Simultaneous determination of antioxidants at a chemically modified electrode with vitamin B12 by capillary zone electrophoresis coupled with amperometric detection.
Identifieur interne : 000904 ( PubMed/Corpus ); précédent : 000903; suivant : 000905Simultaneous determination of antioxidants at a chemically modified electrode with vitamin B12 by capillary zone electrophoresis coupled with amperometric detection.
Auteurs : Shuqing Dong ; Langzhu Chi ; Pingang He ; Qingjiang Wang ; Yuzhi FangSource :
- Talanta [ 1873-3573 ] ; 2009.
English descriptors
- KwdEn :
- Antioxidants (analysis), Antioxidants (chemistry), Ascorbic Acid (analysis), Ascorbic Acid (chemistry), Caffeic Acids (analysis), Caffeic Acids (chemistry), Chlorogenic Acid (analysis), Chlorogenic Acid (chemistry), Citrus sinensis (chemistry), Electrochemistry (instrumentation), Electrochemistry (methods), Electrodes, Electrophoresis, Capillary (instrumentation), Electrophoresis, Capillary (methods), Glutathione (analysis), Glutathione (chemistry), Hydrogen-Ion Concentration, Lycopersicon esculentum (chemistry), Oxidation-Reduction, Reproducibility of Results, Salicylates (analysis), Salicylates (chemistry), Vanillic Acid (analysis), Vanillic Acid (chemistry), Vitamin B 12 (chemistry).
- MESH :
- chemical , analysis : Antioxidants, Ascorbic Acid, Caffeic Acids, Chlorogenic Acid, Glutathione, Salicylates, Vanillic Acid.
- chemical , chemistry : Antioxidants, Ascorbic Acid, Caffeic Acids, Chlorogenic Acid, Glutathione, Salicylates, Vanillic Acid, Vitamin B 12.
- chemistry : Citrus sinensis, Lycopersicon esculentum.
- instrumentation : Electrochemistry, Electrophoresis, Capillary.
- methods : Electrochemistry, Electrophoresis, Capillary.
- Electrodes, Hydrogen-Ion Concentration, Oxidation-Reduction, Reproducibility of Results.
Abstract
In the paper, a new kind of vitamin B(12) (acquo-cobalamine) chemically modified electrode was fabricated and applied in capillary zone electrophoresis coupled with amperometric detection (CZE-AD) for simultaneous determination of six antioxidants in fruits and vegetables. The catalytic electrochemical properties of the chemically modified electrode could obviously enhance oxidation peak heights responses by about five times to glutathione, ascorbic acid, vanillic acid, chlorogenic acid, salicylic acid, and caffeic acid compared with common carbon disk electrode. Furthermore, the effects of working electrode potential, pH and concentration of running buffer, separation voltage and injection time on CZE-AD were investigated. Under the optimum conditions, the six analytes could be completely separated and detected in a borate-phosphate buffer (pH 8.4) within 15 min. Their linear ranges were from 2.5x10(-7) to 1.0x10(-4) mol L(-1) and the detection limits were as low as 10(-8) mol L(-1) magnitude (S/N=3). The proposed method has been successfully employed to monitor the six analytes in practical samples with recoveries in the range 96.0-106.0% and RSDs less than 5.0%. Above results demonstrate that capillary zone electrophoresis coupled with electrochemical detection using vitamin B(12) modified electrode as detector is of convenient preparation, high sensitivity, good repeatability, and could be used in the rapid determination of practical samples.
