Functional analysis of PsG6PDH, a cytosolic glucose-6-phosphate dehydrogenase gene from Populus suaveolens, and its contribution to cold tolerance improvement in tobacco plants.
Identifieur interne : 002592 ( Main/Corpus ); précédent : 002591; suivant : 002593Functional analysis of PsG6PDH, a cytosolic glucose-6-phosphate dehydrogenase gene from Populus suaveolens, and its contribution to cold tolerance improvement in tobacco plants.
Auteurs : Yuanzhen Lin ; Shanzhi Lin ; Hai Guo ; Zhiyi Zhang ; Xiaoyang ChenSource :
- Biotechnology letters [ 1573-6776 ] ; 2013.
English descriptors
- KwdEn :
- Cold Temperature (MeSH), Gene Expression (MeSH), Gene Expression Profiling (MeSH), Glucosephosphate Dehydrogenase (genetics), Glucosephosphate Dehydrogenase (metabolism), Malondialdehyde (analysis), Peroxides (analysis), Phylogeny (MeSH), Plant Leaves (chemistry), Plants, Genetically Modified (MeSH), Populus (enzymology), Populus (genetics), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Sequence Alignment (MeSH), Stress, Physiological (MeSH), Superoxide Dismutase (analysis), Tobacco (enzymology), Tobacco (genetics), Tobacco (radiation effects).
- MESH :
- chemical , analysis : Malondialdehyde, Peroxides, Superoxide Dismutase.
- chemical , genetics : Glucosephosphate Dehydrogenase, Recombinant Proteins.
- chemical , metabolism : Glucosephosphate Dehydrogenase, Recombinant Proteins.
- chemistry : Plant Leaves.
- enzymology : Populus, Tobacco.
- genetics : Populus, Tobacco.
- radiation effects : Tobacco.
- Cold Temperature, Gene Expression, Gene Expression Profiling, Phylogeny, Plants, Genetically Modified, Sequence Alignment, Stress, Physiological.
Abstract
A 1,697-bp cDNA sequence, designated as PsG6PDH, was amplified from Populus suaveolens. Multiple sequence alignment and phylogenetic analysis indicated that PsG6PDH encodes a cytosolic G6PDH isoform, with Southern blot analysis demonstrating that the gene is single or low copy in Populus. Transgenic tobacco plants over-expressing PsG6PDH exhibited enhanced cold tolerance. In both transgenic and wild-type (WT) tobacco plants, cold stress increased leaf malondialdehyde (MDA) content, electrolyte leakage (EL), and peroxide (POD) and superoxide dismutase (SOD) activities; relative to WT, however, transgenic lines had lower MDA content and EL and higher SOD and POD activities. In addition, PsG6PDH activated the expression of stress-related genes, including NtERD10b, NtERD10c, and NtSOD, in tobacco plants. Our results provide evidence regarding PsG6PDH regulatory function in plants during low temperature stress.
DOI: 10.1007/s10529-013-1226-2
PubMed: 23690038
Links to Exploration step
pubmed:23690038Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Functional analysis of PsG6PDH, a cytosolic glucose-6-phosphate dehydrogenase gene from Populus suaveolens, and its contribution to cold tolerance improvement in tobacco plants.</title>
<author><name sortKey="Lin, Yuanzhen" sort="Lin, Yuanzhen" uniqKey="Lin Y" first="Yuanzhen" last="Lin">Yuanzhen Lin</name>
<affiliation><nlm:affiliation>College of Forestry, South China Agricultural University, Guangzhou, 510642, China. yzhlin@scau.edu.cn</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Lin, Shanzhi" sort="Lin, Shanzhi" uniqKey="Lin S" first="Shanzhi" last="Lin">Shanzhi Lin</name>
</author>
<author><name sortKey="Guo, Hai" sort="Guo, Hai" uniqKey="Guo H" first="Hai" last="Guo">Hai Guo</name>
</author>
<author><name sortKey="Zhang, Zhiyi" sort="Zhang, Zhiyi" uniqKey="Zhang Z" first="Zhiyi" last="Zhang">Zhiyi Zhang</name>
</author>
<author><name sortKey="Chen, Xiaoyang" sort="Chen, Xiaoyang" uniqKey="Chen X" first="Xiaoyang" last="Chen">Xiaoyang Chen</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2013">2013</date>
<idno type="RBID">pubmed:23690038</idno>
<idno type="pmid">23690038</idno>
<idno type="doi">10.1007/s10529-013-1226-2</idno>
<idno type="wicri:Area/Main/Corpus">002592</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002592</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Functional analysis of PsG6PDH, a cytosolic glucose-6-phosphate dehydrogenase gene from Populus suaveolens, and its contribution to cold tolerance improvement in tobacco plants.</title>
<author><name sortKey="Lin, Yuanzhen" sort="Lin, Yuanzhen" uniqKey="Lin Y" first="Yuanzhen" last="Lin">Yuanzhen Lin</name>
