Recovery of useful traits from isolates inhabiting an agricultural soil cultivated with herbicide-resistant poplars.
Identifieur interne : 003925 ( Main/Corpus ); précédent : 003924; suivant : 003926Recovery of useful traits from isolates inhabiting an agricultural soil cultivated with herbicide-resistant poplars.
Auteurs : Alma Balestrazzi ; Martina Bonadei ; Samanta Zelasco ; Emanuele Quattrini ; Cinzia Calvio ; Alessandro Galizzi ; Daniela CarboneraSource :
- Canadian journal of microbiology [ 0008-4166 ] ; 2008.
English descriptors
- KwdEn :
- Aminobutyrates (pharmacology), Bacillus (classification), Bacillus (genetics), Bacillus (isolation & purification), Bacillus (metabolism), Colony Count, Microbial (MeSH), DNA, Bacterial (chemistry), DNA, Bacterial (genetics), DNA, Ribosomal (chemistry), DNA, Ribosomal (genetics), Herbicides (pharmacology), Indoleacetic Acids (metabolism), Indoleacetic Acids (pharmacology), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Plant Development (MeSH), Plants (drug effects), Populus (growth & development), Pseudomonas (classification), Pseudomonas (genetics), Pseudomonas (isolation & purification), Pseudomonas (metabolism), RNA, Ribosomal, 16S (genetics), Sequence Analysis, DNA (MeSH), Sequence Homology, Nucleic Acid (MeSH), Soil Microbiology (MeSH).
- MESH :
- chemical , chemistry : DNA, Bacterial, DNA, Ribosomal.
- chemical , genetics : DNA, Bacterial, DNA, Ribosomal, RNA, Ribosomal, 16S.
- chemical , metabolism : Indoleacetic Acids.
- chemical , pharmacology : Aminobutyrates, Herbicides, Indoleacetic Acids.
- classification : Bacillus, Pseudomonas.
- drug effects : Plants.
- genetics : Bacillus, Pseudomonas.
- growth & development : Populus.
- isolation & purification : Bacillus, Pseudomonas.
- metabolism : Bacillus, Pseudomonas.
- Colony Count, Microbial, Molecular Sequence Data, Phylogeny, Plant Development, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Soil Microbiology.
Abstract
The aim of this study was to investigate the culturable bacteria living in soil cultivated with Basta-tolerant transgenic white poplars (Populus alba L. 'Villafranca'). Plate Count Agar medium containing phosphinothricin, the active component of Basta, was used to isolate the herbicide-resistant bacteria (HRB). No significant changes in the size of the soil microbial flora following herbicide treatment were observed. The characterization of HRB isolates by 16S rDNA-based taxonomy revealed a predominance of Pseudomonas and Bacillus species. The screening carried out on soil samples allowed for the recovery of isolates with useful properties for biotechnological and agronomical purposes, particularly in relation to root development. Among the tested isolates, only HRB-1b, HRB-1c, and HRB-7 showed remarkable swarming ability, a valuable trait supporting the beneficial plant-microbe interactions. HRB-1c was also characterized by consistent production of indoleacetic acid (17.8 +/- 0.09 microg x mL-1 x (OD600 unit)-1), and it was able to stimulate the in vitro growth of Villafranca explants. Since novel tools are constantly required to enhance productivity of perennial species and to expand their use for practical purposes, the availability of bacteria that support tree growth, such as the HRB-1c isolate, represents a significant advantage.
DOI: 10.1139/w07-136
PubMed: 18388991
Links to Exploration step
pubmed:18388991Le document en format XML
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sort="Calvio, Cinzia" uniqKey="Calvio C" first="Cinzia" last="Calvio">Cinzia Calvio</name></author><author><name sortKey="Galizzi, Alessandro" sort="Galizzi, Alessandro" uniqKey="Galizzi A" first="Alessandro" last="Galizzi">Alessandro Galizzi</name></author><author><name sortKey="Carbonera, Daniela" sort="Carbonera, Daniela" uniqKey="Carbonera D" first="Daniela" last="Carbonera">Daniela Carbonera</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18388991</idno><idno type="pmid">18388991</idno><idno type="doi">10.1139/w07-136</idno><idno type="wicri:Area/Main/Corpus">003925</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003925</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Recovery of useful traits from isolates inhabiting an agricultural soil cultivated with herbicide-resistant poplars.</title><author><name sortKey="Balestrazzi, Alma" sort="Balestrazzi, Alma" uniqKey="Balestrazzi A" first="Alma" last="Balestrazzi">Alma Balestrazzi</name><affiliation><nlm:affiliation>Department of Genetics and Microbiology, University of Pavia, via Ferrata 1, Pavia 27100, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Bonadei, Martina" sort="Bonadei, Martina" uniqKey="Bonadei M" first="Martina" last="Bonadei">Martina Bonadei</name></author><author><name sortKey="Zelasco, Samanta" sort="Zelasco, Samanta" uniqKey="Zelasco S" first="Samanta" last="Zelasco">Samanta Zelasco</name></author><author><name sortKey="Quattrini, Emanuele" sort="Quattrini, Emanuele" uniqKey="Quattrini E" first="Emanuele" last="Quattrini">Emanuele Quattrini</name></author><author><name sortKey="Calvio, Cinzia" sort="Calvio, Cinzia" uniqKey="Calvio C" first="Cinzia" last="Calvio">Cinzia Calvio</name></author><author><name sortKey="Galizzi, Alessandro" sort="Galizzi, Alessandro" uniqKey="Galizzi A" first="Alessandro" last="Galizzi">Alessandro Galizzi</name></author><author><name