[Research and application of producing laccase by Phanerochaete chrysosporium in solid-state fermentation system].
Identifieur interne : 000639 ( Main/Corpus ); précédent : 000638; suivant : 000640[Research and application of producing laccase by Phanerochaete chrysosporium in solid-state fermentation system].
Auteurs : Dan Peng ; Geng-Xin Xie ; Guang-Ming Zeng ; Yao-Ning Chen ; Fu-Rong Chen ; Shuang Hu ; Zhen YuSource :
- Huan jing ke xue= Huanjing kexue [ 0250-3301 ] ; 2008.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Laccase.
- chemical , metabolism : Laccase.
- chemistry : Musa, Zea mays.
- enzymology : Phanerochaete.
- Fermentation.
Abstract
The potential of banana skin and corn cob as a support-substrate for the production of extracellular laccase by the white-rot fungus Phanerochaete chrysosporium (BKMF-1767) was investigated. The results indicate that laccase showed a maximum activity of 12.68 U/g when the proportion of banana skin and corn cob is 1:2 and the inducer is 0.4 mmol/L CuSO4. In addition, crude laccase enzyme shows degradation activity to pentachlorophenol (PCP) without redox mediator or with the redox mediator (ABTS) at a concentration of 5 mmol/L, and the degradation rates of PCP were 37.8% and 97% respectively after 6 h. The crude laccase was purified by treatment of (NH4)2SO4, and the purified laccase could make the degradation rate of PCP to 81.8% within 6 h.
PubMed: 19256402
Links to Exploration step
pubmed:19256402Le document en format XML
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<author><name sortKey="Peng, Dan" sort="Peng, Dan" uniqKey="Peng D" first="Dan" last="Peng">Dan Peng</name>
<affiliation><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Changsha 410082, China. pengdan987@hotmail.com</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Xie, Geng Xin" sort="Xie, Geng Xin" uniqKey="Xie G" first="Geng-Xin" last="Xie">Geng-Xin Xie</name>
</author>
<author><name sortKey="Zeng, Guang Ming" sort="Zeng, Guang Ming" uniqKey="Zeng G" first="Guang-Ming" last="Zeng">Guang-Ming Zeng</name>
</author>
<author><name sortKey="Chen, Yao Ning" sort="Chen, Yao Ning" uniqKey="Chen Y" first="Yao-Ning" last="Chen">Yao-Ning Chen</name>
</author>
<author><name sortKey="Chen, Fu Rong" sort="Chen, Fu Rong" uniqKey="Chen F" first="Fu-Rong" last="Chen">Fu-Rong Chen</name>
</author>
<author><name sortKey="Hu, Shuang" sort="Hu, Shuang" uniqKey="Hu S" first="Shuang" last="Hu">Shuang Hu</name>
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<author><name sortKey="Yu, Zhen" sort="Yu, Zhen" uniqKey="Yu Z" first="Zhen" last="Yu">Zhen Yu</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Research and application of producing laccase by Phanerochaete chrysosporium in solid-state fermentation system].</title>
<author><name sortKey="Peng, Dan" sort="Peng, Dan" uniqKey="Peng D" first="Dan" last="Peng">Dan Peng</name>
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<author><name sortKey="Zeng, Guang Ming" sort="Zeng, Guang Ming" uniqKey="Zeng G" first="Guang-Ming" last="Zeng">Guang-Ming Zeng</name>
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<author><name sortKey="Chen, Yao Ning" sort="Chen, Yao Ning" uniqKey="Chen Y" first="Yao-Ning" last="Chen">Yao-Ning Chen</name>
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<author><name sortKey="Chen, Fu Rong" sort="Chen, Fu Rong" uniqKey="Chen F" first="Fu-Rong" last="Chen">Fu-Rong Chen</name>
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<author><name sortKey="Hu, Shuang" sort="Hu, Shuang" uniqKey="Hu S" first="Shuang" last="Hu">Shuang Hu</name>
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<author><name sortKey="Yu, Zhen" sort="Yu, Zhen" uniqKey="Yu Z" first="Zhen" last="Yu">Zhen Yu</name>
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<series><title level="j">Huan jing ke xue= Huanjing kexue</title>
<idno type="ISSN">0250-3301</idno>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fermentation (MeSH)</term>
<term>Laccase (biosynthesis)</term>
<term>Laccase (metabolism)</term>
<term>Musa (chemistry)</term>
<term>Phanerochaete (enzymology)</term>
<term>Zea mays (chemistry)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Laccase</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Laccase</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Musa</term>
<term>Zea mays</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term>
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<keywords scheme="MESH" xml:lang="en"><term>Fermentation</term>
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<front><div type="abstract" xml:lang="en">The potential of banana skin and corn cob as a support-substrate for the production of extracellular laccase by the white-rot fungus Phanerochaete chrysosporium (BKMF-1767) was investigated. The results indicate that laccase showed a maximum activity of 12.68 U/g when the proportion of banana skin and corn cob is 1:2 and the inducer is 0.4 mmol/L CuSO4. In addition, crude laccase enzyme shows degradation activity to pentachlorophenol (PCP) without redox mediator or with the redox mediator (ABTS) at a concentration of 5 mmol/L, and the degradation rates of PCP were 37.8% and 97% respectively after 6 h. The crude laccase was purified by treatment of (NH4)2SO4, and the purified laccase could make the degradation rate of PCP to 81.8% within 6 h.</div>
</front>
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<Month>10</Month>
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<DateRevised><Year>2016</Year>
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<Abstract><AbstractText>The potential of banana skin and corn cob as a support-substrate for the production of extracellular laccase by the white-rot fungus Phanerochaete chrysosporium (BKMF-1767) was investigated. The results indicate that laccase showed a maximum activity of 12.68 U/g when the proportion of banana skin and corn cob is 1:2 and the inducer is 0.4 mmol/L CuSO4. In addition, crude laccase enzyme shows degradation activity to pentachlorophenol (PCP) without redox mediator or with the redox mediator (ABTS) at a concentration of 5 mmol/L, and the degradation rates of PCP were 37.8% and 97% respectively after 6 h. The crude laccase was purified by treatment of (NH4)2SO4, and the purified laccase could make the degradation rate of PCP to 81.8% within 6 h.</AbstractText>
</Abstract>
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<Author ValidYN="Y"><LastName>Chen</LastName>
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<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Huan Jing Ke Xue</MedlineTA>
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<ChemicalList><Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D005285" MajorTopicYN="Y">Fermentation</DescriptorName>
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<MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName>
<QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
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<MeshHeading><DescriptorName UI="D028521" MajorTopicYN="N">Musa</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
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<MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName>
<QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName>
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<MeshHeading><DescriptorName UI="D003313" MajorTopicYN="N">Zea mays</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
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