<affiliation><nlm:affiliation>College of Forestry, South China Agricultural University, Guangzhou, 510642, China. yzhlin@scau.edu.cn</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Lin, Shanzhi" sort="Lin, Shanzhi" uniqKey="Lin S" first="Shanzhi" last="Lin">Shanzhi Lin</name>
</author>
<author><name sortKey="Guo, Hai" sort="Guo, Hai" uniqKey="Guo H" first="Hai" last="Guo">Hai Guo</name>
</author>
<author><name sortKey="Zhang, Zhiyi" sort="Zhang, Zhiyi" uniqKey="Zhang Z" first="Zhiyi" last="Zhang">Zhiyi Zhang</name>
</author>
<author><name sortKey="Chen, Xiaoyang" sort="Chen, Xiaoyang" uniqKey="Chen X" first="Xiaoyang" last="Chen">Xiaoyang Chen</name>
</author>
</analytic>
<series><title level="j">Biotechnology letters</title>
<idno type="eISSN">1573-6776</idno>
<imprint><date when="2013" type="published">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cold Temperature (MeSH)</term>
<term>Gene Expression (MeSH)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Glucosephosphate Dehydrogenase (genetics)</term>
<term>Glucosephosphate Dehydrogenase (metabolism)</term>
<term>Malondialdehyde (analysis)</term>
<term>Peroxides (analysis)</term>
<term>Phylogeny (MeSH)</term>
<term>Plant Leaves (chemistry)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Populus (enzymology)</term>
<term>Populus (genetics)</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (metabolism)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Stress, Physiological (MeSH)</term>
<term>Superoxide Dismutase (analysis)</term>
<term>Tobacco (enzymology)</term>
<term>Tobacco (genetics)</term>
<term>Tobacco (radiation effects)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Malondialdehyde</term>
<term>Peroxides</term>
<term>Superoxide Dismutase</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glucosephosphate Dehydrogenase</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucosephosphate Dehydrogenase</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plant Leaves</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term>
<term>Tobacco</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term>
<term>Tobacco</term>
</keywords>
<keywords scheme="MESH" qualifier="radiation effects" xml:lang="en"><term>Tobacco</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cold Temperature</term>
<term>Gene Expression</term>
<term>Gene Expression Profiling</term>
<term>Phylogeny</term>
<term>Plants, Genetically Modified</term>
<term>Sequence Alignment</term>
<term>Stress, Physiological</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A 1,697-bp cDNA sequence, designated as PsG6PDH, was amplified from Populus suaveolens. Multiple sequence alignment and phylogenetic analysis indicated that PsG6PDH encodes a cytosolic G6PDH isoform, with Southern blot analysis demonstrating that the gene is single or low copy in Populus. Transgenic tobacco plants over-expressing PsG6PDH exhibited enhanced cold tolerance. In both transgenic and wild-type (WT) tobacco plants, cold stress increased leaf malondialdehyde (MDA) content, electrolyte leakage (EL), and peroxide (POD) and superoxide dismutase (SOD) activities; relative to WT, however, transgenic lines had lower MDA content and EL and higher SOD and POD activities. In addition, PsG6PDH activated the expression of stress-related genes, including NtERD10b, NtERD10c, and NtSOD, in tobacco plants. Our results provide evidence regarding PsG6PDH regulatory function in plants during low temperature stress. </div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23690038</PMID>
<DateCompleted><Year>2014</Year>
<Month>02</Month>
<Day>07</Day>
</DateCompleted>
<DateRevised><Year>2013</Year>
<Month>08</Month>
<Day>01</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-6776</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>35</Volume>
<Issue>9</Issue>
<PubDate><Year>2013</Year>
<Month>Sep</Month>
</PubDate>
</JournalIssue>
<Title>Biotechnology letters</Title>
<ISOAbbreviation>Biotechnol Lett</ISOAbbreviation>
</Journal>
<ArticleTitle>Functional analysis of PsG6PDH, a cytosolic glucose-6-phosphate dehydrogenase gene from Populus suaveolens, and its contribution to cold tolerance improvement in tobacco plants.</ArticleTitle>
<Pagination><MedlinePgn>1509-18</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1007/s10529-013-1226-2</ELocationID>