sortKey="Carbonera, Daniela" sort="Carbonera, Daniela" uniqKey="Carbonera D" first="Daniela" last="Carbonera">Daniela Carbonera</name></author></analytic><series><title level="j">Canadian journal of microbiology</title><idno type="ISSN">0008-4166</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aminobutyrates (pharmacology)</term><term>Bacillus (classification)</term><term>Bacillus (genetics)</term><term>Bacillus (isolation & purification)</term><term>Bacillus (metabolism)</term><term>Colony Count, Microbial (MeSH)</term><term>DNA, Bacterial (chemistry)</term><term>DNA, Bacterial (genetics)</term><term>DNA, Ribosomal (chemistry)</term><term>DNA, Ribosomal (genetics)</term><term>Herbicides (pharmacology)</term><term>Indoleacetic Acids (metabolism)</term><term>Indoleacetic Acids (pharmacology)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plant Development (MeSH)</term><term>Plants (drug effects)</term><term>Populus (growth & development)</term><term>Pseudomonas (classification)</term><term>Pseudomonas (genetics)</term><term>Pseudomonas (isolation & purification)</term><term>Pseudomonas (metabolism)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Sequence Homology, Nucleic Acid (MeSH)</term><term>Soil Microbiology (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Bacterial</term><term>DNA, Ribosomal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term><term>DNA, Ribosomal</term><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Indoleacetic 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Data</term><term>Phylogeny</term><term>Plant Development</term><term>Sequence Analysis, DNA</term><term>Sequence Homology, Nucleic Acid</term><term>Soil Microbiology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this study was to investigate the culturable bacteria living in soil cultivated with Basta-tolerant transgenic white poplars (Populus alba L. 'Villafranca'). Plate Count Agar medium containing phosphinothricin, the active component of Basta, was used to isolate the herbicide-resistant bacteria (HRB). No significant changes in the size of the soil microbial flora following herbicide treatment were observed. The characterization of HRB isolates by 16S rDNA-based taxonomy revealed a predominance of Pseudomonas and Bacillus species. The screening carried out on soil samples allowed for the recovery of isolates with useful properties for biotechnological and agronomical purposes, particularly in relation to root development. Among the tested isolates, only HRB-1b, HRB-1c, and HRB-7 showed remarkable swarming ability, a valuable trait supporting the beneficial plant-microbe interactions. HRB-1c was also characterized by consistent production of indoleacetic acid (17.8 +/- 0.09 microg x mL-1 x (OD600 unit)-1), and it was able to stimulate the in vitro growth of Villafranca explants. Since novel tools are constantly required to enhance productivity of perennial species and to expand their use for practical purposes, the availability of bacteria that support tree growth, such as the HRB-1c isolate, represents a significant advantage.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18388991</PMID><DateCompleted><Year>2008</Year><Month>05</Month><Day>22</Day></DateCompleted><DateRevised><Year>2012</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0008-4166</ISSN><JournalIssue CitedMedium="Print"><Volume>54</Volume><Issue>3</Issue><PubDate><Year>2008</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Canadian journal of microbiology</Title><ISOAbbreviation>Can J Microbiol</ISOAbbreviation></Journal><ArticleTitle>Recovery of useful traits from isolates inhabiting an agricultural soil cultivated with herbicide-resistant poplars.</ArticleTitle><Pagination><MedlinePgn>201-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1139/w07-136</ELocationID><Abstract><AbstractText>The aim of this study was to investigate the culturable bacteria living in soil cultivated with Basta-tolerant transgenic white poplars (Populus alba L. 'Villafranca'). Plate Count Agar medium containing phosphinothricin, the active component of Basta, was used to isolate the herbicide-resistant bacteria (HRB). No significant changes in the size of the soil microbial flora following herbicide treatment were observed. The characterization of HRB isolates by 16S rDNA-based taxonomy revealed a predominance of Pseudomonas and Bacillus species. The screening carried out on soil samples allowed for the recovery of isolates with useful properties for biotechnological and agronomical purposes, particularly in relation to root development. Among the tested isolates, only HRB-1b, HRB-1c, and HRB-7 showed remarkable swarming ability, a valuable trait supporting the beneficial plant-microbe interactions. HRB-1c was also characterized by consistent production of indoleacetic acid (17.8 +/- 0.09 microg x mL-1 x (OD600 unit)-1), and it was able to stimulate the in vitro growth of Villafranca explants. 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ValidYN="Y"><LastName>Calvio</LastName><ForeName>Cinzia</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Galizzi</LastName><ForeName>Alessandro</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Carbonera</LastName><ForeName>Daniela</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><DataBankList 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