Identification of defence-related cell wall proteins in Phytophthora sojae-infected soybean roots by ESI-MS/MS.
Identifieur interne : 002684 ( Main/Curation ); précédent : 002683; suivant : 002685Identification of defence-related cell wall proteins in Phytophthora sojae-infected soybean roots by ESI-MS/MS.
Auteurs : Axel Mithöfer [Allemagne] ; Bernd Müller ; Gerhard Wanner ; Lutz A. EichackerSource :
- Molecular plant pathology [ 1364-3703 ] ; 2002.
Abstract
Summary Root cell wall proteins involved in the incompatible interaction between soybean (Glycine max L.) and the phytopathogenic oomycete Phytophthora sojae were investigated by a proteomic approach using ESI-MS/MS analysis. Success of infection and subsequently induced defence reactions was proven by staining the cell wall localized defence-related 1,3-beta-glucan, callose, in semi-thin sections of the roots. Cell wall proteins of roots were extracted three weeks post-infection, separated by SDS-PAGE and eight selected protein bands were digested with trypsin. The resulting peptides were subjected to mass spectrometry-based sequencing. For four proteins, amino acid sequence information was obtained and used to identify the corresponding proteins by a homology search in databases. All four proteins (cationic peroxidase, peroxidase precursor, amine oxidase and lipoxygenase) were defence-related and their involvement in the oxidative burst is discussed. Three of these proteins have not been described for soybean before.
DOI: 10.1046/j.1364-3703.2002.00109.x
PubMed: 20569322
Links toward previous steps (curation, corpus...)
- to stream Main, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002684
Links to Exploration step
pubmed:20569322Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of defence-related cell wall proteins in Phytophthora sojae-infected soybean roots by ESI-MS/MS.</title>
<author><name sortKey="Mithofer, Axel" sort="Mithofer, Axel" uniqKey="Mithofer A" first="Axel" last="Mithöfer">Axel Mithöfer</name>
<affiliation wicri:level="1"><nlm:affiliation>Department für Biologie I der Universität, Botanik, Menzinger Str. 67, D-80638 München, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Department für Biologie I der Universität, Botanik, Menzinger Str. 67, D-80638 München</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Muller, Bernd" sort="Muller, Bernd" uniqKey="Muller B" first="Bernd" last="Müller">Bernd Müller</name>
</author>
<author><name sortKey="Wanner, Gerhard" sort="Wanner, Gerhard" uniqKey="Wanner G" first="Gerhard" last="Wanner">Gerhard Wanner</name>
</author>
<author><name sortKey="Eichacker, Lutz A" sort="Eichacker, Lutz A" uniqKey="Eichacker L" first="Lutz A" last="Eichacker">Lutz A. Eichacker</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2002">2002</date>
<idno type="RBID">pubmed:20569322</idno>
<idno type="pmid">20569322</idno>
<idno type="doi">10.1046/j.1364-3703.2002.00109.x</idno>
<idno type="wicri:Area/Main/Corpus">002684</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002684</idno>
<idno type="wicri:Area/Main/Curation">002684</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002684</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of defence-related cell wall proteins in Phytophthora sojae-infected soybean roots by ESI-MS/MS.</title>
<author><name sortKey="Mithofer, Axel" sort="Mithofer, Axel" uniqKey="Mithofer A" first="Axel" last="Mithöfer">Axel Mithöfer</name>
<affiliation wicri:level="1"><nlm:affiliation>Department für Biologie I der Universität, Botanik, Menzinger Str. 67, D-80638 München, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Department für Biologie I der Universität, Botanik, Menzinger Str. 67, D-80638 München</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Muller, Bernd" sort="Muller, Bernd" uniqKey="Muller B" first="Bernd" last="Müller">Bernd Müller</name>
</author>
<author><name sortKey="Wanner, Gerhard" sort="Wanner, Gerhard" uniqKey="Wanner G" first="Gerhard" last="Wanner">Gerhard Wanner</name>
</author>
<author><name sortKey="Eichacker, Lutz A" sort="Eichacker, Lutz A" uniqKey="Eichacker L" first="Lutz A" last="Eichacker">Lutz A. Eichacker</name>
</author>
</analytic>
<series><title level="j">Molecular plant pathology</title>
<idno type="eISSN">1364-3703</idno>
