PoplarV1, Main, Corpus, bibRecord, 004A74

An improved immunolabeling method for microtubular cytoskeleton in poplar (Populus nigra L.) free nuclear endosperm.

Identifieur interne : 004A74 ( Main/Corpus ); précédent : 004A73; suivant : 004A75

An improved immunolabeling method for microtubular cytoskeleton in poplar (Populus nigra L.) free nuclear endosperm.

Auteurs : X. Xu ; A. Souvré ; C. Granier ; M. Petitprez

Source :

Biotechnic & histochemistry : official publication of the Biological Stain Commission [ 1052-0295 ] ; 1995.

RBID : pubmed:8580209

English descriptors

KwdEn :
- Cell Nucleus (chemistry), Cytoskeleton (chemistry), Ethanol (MeSH), Fixatives (MeSH), Formaldehyde (MeSH), Glutaral (MeSH), Immunohistochemistry (MeSH), Microtubules (chemistry), Polymers (MeSH), Time Factors (MeSH), Tissue Embedding (methods), Tissue Fixation (methods), Trees (MeSH), Tubulin (analysis).
MESH :
- chemical , analysis : Tubulin.
- chemical : Ethanol, Fixatives, Formaldehyde, Glutaral, Polymers.
- chemistry : Cell Nucleus, Cytoskeleton, Microtubules.
- methods : Tissue Embedding, Tissue Fixation.
- Immunohistochemistry, Time Factors, Trees.

Abstract

We investigated immunocytochemical staining of microtubular cytoskeleton of free nuclear endosperm, a tissue which is particularly difficult to fix. This tissue requires fixation for 45 hr to preserve the integrity of the microtubular network after paraformaldehyde based fixation. Low glutaraldehyde concentration in the fixative and the ethanol dehydration retains beta-tubulin antigenicity and the former improves preservation of tissue structure. An ethanol-free embedding method is recommended for immunocytochemical studies of ethanol sensitive target proteins.

DOI: 10.3109/10520299509108202
PubMed: 8580209

Links to Exploration step

pubmed:8580209

Le document en format XML

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<author><name sortKey="Xu, X" sort="Xu, X" uniqKey="Xu X" first="X" last="Xu">X. Xu</name>
<affiliation><nlm:affiliation>Laboratory of Plant Breeding and Biotechnology, UA INPT/INRA, INP-ENSAT, Toulouse, France.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Souvre, A" sort="Souvre, A" uniqKey="Souvre A" first="A" last="Souvré">A. Souvré</name>
</author>
<author><name sortKey="Granier, C" sort="Granier, C" uniqKey="Granier C" first="C" last="Granier">C. Granier</name>
</author>
<author><name sortKey="Petitprez, M" sort="Petitprez, M" uniqKey="Petitprez M" first="M" last="Petitprez">M. Petitprez</name>
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<author><name sortKey="Xu, X" sort="Xu, X" uniqKey="Xu X" first="X" last="Xu">X. Xu</name>
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<author><name sortKey="Souvre, A" sort="Souvre, A" uniqKey="Souvre A" first="A" last="Souvré">A. Souvré</name>
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<author><name sortKey="Granier, C" sort="Granier, C" uniqKey="Granier C" first="C" last="Granier">C. Granier</name>
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<author><name sortKey="Petitprez, M" sort="Petitprez, M" uniqKey="Petitprez M" first="M" last="Petitprez">M. Petitprez</name>
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<series><title level="j">Biotechnic & histochemistry : official publication of the Biological Stain Commission</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Nucleus (chemistry)</term>
<term>Cytoskeleton (chemistry)</term>
<term>Ethanol (MeSH)</term>
<term>Fixatives (MeSH)</term>
<term>Formaldehyde (MeSH)</term>
<term>Glutaral (MeSH)</term>
<term>Immunohistochemistry (MeSH)</term>
<term>Microtubules (chemistry)</term>
<term>Polymers (MeSH)</term>
<term>Time Factors (MeSH)</term>
<term>Tissue Embedding (methods)</term>
<term>Tissue Fixation (methods)</term>
<term>Trees (MeSH)</term>
<term>Tubulin (analysis)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Tubulin</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Ethanol</term>
<term>Fixatives</term>
<term>Formaldehyde</term>
<term>Glutaral</term>
<term>Polymers</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Nucleus</term>
<term>Cytoskeleton</term>
<term>Microtubules</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Tissue Embedding</term>
<term>Tissue Fixation</term>
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<front><div type="abstract" xml:lang="en">We investigated immunocytochemical staining of microtubular cytoskeleton of free nuclear endosperm, a tissue which is particularly difficult to fix. This tissue requires fixation for 45 hr to preserve the integrity of the microtubular network after paraformaldehyde based fixation. Low glutaraldehyde concentration in the fixative and the ethanol dehydration retains beta-tubulin antigenicity and the former improves preservation of tissue structure. An ethanol-free embedding method is recommended for immunocytochemical studies of ethanol sensitive target proteins.</div>
</front>
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<DateCompleted><Year>1996</Year>
<Month>03</Month>
<Day>19</Day>
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<DateRevised><Year>2019</Year>
<Month>11</Month>
<Day>01</Day>
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<Article PubModel="Print"><Journal><ISSN IssnType="Print">1052-0295</ISSN>
<JournalIssue CitedMedium="Print"><Volume>70</Volume>
<Issue>5</Issue>
<PubDate><Year>1995</Year>
<Month>Sep</Month>
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<Title>Biotechnic & histochemistry : official publication of the Biological Stain Commission</Title>
<ISOAbbreviation>Biotech Histochem</ISOAbbreviation>
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<ArticleTitle>An improved immunolabeling method for microtubular cytoskeleton in poplar (Populus nigra L.) free nuclear endosperm.</ArticleTitle>
<Pagination><MedlinePgn>252-7</MedlinePgn>
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<Abstract><AbstractText>We investigated immunocytochemical staining of microtubular cytoskeleton of free nuclear endosperm, a tissue which is particularly difficult to fix. This tissue requires fixation for 45 hr to preserve the integrity of the microtubular network after paraformaldehyde based fixation. Low glutaraldehyde concentration in the fixative and the ethanol dehydration retains beta-tubulin antigenicity and the former improves preservation of tissue structure. An ethanol-free embedding method is recommended for immunocytochemical studies of ethanol sensitive target proteins.</AbstractText>
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<Author ValidYN="Y"><LastName>Souvré</LastName>
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<Author ValidYN="Y"><LastName>Granier</LastName>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D002467" MajorTopicYN="N">Cell Nucleus</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003599" MajorTopicYN="N">Cytoskeleton</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
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<MeshHeading><DescriptorName UI="D000431" MajorTopicYN="N">Ethanol</DescriptorName>
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<MeshHeading><DescriptorName UI="D005404" MajorTopicYN="N">Fixatives</DescriptorName>
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<MeshHeading><DescriptorName UI="D011108" MajorTopicYN="N">Polymers</DescriptorName>
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<MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016610" MajorTopicYN="N">Tissue Embedding</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016707" MajorTopicYN="N">Tissue Fixation</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014197" MajorTopicYN="Y">Trees</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014404" MajorTopicYN="N">Tubulin</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName>
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An improved immunolabeling method for microtubular cytoskeleton in poplar (Populus nigra L.) free nuclear endosperm.

An improved immunolabeling method for microtubular cytoskeleton in poplar (Populus nigra L.) free nuclear endosperm.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki