PoplarV1, Main, Corpus, bibRecord, 004A75

Characterization of the structure and determination of mRNA levels of the phenylalanine ammonia-lyase gene family from Populus kitakamiensis.

Identifieur interne : 004A75 ( Main/Corpus ); précédent : 004A74; suivant : 004A76

Characterization of the structure and determination of mRNA levels of the phenylalanine ammonia-lyase gene family from Populus kitakamiensis.

Auteurs : Y. Osakabe ; K. Osakabe ; S. Kawai ; Y. Katayama ; N. Morohoshi

Source :

Plant molecular biology [ 0167-4412 ] ; 1995.

RBID : pubmed:7548831

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Cloning, Molecular (MeSH), DNA Primers (chemistry), Gene Expression (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Introns (MeSH), Molecular Sequence Data (MeSH), Phenylalanine Ammonia-Lyase (genetics), Plant Proteins (genetics), Promoter Regions, Genetic (MeSH), RNA, Messenger (genetics), Trees (enzymology), Trees (genetics).
MESH :
- chemical , chemistry : DNA Primers.
- chemical , genetics : Phenylalanine Ammonia-Lyase, Plant Proteins, RNA, Messenger.
- enzymology : Trees.
- genetics : Trees.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Gene Expression, Gene Expression Regulation, Plant, Genes, Plant, Introns, Molecular Sequence Data, Promoter Regions, Genetic.

Abstract

We isolated two new PAL genes, palg2b and palg4, from Populus kitakamiensis, palg2a and palg2b are clustered and palg2b encodes a polypeptide of 710 amino acids. The nucleotide sequence in the coding region of palg2b was 94.6% identical to that of palg2a. The promoter regions of palg1, palg2a and palg2b have several elements conserved among many phenylpropanoid biosynthetic genes. We measured the mRNA levels of the four PAL genes by S1 mapping using total RNA from stem tissues developing secondary xylem. Results showed that the transcript level of palg2b was higher than that of the other PAL genes.

DOI: 10.1007/BF00032674
PubMed: 7548831

Links to Exploration step

pubmed:7548831

Le document en format XML

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<author><name sortKey="Osakabe, Y" sort="Osakabe, Y" uniqKey="Osakabe Y" first="Y" last="Osakabe">Y. Osakabe</name>
<affiliation><nlm:affiliation>Laboratory of Wood Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.</nlm:affiliation>
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<author><name sortKey="Osakabe, K" sort="Osakabe, K" uniqKey="Osakabe K" first="K" last="Osakabe">K. Osakabe</name>
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<author><name sortKey="Kawai, S" sort="Kawai, S" uniqKey="Kawai S" first="S" last="Kawai">S. Kawai</name>
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<author><name sortKey="Katayama, Y" sort="Katayama, Y" uniqKey="Katayama Y" first="Y" last="Katayama">Y. Katayama</name>
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<author><name sortKey="Morohoshi, N" sort="Morohoshi, N" uniqKey="Morohoshi N" first="N" last="Morohoshi">N. Morohoshi</name>
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<series><title level="j">Plant molecular biology</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term>
<term>Base Sequence (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>DNA Primers (chemistry)</term>
<term>Gene Expression (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Genes, Plant (MeSH)</term>
<term>Introns (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Phenylalanine Ammonia-Lyase (genetics)</term>
<term>Plant Proteins (genetics)</term>
<term>Promoter Regions, Genetic (MeSH)</term>
<term>RNA, Messenger (genetics)</term>
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</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>Cloning, Molecular</term>
<term>Gene Expression</term>
<term>Gene Expression Regulation, Plant</term>
<term>Genes, Plant</term>
<term>Introns</term>
<term>Molecular Sequence Data</term>
<term>Promoter Regions, Genetic</term>
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<front><div type="abstract" xml:lang="en">We isolated two new PAL genes, palg2b and palg4, from Populus kitakamiensis, palg2a and palg2b are clustered and palg2b encodes a polypeptide of 710 amino acids. The nucleotide sequence in the coding region of palg2b was 94.6% identical to that of palg2a. The promoter regions of palg1, palg2a and palg2b have several elements conserved among many phenylpropanoid biosynthetic genes. We measured the mRNA levels of the four PAL genes by S1 mapping using total RNA from stem tissues developing secondary xylem. Results showed that the transcript level of palg2b was higher than that of the other PAL genes.</div>
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<Abstract><AbstractText>We isolated two new PAL genes, palg2b and palg4, from Populus kitakamiensis, palg2a and palg2b are clustered and palg2b encodes a polypeptide of 710 amino acids. The nucleotide sequence in the coding region of palg2b was 94.6% identical to that of palg2a. The promoter regions of palg1, palg2a and palg2b have several elements conserved among many phenylpropanoid biosynthetic genes. We measured the mRNA levels of the four PAL genes by S1 mapping using total RNA from stem tissues developing secondary xylem. Results showed that the transcript level of palg2b was higher than that of the other PAL genes.</AbstractText>
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Characterization of the structure and determination of mRNA levels of the phenylalanine ammonia-lyase gene family from Populus kitakamiensis.

Characterization of the structure and determination of mRNA levels of the phenylalanine ammonia-lyase gene family from Populus kitakamiensis.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki