Perturbation of wood cellulose synthesis causes pleiotropic effects in transgenic aspen.
Identifieur interne : 002D63 ( Main/Exploration ); précédent : 002D62; suivant : 002D64Perturbation of wood cellulose synthesis causes pleiotropic effects in transgenic aspen.
Auteurs : Chandrashekhar P. Joshi [États-Unis] ; Shivegowda Thammannagowda ; Takeshi Fujino ; Ji-Qing Gou ; Utku Avci ; Candace H. Haigler ; Lisa M. Mcdonnell ; Shawn D. Mansfield ; Bemnet Mengesha ; Nicholas C. Carpita ; Darby Harris ; Seth Debolt ; Gary F. PeterSource :
- Molecular plant [ 1752-9867 ] ; 2011.
Descripteurs français
- KwdFr :
- Cellulose (métabolisme), Glucosyltransferases (génétique), Glucosyltransferases (métabolisme), Lignine (métabolisme), Populus (croissance et développement), Populus (génétique), Populus (métabolisme), Végétaux génétiquement modifiés (croissance et développement), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- croissance et développement : Populus, Végétaux génétiquement modifiés.
- génétique : Glucosyltransferases, Populus, Végétaux génétiquement modifiés.
- métabolisme : Cellulose, Glucosyltransferases, Lignine, Populus, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Cellulose (metabolism), Glucosyltransferases (genetics), Glucosyltransferases (metabolism), Lignin (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (metabolism), Populus (genetics), Populus (growth & development), Populus (metabolism).
- MESH :
- chemical , genetics : Glucosyltransferases.
- chemical , metabolism : Cellulose, Glucosyltransferases, Lignin.
- genetics : Plants, Genetically Modified, Populus.
- growth & development : Plants, Genetically Modified, Populus.
- metabolism : Plants, Genetically Modified, Populus.
Abstract
Genetic manipulation of cellulose biosynthesis in trees may provide novel insights into the growth and development of trees. To explore this possibility, the overexpression of an aspen secondary wall-associated cellulose synthase (PtdCesA8) gene was attempted in transgenic aspen (Populus tremuloides L.) and unexpectedly resulted in silencing of the transgene as well as its endogenous counterparts. The main axis of the transgenic aspen plants quickly stopped growing, and weak branches adopted a weeping growth habit. Furthermore, transgenic plants initially developed smaller leaves and a less extensive root system. Secondary xylem (wood) of transgenic aspen plants contained as little as 10% cellulose normalized to dry weight compared to 41% cellulose typically found in normal aspen wood. This massive reduction in cellulose was accompanied by proportional increases in lignin (35%) and non-cellulosic polysaccharides (55%) compared to the 22% lignin and 36% non-cellulosic polysaccharides in control plants. The transgenic stems produced typical collapsed or 'irregular' xylem vessels that had altered secondary wall morphology and contained greatly reduced amounts of crystalline cellulose. These results demonstrate the fundamental role of secondary wall cellulose within the secondary xylem in maintaining the strength and structural integrity required to establish the vertical growth habit in trees.
DOI: 10.1093/mp/ssq081
PubMed: 21300756
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (growth & development)</term>
<term>Plants, Genetically Modified (metabolism)</term>
<term>Populus (genetics)</term>
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<term>Lignine (métabolisme)</term>
<term>Populus (croissance et développement)</term>
<term>Populus (génétique)</term>
<term>Populus (métabolisme)</term>
<term>Végétaux génétiquement modifiés (croissance et développement)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
<term>Végétaux génétiquement modifiés (métabolisme)</term>
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<front><div type="abstract" xml:lang="en">Genetic manipulation of cellulose biosynthesis in trees may provide novel insights into the growth and development of trees. To explore this possibility, the overexpression of an aspen secondary wall-associated cellulose synthase (PtdCesA8) gene was attempted in transgenic aspen (Populus tremuloides L.) and unexpectedly resulted in silencing of the transgene as well as its endogenous counterparts. The main axis of the transgenic aspen plants quickly stopped growing, and weak branches adopted a weeping growth habit. Furthermore, transgenic plants initially developed smaller leaves and a less extensive root system. Secondary xylem (wood) of transgenic aspen plants contained as little as 10% cellulose normalized to dry weight compared to 41% cellulose typically found in normal aspen wood. This massive reduction in cellulose was accompanied by proportional increases in lignin (35%) and non-cellulosic polysaccharides (55%) compared to the 22% lignin and 36% non-cellulosic polysaccharides in control plants. The transgenic stems produced typical collapsed or 'irregular' xylem vessels that had altered secondary wall morphology and contained greatly reduced amounts of crystalline cellulose. These results demonstrate the fundamental role of secondary wall cellulose within the secondary xylem in maintaining the strength and structural integrity required to establish the vertical growth habit in trees.</div>
</front>
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<ArticleTitle>Perturbation of wood cellulose synthesis causes pleiotropic effects in transgenic aspen.</ArticleTitle>
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<Abstract><AbstractText>Genetic manipulation of cellulose biosynthesis in trees may provide novel insights into the growth and development of trees. To explore this possibility, the overexpression of an aspen secondary wall-associated cellulose synthase (PtdCesA8) gene was attempted in transgenic aspen (Populus tremuloides L.) and unexpectedly resulted in silencing of the transgene as well as its endogenous counterparts. The main axis of the transgenic aspen plants quickly stopped growing, and weak branches adopted a weeping growth habit. Furthermore, transgenic plants initially developed smaller leaves and a less extensive root system. Secondary xylem (wood) of transgenic aspen plants contained as little as 10% cellulose normalized to dry weight compared to 41% cellulose typically found in normal aspen wood. This massive reduction in cellulose was accompanied by proportional increases in lignin (35%) and non-cellulosic polysaccharides (55%) compared to the 22% lignin and 36% non-cellulosic polysaccharides in control plants. The transgenic stems produced typical collapsed or 'irregular' xylem vessels that had altered secondary wall morphology and contained greatly reduced amounts of crystalline cellulose. These results demonstrate the fundamental role of secondary wall cellulose within the secondary xylem in maintaining the strength and structural integrity required to establish the vertical growth habit in trees.</AbstractText>
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