Citrus biotechnology: Achievements, limitations and future directions.
Identifieur interne : 000951 ( PubMed/Checkpoint ); précédent : 000950; suivant : 000952Citrus biotechnology: Achievements, limitations and future directions.
Auteurs : Sandeepa Singh [Inde] ; Manchikatla V. RajamSource :
- Physiology and molecular biology of plants : an international journal of functional plant biology [ 0971-5894 ] ; 2009.
Abstract
Citrus is one of the most important commercial and nutritional fruit crops in the world, hence it needs to be improved to cater to the diverse needs of consumers and crop breeders. Genetic manipulation through conventional techniques in this genus is invariably a difficult task for plant breeders as it poses various biological limitations comprising long juvenile period, high heterozygosity, sexual incompatibility, nucellar polyembryony and large plant size that greatly hinder cultivar improvement. Hence, several attempts were made to improve Citrus sps. by using various in vitro techniques. Citrus sps are widely known for their recalcitrance to transformation and subsequent rooting, but constant research has led to the establishment of improved protocols to ensure the production of uniformly transformed plants, albeit with relatively low efficiency, depending upon the genotype. Genetic modification through Agrobacterium-mediated transformation has emerged as an important tool for introducing agronomically important genes into Citrus sps. Somatic hybridization has been applied to overcome self and cross-incompatibility barriers and generated inter-specific and inter-generic hybrids. Encouraging results have been achieved through transgenics for resistance against viruses and bacteria, thereby augmenting the yield and quality of the fruit. Now, when major transformation and regeneration protocols have sufficiently been standardized for important cultivars, ongoing citrus research focuses mainly on incorporating such genes in citrus genotypes that can combat different biotic and abiotic stresses. This review summarizes the advances made so far in Citrus biotechnology, and suggests some future directions of research in this fruit crop.
DOI: 10.1007/s12298-009-0001-2
PubMed: 23572908
Affiliations:
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Rajam</name></author></analytic><series><title level="j">Physiology and molecular biology of plants : an international journal of functional plant biology</title><idno type="ISSN">0971-5894</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Citrus is one of the most important commercial and nutritional fruit crops in the world, hence it needs to be improved to cater to the diverse needs of consumers and crop breeders. Genetic manipulation through conventional techniques in this genus is invariably a difficult task for plant breeders as it poses various biological limitations comprising long juvenile period, high heterozygosity, sexual incompatibility, nucellar polyembryony and large plant size that greatly hinder cultivar improvement. Hence, several attempts were made to improve Citrus sps. by using various in vitro techniques. Citrus sps are widely known for their recalcitrance to transformation and subsequent rooting, but constant research has led to the establishment of improved protocols to ensure the production of uniformly transformed plants, albeit with relatively low efficiency, depending upon the genotype. Genetic modification through Agrobacterium-mediated transformation has emerged as an important tool for introducing agronomically important genes into Citrus sps. Somatic hybridization has been applied to overcome self and cross-incompatibility barriers and generated inter-specific and inter-generic hybrids. Encouraging results have been achieved through transgenics for resistance against viruses and bacteria, thereby augmenting the yield and quality of the fruit. Now, when major transformation and regeneration protocols have sufficiently been standardized for important cultivars, ongoing citrus research focuses mainly on incorporating such genes in citrus genotypes that can combat different biotic and abiotic stresses. This review summarizes the advances made so far in Citrus biotechnology, and suggests some future directions of research in this fruit crop.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">23572908</PMID><DateCreated><Year>2013</Year><Month>4</Month><Day>10</Day></DateCreated><DateCompleted><Year>2013</Year><Month>04</Month><Day>11</Day></DateCompleted><DateRevised><Year>2013</Year><Month>4</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0971-5894</ISSN><JournalIssue CitedMedium="Print"><Volume>15</Volume><Issue>1</Issue><PubDate><Year>2009</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Physiology and molecular biology of plants : an international journal of functional plant biology</Title><ISOAbbreviation>Physiol Mol Biol Plants</ISOAbbreviation></Journal><ArticleTitle>Citrus biotechnology: Achievements, limitations and future directions.</ArticleTitle><Pagination><MedlinePgn>3-22</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s12298-009-0001-2</ELocationID><Abstract><AbstractText>Citrus is one of the most important commercial and nutritional fruit crops in the world, hence it needs to be improved to cater to the diverse needs of consumers and crop breeders. Genetic manipulation through conventional techniques in this genus is invariably a difficult task for plant breeders as it poses various biological limitations comprising long juvenile period, high heterozygosity, sexual incompatibility, nucellar polyembryony and large plant size that greatly hinder cultivar improvement. Hence, several attempts were made to improve Citrus sps. by using various in vitro techniques. Citrus sps are widely known for their recalcitrance to transformation and subsequent rooting, but constant research has led to the establishment of improved protocols to ensure the production of uniformly transformed plants, albeit with relatively low efficiency, depending upon the genotype. Genetic modification through Agrobacterium-mediated transformation has emerged as an important tool for introducing agronomically important genes into Citrus sps. Somatic hybridization has been applied to overcome self and cross-incompatibility barriers and generated inter-specific and inter-generic hybrids. Encouraging results have been achieved through transgenics for resistance against viruses and bacteria, thereby augmenting the yield and quality of the fruit. Now, when major transformation and regeneration protocols have sufficiently been standardized for important cultivars, ongoing citrus research focuses mainly on incorporating such genes in citrus genotypes that can combat different biotic and abiotic stresses. This review summarizes the advances made so far in Citrus biotechnology, and suggests some future directions of research in this fruit crop.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Singh</LastName><ForeName>Sandeepa</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Genetics, University of Delhi - South Campus, Benito Juarez Road, New Delhi, 110021 India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rajam</LastName><ForeName>Manchikatla V</ForeName><Initials>MV</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>May</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>India</Country><MedlineTA>Physiol Mol Biol Plants</MedlineTA><NlmUniqueID>101304333</NlmUniqueID><ISSNLinking>0974-0430</ISSNLinking></MedlineJournalInfo><OtherID Source="NLM">PMC3550383</OtherID><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Citrus regeneration</Keyword><Keyword MajorTopicYN="N">Citrus sinensis</Keyword><Keyword MajorTopicYN="N">Citrus transformation</Keyword><Keyword MajorTopicYN="N">Citrus tristeza virus</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>4</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23572908</ArticleId><ArticleId IdType="doi">10.1007/s12298-009-0001-2</ArticleId><ArticleId IdType="pii">1</ArticleId><ArticleId IdType="pmc">PMC3550383</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Rajam, Manchikatla V" sort="Rajam, Manchikatla V" uniqKey="Rajam M" first="Manchikatla V" last="Rajam">Manchikatla V. Rajam</name></noCountry><country name="Inde"><noRegion><name sortKey="Singh, Sandeepa" sort="Singh, Sandeepa" uniqKey="Singh S" first="Sandeepa" last="Singh">Sandeepa Singh</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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