An improved immunolabeling method for microtubular cytoskeleton in poplar (Populus nigra L.) free nuclear endosperm.
Identifieur interne : 004B01 ( Main/Exploration ); précédent : 004B00; suivant : 004B02An improved immunolabeling method for microtubular cytoskeleton in poplar (Populus nigra L.) free nuclear endosperm.
Auteurs : X. Xu [France] ; A. Souvré ; C. Granier ; M. PetitprezSource :
- Biotechnic & histochemistry : official publication of the Biological Stain Commission [ 1052-0295 ] ; 1995.
Descripteurs français
- KwdFr :
- Arbres (MeSH), Cytosquelette (composition chimique), Facteurs temps (MeSH), Fixateurs (MeSH), Fixation tissulaire (méthodes), Formaldéhyde (MeSH), Glutaraldéhyde (MeSH), Immunohistochimie (MeSH), Inclusion de tissu (méthodes), Microtubules (composition chimique), Noyau de la cellule (composition chimique), Polymères (MeSH), Tubuline (analyse), Éthanol (MeSH).
- MESH :
- analyse : Tubuline.
- composition chimique : Cytosquelette, Microtubules, Noyau de la cellule.
- méthodes : Fixation tissulaire, Inclusion de tissu.
- Arbres, Facteurs temps, Fixateurs, Formaldéhyde, Glutaraldéhyde, Immunohistochimie, Polymères, Éthanol.
English descriptors
- KwdEn :
- Cell Nucleus (chemistry), Cytoskeleton (chemistry), Ethanol (MeSH), Fixatives (MeSH), Formaldehyde (MeSH), Glutaral (MeSH), Immunohistochemistry (MeSH), Microtubules (chemistry), Polymers (MeSH), Time Factors (MeSH), Tissue Embedding (methods), Tissue Fixation (methods), Trees (MeSH), Tubulin (analysis).
- MESH :
- chemical , analysis : Tubulin.
- chemical : Ethanol, Fixatives, Formaldehyde, Glutaral, Polymers.
- chemistry : Cell Nucleus, Cytoskeleton, Microtubules.
- methods : Tissue Embedding, Tissue Fixation.
- Immunohistochemistry, Time Factors, Trees.
Abstract
We investigated immunocytochemical staining of microtubular cytoskeleton of free nuclear endosperm, a tissue which is particularly difficult to fix. This tissue requires fixation for 45 hr to preserve the integrity of the microtubular network after paraformaldehyde based fixation. Low glutaraldehyde concentration in the fixative and the ethanol dehydration retains beta-tubulin antigenicity and the former improves preservation of tissue structure. An ethanol-free embedding method is recommended for immunocytochemical studies of ethanol sensitive target proteins.
DOI: 10.3109/10520299509108202
PubMed: 8580209
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Souvré</name></author><author><name sortKey="Granier, C" sort="Granier, C" uniqKey="Granier C" first="C" last="Granier">C. Granier</name></author><author><name sortKey="Petitprez, M" sort="Petitprez, M" uniqKey="Petitprez M" first="M" last="Petitprez">M. 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Petitprez</name></author></analytic><series><title level="j">Biotechnic & histochemistry : official publication of the Biological Stain Commission</title><idno type="ISSN">1052-0295</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Nucleus (chemistry)</term><term>Cytoskeleton (chemistry)</term><term>Ethanol (MeSH)</term><term>Fixatives (MeSH)</term><term>Formaldehyde (MeSH)</term><term>Glutaral (MeSH)</term><term>Immunohistochemistry (MeSH)</term><term>Microtubules (chemistry)</term><term>Polymers (MeSH)</term><term>Time Factors (MeSH)</term><term>Tissue Embedding (methods)</term><term>Tissue Fixation (methods)</term><term>Trees (MeSH)</term><term>Tubulin (analysis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (MeSH)</term><term>Cytosquelette (composition chimique)</term><term>Facteurs temps (MeSH)</term><term>Fixateurs (MeSH)</term><term>Fixation tissulaire (méthodes)</term><term>Formaldéhyde (MeSH)</term><term>Glutaraldéhyde (MeSH)</term><term>Immunohistochimie (MeSH)</term><term>Inclusion de tissu (méthodes)</term><term>Microtubules (composition chimique)</term><term>Noyau de la cellule (composition chimique)</term><term>Polymères (MeSH)</term><term>Tubuline (analyse)</term><term>Éthanol (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Tubulin</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Ethanol</term><term>Fixatives</term><term>Formaldehyde</term><term>Glutaral</term><term>Polymers</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Tubuline</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Nucleus</term><term>Cytoskeleton</term><term>Microtubules</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cytosquelette</term><term>Microtubules</term><term>Noyau de la cellule</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Tissue Embedding</term><term>Tissue Fixation</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Fixation tissulaire</term><term>Inclusion de tissu</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Immunohistochemistry</term><term>Time Factors</term><term>Trees</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Arbres</term><term>Facteurs temps</term><term>Fixateurs</term><term>Formaldéhyde</term><term>Glutaraldéhyde</term><term>Immunohistochimie</term><term>Polymères</term><term>Éthanol</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We investigated immunocytochemical staining of microtubular cytoskeleton of free nuclear endosperm, a tissue which is particularly difficult to fix. This tissue requires fixation for 45 hr to preserve the integrity of the microtubular network after paraformaldehyde based fixation. Low glutaraldehyde concentration in the fixative and the ethanol dehydration retains beta-tubulin antigenicity and the former improves preservation of tissue structure. An ethanol-free embedding method is recommended for immunocytochemical studies of ethanol sensitive target proteins.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8580209</PMID><DateCompleted><Year>1996</Year><Month>03</Month><Day>19</Day></DateCompleted><DateRevised><Year>2019</Year><Month>11</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1052-0295</ISSN><JournalIssue CitedMedium="Print"><Volume>70</Volume><Issue>5</Issue><PubDate><Year>1995</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Biotechnic & histochemistry : official publication of the Biological Stain Commission</Title><ISOAbbreviation>Biotech Histochem</ISOAbbreviation></Journal><ArticleTitle>An improved immunolabeling method for microtubular cytoskeleton in poplar (Populus nigra L.) free nuclear endosperm.</ArticleTitle><Pagination><MedlinePgn>252-7</MedlinePgn></Pagination><Abstract><AbstractText>We investigated immunocytochemical staining of microtubular cytoskeleton of free nuclear endosperm, a tissue which is particularly difficult to fix. This tissue requires fixation for 45 hr to preserve the integrity of the microtubular network after paraformaldehyde based fixation. Low glutaraldehyde concentration in the fixative and the ethanol dehydration retains beta-tubulin antigenicity and the former improves preservation of tissue structure. 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Granier</name><name sortKey="Petitprez, M" sort="Petitprez, M" uniqKey="Petitprez M" first="M" last="Petitprez">M. Petitprez</name><name sortKey="Souvre, A" sort="Souvre, A" uniqKey="Souvre A" first="A" last="Souvré">A. Souvré</name></noCountry><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Xu, X" sort="Xu, X" uniqKey="Xu X" first="X" last="Xu">X. Xu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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