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Kinetic approach for evaluation of total antioxidant activity.

Identifieur interne : 000905 ( PubMed/Corpus ); précédent : 000904; suivant : 000906

Kinetic approach for evaluation of total antioxidant activity.

Auteurs : Elena E. Karyakina ; Darya V. Vokhmyanina ; Natalya V. Sizova ; Aytugan N. Sabitov ; Anastasiya V. Borisova ; Tatyana G. Sazontova ; Yury V. Arkhipenko ; Vsevolod A. Tkachuk ; Yury A. Zolotov ; Arkady A. Karyakin

Source :

RBID : pubmed:19836547

English descriptors

Abstract

We propose a novel approach for assessment of total antioxidant activity by monitoring kinetics of hydrogen peroxide (H(2)O(2)) scavenging after its injection into liquid sample under study. H(2)O(2) is known to be the strongest oxidant, really presented in human body in contrast to the majority of the model oxidative systems used for evaluation of antioxidant activity. In addition, kinetic approach, being more informative than the commonly used determination of the final product, obviously provides better discrimination of potential antioxidants. Prussian Blue based sensor due to its high sensitivity and operational stability allowed to monitor kinetics of hydrogen peroxide consumption in turbid and colored samples. The pseudo-first order kinetic constants of hydrogen peroxide scavenging in the presence of different food additives correlated with total antioxidant activity of these samples evaluated via standard procedure based on lipid peroxidation. However, in contrast to the standard method, the proposed kinetic approach is expressed and does not require fresh biological tissues.

DOI: 10.1016/j.talanta.2009.07.059
PubMed: 19836547

Links to Exploration step

pubmed:19836547

Le document en format XML

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<div type="abstract" xml:lang="en">We propose a novel approach for assessment of total antioxidant activity by monitoring kinetics of hydrogen peroxide (H(2)O(2)) scavenging after its injection into liquid sample under study. H(2)O(2) is known to be the strongest oxidant, really presented in human body in contrast to the majority of the model oxidative systems used for evaluation of antioxidant activity. In addition, kinetic approach, being more informative than the commonly used determination of the final product, obviously provides better discrimination of potential antioxidants. Prussian Blue based sensor due to its high sensitivity and operational stability allowed to monitor kinetics of hydrogen peroxide consumption in turbid and colored samples. The pseudo-first order kinetic constants of hydrogen peroxide scavenging in the presence of different food additives correlated with total antioxidant activity of these samples evaluated via standard procedure based on lipid peroxidation. However, in contrast to the standard method, the proposed kinetic approach is expressed and does not require fresh biological tissues.</div>
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