PoplarV1, Main, Corpus, bibRecord, 004830

Transformation of elite white poplar (Populus alba L.) cv. ' Villafranca' and evaluation of herbicide resistance.

Identifieur interne : 004830 ( Main/Corpus ); précédent : 004829; suivant : 004831

Transformation of elite white poplar (Populus alba L.) cv. ' Villafranca' and evaluation of herbicide resistance.

Auteurs : M. Confalonieri ; B. Belenghi ; A. Balestrazzi ; S. Negri ; G. Facciotto ; G. Schenone ; M. Delledonne

Source :

Plant cell reports [ 1432-203X ] ; 2000.

RBID : pubmed:30754842

Abstract

Transgenic white poplar plants (Populus alba L.) expressing the nptII gene and the bar gene from Streptomyces hygroscopicus have been produced using Agrobacterium tumefaciens-mediated gene transfer. Eleven kanamycin-resistant plant lines were obtained with a transformation frequency of 7%. Successful genetic transformation was confirmed by Southern and northern analyses. The level of resistance to the commercial preparation of phosphinothricin (Basta; Roussel-Hoechst Agrovet) was evaluated by in vitro and in vivo assays. Using in vitro selective conditions for phosphinothricin, only plantlets from four kanamycin-resistant independent lines remained green and continued to grow and root. After transfer to the growth chamber, all selected transgenic lines were shown to be completely resistant to the herbicide Basta with doses equivalent to 6 l ha^-1 (normal field dosage) and were tolerant at concentration of 12 l ha^-1. This is the first report describing the genetic transformation of a P. alba clonal cultivar of commercial interest with a gene of agronomic value.

