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Interspecific somatic hybrid of Ichang papeda with Valencia orange.

Identifieur interne : 000E84 ( PubMed/Checkpoint ); précédent : 000E83; suivant : 000E85

Interspecific somatic hybrid of Ichang papeda with Valencia orange.

Auteurs : X. Deng [République populaire de Chine] ; S. Xiao ; Z. Deng ; W. Zhang

Source :

Chinese journal of biotechnology [ 1042-749X ] ; 1993.

RBID : pubmed:8199318

English descriptors

KwdEn :
- Cells, Cultured, Fruit (genetics), Hybridization, Genetic, Ploidies.
MESH :
- genetics : Fruit.
- Cells, Cultured, Hybridization, Genetic, Ploidies.

Abstract

Protoplasts from the leaf of Ichang papeda (Citrus ichangensis Swingle) were fused with the protoplasts of embryogenic suspension culture of Valencia orange (Citrus sinensis Osbeck) in vitro by polyethylene glycol (PEG)-induced fusion. The regenerated embryoids were malformed and were transferred onto shoot induction medium. The shoots were then grafted on 15-day-old seedlings of trifoliate orange in vitro. Chromosome counts of the young leaves showed that the parents were diploids, 2n = 2x = 18, and the regenerated plants were tetraploids, 2n = 4x = 36. Peroxidase and glutamate oxaloacetate transaminase isozyme analysis confirmed that these tetraploids were somatic hybrids. They have the bands of both parents. The hybrid plants grew vigorously after transplanted into soil. Leaf morphology of the hybrid was similar to that of sweet orange.

PubMed: 8199318

Affiliations:

République populaire de Chine

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Le document en format XML

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<author><name sortKey="Deng, X" sort="Deng, X" uniqKey="Deng X" first="X" last="Deng">X. Deng</name>
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<author><name sortKey="Deng, Z" sort="Deng, Z" uniqKey="Deng Z" first="Z" last="Deng">Z. Deng</name>
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<author><name sortKey="Zhang, W" sort="Zhang, W" uniqKey="Zhang W" first="W" last="Zhang">W. Zhang</name>
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<series><title level="j">Chinese journal of biotechnology</title>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fruit</term>
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<front><div type="abstract" xml:lang="en">Protoplasts from the leaf of Ichang papeda (Citrus ichangensis Swingle) were fused with the protoplasts of embryogenic suspension culture of Valencia orange (Citrus sinensis Osbeck) in vitro by polyethylene glycol (PEG)-induced fusion. The regenerated embryoids were malformed and were transferred onto shoot induction medium. The shoots were then grafted on 15-day-old seedlings of trifoliate orange in vitro. Chromosome counts of the young leaves showed that the parents were diploids, 2n = 2x = 18, and the regenerated plants were tetraploids, 2n = 4x = 36. Peroxidase and glutamate oxaloacetate transaminase isozyme analysis confirmed that these tetraploids were somatic hybrids. They have the bands of both parents. The hybrid plants grew vigorously after transplanted into soil. Leaf morphology of the hybrid was similar to that of sweet orange.</div>
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<Title>Chinese journal of biotechnology</Title>
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<Abstract><AbstractText>Protoplasts from the leaf of Ichang papeda (Citrus ichangensis Swingle) were fused with the protoplasts of embryogenic suspension culture of Valencia orange (Citrus sinensis Osbeck) in vitro by polyethylene glycol (PEG)-induced fusion. The regenerated embryoids were malformed and were transferred onto shoot induction medium. The shoots were then grafted on 15-day-old seedlings of trifoliate orange in vitro. Chromosome counts of the young leaves showed that the parents were diploids, 2n = 2x = 18, and the regenerated plants were tetraploids, 2n = 4x = 36. Peroxidase and glutamate oxaloacetate transaminase isozyme analysis confirmed that these tetraploids were somatic hybrids. They have the bands of both parents. The hybrid plants grew vigorously after transplanted into soil. Leaf morphology of the hybrid was similar to that of sweet orange.</AbstractText>
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Interspecific somatic hybrid of Ichang papeda with Valencia orange.

Interspecific somatic hybrid of Ichang papeda with Valencia orange.

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki