OrangerV1, PubMed, Checkpoint, bibRecord, 000A38

Analysis of native carotenoid composition in orange juice using C30 columns in tandem.

Identifieur interne : 000A38 ( PubMed/Checkpoint ); précédent : 000A37; suivant : 000A39

Analysis of native carotenoid composition in orange juice using C30 columns in tandem.

Auteurs : Paola Dugo [Italie] ; Miguel Herrero ; Daniele Giuffrida ; Carla Ragonese ; Giacomo Dugo ; Luigi Mondello

Source :

Journal of separation science [ 1615-9314 ] ; 2008.

RBID : pubmed:18615834

English descriptors

KwdEn :
- Beverages (analysis), Carotenoids (analysis), Carotenoids (chemistry), Chromatography, Liquid (methods), Citrus sinensis (chemistry), Mass Spectrometry, Molecular Structure, Saponins, Spectrophotometry, Spectrophotometry, Ultraviolet, Xanthophylls (analysis), Xanthophylls (chemistry).
MESH :
- chemical , analysis : Carotenoids, Xanthophylls.
- analysis : Beverages.
- chemical , chemistry : Carotenoids, Citrus sinensis, Xanthophylls.
- methods : Chromatography, Liquid.
- Mass Spectrometry, Molecular Structure, Saponins, Spectrophotometry, Spectrophotometry, Ultraviolet.

Abstract

In the present contribution, a novel analytical approach based on using serial coupled conventional LC columns is proposed to the study of the native carotenoid composition of orange juice. The great difficulties that are found when analyzing complex carotenoid samples, due to the high natural variability of these compounds as well as to the presence of carotenoid esters are well documented. To overcome some of these limitations, we have developed a methodology including the study of both the saponified and the intact sample by means of two different LC-DAD/APCI-MS methods. The increase in the resolution and separation power obtained when using two serial coupled C(30) columns is demonstrated, and significant increases in peak capacity have been achieved. By using this new methodology, 44 different carotenoids have been tentatively identified. Among them, several violaxanthin diesters have been directly identified in orange juice for the first time. The main carotenoids in orange juice were violaxanthin, lutein, luteoxanthin, 9-cis-antheraxanthin, and beta-cryptoxanthin. Some of them were found in both their free and esterified forms. To the best of our knowledge, this is the first application of serial couplings of C(30) columns for the identification of the native carotenoid composition of natural matrices.

DOI: 10.1002/jssc.200800073
PubMed: 18615834

Affiliations:

