PhanerochaeteV1, Main, Exploration, bibRecord, 000647

Cloning and homologous expression of novel lignin peroxidase genes in the white-rot fungus Phanerochaete sordida YK-624.

Identifieur interne : 000647 ( Main/Exploration ); précédent : 000646; suivant : 000648

Cloning and homologous expression of novel lignin peroxidase genes in the white-rot fungus Phanerochaete sordida YK-624.

Auteurs : Tatsuki Sugiura [Japon] ; Kenji Yamagishi ; Toshiyuki Kimura ; Tomoaki Nishida ; Hirokazu Kawagishi ; Hirofumi Hirai

Source :

Bioscience, biotechnology, and biochemistry [ 1347-6947 ] ; 2009.

RBID : pubmed:19661691

Descripteurs français

English descriptors

KwdEn :
- Cloning, Molecular (MeSH), DNA, Complementary (genetics), DNA, Fungal (MeSH), Gene Expression (MeSH), Genome, Fungal (genetics), Molecular Sequence Data (MeSH), Peroxidases (biosynthesis), Peroxidases (genetics), Phanerochaete (genetics), Plasmids (genetics), Polymerase Chain Reaction (MeSH), Recombinant Proteins (biosynthesis), Recombinant Proteins (genetics), Transformation, Genetic (MeSH).
MESH :
- chemical , biosynthesis : Peroxidases, Recombinant Proteins.
- chemical , genetics : DNA, Complementary, Peroxidases, Recombinant Proteins.
- genetics : Genome, Fungal, Phanerochaete, Plasmids.
- Cloning, Molecular, DNA, Fungal, Gene Expression, Molecular Sequence Data, Polymerase Chain Reaction, Transformation, Genetic.

Abstract

Two genes, encoding YK-LiP1 and YK-LiP2, were cloned from the white-rot fungus Phanerochaete sordida YK-624, and a homologous expression system for the gene was constructed. Two full-length cDNAs (ylpA and ylpB) were isolated by degenerate RT-PCR and RACE-PCR. The results of N-terminal amino acid sequence analysis of native YK-LiP1 and YK-LiP2 showed that ylpA and ylpB coded for YK-LiP2 and YK-LiP1 respectively. The promoter of glyceraldehyde-3-phosphate dehydrogenase cloned from P. sordida YK-624 (PsGPD) was used to drive the expression of ylpA. Expression vector pGPD-g-ylpA was transformed into a P. sordida YK-624 uracil auxotrophic mutant, UV-64. The YlpA protein was secreted in active form by the transformants after 4 d of growth in a medium containing an excessive nitrogen source, whereas endogenous YK-LiP1 and YK-LiP2 were not produced. The physical and catalytic properties of the purified YlpA protein were very similar to those of YK-LiP2. These results suggest that homologous expression of recombinant YK-LiP2 was successful.

DOI: 10.1271/bbb.90152
PubMed: 19661691

Affiliations:

