Ethanol production from orange peels: two-stage hydrolysis and fermentation studies using optimized parameters through experimental design.
Identifieur interne : 000819 ( PubMed/Checkpoint ); précédent : 000818; suivant : 000820Ethanol production from orange peels: two-stage hydrolysis and fermentation studies using optimized parameters through experimental design.
Auteurs : Harinder Singh Oberoi [États-Unis] ; Praveen Venkata Vadlani ; Ronald L. Madl ; Lavudi Saida ; Jithma P. AbeykoonSource :
- Journal of agricultural and food chemistry [ 1520-5118 ] ; 2010.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Ethanol.
- chemistry : Citrus sinensis.
- metabolism : Citrus sinensis, Yeasts.
- methods : Biotechnology.
- Fermentation, Hydrolysis.
Abstract
Orange peels were evaluated as a fermentation feedstock, and process conditions for enhanced ethanol production were determined. Primary hydrolysis of orange peel powder (OPP) was carried out at acid concentrations from 0 to 1.0% (w/v) at 121 degrees C and 15 psi for 15 min. High-performance liquid chromatography analysis of sugars and inhibitory compounds showed a higher production of hydroxymethyfurfural and acetic acid and a decrease in sugar concentration when the acid level was beyond 0.5% (w/v). Secondary hydrolysis of pretreated biomass obtained from primary hydrolysis was carried out at 0.5% (w/v) acid. Response surface methodology using three factors and a two-level central composite design was employed to optimize the effect of pH, temperature, and fermentation time on ethanol production from OPP hydrolysate at the shake flask level. On the basis of results obtained from the optimization experiment and numerical optimization software, a validation study was carried out in a 2 L batch fermenter at pH 5.4 and a temperature of 34 degrees C for 15 h. The hydrolysate obtained from primary and secondary hydrolysis processes was fermented separately employing parameters optimized through RSM. Ethanol yields of 0.25 g/g on a biomass basis (YP/X) and 0.46 g/g on a substrate-consumed basis (YP/S) and a promising volumetric ethanol productivity of 3.37 g/L/h were attained using this process at the fermenter level, which shows promise for further scale-up studies.
DOI: 10.1021/jf903163t
PubMed: 20158208
Affiliations:
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Madl</name></author><author><name sortKey="Saida, Lavudi" sort="Saida, Lavudi" uniqKey="Saida L" first="Lavudi" last="Saida">Lavudi Saida</name></author><author><name sortKey="Abeykoon, Jithma P" sort="Abeykoon, Jithma P" uniqKey="Abeykoon J" first="Jithma P" last="Abeykoon">Jithma P. 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Madl</name></author><author><name sortKey="Saida, Lavudi" sort="Saida, Lavudi" uniqKey="Saida L" first="Lavudi" last="Saida">Lavudi Saida</name></author><author><name sortKey="Abeykoon, Jithma P" sort="Abeykoon, Jithma P" uniqKey="Abeykoon J" first="Jithma P" last="Abeykoon">Jithma P. Abeykoon</name></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="eISSN">1520-5118</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biotechnology (methods)</term><term>Citrus sinensis (chemistry)</term><term>Citrus sinensis (metabolism)</term><term>Ethanol (metabolism)</term><term>Fermentation</term><term>Hydrolysis</term><term>Yeasts (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ethanol</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus sinensis</term><term>Yeasts</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biotechnology</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Fermentation</term><term>Hydrolysis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Orange peels were evaluated as a fermentation feedstock, and process conditions for enhanced ethanol production were determined. Primary hydrolysis of orange peel powder (OPP) was carried out at acid concentrations from 0 to 1.0% (w/v) at 121 degrees C and 15 psi for 15 min. High-performance liquid chromatography analysis of sugars and inhibitory compounds showed a higher production of hydroxymethyfurfural and acetic acid and a decrease in sugar concentration when the acid level was beyond 0.5% (w/v). Secondary hydrolysis of pretreated biomass obtained from primary hydrolysis was carried out at 0.5% (w/v) acid. Response surface methodology using three factors and a two-level central composite design was employed to optimize the effect of pH, temperature, and fermentation time on ethanol production from OPP hydrolysate at the shake flask level. On the basis of results obtained from the optimization experiment and numerical optimization software, a validation study was carried out in a 2 L batch fermenter at pH 5.4 and a temperature of 34 degrees C for 15 h. The hydrolysate obtained from primary and secondary hydrolysis processes was fermented separately employing parameters optimized through RSM. Ethanol yields of 0.25 g/g on a biomass basis (YP/X) and 0.46 g/g on a substrate-consumed basis (YP/S) and a promising volumetric ethanol productivity of 3.37 g/L/h were attained using this process at the fermenter level, which shows promise for further scale-up studies.