Identification of defence-related cell wall proteins in Phytophthora sojae-infected soybean roots by ESI-MS/MS.
Identifieur interne : 002605 ( Main/Exploration ); précédent : 002604; suivant : 002606Identification of defence-related cell wall proteins in Phytophthora sojae-infected soybean roots by ESI-MS/MS.
Auteurs : Axel Mithöfer [Allemagne] ; Bernd Müller ; Gerhard Wanner ; Lutz A. EichackerSource :
- Molecular plant pathology [ 1364-3703 ] ; 2002.
Abstract
Summary Root cell wall proteins involved in the incompatible interaction between soybean (Glycine max L.) and the phytopathogenic oomycete Phytophthora sojae were investigated by a proteomic approach using ESI-MS/MS analysis. Success of infection and subsequently induced defence reactions was proven by staining the cell wall localized defence-related 1,3-beta-glucan, callose, in semi-thin sections of the roots. Cell wall proteins of roots were extracted three weeks post-infection, separated by SDS-PAGE and eight selected protein bands were digested with trypsin. The resulting peptides were subjected to mass spectrometry-based sequencing. For four proteins, amino acid sequence information was obtained and used to identify the corresponding proteins by a homology search in databases. All four proteins (cationic peroxidase, peroxidase precursor, amine oxidase and lipoxygenase) were defence-related and their involvement in the oxidative burst is discussed. Three of these proteins have not been described for soybean before.
DOI: 10.1046/j.1364-3703.2002.00109.x
PubMed: 20569322
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For four proteins, amino acid sequence information was obtained and used to identify the corresponding proteins by a homology search in databases. All four proteins (cationic peroxidase, peroxidase precursor, amine oxidase and lipoxygenase) were defence-related and their involvement in the oxidative burst is discussed. Three of these proteins have not been described for soybean before.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">20569322</PMID><DateCompleted><Year>2012</Year><Month>10</Month><Day>02</Day></DateCompleted><DateRevised><Year>2010</Year><Month>06</Month><Day>23</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1364-3703</ISSN><JournalIssue CitedMedium="Internet"><Volume>3</Volume><Issue>3</Issue><PubDate><Year>2002</Year><Month>May</Month><Day>01</Day></PubDate></JournalIssue><Title>Molecular plant pathology</Title><ISOAbbreviation>Mol Plant Pathol</ISOAbbreviation></Journal><ArticleTitle>Identification of defence-related cell wall proteins in Phytophthora sojae-infected soybean roots by ESI-MS/MS.</ArticleTitle><Pagination><MedlinePgn>163-6</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1046/j.1364-3703.2002.00109.x</ELocationID><Abstract><AbstractText>Summary Root cell wall proteins involved in the incompatible interaction between soybean (Glycine max L.) and the phytopathogenic oomycete Phytophthora sojae were investigated by a proteomic approach using ESI-MS/MS analysis. Success of infection and subsequently induced defence reactions was proven by staining the cell wall localized defence-related 1,3-beta-glucan, callose, in semi-thin sections of the roots. Cell wall proteins of roots were extracted three weeks post-infection, separated by SDS-PAGE and eight selected protein bands were digested with trypsin. The resulting peptides were subjected to mass spectrometry-based sequencing. For four proteins, amino acid sequence information was obtained and used to identify the corresponding proteins by a homology search in databases. All four proteins (cationic peroxidase, peroxidase precursor, amine oxidase and lipoxygenase) were defence-related and their involvement in the oxidative burst is discussed. 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