<imprint><date when="2002" type="published">2002</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Summary Root cell wall proteins involved in the incompatible interaction between soybean (Glycine max L.) and the phytopathogenic oomycete Phytophthora sojae were investigated by a proteomic approach using ESI-MS/MS analysis. Success of infection and subsequently induced defence reactions was proven by staining the cell wall localized defence-related 1,3-beta-glucan, callose, in semi-thin sections of the roots. Cell wall proteins of roots were extracted three weeks post-infection, separated by SDS-PAGE and eight selected protein bands were digested with trypsin. The resulting peptides were subjected to mass spectrometry-based sequencing. For four proteins, amino acid sequence information was obtained and used to identify the corresponding proteins by a homology search in databases. All four proteins (cationic peroxidase, peroxidase precursor, amine oxidase and lipoxygenase) were defence-related and their involvement in the oxidative burst is discussed. Three of these proteins have not been described for soybean before.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">20569322</PMID>
<DateCompleted><Year>2012</Year>
<Month>10</Month>
<Day>02</Day>
</DateCompleted>
<DateRevised><Year>2010</Year>
<Month>06</Month>
<Day>23</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1364-3703</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>3</Volume>
<Issue>3</Issue>
<PubDate><Year>2002</Year>
<Month>May</Month>
<Day>01</Day>
</PubDate>
</JournalIssue>
<Title>Molecular plant pathology</Title>
<ISOAbbreviation>Mol Plant Pathol</ISOAbbreviation>
</Journal>
<ArticleTitle>Identification of defence-related cell wall proteins in Phytophthora sojae-infected soybean roots by ESI-MS/MS.</ArticleTitle>
<Pagination><MedlinePgn>163-6</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1046/j.1364-3703.2002.00109.x</ELocationID>
<Abstract><AbstractText>Summary Root cell wall proteins involved in the incompatible interaction between soybean (Glycine max L.) and the phytopathogenic oomycete Phytophthora sojae were investigated by a proteomic approach using ESI-MS/MS analysis. Success of infection and subsequently induced defence reactions was proven by staining the cell wall localized defence-related 1,3-beta-glucan, callose, in semi-thin sections of the roots. Cell wall proteins of roots were extracted three weeks post-infection, separated by SDS-PAGE and eight selected protein bands were digested with trypsin. The resulting peptides were subjected to mass spectrometry-based sequencing. For four proteins, amino acid sequence information was obtained and used to identify the corresponding proteins by a homology search in databases. All four proteins (cationic peroxidase, peroxidase precursor, amine oxidase and lipoxygenase) were defence-related and their involvement in the oxidative burst is discussed. Three of these proteins have not been described for soybean before.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mithöfer</LastName>
<ForeName>Axel</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Department für Biologie I der Universität, Botanik, Menzinger Str. 67, D-80638 München, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Müller</LastName>
<ForeName>Bernd</ForeName>
<Initials>B</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wanner</LastName>
<ForeName>Gerhard</ForeName>
<Initials>G</Initials>
</Author>
<Author ValidYN="Y"><LastName>Eichacker</LastName>
<ForeName>Lutz A</ForeName>
<Initials>LA</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Mol Plant Pathol</MedlineTA>
<NlmUniqueID>100954969</NlmUniqueID>
<ISSNLinking>1364-3703</ISSNLinking>
</MedlineJournalInfo>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year>
<Month>6</Month>
<Day>24</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2002</Year>
<Month>5</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2002</Year>
<Month>5</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>1</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">20569322</ArticleId>
<ArticleId IdType="pii">MPP109</ArticleId>
<ArticleId IdType="doi">10.1046/j.1364-3703.2002.00109.x</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002684 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 002684 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Bois |area= PhytophthoraV1 |flux= Main |étape= Curation |type= RBID |clé= pubmed:20569322 |texte= Identification of defence-related cell wall proteins in Phytophthora sojae-infected soybean roots by ESI-MS/MS. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:20569322" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \ | NlmPubMed2Wicri -a PhytophthoraV1
This area was generated with Dilib version V0.6.38. |