Assessment of the diagnostic potential of Immmunocapture-PCR and Immuno-PCR for Citrus Variegated Chlorosis.
Identifieur interne : 000A32 ( PubMed/Checkpoint ); précédent : 000A31; suivant : 000A33Assessment of the diagnostic potential of Immmunocapture-PCR and Immuno-PCR for Citrus Variegated Chlorosis.
Auteurs : Luís Antonio Peroni [Brésil] ; José Raimundo Ribeiro Dos Reis ; Helvécio Della Coletta-Filho ; Alessandra Alves De Souza ; Marcos Antonio Machado ; Dagmar Ruth Stach-MachadoSource :
- Journal of microbiological methods [ 0167-7012 ] ; 2008.
English descriptors
- KwdEn :
- Antibodies, Bacterial (immunology), Citrus sinensis (microbiology), Culture Media, Enzyme-Linked Immunosorbent Assay (methods), Plant Diseases (microbiology), Plant Leaves (microbiology), Polymerase Chain Reaction (methods), Sensitivity and Specificity, Xylella (genetics), Xylella (immunology), Xylella (isolation & purification).
- MESH :
- chemical , immunology : Antibodies, Bacterial.
- genetics : Xylella.
- immunology : Xylella.
- isolation & purification : Xylella.
- methods : Enzyme-Linked Immunosorbent Assay, Polymerase Chain Reaction.
- microbiology : Citrus sinensis, Plant Diseases, Plant Leaves.
- chemical : Culture Media, Sensitivity and Specificity.
Abstract
Xylella fastidiosa causes significant losses in many economically important crops. An efficient pathogen detection system is critical for epidemiology studies, particularly when large sample size is involved. In this study we report the development of immunomolecular assays like Immmunocapture-PCR and Immuno-PCR for direct detection of X. fastidiosa without DNA isolation. Whereas the reactivity of ELISA and PCR ranged from 10(6) to 10(4) bacterial cells, the IC-PCR sensitivity was up to 10(3) and the detection limit of I-PCR was up to 10(1) bacterial cells. These methods can use either plant sample extracts or cultivated media, and show no cross reaction for any other endophytic citrus-bacteria. Therefore, IC-PCR and I-PCR assays provide an alternative for quick and very sensitive methods to screening X. fastidiosa, with the advantage of not requiring any concentration or DNA purification steps while still allowing an accurate diagnosis of CVC.
DOI: 10.1016/j.mimet.2008.06.024
PubMed: 18656503
Affiliations:
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last="Reis">José Raimundo Ribeiro Dos Reis</name></author><author><name sortKey="Coletta Filho, Helvecio Della" sort="Coletta Filho, Helvecio Della" uniqKey="Coletta Filho H" first="Helvécio Della" last="Coletta-Filho">Helvécio Della Coletta-Filho</name></author><author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name></author><author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name></author><author><name sortKey="Stach Machado, Dagmar Ruth" sort="Stach Machado, Dagmar Ruth" uniqKey="Stach Machado D" first="Dagmar Ruth" last="Stach-Machado">Dagmar Ruth Stach-Machado</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18656503</idno><idno type="pmid">18656503</idno><idno 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Paulo</region></placeName></affiliation></author><author><name sortKey="Reis, Jose Raimundo Ribeiro Dos" sort="Reis, Jose Raimundo Ribeiro Dos" uniqKey="Reis J" first="José Raimundo Ribeiro Dos" last="Reis">José Raimundo Ribeiro Dos Reis</name></author><author><name sortKey="Coletta Filho, Helvecio Della" sort="Coletta Filho, Helvecio Della" uniqKey="Coletta Filho H" first="Helvécio Della" last="Coletta-Filho">Helvécio Della Coletta-Filho</name></author><author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name></author><author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name></author><author><name sortKey="Stach Machado, Dagmar Ruth" sort="Stach Machado, Dagmar Ruth" uniqKey="Stach Machado D" first="Dagmar Ruth" last="Stach-Machado">Dagmar Ruth Stach-Machado</name></author></analytic><series><title level="j">Journal of microbiological methods</title><idno type="ISSN">0167-7012</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Bacterial (immunology)</term><term>Citrus sinensis (microbiology)</term><term>Culture Media</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (microbiology)</term><term>Polymerase Chain Reaction (methods)</term><term>Sensitivity and Specificity</term><term>Xylella (genetics)</term><term>Xylella (immunology)</term><term>Xylella (isolation & purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Bacterial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Xylella</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Xylella</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Xylella</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus sinensis</term><term>Plant Diseases</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Culture Media</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Xylella fastidiosa causes significant losses in many economically important crops. An efficient pathogen detection system is critical for epidemiology studies, particularly when large sample size is involved. In this study we report the development of immunomolecular assays like Immmunocapture-PCR and Immuno-PCR for direct detection of X. fastidiosa without DNA isolation. Whereas the reactivity of ELISA and PCR ranged from 10(6) to 10(4) bacterial cells, the IC-PCR sensitivity was up to 10(3) and the detection limit of I-PCR was up to 10(1) bacterial cells. These methods can use either plant sample extracts or cultivated media, and show no cross reaction for any other endophytic citrus-bacteria. Therefore, IC-PCR and I-PCR assays provide an alternative for quick and very sensitive methods to screening X. fastidiosa, with the advantage of not requiring any concentration or DNA purification steps while still allowing an accurate diagnosis of CVC.