<Abstract><AbstractText>A 1,697-bp cDNA sequence, designated as PsG6PDH, was amplified from Populus suaveolens. Multiple sequence alignment and phylogenetic analysis indicated that PsG6PDH encodes a cytosolic G6PDH isoform, with Southern blot analysis demonstrating that the gene is single or low copy in Populus. Transgenic tobacco plants over-expressing PsG6PDH exhibited enhanced cold tolerance. In both transgenic and wild-type (WT) tobacco plants, cold stress increased leaf malondialdehyde (MDA) content, electrolyte leakage (EL), and peroxide (POD) and superoxide dismutase (SOD) activities; relative to WT, however, transgenic lines had lower MDA content and EL and higher SOD and POD activities. In addition, PsG6PDH activated the expression of stress-related genes, including NtERD10b, NtERD10c, and NtSOD, in tobacco plants. Our results provide evidence regarding PsG6PDH regulatory function in plants during low temperature stress. </AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lin</LastName>
<ForeName>Yuanzhen</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>College of Forestry, South China Agricultural University, Guangzhou, 510642, China. yzhlin@scau.edu.cn</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lin</LastName>
<ForeName>Shanzhi</ForeName>
<Initials>S</Initials>
</Author>
<Author ValidYN="Y"><LastName>Guo</LastName>
<ForeName>Hai</ForeName>
<Initials>H</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Zhiyi</ForeName>
<Initials>Z</Initials>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Xiaoyang</ForeName>
<Initials>X</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2013</Year>
<Month>05</Month>
<Day>21</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>Biotechnol Lett</MedlineTA>
<NlmUniqueID>8008051</NlmUniqueID>
<ISSNLinking>0141-5492</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010545">Peroxides</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>4Y8F71G49Q</RegistryNumber>
<NameOfSubstance UI="D008315">Malondialdehyde</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 1.1.1.49</RegistryNumber>
<NameOfSubstance UI="D005954">Glucosephosphate Dehydrogenase</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 1.15.1.1</RegistryNumber>
<NameOfSubstance UI="D013482">Superoxide Dismutase</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D003080" MajorTopicYN="N">Cold Temperature</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005954" MajorTopicYN="N">Glucosephosphate Dehydrogenase</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008315" MajorTopicYN="N">Malondialdehyde</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010545" MajorTopicYN="N">Peroxides</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="Y">Plants, Genetically Modified</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
<QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013312" MajorTopicYN="Y">Stress, Physiological</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013482" MajorTopicYN="N">Superoxide Dismutase</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName>
<QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000528" MajorTopicYN="Y">radiation effects</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year>
<Month>01</Month>
<Day>14</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2013</Year>
<Month>05</Month>
<Day>01</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2013</Year>
<Month>5</Month>
<Day>22</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2013</Year>
<Month>5</Month>
<Day>22</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2014</Year>
<Month>2</Month>
<Day>8</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">23690038</ArticleId>
<ArticleId IdType="doi">10.1007/s10529-013-1226-2</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002592 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 002592 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Bois |area= PoplarV1 |flux= Main |étape= Corpus |type= RBID |clé= pubmed:23690038 |texte= Functional analysis of PsG6PDH, a cytosolic glucose-6-phosphate dehydrogenase gene from Populus suaveolens, and its contribution to cold tolerance improvement in tobacco plants. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:23690038" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a PoplarV1
![]() | This area was generated with Dilib version V0.6.37. | ![]() |