DOI: 10.1007/s002990000230
PubMed: 30754842

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pubmed:30754842

Le document en format XML

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<author><name sortKey="Confalonieri, M" sort="Confalonieri, M" uniqKey="Confalonieri M" first="M" last="Confalonieri">M. Confalonieri</name>
<affiliation><nlm:affiliation>Istituto di Sperimentazione per la Pioppicoltura, Via di Frassineto 35, 15033 Casale Monferrato (AL), Italy e-mail: confalonieri@populus.it Fax: +39-0142-55580, , , , , , IT.</nlm:affiliation>
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<author><name sortKey="Belenghi, B" sort="Belenghi, B" uniqKey="Belenghi B" first="B" last="Belenghi">B. Belenghi</name>
<affiliation><nlm:affiliation>Istituto di Genetica Vegetale, Università Cattolica del S. Cuore, Via Emilia Parmense 84, 29100 Piacenza, Italy, , , , , , IT.</nlm:affiliation>
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<author><name sortKey="Balestrazzi, A" sort="Balestrazzi, A" uniqKey="Balestrazzi A" first="A" last="Balestrazzi">A. Balestrazzi</name>
<affiliation><nlm:affiliation>Dipartimento di Genetica e Microbiologia 'A. Buzzati-Traverso', Università di Pavia, Via Abbiategrasso 207, 27100 Pavia, Italy, , , , , , IT.</nlm:affiliation>
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<author><name sortKey="Negri, S" sort="Negri, S" uniqKey="Negri S" first="S" last="Negri">S. Negri</name>
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<author><name sortKey="Facciotto, G" sort="Facciotto, G" uniqKey="Facciotto G" first="G" last="Facciotto">G. Facciotto</name>
<affiliation><nlm:affiliation>Istituto di Sperimentazione per la Pioppicoltura, Via di Frassineto 35, 15033 Casale Monferrato (AL), Italy e-mail: confalonieri@populus.it Fax: +39-0142-55580, , , , , , IT.</nlm:affiliation>
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<author><name sortKey="Schenone, G" sort="Schenone, G" uniqKey="Schenone G" first="G" last="Schenone">G. Schenone</name>
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<author><name sortKey="Delledonne, M" sort="Delledonne, M" uniqKey="Delledonne M" first="M" last="Delledonne">M. Delledonne</name>
<affiliation><nlm:affiliation>Istituto di Genetica Vegetale, Università Cattolica del S. Cuore, Via Emilia Parmense 84, 29100 Piacenza, Italy, , , , , , IT.</nlm:affiliation>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Transformation of elite white poplar (Populus alba L.) cv. ' Villafranca' and evaluation of herbicide resistance.</title>
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<author><name sortKey="Belenghi, B" sort="Belenghi, B" uniqKey="Belenghi B" first="B" last="Belenghi">B. Belenghi</name>
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<author><name sortKey="Balestrazzi, A" sort="Balestrazzi, A" uniqKey="Balestrazzi A" first="A" last="Balestrazzi">A. Balestrazzi</name>
<affiliation><nlm:affiliation>Dipartimento di Genetica e Microbiologia 'A. Buzzati-Traverso', Università di Pavia, Via Abbiategrasso 207, 27100 Pavia, Italy, , , , , , IT.</nlm:affiliation>
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<author><name sortKey="Negri, S" sort="Negri, S" uniqKey="Negri S" first="S" last="Negri">S. Negri</name>
<affiliation><nlm:affiliation>Istituto di Sperimentazione per la Pioppicoltura, Via di Frassineto 35, 15033 Casale Monferrato (AL), Italy e-mail: confalonieri@populus.it Fax: +39-0142-55580, , , , , , IT.</nlm:affiliation>
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<front><div type="abstract" xml:lang="en"> Transgenic white poplar plants (Populus alba L.) expressing the nptII gene and the bar gene from Streptomyces hygroscopicus have been produced using Agrobacterium tumefaciens-mediated gene transfer. Eleven kanamycin-resistant plant lines were obtained with a transformation frequency of 7%. Successful genetic transformation was confirmed by Southern and northern analyses. The level of resistance to the commercial preparation of phosphinothricin (Basta; Roussel-Hoechst Agrovet) was evaluated by in vitro and in vivo assays. Using in vitro selective conditions for phosphinothricin, only plantlets from four kanamycin-resistant independent lines remained green and continued to grow and root. After transfer to the growth chamber, all selected transgenic lines were shown to be completely resistant to the herbicide Basta with doses equivalent to 6 l ha<sup>-1</sup>
 (normal field dosage) and were tolerant at concentration of 12 l ha<sup>-1</sup>
. This is the first report describing the genetic transformation of a P. alba clonal cultivar of commercial interest with a gene of agronomic value.</div>
</front>
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<Month>10</Month>
<Day>01</Day>
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<JournalIssue CitedMedium="Internet"><Volume>19</Volume>
<Issue>10</Issue>
<PubDate><Year>2000</Year>
<Month>Oct</Month>
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<Title>Plant cell reports</Title>
<ISOAbbreviation>Plant Cell Rep</ISOAbbreviation>
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<ArticleTitle>Transformation of elite white poplar (Populus alba L.) cv. ' Villafranca' and evaluation of herbicide resistance.</ArticleTitle>
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<Abstract><AbstractText> Transgenic white poplar plants (Populus alba L.) expressing the nptII gene and the bar gene from Streptomyces hygroscopicus have been produced using Agrobacterium tumefaciens-mediated gene transfer. Eleven kanamycin-resistant plant lines were obtained with a transformation frequency of 7%. Successful genetic transformation was confirmed by Southern and northern analyses. The level of resistance to the commercial preparation of phosphinothricin (Basta; Roussel-Hoechst Agrovet) was evaluated by in vitro and in vivo assays. Using in vitro selective conditions for phosphinothricin, only plantlets from four kanamycin-resistant independent lines remained green and continued to grow and root. After transfer to the growth chamber, all selected transgenic lines were shown to be completely resistant to the herbicide Basta with doses equivalent to 6 l ha<sup>-1</sup>
 (normal field dosage) and were tolerant at concentration of 12 l ha<sup>-1</sup>
. This is the first report describing the genetic transformation of a P. alba clonal cultivar of commercial interest with a gene of agronomic value.</AbstractText>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Confalonieri</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Istituto di Sperimentazione per la Pioppicoltura, Via di Frassineto 35, 15033 Casale Monferrato (AL), Italy e-mail: confalonieri@populus.it Fax: +39-0142-55580, , , , , , IT.</Affiliation>
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<AffiliationInfo><Affiliation>Istituto di Genetica Vegetale, Università Cattolica del S. Cuore, Via Emilia Parmense 84, 29100 Piacenza, Italy, , , , , , IT.</Affiliation>
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<Author ValidYN="Y"><LastName>Balestrazzi</LastName>
<ForeName>A</ForeName>
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<AffiliationInfo><Affiliation>Dipartimento di Genetica e Microbiologia 'A. Buzzati-Traverso', Università di Pavia, Via Abbiategrasso 207, 27100 Pavia, Italy, , , , , , IT.</Affiliation>
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<Author ValidYN="Y"><LastName>Negri</LastName>
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<AffiliationInfo><Affiliation>Istituto di Sperimentazione per la Pioppicoltura, Via di Frassineto 35, 15033 Casale Monferrato (AL), Italy e-mail: confalonieri@populus.it Fax: +39-0142-55580, , , , , , IT.</Affiliation>
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<Author ValidYN="Y"><LastName>Facciotto</LastName>
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<Initials>G</Initials>
<AffiliationInfo><Affiliation>Istituto di Sperimentazione per la Pioppicoltura, Via di Frassineto 35, 15033 Casale Monferrato (AL), Italy e-mail: confalonieri@populus.it Fax: +39-0142-55580, , , , , , IT.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Schenone</LastName>
<ForeName>G</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>ENEL Ricerca, Via Volta 1, 20093 Cologno Monzese (MI), Italy, , , , , , IT.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Delledonne</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Istituto di Genetica Vegetale, Università Cattolica del S. Cuore, Via Emilia Parmense 84, 29100 Piacenza, Italy, , , , , , IT.</Affiliation>
</AffiliationInfo>
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<Language>eng</Language>
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<MedlineJournalInfo><Country>Germany</Country>
<MedlineTA>Plant Cell Rep</MedlineTA>
<NlmUniqueID>9880970</NlmUniqueID>
<ISSNLinking>0721-7714</ISSNLinking>
</MedlineJournalInfo>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Herbicide resistance</Keyword>
<Keyword MajorTopicYN="N">Key words Agrobacterium tumefaciens</Keyword>
<Keyword MajorTopicYN="N">Populus alba L.</Keyword>
<Keyword MajorTopicYN="N">Transgenic plants</Keyword>
<Keyword MajorTopicYN="N">bar gene</Keyword>
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Transformation of elite white poplar (Populus alba L.) cv. ' Villafranca' and evaluation of herbicide resistance.

Transformation of elite white poplar (Populus alba L.) cv. ' Villafranca' and evaluation of herbicide resistance.

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Abstract

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