DOI: 10.1016/j.talanta.2009.07.069
PubMed: 19836556
Links to Exploration step
pubmed:19836556Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Simultaneous determination of antioxidants at a chemically modified electrode with vitamin B12 by capillary zone electrophoresis coupled with amperometric detection.</title><author><name sortKey="Dong, Shuqing" sort="Dong, Shuqing" uniqKey="Dong S" first="Shuqing" last="Dong">Shuqing Dong</name><affiliation><nlm:affiliation>Department of Chemistry, East China Normal University, Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chi, Langzhu" sort="Chi, Langzhu" uniqKey="Chi L" first="Langzhu" last="Chi">Langzhu Chi</name></author><author><name sortKey="He, Pingang" sort="He, Pingang" uniqKey="He P" first="Pingang" last="He">Pingang He</name></author><author><name sortKey="Wang, Qingjiang" sort="Wang, Qingjiang" uniqKey="Wang Q" first="Qingjiang" last="Wang">Qingjiang Wang</name></author><author><name sortKey="Fang, Yuzhi" sort="Fang, Yuzhi" uniqKey="Fang Y" first="Yuzhi" last="Fang">Yuzhi Fang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19836556</idno><idno type="pmid">19836556</idno><idno type="doi">10.1016/j.talanta.2009.07.069</idno><idno type="wicri:Area/PubMed/Corpus">000904</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Simultaneous determination of antioxidants at a chemically modified electrode with vitamin B12 by capillary zone electrophoresis coupled with amperometric detection.</title><author><name sortKey="Dong, Shuqing" sort="Dong, Shuqing" uniqKey="Dong S" first="Shuqing" last="Dong">Shuqing Dong</name><affiliation><nlm:affiliation>Department of Chemistry, East China Normal University, Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chi, Langzhu" sort="Chi, Langzhu" uniqKey="Chi L" first="Langzhu" last="Chi">Langzhu Chi</name></author><author><name sortKey="He, Pingang" sort="He, Pingang" uniqKey="He P" first="Pingang" last="He">Pingang He</name></author><author><name sortKey="Wang, Qingjiang" sort="Wang, Qingjiang" uniqKey="Wang Q" first="Qingjiang" last="Wang">Qingjiang Wang</name></author><author><name sortKey="Fang, Yuzhi" sort="Fang, Yuzhi" uniqKey="Fang Y" first="Yuzhi" last="Fang">Yuzhi Fang</name></author></analytic><series><title level="j">Talanta</title><idno type="eISSN">1873-3573</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antioxidants (analysis)</term><term>Antioxidants (chemistry)</term><term>Ascorbic Acid (analysis)</term><term>Ascorbic Acid (chemistry)</term><term>Caffeic Acids (analysis)</term><term>Caffeic Acids (chemistry)</term><term>Chlorogenic Acid (analysis)</term><term>Chlorogenic Acid (chemistry)</term><term>Citrus sinensis (chemistry)</term><term>Electrochemistry (instrumentation)</term><term>Electrochemistry (methods)</term><term>Electrodes</term><term>Electrophoresis, Capillary (instrumentation)</term><term>Electrophoresis, Capillary (methods)</term><term>Glutathione (analysis)</term><term>Glutathione (chemistry)</term><term>Hydrogen-Ion Concentration</term><term>Lycopersicon esculentum (chemistry)</term><term>Oxidation-Reduction</term><term>Reproducibility of Results</term><term>Salicylates (analysis)</term><term>Salicylates (chemistry)</term><term>Vanillic Acid (analysis)</term><term>Vanillic Acid (chemistry)</term><term>Vitamin B 12 (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antioxidants</term><term>Ascorbic Acid</term><term>Caffeic Acids</term><term>Chlorogenic Acid</term><term>Glutathione</term><term>Salicylates</term><term>Vanillic Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antioxidants</term><term>Ascorbic Acid</term><term>Caffeic Acids</term><term>Chlorogenic Acid</term><term>Glutathione</term><term>Salicylates</term><term>Vanillic Acid</term><term>Vitamin B 12</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term><term>Lycopersicon esculentum</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Electrochemistry</term><term>Electrophoresis, Capillary</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrochemistry</term><term>Electrophoresis, Capillary</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electrodes</term><term>Hydrogen-Ion Concentration</term><term>Oxidation-Reduction</term><term>Reproducibility of Results</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the paper, a new kind of vitamin B(12) (acquo-cobalamine) chemically modified electrode was fabricated and applied in capillary zone electrophoresis coupled with amperometric detection (CZE-AD) for simultaneous determination of six antioxidants in fruits and vegetables. The catalytic electrochemical properties of the chemically modified electrode could obviously enhance oxidation peak heights responses by about five times to glutathione, ascorbic acid, vanillic acid, chlorogenic acid, salicylic acid, and caffeic acid compared with common carbon disk electrode. Furthermore, the effects of working electrode potential, pH and concentration of running buffer, separation voltage and injection time on CZE-AD were investigated. Under the optimum conditions, the six analytes could be completely separated and detected in a borate-phosphate buffer (pH 8.4) within 15 min. Their linear ranges were from 2.5x10(-7) to 1.0x10(-4) mol L(-1) and the detection limits were as low as 10(-8) mol L(-1) magnitude (S/N=3). The proposed method has been successfully employed to monitor the six analytes in practical samples with recoveries in the range 96.0-106.0% and RSDs less than 5.0%. Above results demonstrate that capillary zone electrophoresis coupled with electrochemical detection using vitamin B(12) modified electrode as detector is of convenient preparation, high sensitivity, good repeatability, and could be used in the rapid determination of practical samples.