Evidence that endo-1,4-beta-glucanases act on cellulose in suspension-cultured poplar cells.
Identifieur interne : 004810 ( Main/Exploration ); précédent : 004809; suivant : 004811Evidence that endo-1,4-beta-glucanases act on cellulose in suspension-cultured poplar cells.
Auteurs : Y. Ohmiya [Japon] ; M. Samejima ; M. Shiroishi ; Y. Amano ; T. Kanda ; F. Sakai ; T. HayashiSource :
- The Plant journal : for cell and molecular biology [ 0960-7412 ] ; 2000.
Descripteurs français
- KwdFr :
- ADN complémentaire (MeSH), Acide 2,4-dichlorophénoxy-acétique (pharmacologie), Acides indolacétiques (pharmacologie), Arbres (cytologie), Arbres (enzymologie), Cellulase (génétique), Cellulase (métabolisme), Cellules cultivées (MeSH), Cellulose (métabolisme), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Glucanes (MeSH), Induction enzymatique (MeSH), Magnoliopsida (cytologie), Magnoliopsida (enzymologie), Oligosaccharides (métabolisme), Paroi cellulaire (métabolisme), Polyosides (métabolisme), Similitude de séquences d'acides aminés (MeSH), Solubilité (MeSH), Structure secondaire des protéines (MeSH), Séquence d'acides aminés (MeSH), Xylanes (MeSH).
- MESH :
- cytologie : Arbres, Magnoliopsida.
- enzymologie : Arbres, Magnoliopsida.
- génétique : Cellulase.
- métabolisme : Cellulase, Cellulose, Oligosaccharides, Paroi cellulaire, Polyosides.
- pharmacologie : Acide 2,4-dichlorophénoxy-acétique, Acides indolacétiques.
- ADN complémentaire, Cellules cultivées, Clonage moléculaire, Données de séquences moléculaires, Glucanes, Induction enzymatique, Similitude de séquences d'acides aminés, Solubilité, Structure secondaire des protéines, Séquence d'acides aminés, Xylanes.
English descriptors
- KwdEn :
- 2,4-Dichlorophenoxyacetic Acid (pharmacology), Amino Acid Sequence (MeSH), Cell Wall (metabolism), Cells, Cultured (MeSH), Cellulase (genetics), Cellulase (metabolism), Cellulose (metabolism), Cloning, Molecular (MeSH), DNA, Complementary (MeSH), Enzyme Induction (MeSH), Glucans (MeSH), Indoleacetic Acids (pharmacology), Magnoliopsida (cytology), Magnoliopsida (enzymology), Molecular Sequence Data (MeSH), Oligosaccharides (metabolism), Polysaccharides (metabolism), Protein Structure, Secondary (MeSH), Sequence Homology, Amino Acid (MeSH), Solubility (MeSH), Trees (cytology), Trees (enzymology), Xylans (MeSH).
- MESH :
- chemical , genetics : Cellulase.
- chemical , metabolism : Cellulase, Cellulose, Oligosaccharides, Polysaccharides.
- chemical , pharmacology : 2,4-Dichlorophenoxyacetic Acid, Indoleacetic Acids.
- cytology : Magnoliopsida, Trees.
- enzymology : Magnoliopsida, Trees.
- metabolism : Cell Wall.
- Amino Acid Sequence, Cells, Cultured, Cloning, Molecular, DNA, Complementary, Enzyme Induction, Glucans, Molecular Sequence Data, Protein Structure, Secondary, Sequence Homology, Amino Acid, Solubility, Xylans.
Abstract
Suspension-cultured poplar (Populus alba) cells produce two distinct endo-1,4-beta-glucanases, one of which is released in the extracellular culture medium and the other localized in their walls. Two cDNA clones, PopCel1 and PopCel2, isolated from a poplar cDNA library, encode the extracellular and the wall-bound endo-1, 4-beta-glucanases, respectively, based upon deduced amino acid sequences. The products of these two genes contained domains conserved in endo-1,4-beta-glucanase (family 9) and showed 91.5% amino acid identity. The levels of both PopCel1 and PopCel2 mRNAs increased during the lag phase of growth and decreased rapidly during the linear phase. After the levels had decreased, they were again increased by addition of sucrose to the culture medium and further enhanced by the addition of 2,4-dichlorophenoxyacetic acid (2,4-D) in the presence of sucrose. The accumulation of the mRNAs was correlated with the solubilization of cello-oligosaccharides. Cello-oligosaccharides and xyloglucan were also solubilized from the wall preparations of poplar cells incubated with enzyme preparations from the extracellular culture medium and walls. An antibody against both PopCel proteins reduced the production of cello-oligosaccharides by the extracellular enzyme by 90% and that by the wall-bound enzyme by 55%, and also prevented xyloglucan solubilization. The results show that the accumulation of poplar endo-1,4-beta-glucanases is regulated indirectly by auxin in the presence of sucrose and can act on cellulose in suspension-cultured poplar cells.
