Assessment of the diagnostic potential of Immmunocapture-PCR and Immuno-PCR for Citrus Variegated Chlorosis.
Identifieur interne : 000A22 ( PubMed/Curation ); précédent : 000A21; suivant : 000A23Assessment of the diagnostic potential of Immmunocapture-PCR and Immuno-PCR for Citrus Variegated Chlorosis.
Auteurs : Luís Antonio Peroni [Brésil] ; José Raimundo Ribeiro Dos Reis ; Helvécio Della Coletta-Filho ; Alessandra Alves De Souza ; Marcos Antonio Machado ; Dagmar Ruth Stach-MachadoSource :
- Journal of microbiological methods [ 0167-7012 ] ; 2008.
English descriptors
- KwdEn :
- Antibodies, Bacterial (immunology), Citrus sinensis (microbiology), Culture Media, Enzyme-Linked Immunosorbent Assay (methods), Plant Diseases (microbiology), Plant Leaves (microbiology), Polymerase Chain Reaction (methods), Sensitivity and Specificity, Xylella (genetics), Xylella (immunology), Xylella (isolation & purification).
- MESH :
- chemical , immunology : Antibodies, Bacterial.
- genetics : Xylella.
- immunology : Xylella.
- isolation & purification : Xylella.
- methods : Enzyme-Linked Immunosorbent Assay, Polymerase Chain Reaction.
- microbiology : Citrus sinensis, Plant Diseases, Plant Leaves.
- chemical : Culture Media, Sensitivity and Specificity.
Abstract
Xylella fastidiosa causes significant losses in many economically important crops. An efficient pathogen detection system is critical for epidemiology studies, particularly when large sample size is involved. In this study we report the development of immunomolecular assays like Immmunocapture-PCR and Immuno-PCR for direct detection of X. fastidiosa without DNA isolation. Whereas the reactivity of ELISA and PCR ranged from 10(6) to 10(4) bacterial cells, the IC-PCR sensitivity was up to 10(3) and the detection limit of I-PCR was up to 10(1) bacterial cells. These methods can use either plant sample extracts or cultivated media, and show no cross reaction for any other endophytic citrus-bacteria. Therefore, IC-PCR and I-PCR assays provide an alternative for quick and very sensitive methods to screening X. fastidiosa, with the advantage of not requiring any concentration or DNA purification steps while still allowing an accurate diagnosis of CVC.
DOI: 10.1016/j.mimet.2008.06.024
PubMed: 18656503
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pubmed:18656503Le document en format XML
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<author><name sortKey="Peroni, Luis Antonio" sort="Peroni, Luis Antonio" uniqKey="Peroni L" first="Luís Antonio" last="Peroni">Luís Antonio Peroni</name>
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<author><name sortKey="Reis, Jose Raimundo Ribeiro Dos" sort="Reis, Jose Raimundo Ribeiro Dos" uniqKey="Reis J" first="José Raimundo Ribeiro Dos" last="Reis">José Raimundo Ribeiro Dos Reis</name>
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<author><name sortKey="Stach Machado, Dagmar Ruth" sort="Stach Machado, Dagmar Ruth" uniqKey="Stach Machado D" first="Dagmar Ruth" last="Stach-Machado">Dagmar Ruth Stach-Machado</name>
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<term>Plant Leaves (microbiology)</term>
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<front><div type="abstract" xml:lang="en">Xylella fastidiosa causes significant losses in many economically important crops. An efficient pathogen detection system is critical for epidemiology studies, particularly when large sample size is involved. In this study we report the development of immunomolecular assays like Immmunocapture-PCR and Immuno-PCR for direct detection of X. fastidiosa without DNA isolation. Whereas the reactivity of ELISA and PCR ranged from 10(6) to 10(4) bacterial cells, the IC-PCR sensitivity was up to 10(3) and the detection limit of I-PCR was up to 10(1) bacterial cells. These methods can use either plant sample extracts or cultivated media, and show no cross reaction for any other endophytic citrus-bacteria. Therefore, IC-PCR and I-PCR assays provide an alternative for quick and very sensitive methods to screening X. fastidiosa, with the advantage of not requiring any concentration or DNA purification steps while still allowing an accurate diagnosis of CVC.</div>
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<Abstract><AbstractText>Xylella fastidiosa causes significant losses in many economically important crops. An efficient pathogen detection system is critical for epidemiology studies, particularly when large sample size is involved. In this study we report the development of immunomolecular assays like Immmunocapture-PCR and Immuno-PCR for direct detection of X. fastidiosa without DNA isolation. Whereas the reactivity of ELISA and PCR ranged from 10(6) to 10(4) bacterial cells, the IC-PCR sensitivity was up to 10(3) and the detection limit of I-PCR was up to 10(1) bacterial cells. These methods can use either plant sample extracts or cultivated media, and show no cross reaction for any other endophytic citrus-bacteria. Therefore, IC-PCR and I-PCR assays provide an alternative for quick and very sensitive methods to screening X. fastidiosa, with the advantage of not requiring any concentration or DNA purification steps while still allowing an accurate diagnosis of CVC.</AbstractText>
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