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18656503</PMID><DateCreated><Year>2008</Year><Month>9</Month><Day>8</Day></DateCreated><DateCompleted><Year>2008</Year><Month>11</Month><Day>24</Day></DateCompleted><DateRevised><Year>2008</Year><Month>9</Month><Day>8</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0167-7012</ISSN><JournalIssue CitedMedium="Print"><Volume>75</Volume><Issue>2</Issue><PubDate><Year>2008</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Journal of microbiological methods</Title><ISOAbbreviation>J. Microbiol. Methods</ISOAbbreviation></Journal><ArticleTitle>Assessment of the diagnostic potential of Immmunocapture-PCR and Immuno-PCR for Citrus Variegated Chlorosis.</ArticleTitle><Pagination><MedlinePgn>302-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.mimet.2008.06.024</ELocationID><Abstract><AbstractText>Xylella fastidiosa causes significant losses in many economically important crops. An efficient pathogen detection system is critical for epidemiology studies, particularly when large sample size is involved. In this study we report the development of immunomolecular assays like Immmunocapture-PCR and Immuno-PCR for direct detection of X. fastidiosa without DNA isolation. Whereas the reactivity of ELISA and PCR ranged from 10(6) to 10(4) bacterial cells, the IC-PCR sensitivity was up to 10(3) and the detection limit of I-PCR was up to 10(1) bacterial cells. These methods can use either plant sample extracts or cultivated media, and show no cross reaction for any other endophytic citrus-bacteria. Therefore, IC-PCR and I-PCR assays provide an alternative for quick and very sensitive methods to screening X. fastidiosa, with the advantage of not requiring any concentration or DNA purification steps while still allowing an accurate diagnosis of CVC.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Peroni</LastName><ForeName>Luís Antonio</ForeName><Initials>LA</Initials><AffiliationInfo><Affiliation>Departamento de Microbiologia e Imunologia, Universidade Estadual de Campinas (UNICAMP), Caixa Postal 6109, 13083-970, Campinas, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reis</LastName><ForeName>José Raimundo Ribeiro Dos</ForeName><Initials>JR</Initials></Author><Author ValidYN="Y"><LastName>Coletta-Filho</LastName><ForeName>Helvécio Della</ForeName><Initials>HD</Initials></Author><Author ValidYN="Y"><LastName>de Souza</LastName><ForeName>Alessandra Alves</ForeName><Initials>AA</Initials></Author><Author ValidYN="Y"><LastName>Machado</LastName><ForeName>Marcos Antonio</ForeName><Initials>MA</Initials></Author><Author ValidYN="Y"><LastName>Stach-Machado</LastName><ForeName>Dagmar Ruth</ForeName><Initials>DR</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Studies</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>Jul</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Microbiol Methods</MedlineTA><NlmUniqueID>8306883</NlmUniqueID><ISSNLinking>0167-7012</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000907">Antibodies, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000907" MajorTopicYN="N">Antibodies, Bacterial</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044167" MajorTopicYN="N">Xylella</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2008</Year><Month>3</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2008</Year><Month>6</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2008</Year><Month>6</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>7</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>12</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>7</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18656503</ArticleId><ArticleId IdType="pii">S0167-7012(08)00249-2</ArticleId><ArticleId IdType="doi">10.1016/j.mimet.2008.06.024</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country><region><li>État de São Paulo</li></region></list><tree><noCountry><name sortKey="Coletta Filho, Helvecio Della" sort="Coletta Filho, Helvecio Della" uniqKey="Coletta Filho H" first="Helvécio Della" last="Coletta-Filho">Helvécio Della Coletta-Filho</name><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name><name sortKey="Reis, Jose Raimundo Ribeiro Dos" sort="Reis, Jose Raimundo Ribeiro Dos" uniqKey="Reis J" first="José Raimundo Ribeiro Dos" last="Reis">José Raimundo Ribeiro Dos Reis</name><name sortKey="Stach Machado, Dagmar Ruth" sort="Stach Machado, Dagmar Ruth" uniqKey="Stach Machado D" first="Dagmar Ruth" last="Stach-Machado">Dagmar Ruth Stach-Machado</name></noCountry><country name="Brésil"><region name="État de São Paulo"><name sortKey="Peroni, Luis Antonio" sort="Peroni, Luis Antonio" uniqKey="Peroni L" first="Luís Antonio" last="Peroni">Luís Antonio Peroni</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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