DOI: 10.1046/j.1365-313x.2000.00860.x
PubMed: 11069690
Affiliations:
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Ohmiya</name><affiliation wicri:level="4"><nlm:affiliation>Wood Research Institute, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Wood Research Institute, Kyoto University, Gokasho, Uji, Kyoto 611-0011</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Samejima, M" sort="Samejima, M" uniqKey="Samejima M" first="M" last="Samejima">M. Samejima</name></author><author><name sortKey="Shiroishi, M" sort="Shiroishi, M" uniqKey="Shiroishi M" first="M" last="Shiroishi">M. Shiroishi</name></author><author><name sortKey="Amano, Y" sort="Amano, Y" uniqKey="Amano Y" first="Y" last="Amano">Y. Amano</name></author><author><name sortKey="Kanda, T" sort="Kanda, T" uniqKey="Kanda T" first="T" last="Kanda">T. 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Hayashi</name></author></analytic><series><title level="j">The Plant journal : for cell and molecular biology</title><idno type="ISSN">0960-7412</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>2,4-Dichlorophenoxyacetic Acid (pharmacology)</term><term>Amino Acid Sequence (MeSH)</term><term>Cell Wall (metabolism)</term><term>Cells, Cultured (MeSH)</term><term>Cellulase (genetics)</term><term>Cellulase (metabolism)</term><term>Cellulose (metabolism)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Complementary (MeSH)</term><term>Enzyme Induction (MeSH)</term><term>Glucans (MeSH)</term><term>Indoleacetic Acids (pharmacology)</term><term>Magnoliopsida (cytology)</term><term>Magnoliopsida (enzymology)</term><term>Molecular Sequence Data (MeSH)</term><term>Oligosaccharides (metabolism)</term><term>Polysaccharides (metabolism)</term><term>Protein Structure, Secondary (MeSH)</term><term>Sequence Homology, Amino Acid (MeSH)</term><term>Solubility (MeSH)</term><term>Trees (cytology)</term><term>Trees (enzymology)</term><term>Xylans (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (MeSH)</term><term>Acide 2,4-dichlorophénoxy-acétique (pharmacologie)</term><term>Acides indolacétiques (pharmacologie)</term><term>Arbres (cytologie)</term><term>Arbres (enzymologie)</term><term>Cellulase (génétique)</term><term>Cellulase (métabolisme)</term><term>Cellules cultivées (MeSH)</term><term>Cellulose (métabolisme)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Glucanes (MeSH)</term><term>Induction enzymatique (MeSH)</term><term>Magnoliopsida (cytologie)</term><term>Magnoliopsida (enzymologie)</term><term>Oligosaccharides (métabolisme)</term><term>Paroi cellulaire (métabolisme)</term><term>Polyosides (métabolisme)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Solubilité (MeSH)</term><term>Structure secondaire des protéines (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Xylanes (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cellulase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulase</term><term>Cellulose</term><term>Oligosaccharides</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>2,4-Dichlorophenoxyacetic Acid</term><term>Indoleacetic Acids</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Arbres</term><term>Magnoliopsida</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Magnoliopsida</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Arbres</term><term>Magnoliopsida</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Magnoliopsida</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cellulase</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulase</term><term>Cellulose</term><term>Oligosaccharides</term><term>Paroi cellulaire</term><term>Polyosides</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acide 2,4-dichlorophénoxy-acétique</term><term>Acides indolacétiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Cells, Cultured</term><term>Cloning, Molecular</term><term>DNA, Complementary</term><term>Enzyme Induction</term><term>Glucans</term><term>Molecular Sequence Data</term><term>Protein Structure, Secondary</term><term>Sequence Homology, Amino Acid</term><term>Solubility</term><term>Xylans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN complémentaire</term><term>Cellules cultivées</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Glucanes</term><term>Induction enzymatique</term><term>Similitude de séquences d'acides aminés</term><term>Solubilité</term><term>Structure secondaire des protéines</term><term>Séquence d'acides aminés</term><term>Xylanes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Suspension-cultured poplar (Populus alba) cells produce two distinct endo-1,4-beta-glucanases, one of which is released in the extracellular culture medium and the other localized in their walls. Two cDNA clones, PopCel1 and PopCel2, isolated from a poplar cDNA library, encode the extracellular and the wall-bound endo-1, 4-beta-glucanases, respectively, based upon deduced amino acid sequences. The products of these two genes contained domains conserved in endo-1,4-beta-glucanase (family 9) and showed 91.5% amino acid identity. The levels of both PopCel1 and PopCel2 mRNAs increased during the lag phase of growth and decreased rapidly during the linear phase. After the levels had decreased, they were again increased by addition of sucrose to the culture medium and further enhanced by the addition of 2,4-dichlorophenoxyacetic acid (2,4-D) in the presence of sucrose. The accumulation of the mRNAs was correlated with the solubilization of cello-oligosaccharides. Cello-oligosaccharides and xyloglucan were also solubilized from the wall preparations of poplar cells incubated with enzyme preparations from the extracellular culture medium and walls. An antibody against both PopCel proteins reduced the production of cello-oligosaccharides by the extracellular enzyme by 90% and that by the wall-bound enzyme by 55%, and also prevented xyloglucan solubilization. The results show that the accumulation of poplar endo-1,4-beta-glucanases is regulated indirectly by auxin in the presence of sucrose and can act on cellulose in suspension-cultured poplar cells.