
- Carrier Proteins (genetics), Gene Transfer Techniques (MeSH), Hordeum (genetics), Intracellular Signaling Peptides and Proteins (MeSH), Phenotype (MeSH), Plant Proteins (genetics), Plants, Genetically Modified (MeSH), Promoter Regions, Genetic (MeSH), RNA, Messenger (genetics), RNA, Messenger (metabolism), Transcription, Genetic (MeSH), Transgenes (MeSH), Triticum (genetics), Zea mays (genetics).
- MESH :
- chemical , genetics : Carrier Proteins, Plant Proteins, RNA, Messenger.
- genetics : Hordeum, Triticum, Zea mays.
- chemical , metabolism : RNA, Messenger.
- Gene Transfer Techniques, Intracellular Signaling Peptides and Proteins, Phenotype, Plants, Genetically Modified, Promoter Regions, Genetic, Transcription, Genetic, Transgenes.
Abstract
The maize Rp1-D gene confers race-specific resistance against Puccinia sorghi (common leaf rust) isolates containing a corresponding avrRp1-D avirulence gene. An Rp1-D genomic clone and a similar Rp1-D transgene regulated by the maize ubiquitin promoter were transformed independently into susceptible maize lines and shown to confer Rp1-D resistance, demonstrating that this resistance can be transferred as a single gene. Transfer of these functional transgenes into wheat and barley did not result in novel resistances when these plants were challenged with isolates of wheat stem rust (P. graminis), wheat leaf rust (P. triticina), or barley leaf rust (P. hordei). Regardless of the promoter employed, low levels of gene expression were observed. When constitutive promoters were used for transgene expression, a majority of Rp1-D transcripts were truncated in the nucleotide binding site-encoding region by premature polyadenylation. This aberrant mRNA processing was unrelated to gene function because an inactive version of the gene also generated such transcripts. These data demonstrate that resistance gene transfer between species may not be limited only by divergence of signaling effector molecules and pathogen avirulence ligands, but potentially also by more fundamental gene expression and transcript processing limitations.
DOI: 10.1094/MPMI.2004.17.8.853
PubMed: 15305606
Links to Exploration step
pubmed:15305606Le document en format XML
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Pacheco</name></author><author><name sortKey="Hulbert, Scot H" sort="Hulbert, Scot H" uniqKey="Hulbert S" first="Scot H" last="Hulbert">Scot H. Hulbert</name></author><author><name sortKey="Trick, Harold N" sort="Trick, Harold N" uniqKey="Trick H" first="Harold N" last="Trick">Harold N. Trick</name></author><author><name sortKey="Pryor, Anthony J" sort="Pryor, Anthony J" uniqKey="Pryor A" first="Anthony J" last="Pryor">Anthony J. 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Ayliffe</name><affiliation><nlm:affiliation>CSIRO Plant Industry, Box 1600, Canberra, ACT, 2601, Australia. michael.ayliffe@csiro.au</nlm:affiliation></affiliation></author><author><name sortKey="Steinau, Martin" sort="Steinau, Martin" uniqKey="Steinau M" first="Martin" last="Steinau">Martin Steinau</name></author><author><name sortKey="Park, Robert F" sort="Park, Robert F" uniqKey="Park R" first="Robert F" last="Park">Robert F. Park</name></author><author><name sortKey="Rooke, Lee" sort="Rooke, Lee" uniqKey="Rooke L" first="Lee" last="Rooke">Lee Rooke</name></author><author><name sortKey="Pacheco, Maria G" sort="Pacheco, Maria G" uniqKey="Pacheco M" first="Maria G" last="Pacheco">Maria G. Pacheco</name></author><author><name sortKey="Hulbert, Scot H" sort="Hulbert, Scot H" uniqKey="Hulbert S" first="Scot H" last="Hulbert">Scot H. Hulbert</name></author><author><name sortKey="Trick, Harold N" sort="Trick, Harold N" uniqKey="Trick H" first="Harold N" last="Trick">Harold N. Trick</name></author><author><name sortKey="Pryor, Anthony J" sort="Pryor, Anthony J" uniqKey="Pryor A" first="Anthony J" last="Pryor">Anthony J. Pryor</name></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carrier Proteins (genetics)</term><term>Gene Transfer Techniques (MeSH)</term><term>Hordeum (genetics)</term><term>Intracellular Signaling Peptides and Proteins (MeSH)</term><term>Phenotype (MeSH)</term><term>Plant Proteins (genetics)</term><term>Plants, Genetically Modified (MeSH)</term><term>Promoter Regions, Genetic (MeSH)</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>Transcription, Genetic (MeSH)</term><term>Transgenes (MeSH)</term><term>Triticum (genetics)</term><term>Zea mays (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carrier Proteins</term><term>Plant Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Hordeum</term><term>Triticum</term><term>Zea mays</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Transfer Techniques</term><term>Intracellular Signaling Peptides and Proteins</term><term>Phenotype</term><term>Plants, Genetically Modified</term><term>Promoter