Interspecific somatic hybrid of Ichang papeda with Valencia orange.
Identifieur interne : 000F51 ( PubMed/Curation ); précédent : 000F50; suivant : 000F52Interspecific somatic hybrid of Ichang papeda with Valencia orange.
Auteurs : X. Deng [République populaire de Chine] ; S. Xiao ; Z. Deng ; W. ZhangSource :
- Chinese journal of biotechnology [ 1042-749X ] ; 1993.
English descriptors
- KwdEn :
- MESH :
- genetics : Fruit.
- Cells, Cultured, Hybridization, Genetic, Ploidies.
Abstract
Protoplasts from the leaf of Ichang papeda (Citrus ichangensis Swingle) were fused with the protoplasts of embryogenic suspension culture of Valencia orange (Citrus sinensis Osbeck) in vitro by polyethylene glycol (PEG)-induced fusion. The regenerated embryoids were malformed and were transferred onto shoot induction medium. The shoots were then grafted on 15-day-old seedlings of trifoliate orange in vitro. Chromosome counts of the young leaves showed that the parents were diploids, 2n = 2x = 18, and the regenerated plants were tetraploids, 2n = 4x = 36. Peroxidase and glutamate oxaloacetate transaminase isozyme analysis confirmed that these tetraploids were somatic hybrids. They have the bands of both parents. The hybrid plants grew vigorously after transplanted into soil. Leaf morphology of the hybrid was similar to that of sweet orange.
PubMed: 8199318
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pubmed:8199318Le document en format XML
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<author><name sortKey="Deng, Z" sort="Deng, Z" uniqKey="Deng Z" first="Z" last="Deng">Z. Deng</name>
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<author><name sortKey="Zhang, W" sort="Zhang, W" uniqKey="Zhang W" first="W" last="Zhang">W. Zhang</name>
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<series><title level="j">Chinese journal of biotechnology</title>
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<front><div type="abstract" xml:lang="en">Protoplasts from the leaf of Ichang papeda (Citrus ichangensis Swingle) were fused with the protoplasts of embryogenic suspension culture of Valencia orange (Citrus sinensis Osbeck) in vitro by polyethylene glycol (PEG)-induced fusion. The regenerated embryoids were malformed and were transferred onto shoot induction medium. The shoots were then grafted on 15-day-old seedlings of trifoliate orange in vitro. Chromosome counts of the young leaves showed that the parents were diploids, 2n = 2x = 18, and the regenerated plants were tetraploids, 2n = 4x = 36. Peroxidase and glutamate oxaloacetate transaminase isozyme analysis confirmed that these tetraploids were somatic hybrids. They have the bands of both parents. The hybrid plants grew vigorously after transplanted into soil. Leaf morphology of the hybrid was similar to that of sweet orange.</div>
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<Title>Chinese journal of biotechnology</Title>
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<ArticleTitle>Interspecific somatic hybrid of Ichang papeda with Valencia orange.</ArticleTitle>
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<Abstract><AbstractText>Protoplasts from the leaf of Ichang papeda (Citrus ichangensis Swingle) were fused with the protoplasts of embryogenic suspension culture of Valencia orange (Citrus sinensis Osbeck) in vitro by polyethylene glycol (PEG)-induced fusion. The regenerated embryoids were malformed and were transferred onto shoot induction medium. The shoots were then grafted on 15-day-old seedlings of trifoliate orange in vitro. Chromosome counts of the young leaves showed that the parents were diploids, 2n = 2x = 18, and the regenerated plants were tetraploids, 2n = 4x = 36. Peroxidase and glutamate oxaloacetate transaminase isozyme analysis confirmed that these tetraploids were somatic hybrids. They have the bands of both parents. The hybrid plants grew vigorously after transplanted into soil. Leaf morphology of the hybrid was similar to that of sweet orange.</AbstractText>
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