IS292: a novel insertion element from Agrobacterium.
Identifieur interne : 004A90 ( Main/Curation ); précédent : 004A89; suivant : 004A91IS292: a novel insertion element from Agrobacterium.
Auteurs : C. Ponsonnet [France] ; P. Normand ; G. Pilate ; X. NesmeSource :
- Microbiology (Reading, England) [ 1350-0872 ] ; 1995.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), Amorces ADN (génétique), Cartographie de restriction (MeSH), Données de séquences moléculaires (MeSH), Gènes bactériens (MeSH), Protéines bactériennes (génétique), Rhizobium (génétique), Similitude de séquences d'acides aminés (MeSH), Spécificité d'espèce (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Éléments transposables d'ADN (MeSH).
- MESH :
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Bacterial Proteins (genetics), Base Sequence (MeSH), DNA Primers (genetics), DNA Transposable Elements (MeSH), DNA, Bacterial (genetics), Genes, Bacterial (MeSH), Molecular Sequence Data (MeSH), Restriction Mapping (MeSH), Rhizobium (genetics), Sequence Homology, Amino Acid (MeSH), Species Specificity (MeSH).
- MESH :
- chemical , genetics : Bacterial Proteins, DNA Primers, DNA, Bacterial.
- genetics : Rhizobium.
- Amino Acid Sequence, Base Sequence, DNA Transposable Elements, Genes, Bacterial, Molecular Sequence Data, Restriction Mapping, Sequence Homology, Amino Acid, Species Specificity.
Abstract
A new insertion sequence, IS292, located in the 6b gene of a nopaline-type Agrobacterium strain (X88-292) isolated from poplar was identified and sequenced. IS292 is 2494 bp long, has 21 bp inverted terminal repeats with two mismatches, and generates 10 bp direct repeats upon integration. No sequence similarity was found between IS292 and other insertion elements associated with Agrobacterium, but it shows strong similarity with ISR/1 from Rhizobium leguminosarum bv. viciae. The occurrence of IS292-like sequences in various Agrobacterium isolates, especially different Agrobacterium strains isolated from the same biotope, was demonstrated by DNA hybridization.
DOI: 10.1099/13500872-141-4-853
PubMed: 7773388
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pubmed:7773388Le document en format XML
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<author><name sortKey="Ponsonnet, C" sort="Ponsonnet, C" uniqKey="Ponsonnet C" first="C" last="Ponsonnet">C. Ponsonnet</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratoire d'Ecologie Microbienne du Sol, Université Claude Bernard Lyon 1, Villeurbanne, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire d'Ecologie Microbienne du Sol, Université Claude Bernard Lyon 1, Villeurbanne</wicri:regionArea>
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<author><name sortKey="Normand, P" sort="Normand, P" uniqKey="Normand P" first="P" last="Normand">P. Normand</name>
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<author><name sortKey="Pilate, G" sort="Pilate, G" uniqKey="Pilate G" first="G" last="Pilate">G. Pilate</name>
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<term>Bacterial Proteins (genetics)</term>
<term>Base Sequence (MeSH)</term>
<term>DNA Primers (genetics)</term>
<term>DNA Transposable Elements (MeSH)</term>
<term>DNA, Bacterial (genetics)</term>
<term>Genes, Bacterial (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Restriction Mapping (MeSH)</term>
<term>Rhizobium (genetics)</term>
<term>Sequence Homology, Amino Acid (MeSH)</term>
<term>Species Specificity (MeSH)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (génétique)</term>
<term>Amorces ADN (génétique)</term>
<term>Cartographie de restriction (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Gènes bactériens (MeSH)</term>
<term>Protéines bactériennes (génétique)</term>
<term>Rhizobium (génétique)</term>
<term>Similitude de séquences d'acides aminés (MeSH)</term>
<term>Spécificité d'espèce (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>Éléments transposables d'ADN (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term>
<term>DNA Primers</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Rhizobium</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term>
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<term>Protéines bactériennes</term>
<term>Rhizobium</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>DNA Transposable Elements</term>
<term>Genes, Bacterial</term>
<term>Molecular Sequence Data</term>
<term>Restriction Mapping</term>
<term>Sequence Homology, Amino Acid</term>
<term>Species Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Cartographie de restriction</term>
<term>Données de séquences moléculaires</term>
<term>Gènes bactériens</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Spécificité d'espèce</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Éléments transposables d'ADN</term>
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<front><div type="abstract" xml:lang="en">A new insertion sequence, IS292, located in the 6b gene of a nopaline-type Agrobacterium strain (X88-292) isolated from poplar was identified and sequenced. IS292 is 2494 bp long, has 21 bp inverted terminal repeats with two mismatches, and generates 10 bp direct repeats upon integration. No sequence similarity was found between IS292 and other insertion elements associated with Agrobacterium, but it shows strong similarity with ISR/1 from Rhizobium leguminosarum bv. viciae. The occurrence of IS292-like sequences in various Agrobacterium isolates, especially different Agrobacterium strains isolated from the same biotope, was demonstrated by DNA hybridization.</div>
</front>
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<Title>Microbiology (Reading, England)</Title>
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<ArticleTitle>IS292: a novel insertion element from Agrobacterium.</ArticleTitle>
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<Abstract><AbstractText>A new insertion sequence, IS292, located in the 6b gene of a nopaline-type Agrobacterium strain (X88-292) isolated from poplar was identified and sequenced. IS292 is 2494 bp long, has 21 bp inverted terminal repeats with two mismatches, and generates 10 bp direct repeats upon integration. No sequence similarity was found between IS292 and other insertion elements associated with Agrobacterium, but it shows strong similarity with ISR/1 from Rhizobium leguminosarum bv. viciae. The occurrence of IS292-like sequences in various Agrobacterium isolates, especially different Agrobacterium strains isolated from the same biotope, was demonstrated by DNA hybridization.</AbstractText>
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