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11069690</PMID><DateCompleted><Year>2001</Year><Month>01</Month><Day>05</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>06</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0960-7412</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>2</Issue><PubDate><Year>2000</Year><Month>Oct</Month></PubDate></JournalIssue><Title>The Plant journal : for cell and molecular biology</Title><ISOAbbreviation>Plant J</ISOAbbreviation></Journal><ArticleTitle>Evidence that endo-1,4-beta-glucanases act on cellulose in suspension-cultured poplar cells.</ArticleTitle><Pagination><MedlinePgn>147-58</MedlinePgn></Pagination><Abstract><AbstractText>Suspension-cultured poplar (Populus alba) cells produce two distinct endo-1,4-beta-glucanases, one of which is released in the extracellular culture medium and the other localized in their walls. Two cDNA clones, PopCel1 and PopCel2, isolated from a poplar cDNA library, encode the extracellular and the wall-bound endo-1, 4-beta-glucanases, respectively, based upon deduced amino acid sequences. The products of these two genes contained domains conserved in endo-1,4-beta-glucanase (family 9) and showed 91.5% amino acid identity. The levels of both PopCel1 and PopCel2 mRNAs increased during the lag phase of growth and decreased rapidly during the linear phase. After the levels had decreased, they were again increased by addition of sucrose to the culture medium and further enhanced by the addition of 2,4-dichlorophenoxyacetic acid (2,4-D) in the presence of sucrose. The accumulation of the mRNAs was correlated with the solubilization of cello-oligosaccharides. Cello-oligosaccharides and xyloglucan were also solubilized from the wall preparations of poplar cells incubated with enzyme preparations from the extracellular culture medium and walls. An antibody against both PopCel proteins reduced the production of cello-oligosaccharides by the extracellular enzyme by 90% and that by the wall-bound enzyme by 55%, and also prevented xyloglucan solubilization. The results show that the accumulation of poplar endo-1,4-beta-glucanases is regulated indirectly by auxin in the presence of sucrose and can act on cellulose in suspension-cultured poplar cells.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ohmiya</LastName><ForeName>Y</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Wood Research Institute, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Samejima</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Shiroishi</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Amano</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Kanda</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Sakai</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Hayashi</LastName><ForeName>T</ForeName><Initials>T</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>AB025795</AccessionNumber><AccessionNumber>AB025796</AccessionNumber><AccessionNumber>D32166</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant J</MedlineTA><NlmUniqueID>9207397</NlmUniqueID><ISSNLinking>0960-7412</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005936">Glucans</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007210">Indoleacetic Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009844">Oligosaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014990">Xylans</NameOfSubstance></Chemical><Chemical><RegistryNumber>2577AQ9262</RegistryNumber><NameOfSubstance UI="D015084">2,4-Dichlorophenoxyacetic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>37294-28-3</RegistryNumber><NameOfSubstance UI="C029353">xyloglucan</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>Plant J 2001 Feb;25(4):474</RefSource></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D015084" MajorTopicYN="N">2,4-Dichlorophenoxyacetic Acid</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004790" MajorTopicYN="N">Enzyme Induction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005936" MajorTopicYN="Y">Glucans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007210" MajorTopicYN="N">Indoleacetic Acids</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019684" MajorTopicYN="N">Magnoliopsida</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009844" MajorTopicYN="N">Oligosaccharides</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011134" MajorTopicYN="N">Polysaccharides</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017433" MajorTopicYN="N">Protein Structure, Secondary</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012995" MajorTopicYN="N">Solubility</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014990" MajorTopicYN="Y">Xylans</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>11</Month><Day>9</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>2</Month><Day>28</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>11</Month><Day>9</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11069690</ArticleId><ArticleId IdType="pii">tpj860</ArticleId><ArticleId IdType="doi">10.1046/j.1365-313x.2000.00860.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><noCountry><name sortKey="Amano, Y" sort="Amano, Y" uniqKey="Amano Y" first="Y" last="Amano">Y. Amano</name><name sortKey="Hayashi, T" sort="Hayashi, T" uniqKey="Hayashi T" first="T" last="Hayashi">T. Hayashi</name><name sortKey="Kanda, T" sort="Kanda, T" uniqKey="Kanda T" first="T" last="Kanda">T. Kanda</name><name sortKey="Sakai, F" sort="Sakai, F" uniqKey="Sakai F" first="F" last="Sakai">F. Sakai</name><name sortKey="Samejima, M" sort="Samejima, M" uniqKey="Samejima M" first="M" last="Samejima">M. Samejima</name><name sortKey="Shiroishi, M" sort="Shiroishi, M" uniqKey="Shiroishi M" first="M" last="Shiroishi">M. Shiroishi</name></noCountry><country name="Japon"><region name="Région du Kansai"><name sortKey="Ohmiya, Y" sort="Ohmiya, Y" uniqKey="Ohmiya Y" first="Y" last="Ohmiya">Y. Ohmiya</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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