Japon

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Le document en format XML

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<author><name sortKey="Sugiura, Tatsuki" sort="Sugiura, Tatsuki" uniqKey="Sugiura T" first="Tatsuki" last="Sugiura">Tatsuki Sugiura</name>
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<country xml:lang="fr">Japon</country>
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<author><name sortKey="Yamagishi, Kenji" sort="Yamagishi, Kenji" uniqKey="Yamagishi K" first="Kenji" last="Yamagishi">Kenji Yamagishi</name>
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<author><name sortKey="Kimura, Toshiyuki" sort="Kimura, Toshiyuki" uniqKey="Kimura T" first="Toshiyuki" last="Kimura">Toshiyuki Kimura</name>
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<author><name sortKey="Nishida, Tomoaki" sort="Nishida, Tomoaki" uniqKey="Nishida T" first="Tomoaki" last="Nishida">Tomoaki Nishida</name>
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<author><name sortKey="Kawagishi, Hirokazu" sort="Kawagishi, Hirokazu" uniqKey="Kawagishi H" first="Hirokazu" last="Kawagishi">Hirokazu Kawagishi</name>
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<series><title level="j">Bioscience, biotechnology, and biochemistry</title>
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<term>Gene Expression (MeSH)</term>
<term>Genome, Fungal (genetics)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Peroxidases (biosynthesis)</term>
<term>Peroxidases (genetics)</term>
<term>Phanerochaete (genetics)</term>
<term>Plasmids (genetics)</term>
<term>Polymerase Chain Reaction (MeSH)</term>
<term>Recombinant Proteins (biosynthesis)</term>
<term>Recombinant Proteins (genetics)</term>
<term>Transformation, Genetic (MeSH)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term>
<term>ADN fongique (MeSH)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Expression des gènes (MeSH)</term>
<term>Génome fongique (génétique)</term>
<term>Peroxidases (biosynthèse)</term>
<term>Peroxidases (génétique)</term>
<term>Phanerochaete (génétique)</term>
<term>Plasmides (génétique)</term>
<term>Protéines recombinantes (biosynthèse)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Réaction de polymérisation en chaîne (MeSH)</term>
<term>Transformation génétique (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Peroxidases</term>
<term>Recombinant Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term>
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<term>Phanerochaete</term>
<term>Plasmids</term>
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<term>Peroxidases</term>
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<term>Plasmides</term>
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<term>Gene Expression</term>
<term>Molecular Sequence Data</term>
<term>Polymerase Chain Reaction</term>
<term>Transformation, Genetic</term>
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<term>Clonage moléculaire</term>
<term>Données de séquences moléculaires</term>
<term>Expression des gènes</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Transformation génétique</term>
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<front><div type="abstract" xml:lang="en">Two genes, encoding YK-LiP1 and YK-LiP2, were cloned from the white-rot fungus Phanerochaete sordida YK-624, and a homologous expression system for the gene was constructed. Two full-length cDNAs (ylpA and ylpB) were isolated by degenerate RT-PCR and RACE-PCR. The results of N-terminal amino acid sequence analysis of native YK-LiP1 and YK-LiP2 showed that ylpA and ylpB coded for YK-LiP2 and YK-LiP1 respectively. The promoter of glyceraldehyde-3-phosphate dehydrogenase cloned from P. sordida YK-624 (PsGPD) was used to drive the expression of ylpA. Expression vector pGPD-g-ylpA was transformed into a P. sordida YK-624 uracil auxotrophic mutant, UV-64. The YlpA protein was secreted in active form by the transformants after 4 d of growth in a medium containing an excessive nitrogen source, whereas endogenous YK-LiP1 and YK-LiP2 were not produced. The physical and catalytic properties of the purified YlpA protein were very similar to those of YK-LiP2. These results suggest that homologous expression of recombinant YK-LiP2 was successful.</div>
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<Abstract><AbstractText>Two genes, encoding YK-LiP1 and YK-LiP2, were cloned from the white-rot fungus Phanerochaete sordida YK-624, and a homologous expression system for the gene was constructed. Two full-length cDNAs (ylpA and ylpB) were isolated by degenerate RT-PCR and RACE-PCR. The results of N-terminal amino acid sequence analysis of native YK-LiP1 and YK-LiP2 showed that ylpA and ylpB coded for YK-LiP2 and YK-LiP1 respectively. The promoter of glyceraldehyde-3-phosphate dehydrogenase cloned from P. sordida YK-624 (PsGPD) was used to drive the expression of ylpA. Expression vector pGPD-g-ylpA was transformed into a P. sordida YK-624 uracil auxotrophic mutant, UV-64. The YlpA protein was secreted in active form by the transformants after 4 d of growth in a medium containing an excessive nitrogen source, whereas endogenous YK-LiP1 and YK-LiP2 were not produced. The physical and catalytic properties of the purified YlpA protein were very similar to those of YK-LiP2. These results suggest that homologous expression of recombinant YK-LiP2 was successful.</AbstractText>
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Cloning and homologous expression of novel lignin peroxidase genes in the white-rot fungus Phanerochaete sordida YK-624.

Cloning and homologous expression of novel lignin peroxidase genes in the white-rot fungus Phanerochaete sordida YK-624.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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