Italie

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Le document en format XML

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<author><name sortKey="Dugo, Paola" sort="Dugo, Paola" uniqKey="Dugo P" first="Paola" last="Dugo">Paola Dugo</name>
<affiliation wicri:level="1"><nlm:affiliation>Dipartimento di Scienze degli Alimenti e dell'Ambiente, Facoltà di Scienze MM.FF.NN., Università di Messina, Messina, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Dipartimento di Scienze degli Alimenti e dell'Ambiente, Facoltà di Scienze MM.FF.NN., Università di Messina, Messina</wicri:regionArea>
<wicri:noRegion>Messina</wicri:noRegion>
</affiliation>
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<author><name sortKey="Herrero, Miguel" sort="Herrero, Miguel" uniqKey="Herrero M" first="Miguel" last="Herrero">Miguel Herrero</name>
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<author><name sortKey="Giuffrida, Daniele" sort="Giuffrida, Daniele" uniqKey="Giuffrida D" first="Daniele" last="Giuffrida">Daniele Giuffrida</name>
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<author><name sortKey="Ragonese, Carla" sort="Ragonese, Carla" uniqKey="Ragonese C" first="Carla" last="Ragonese">Carla Ragonese</name>
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<author><name sortKey="Dugo, Giacomo" sort="Dugo, Giacomo" uniqKey="Dugo G" first="Giacomo" last="Dugo">Giacomo Dugo</name>
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<author><name sortKey="Mondello, Luigi" sort="Mondello, Luigi" uniqKey="Mondello L" first="Luigi" last="Mondello">Luigi Mondello</name>
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<author><name sortKey="Herrero, Miguel" sort="Herrero, Miguel" uniqKey="Herrero M" first="Miguel" last="Herrero">Miguel Herrero</name>
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<author><name sortKey="Giuffrida, Daniele" sort="Giuffrida, Daniele" uniqKey="Giuffrida D" first="Daniele" last="Giuffrida">Daniele Giuffrida</name>
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<term>Carotenoids (analysis)</term>
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<term>Chromatography, Liquid (methods)</term>
<term>Citrus sinensis (chemistry)</term>
<term>Mass Spectrometry</term>
<term>Molecular Structure</term>
<term>Saponins</term>
<term>Spectrophotometry</term>
<term>Spectrophotometry, Ultraviolet</term>
<term>Xanthophylls (analysis)</term>
<term>Xanthophylls (chemistry)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Carotenoids</term>
<term>Xanthophylls</term>
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<keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Beverages</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Carotenoids</term>
<term>Citrus sinensis</term>
<term>Xanthophylls</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, Liquid</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Mass Spectrometry</term>
<term>Molecular Structure</term>
<term>Saponins</term>
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<term>Spectrophotometry, Ultraviolet</term>
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<front><div type="abstract" xml:lang="en">In the present contribution, a novel analytical approach based on using serial coupled conventional LC columns is proposed to the study of the native carotenoid composition of orange juice. The great difficulties that are found when analyzing complex carotenoid samples, due to the high natural variability of these compounds as well as to the presence of carotenoid esters are well documented. To overcome some of these limitations, we have developed a methodology including the study of both the saponified and the intact sample by means of two different LC-DAD/APCI-MS methods. The increase in the resolution and separation power obtained when using two serial coupled C(30) columns is demonstrated, and significant increases in peak capacity have been achieved. By using this new methodology, 44 different carotenoids have been tentatively identified. Among them, several violaxanthin diesters have been directly identified in orange juice for the first time. The main carotenoids in orange juice were violaxanthin, lutein, luteoxanthin, 9-cis-antheraxanthin, and beta-cryptoxanthin. Some of them were found in both their free and esterified forms. To the best of our knowledge, this is the first application of serial couplings of C(30) columns for the identification of the native carotenoid composition of natural matrices.</div>
</front>
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<Title>Journal of separation science</Title>
<ISOAbbreviation>J Sep Sci</ISOAbbreviation>
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<ArticleTitle>Analysis of native carotenoid composition in orange juice using C30 columns in tandem.</ArticleTitle>
<Pagination><MedlinePgn>2151-60</MedlinePgn>
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<Abstract><AbstractText>In the present contribution, a novel analytical approach based on using serial coupled conventional LC columns is proposed to the study of the native carotenoid composition of orange juice. The great difficulties that are found when analyzing complex carotenoid samples, due to the high natural variability of these compounds as well as to the presence of carotenoid esters are well documented. To overcome some of these limitations, we have developed a methodology including the study of both the saponified and the intact sample by means of two different LC-DAD/APCI-MS methods. The increase in the resolution and separation power obtained when using two serial coupled C(30) columns is demonstrated, and significant increases in peak capacity have been achieved. By using this new methodology, 44 different carotenoids have been tentatively identified. Among them, several violaxanthin diesters have been directly identified in orange juice for the first time. The main carotenoids in orange juice were violaxanthin, lutein, luteoxanthin, 9-cis-antheraxanthin, and beta-cryptoxanthin. Some of them were found in both their free and esterified forms. To the best of our knowledge, this is the first application of serial couplings of C(30) columns for the identification of the native carotenoid composition of natural matrices.</AbstractText>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dugo</LastName>
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<AffiliationInfo><Affiliation>Dipartimento di Scienze degli Alimenti e dell'Ambiente, Facoltà di Scienze MM.FF.NN., Università di Messina, Messina, Italy.</Affiliation>
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<MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName>
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<MeshHeading><DescriptorName UI="D013056" MajorTopicYN="N">Spectrophotometry, Ultraviolet</DescriptorName>
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<name sortKey="Herrero, Miguel" sort="Herrero, Miguel" uniqKey="Herrero M" first="Miguel" last="Herrero">Miguel Herrero</name>
<name sortKey="Mondello, Luigi" sort="Mondello, Luigi" uniqKey="Mondello L" first="Luigi" last="Mondello">Luigi Mondello</name>
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<country name="Italie"><noRegion><name sortKey="Dugo, Paola" sort="Dugo, Paola" uniqKey="Dugo P" first="Paola" last="Dugo">Paola Dugo</name>
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Analysis of native carotenoid composition in orange juice using C30 columns in tandem.

Analysis of native carotenoid composition in orange juice using C30 columns in tandem.

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki