Comparative iTRAQ proteome and transcriptome analyses of sweet orange infected by "Candidatus Liberibacter asiaticus".
Identifieur interne : 000692 ( PubMed/Corpus ); précédent : 000691; suivant : 000693Comparative iTRAQ proteome and transcriptome analyses of sweet orange infected by "Candidatus Liberibacter asiaticus".
Auteurs : Jing Fan ; Chunxian Chen ; Qibin Yu ; Ronald H. Brlansky ; Zheng-Guo Li ; Frederick G. GmitterSource :
- Physiologia plantarum [ 1399-3054 ] ; 2011.
English descriptors
- KwdEn :
- Chitinase (metabolism), Citrus sinensis (genetics), Copper (metabolism), DNA, Bacterial (analysis), Gene Expression Profiling, Gene Expression Regulation, Plant, Lipoxygenase (metabolism), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Leaves (chemistry), Proteobacteria (physiology), Proteome, Superoxide Dismutase (metabolism), Zinc (metabolism).
- MESH :
- chemical , analysis : DNA, Bacterial.
- chemical , metabolism : Chitinase, Copper, Lipoxygenase, Superoxide Dismutase, Zinc.
- chemistry : Plant Leaves.
- genetics : Citrus sinensis, Plant Diseases.
- microbiology : Plant Diseases.
- physiology : Proteobacteria.
- Gene Expression Profiling, Gene Expression Regulation, Plant, Proteome.
Abstract
Citrus Huanglongbing (HLB) has been threatening citrus production worldwide. In this study, a comparative proteomic approach was applied to understand the pathogenic process of HLB in affected sweet orange leaves. Using the isobaric tags for relative and absolute quantification (iTRAQ) technique, we identified 686 unique proteins in the mature leaves of both mock-inoculated and diseased 'Madam Vinous' sweet orange plants. Of the identified proteins, 20 and 10 were differentially expressed in leaves with and without symptoms of HLB (fold change > 2.5), respectively, compared with mock-inoculated controls. Most significantly, upregulated proteins were involved in stress/defense response, such as four miraculin-like proteins, chitinase, Cu/Zn superoxide dismutase and lipoxygenase. Microarray analysis also showed that stress-related genes were significantly upregulated at the transcriptional level. For example, remarkable upregulations of miraculin-like proteins and Cu/Zn superoxide dismutase transcripts were observed. Moreover, the transcriptional patterns of miraculin-like protein 1 and Cu/Zn superoxide dismutase were examined at different stages of HLB disease development. Combined with the transcriptomic data, the proteomic data can provide an enhanced understanding of citrus stress/defense responses to HLB.
DOI: 10.1111/j.1399-3054.2011.01502.x
PubMed: 21838733
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pubmed:21838733Le document en format XML
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Brlansky</name></author><author><name sortKey="Li, Zheng Guo" sort="Li, Zheng Guo" uniqKey="Li Z" first="Zheng-Guo" last="Li">Zheng-Guo Li</name></author><author><name sortKey="Gmitter, Frederick G" sort="Gmitter, Frederick G" uniqKey="Gmitter F" first="Frederick G" last="Gmitter">Frederick G. Gmitter</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21838733</idno><idno type="pmid">21838733</idno><idno type="doi">10.1111/j.1399-3054.2011.01502.x</idno><idno type="wicri:Area/PubMed/Corpus">000692</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparative iTRAQ proteome and transcriptome analyses of sweet orange infected by "Candidatus Liberibacter asiaticus".</title><author><name sortKey="Fan, Jing" sort="Fan, Jing" uniqKey="Fan J" first="Jing" last="Fan">Jing Fan</name><affiliation><nlm:affiliation>Chongqing University, College of Bioengineering, Department of Bioengineering, Key Laboratory of Biorheological Science and Technology, Ministry of Education, Chongqing 400030, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Chunxian" sort="Chen, Chunxian" uniqKey="Chen C" first="Chunxian" last="Chen">Chunxian Chen</name></author><author><name sortKey="Yu, Qibin" sort="Yu, Qibin" uniqKey="Yu Q" first="Qibin" last="Yu">Qibin Yu</name></author><author><name sortKey="Brlansky, Ronald H" sort="Brlansky, Ronald H" uniqKey="Brlansky R" first="Ronald H" last="Brlansky">Ronald H. Brlansky</name></author><author><name sortKey="Li, Zheng Guo" sort="Li, Zheng Guo" uniqKey="Li Z" first="Zheng-Guo" last="Li">Zheng-Guo Li</name></author><author><name sortKey="Gmitter, Frederick G" sort="Gmitter, Frederick G" uniqKey="Gmitter F" first="Frederick G" last="Gmitter">Frederick G. Gmitter</name></author></analytic><series><title level="j">Physiologia plantarum</title><idno type="eISSN">1399-3054</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chitinase (metabolism)</term><term>Citrus sinensis (genetics)</term><term>Copper (metabolism)</term><term>DNA, Bacterial (analysis)</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Lipoxygenase (metabolism)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (chemistry)</term><term>Proteobacteria (physiology)</term><term>Proteome</term><term>Superoxide Dismutase (metabolism)</term><term>Zinc (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Chitinase</term><term>Copper</term><term>Lipoxygenase</term><term>Superoxide Dismutase</term><term>Zinc</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus sinensis</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Proteobacteria</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Proteome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Citrus Huanglongbing (HLB) has been threatening citrus production worldwide. In this study, a comparative proteomic approach was applied to understand the pathogenic process of HLB in affected sweet orange leaves. Using the isobaric tags for relative and absolute quantification (iTRAQ) technique, we identified 686 unique proteins in the mature leaves of both mock-inoculated and diseased 'Madam Vinous' sweet orange plants. Of the identified proteins, 20 and 10 were differentially expressed in leaves with and without symptoms of HLB (fold change > 2.5), respectively, compared with mock-inoculated controls. Most significantly, upregulated proteins were involved in stress/defense response, such as four miraculin-like proteins, chitinase, Cu/Zn superoxide dismutase and lipoxygenase. Microarray analysis also showed that stress-related genes were significantly upregulated at the transcriptional level. For example, remarkable upregulations of miraculin-like proteins and Cu/Zn superoxide dismutase transcripts were observed. Moreover, the transcriptional patterns of miraculin-like protein 1 and Cu/Zn superoxide dismutase were examined at different stages of HLB disease development. Combined with the transcriptomic data, the proteomic data can provide an enhanced understanding of citrus stress/defense responses to HLB.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21838733</PMID><DateCreated><Year>2011</Year><Month>10</Month><Day>7</Day></DateCreated><DateCompleted><Year>2012</Year><Month>03</Month><Day>01</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1399-3054</ISSN><JournalIssue CitedMedium="Internet"><Volume>143</Volume><Issue>3</Issue><PubDate><Year>2011</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Physiologia plantarum</Title><ISOAbbreviation>Physiol Plant</ISOAbbreviation></Journal><ArticleTitle>Comparative iTRAQ proteome and transcriptome analyses of sweet orange infected by "Candidatus Liberibacter asiaticus".</ArticleTitle><Pagination><MedlinePgn>235-45</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1399-3054.2011.01502.x</ELocationID><Abstract><AbstractText>Citrus Huanglongbing (HLB) has been threatening citrus production worldwide. In this study, a comparative proteomic approach was applied to understand the pathogenic process of HLB in affected sweet orange leaves. Using the isobaric tags for relative and absolute quantification (iTRAQ) technique, we identified 686 unique proteins in the mature leaves of both mock-inoculated and diseased 'Madam Vinous' sweet orange plants. Of the identified proteins, 20 and 10 were differentially expressed in leaves with and without symptoms of HLB (fold change > 2.5), respectively, compared with mock-inoculated controls. Most significantly, upregulated proteins were involved in stress/defense response, such as four miraculin-like proteins, chitinase, Cu/Zn superoxide dismutase and lipoxygenase. Microarray analysis also showed that stress-related genes were significantly upregulated at the transcriptional level. For example, remarkable upregulations of miraculin-like proteins and Cu/Zn superoxide dismutase transcripts were observed. Moreover, the transcriptional patterns of miraculin-like protein 1 and Cu/Zn superoxide dismutase were examined at different stages of HLB disease development. Combined with the transcriptomic data, the proteomic data can provide an enhanced understanding of citrus stress/defense responses to HLB.</AbstractText><CopyrightInformation>Copyright © Physiologia Plantarum 2011.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fan</LastName><ForeName>Jing</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Chongqing University, College of Bioengineering, Department of Bioengineering, Key Laboratory of Biorheological Science and Technology, Ministry of Education, Chongqing 400030, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Chunxian</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Qibin</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Brlansky</LastName><ForeName>Ronald H</ForeName><Initials>RH</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Zheng-Guo</ForeName><Initials>ZG</Initials></Author><Author ValidYN="Y"><LastName>Gmitter</LastName><ForeName>Frederick G</ForeName><Initials>FG</Initials><Suffix>Jr</Suffix></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>Sep</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>Denmark</Country><MedlineTA>Physiol Plant</MedlineTA><NlmUniqueID>1256322</NlmUniqueID><ISSNLinking>0031-9317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical><Chemical><RegistryNumber>789U1901C5</RegistryNumber><NameOfSubstance UI="D003300">Copper</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.13.11.12</RegistryNumber><NameOfSubstance UI="D008084">Lipoxygenase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.15.1.1</RegistryNumber><NameOfSubstance UI="D013482">Superoxide Dismutase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.14</RegistryNumber><NameOfSubstance UI="D002688">Chitinase</NameOfSubstance></Chemical><Chemical><RegistryNumber>J41CSQ7QDS</RegistryNumber><NameOfSubstance UI="D015032">Zinc</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002688" MajorTopicYN="N">Chitinase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003300" MajorTopicYN="N">Copper</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008084" MajorTopicYN="N">Lipoxygenase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020560" MajorTopicYN="N">Proteobacteria</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013482" MajorTopicYN="N">Superoxide Dismutase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015032" MajorTopicYN="N">Zinc</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate 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