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20158208</PMID><DateCreated><Year>2010</Year><Month>3</Month><Day>17</Day></DateCreated><DateCompleted><Year>2010</Year><Month>07</Month><Day>15</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1520-5118</ISSN><JournalIssue CitedMedium="Internet"><Volume>58</Volume><Issue>6</Issue><PubDate><Year>2010</Year><Month>Mar</Month><Day>24</Day></PubDate></JournalIssue><Title>Journal of agricultural and food chemistry</Title><ISOAbbreviation>J. Agric. Food Chem.</ISOAbbreviation></Journal><ArticleTitle>Ethanol production from orange peels: two-stage hydrolysis and fermentation studies using optimized parameters through experimental design.</ArticleTitle><Pagination><MedlinePgn>3422-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/jf903163t</ELocationID><Abstract><AbstractText>Orange peels were evaluated as a fermentation feedstock, and process conditions for enhanced ethanol production were determined. Primary hydrolysis of orange peel powder (OPP) was carried out at acid concentrations from 0 to 1.0% (w/v) at 121 degrees C and 15 psi for 15 min. High-performance liquid chromatography analysis of sugars and inhibitory compounds showed a higher production of hydroxymethyfurfural and acetic acid and a decrease in sugar concentration when the acid level was beyond 0.5% (w/v). Secondary hydrolysis of pretreated biomass obtained from primary hydrolysis was carried out at 0.5% (w/v) acid. Response surface methodology using three factors and a two-level central composite design was employed to optimize the effect of pH, temperature, and fermentation time on ethanol production from OPP hydrolysate at the shake flask level. On the basis of results obtained from the optimization experiment and numerical optimization software, a validation study was carried out in a 2 L batch fermenter at pH 5.4 and a temperature of 34 degrees C for 15 h. The hydrolysate obtained from primary and secondary hydrolysis processes was fermented separately employing parameters optimized through RSM. Ethanol yields of 0.25 g/g on a biomass basis (YP/X) and 0.46 g/g on a substrate-consumed basis (YP/S) and a promising volumetric ethanol productivity of 3.37 g/L/h were attained using this process at the fermenter level, which shows promise for further scale-up studies.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Oberoi</LastName><ForeName>Harinder Singh</ForeName><Initials>HS</Initials><AffiliationInfo><Affiliation>Department of Grain Science and Industry, Kansas State University, Manhattan, Kansas 66506, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vadlani</LastName><ForeName>Praveen Venkata</ForeName><Initials>PV</Initials></Author><Author ValidYN="Y"><LastName>Madl</LastName><ForeName>Ronald L</ForeName><Initials>RL</Initials></Author><Author ValidYN="Y"><LastName>Saida</LastName><ForeName>Lavudi</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Abeykoon</LastName><ForeName>Jithma P</ForeName><Initials>JP</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Studies</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Agric Food Chem</MedlineTA><NlmUniqueID>0374755</NlmUniqueID><ISSNLinking>0021-8561</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>3K9958V90M</RegistryNumber><NameOfSubstance UI="D000431">Ethanol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001709" MajorTopicYN="N">Biotechnology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000431" MajorTopicYN="N">Ethanol</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005285" MajorTopicYN="Y">Fermentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015003" MajorTopicYN="N">Yeasts</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>2</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>2</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>7</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20158208</ArticleId><ArticleId IdType="doi">10.1021/jf903163t</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Abeykoon, Jithma P" sort="Abeykoon, Jithma P" uniqKey="Abeykoon J" first="Jithma P" last="Abeykoon">Jithma P. Abeykoon</name><name sortKey="Madl, Ronald L" sort="Madl, Ronald L" uniqKey="Madl R" first="Ronald L" last="Madl">Ronald L. Madl</name><name sortKey="Saida, Lavudi" sort="Saida, Lavudi" uniqKey="Saida L" first="Lavudi" last="Saida">Lavudi Saida</name><name sortKey="Vadlani, Praveen Venkata" sort="Vadlani, Praveen Venkata" uniqKey="Vadlani P" first="Praveen Venkata" last="Vadlani">Praveen Venkata Vadlani</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Oberoi, Harinder Singh" sort="Oberoi, Harinder Singh" uniqKey="Oberoi H" first="Harinder Singh" last="Oberoi">Harinder Singh Oberoi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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