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Comparative production of ligninolytic enzymes by Phanerochaete chrysosporium and Polyporus sanguineus.

Identifieur interne : 000646 ( Main/Exploration ); précédent : 000645; suivant : 000647

Comparative production of ligninolytic enzymes by Phanerochaete chrysosporium and Polyporus sanguineus.

Auteurs : Paramjit Kaur Bajwa [Canada] ; Daljit Singh Arora

Source :

RBID : pubmed:20029532

Descripteurs français

English descriptors

Abstract

The aim of the present study was to compare the effect of a wide range of culture conditions on production of ligninolytic enzymes by Polyporus sanguineus and Phanerochaete chrysosporium. Lignin peroxidase production by P. sanguineus was comparable with that of P. chrysosporium, although the culture conditions giving the highest yield varied greatly between the two fungi. Highest yield of manganese peroxidase by P. sanguineus obtained in 0.5% malt extract medium and peptone or malt extract supplemented mineral salts broth could not be surpassed by P. chrysosporium in any of the optimization experiments. In addition to lignin peroxidase and manganese peroxidase, P. sanguineus also produced laccase, which was best expressed in malt extract medium supplemented with sugarcane bagasse.

DOI: 10.1139/W09-094
PubMed: 20029532


Affiliations:


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Le document en format XML

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<term>Culture Media (chemistry)</term>
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<term>Laccase (biosynthesis)</term>
<term>Lignin (metabolism)</term>
<term>Peroxidases (biosynthesis)</term>
<term>Phanerochaete (enzymology)</term>
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<term>Laccase (biosynthèse)</term>
<term>Lignine (métabolisme)</term>
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<div type="abstract" xml:lang="en">The aim of the present study was to compare the effect of a wide range of culture conditions on production of ligninolytic enzymes by Polyporus sanguineus and Phanerochaete chrysosporium. Lignin peroxidase production by P. sanguineus was comparable with that of P. chrysosporium, although the culture conditions giving the highest yield varied greatly between the two fungi. Highest yield of manganese peroxidase by P. sanguineus obtained in 0.5% malt extract medium and peptone or malt extract supplemented mineral salts broth could not be surpassed by P. chrysosporium in any of the optimization experiments. In addition to lignin peroxidase and manganese peroxidase, P. sanguineus also produced laccase, which was best expressed in malt extract medium supplemented with sugarcane bagasse.</div>
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