Penetrability of White Rot-Degraded Pine Wood by the Lignin Peroxidase of Phanerochaete chrysosporium.
Identifieur interne : 001003 ( Main/Corpus ); précédent : 001002; suivant : 001004Penetrability of White Rot-Degraded Pine Wood by the Lignin Peroxidase of Phanerochaete chrysosporium.
Auteurs : E. Srebotnik ; K. Messner ; R. FoisnerSource :
- Applied and environmental microbiology [ 0099-2240 ] ; 1988.
Abstract
The penetration of enzymes into wood cell walls during white rot decay is an open question. A postembedding immunoelectron microscopic technique was the method of choice to answer that question. Infiltration of pine wood specimens with a concentrated culture filtrate greatly improved the labeling density and, thereby, reproducibility. Characterization of the concentrated culture filtrate by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting (immunoblotting) revealed three closely spaced proteins of molecular weights about 42,000 showing immunoreactivity against anti-lignin peroxidase serum. It was shown by immunogold labeling that lignin peroxidase of Phanerochaete chrysosporium is located on the surface of the wood cell wall or within areas of heavy attack. It did not diffuse into undecayed parts of the cell wall. The reasons for preventing lignin peroxidase from penetrating wood cell walls during white rot decay are discussed.
DOI: 10.1128/AEM.54.11.2608-2614.1988
PubMed: 16347765
PubMed Central: PMC204343
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Foisner</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1988">1988</date><idno type="RBID">pubmed:16347765</idno><idno type="pmid">16347765</idno><idno type="pmc">PMC204343</idno><idno type="doi">10.1128/AEM.54.11.2608-2614.1988</idno><idno type="wicri:Area/Main/Corpus">001003</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001003</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Penetrability of White Rot-Degraded Pine Wood by the Lignin Peroxidase of Phanerochaete chrysosporium.</title><author><name sortKey="Srebotnik, E" sort="Srebotnik, E" uniqKey="Srebotnik E" first="E" last="Srebotnik">E. Srebotnik</name><affiliation><nlm:affiliation>Institut für Biochemische Technologie und Mikrobiologie, Abteilung Mykologie, Technische Universität Wien, A-1060 Vienna, and Institut für Biochemie, Universität Wien, A-1090 Vienna, Austria.</nlm:affiliation></affiliation></author><author><name sortKey="Messner, K" sort="Messner, K" uniqKey="Messner K" first="K" last="Messner">K. Messner</name></author><author><name sortKey="Foisner, R" sort="Foisner, R" uniqKey="Foisner R" first="R" last="Foisner">R. Foisner</name></author></analytic><series><title level="j">Applied and environmental microbiology</title><idno type="ISSN">0099-2240</idno><imprint><date when="1988" type="published">1988</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The penetration of enzymes into wood cell walls during white rot decay is an open question. A postembedding immunoelectron microscopic technique was the method of choice to answer that question. Infiltration of pine wood specimens with a concentrated culture filtrate greatly improved the labeling density and, thereby, reproducibility. Characterization of the concentrated culture filtrate by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting (immunoblotting) revealed three closely spaced proteins of molecular weights about 42,000 showing immunoreactivity against anti-lignin peroxidase serum. It was shown by immunogold labeling that lignin peroxidase of Phanerochaete chrysosporium is located on the surface of the wood cell wall or within areas of heavy attack. It did not diffuse into undecayed parts of the cell wall. The reasons for preventing lignin peroxidase from penetrating wood cell walls during white rot decay are discussed.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">16347765</PMID><DateCompleted><Year>2010</Year><Month>06</Month><Day>25</Day></DateCompleted><DateRevised><Year>2020</Year><Month>07</Month><Day>27</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0099-2240</ISSN><JournalIssue CitedMedium="Print"><Volume>54</Volume><Issue>11</Issue><PubDate><Year>1988</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Penetrability of White Rot-Degraded Pine Wood by the Lignin Peroxidase of Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>2608-14</MedlinePgn></Pagination><Abstract><AbstractText>The penetration of enzymes into wood cell walls during white rot decay is an open question. A postembedding immunoelectron microscopic technique was the method of choice to answer that question. Infiltration of pine wood specimens with a concentrated culture filtrate greatly improved the labeling density and, thereby, reproducibility. Characterization of the concentrated culture filtrate by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting (immunoblotting) revealed three closely spaced proteins of molecular weights about 42,000 showing immunoreactivity against anti-lignin peroxidase serum. It was shown by immunogold labeling that lignin peroxidase of Phanerochaete chrysosporium is located on the surface of the wood cell wall or within areas of heavy attack. It did not diffuse into undecayed parts of the cell wall. The reasons for preventing lignin peroxidase from penetrating wood cell walls during white rot decay are discussed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Srebotnik</LastName><ForeName>E</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Institut für Biochemische Technologie und Mikrobiologie, Abteilung Mykologie, Technische Universität Wien, A-1060 Vienna, and Institut für Biochemie, Universität Wien, A-1090 Vienna, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Messner</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Foisner</LastName><ForeName>R</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Environ Microbiol</MedlineTA><NlmUniqueID>7605801</NlmUniqueID><ISSNLinking>0099-2240</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1988</Year><Month>11</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1988</Year><Month>11</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1988</Year><Month>11</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16347765</ArticleId><ArticleId IdType="pmc">PMC204343</ArticleId><ArticleId IdType="doi">10.1128/AEM.54.11.2608-2614.1988</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Nature. 1970 Aug 15;227(5259):680-5</Citation><ArticleIdList><ArticleId IdType="pubmed">5432063</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1988 Jan;54(1):62-68</Citation><ArticleIdList><ArticleId IdType="pubmed">16347540</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1984 Sep;48(3):647-53</Citation><ArticleIdList><ArticleId IdType="pubmed">16346631</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1984 Apr;81(8):2280-4</Citation><ArticleIdList><ArticleId IdType="pubmed">16593451</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4</Citation><ArticleIdList><ArticleId IdType="pubmed">388439</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1987 Oct;53(10):2384-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16347459</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Biochem Biophys. 1984 Nov 1;234(2):353-62</Citation><ArticleIdList><ArticleId IdType="pubmed">6497376</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1986 Aug;52(2):239-45</Citation><ArticleIdList><ArticleId IdType="pubmed">16347123</ArticleId></ArticleIdList></Reference><Reference><Citation>Histochemistry. 1984;81(6):573-80</Citation><ArticleIdList><ArticleId IdType="pubmed">6526696</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1985 Mar 10;260(5):2609-12</Citation><ArticleIdList><ArticleId IdType="pubmed">2982828</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Microbiol. 1987;41:465-505</Citation><ArticleIdList><ArticleId IdType="pubmed">3318677</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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