Regions, Genetic</term><term>Transcription, Genetic</term><term>Transgenes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The maize Rp1-D gene confers race-specific resistance against Puccinia sorghi (common leaf rust) isolates containing a corresponding avrRp1-D avirulence gene. An Rp1-D genomic clone and a similar Rp1-D transgene regulated by the maize ubiquitin promoter were transformed independently into susceptible maize lines and shown to confer Rp1-D resistance, demonstrating that this resistance can be transferred as a single gene. Transfer of these functional transgenes into wheat and barley did not result in novel resistances when these plants were challenged with isolates of wheat stem rust (P. graminis), wheat leaf rust (P. triticina), or barley leaf rust (P. hordei). Regardless of the promoter employed, low levels of gene expression were observed. When constitutive promoters were used for transgene expression, a majority of Rp1-D transcripts were truncated in the nucleotide binding site-encoding region by premature polyadenylation. This aberrant mRNA processing was unrelated to gene function because an inactive version of the gene also generated such transcripts. These data demonstrate that resistance gene transfer between species may not be limited only by divergence of signaling effector molecules and pathogen avirulence ligands, but potentially also by more fundamental gene expression and transcript processing limitations.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15305606</PMID><DateCompleted><Year>2004</Year><Month>11</Month><Day>04</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>17</Volume><Issue>8</Issue><PubDate><Year>2004</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Aberrant mRNA processing of the maize Rp1-D rust resistance gene in wheat and barley.</ArticleTitle><Pagination><MedlinePgn>853-64</MedlinePgn></Pagination><Abstract><AbstractText>The maize Rp1-D gene confers race-specific resistance against Puccinia sorghi (common leaf rust) isolates containing a corresponding avrRp1-D avirulence gene. An Rp1-D genomic clone and a similar Rp1-D transgene regulated by the maize ubiquitin promoter were transformed independently into susceptible maize lines and shown to confer Rp1-D resistance, demonstrating that this resistance can be transferred as a single gene. Transfer of these functional transgenes into wheat and barley did not result in novel resistances when these plants were challenged with isolates of wheat stem rust (P. graminis), wheat leaf rust (P. triticina), or barley leaf rust (P. hordei). Regardless of the promoter employed, low levels of gene expression were observed. When constitutive promoters were used for transgene expression, a majority of Rp1-D transcripts were truncated in the nucleotide binding site-encoding region by premature polyadenylation. This aberrant mRNA processing was unrelated to gene function because an inactive version of the gene also generated such transcripts. These data demonstrate that resistance gene transfer between species may not be limited only by divergence of signaling effector molecules and pathogen avirulence ligands, but potentially also by more fundamental gene expression and transcript processing limitations.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ayliffe</LastName><ForeName>Michael A</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>CSIRO Plant Industry, Box 1600, Canberra, ACT, 2601, Australia. michael.ayliffe@csiro.au</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Steinau</LastName><ForeName>Martin</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Robert F</ForeName><Initials>RF</Initials></Author><Author ValidYN="Y"><LastName>Rooke</LastName><ForeName>Lee</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Pacheco</LastName><ForeName>Maria G</ForeName><Initials>MG</Initials></Author><Author ValidYN="Y"><LastName>Hulbert</LastName><ForeName>Scot H</ForeName><Initials>SH</Initials></Author><Author ValidYN="Y"><LastName>Trick</LastName><ForeName>Harold N</ForeName><Initials>HN</Initials></Author><Author ValidYN="Y"><LastName>Pryor</LastName><ForeName>Anthony J</ForeName><Initials>AJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002352">Carrier 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UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D047908" MajorTopicYN="N">Intracellular Signaling Peptides and Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="N">Transgenes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014908" MajorTopicYN="N">Triticum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003313" MajorTopicYN="N">Zea mays</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>8</Month><Day>13</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>11</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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