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19836556</PMID><DateCreated><Year>2009</Year><Month>10</Month><Day>19</Day></DateCreated><DateCompleted><Year>2010</Year><Month>01</Month><Day>20</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-3573</ISSN><JournalIssue CitedMedium="Internet"><Volume>80</Volume><Issue>2</Issue><PubDate><Year>2009</Year><Month>Dec</Month><Day>15</Day></PubDate></JournalIssue><Title>Talanta</Title><ISOAbbreviation>Talanta</ISOAbbreviation></Journal><ArticleTitle>Simultaneous determination of antioxidants at a chemically modified electrode with vitamin B12 by capillary zone electrophoresis coupled with amperometric detection.</ArticleTitle><Pagination><MedlinePgn>809-14</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.talanta.2009.07.069</ELocationID><Abstract><AbstractText>In the paper, a new kind of vitamin B(12) (acquo-cobalamine) chemically modified electrode was fabricated and applied in capillary zone electrophoresis coupled with amperometric detection (CZE-AD) for simultaneous determination of six antioxidants in fruits and vegetables. The catalytic electrochemical properties of the chemically modified electrode could obviously enhance oxidation peak heights responses by about five times to glutathione, ascorbic acid, vanillic acid, chlorogenic acid, salicylic acid, and caffeic acid compared with common carbon disk electrode. Furthermore, the effects of working electrode potential, pH and concentration of running buffer, separation voltage and injection time on CZE-AD were investigated. Under the optimum conditions, the six analytes could be completely separated and detected in a borate-phosphate buffer (pH 8.4) within 15 min. Their linear ranges were from 2.5x10(-7) to 1.0x10(-4) mol L(-1) and the detection limits were as low as 10(-8) mol L(-1) magnitude (S/N=3). The proposed method has been successfully employed to monitor the six analytes in practical samples with recoveries in the range 96.0-106.0% and RSDs less than 5.0%. Above results demonstrate that capillary zone electrophoresis coupled with electrochemical detection using vitamin B(12) modified electrode as detector is of convenient preparation, high sensitivity, good repeatability, and could be used in the rapid determination of practical samples.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Shuqing</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Chemistry, East China Normal University, Shanghai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chi</LastName><ForeName>Langzhu</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Pingang</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Qingjiang</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Fang</LastName><ForeName>Yuzhi</ForeName><Initials>Y</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>Aug</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Talanta</MedlineTA><NlmUniqueID>2984816R</NlmUniqueID><ISSNLinking>0039-9140</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000975">Antioxidants</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002109">Caffeic Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012459">Salicylates</NameOfSubstance></Chemical><Chemical><RegistryNumber>318ADP12RI</RegistryNumber><NameOfSubstance UI="D002726">Chlorogenic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>GAN16C9B8O</RegistryNumber><NameOfSubstance UI="D005978">Glutathione</NameOfSubstance></Chemical><Chemical><RegistryNumber>GM8Q3JM2Y8</RegistryNumber><NameOfSubstance UI="D014641">Vanillic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>P6YC3EG204</RegistryNumber><NameOfSubstance UI="D014805">Vitamin B 12</NameOfSubstance></Chemical><Chemical><RegistryNumber>PQ6CK8PD0R</RegistryNumber><NameOfSubstance UI="D001205">Ascorbic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>U2S3A33KVM</RegistryNumber><NameOfSubstance UI="C040048">caffeic acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000975" MajorTopicYN="N">Antioxidants</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001205" MajorTopicYN="N">Ascorbic Acid</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002109" MajorTopicYN="N">Caffeic Acids</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002726" MajorTopicYN="N">Chlorogenic Acid</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004563" MajorTopicYN="N">Electrochemistry</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004566" MajorTopicYN="N">Electrodes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019075" MajorTopicYN="N">Electrophoresis, Capillary</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005978" MajorTopicYN="N">Glutathione</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012459" MajorTopicYN="N">Salicylates</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014641" MajorTopicYN="N">Vanillic Acid</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014805" MajorTopicYN="N">Vitamin B 12</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>5</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2009</Year><Month>7</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>7</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>1</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19836556</ArticleId><ArticleId IdType="pii">S0039-9140(09)00643-2</ArticleId><ArticleId IdType="doi">10.1016/j.talanta.2009.07.069</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000904 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000904 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:19836556
|texte= Simultaneous determination of antioxidants at a chemically modified electrode with vitamin B12 by capillary zone electrophoresis coupled with amperometric detection.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:19836556" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |