Antimycotic Activity and Genotoxic Evaluation of Citrus sinensis and Citrus latifolia Essential Oils.
Identifieur interne : 000085 ( PubMed/Checkpoint ); précédent : 000084; suivant : 000086Antimycotic Activity and Genotoxic Evaluation of Citrus sinensis and Citrus latifolia Essential Oils.
Auteurs : Nancy J. Ruiz-Pérez ; Marisela González-Ávila ; Jaime Sánchez-Navarrete ; Julia D. Toscano-Garibay ; Mario A. Moreno-Eutimio ; Teresa Sandoval-Hernández ; Myriam Arriaga-AlbaSource :
- Scientific reports [ 2045-2322 ] ; 2016.
Abstract
The aim of this study was to evaluate the antifungal activity of essential oils (EOs) of Citrus sinensis (C. sinensis) and Citrus latifolia (C. latifolia) against five Candida species: Candida albicans, Candida tropicalis, Candida glabrata, Candida lusitaniae and Candida guilliermondii; and perform its genotoxic evaluation. The EOs of C. sinensis and C. latifolia were obtained from the peel by hydro-distillation. The major components determined by GC-MS were in C. sinensis, d-limonene (96%) and α-myrcene (2.79%); and in C. latifolia, d-limonene (51.64%), β-thujene (14.85%), β-pinene (12.79%) and γ-terpinene (12.8%). Antifungal properties were studied by agar diffusion method, where C. sinensis presented low activity and C. latifolia essential oil was effective to inhibit growing of C. lusitaniae and C. guilliermondii with IC50 of 6.90 and 2.92 μg respectively. The minimum inhibitory concentrations (MIC) for C. sinensis were in a range of 0.42-3.71 μg and for C. latifolia of 0.22-1.30 μg. Genotoxic evaluation was done by Ames test where none of the oils induced point mutations. Flow cytometry was used to measure toxicity in human oral epithelial cells, C. sinensis was not cytotoxic and C. latifolia was toxic at 21.8 μg. These properties might bestow different odontological applications to each essential oil.
DOI: 10.1038/srep25371
PubMed: 27137128
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Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation><wicri:noCountry code="subField">México D.F.</wicri:noCountry></affiliation></author><author><name sortKey="Gonzalez Vila, Marisela" sort="Gonzalez Vila, Marisela" uniqKey="Gonzalez Vila M" first="Marisela" last="González-Ávila">Marisela González-Ávila</name><affiliation><nlm:affiliation>Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. Av. Normalistas # 800 Col. Colinas de la Normal, Guadalajara, Jal. México C.P. 44270.</nlm:affiliation><wicri:noCountry code="subField">Jal</wicri:noCountry></affiliation></author><author><name sortKey="Sanchez Navarrete, Jaime" sort="Sanchez Navarrete, Jaime" uniqKey="Sanchez Navarrete J" first="Jaime" last="Sánchez-Navarrete">Jaime Sánchez-Navarrete</name><affiliation><nlm:affiliation>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation><wicri:noCountry code="subField">México D.F.</wicri:noCountry></affiliation></author><author><name sortKey="Toscano Garibay, Julia D" sort="Toscano Garibay, Julia D" uniqKey="Toscano Garibay J" first="Julia D" last="Toscano-Garibay">Julia D. Toscano-Garibay</name><affiliation><nlm:affiliation>Laboratorio de Medicina Regenerativa, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation><wicri:noCountry code="subField">México D.F.</wicri:noCountry></affiliation></author><author><name sortKey="Moreno Eutimio, Mario A" sort="Moreno Eutimio, Mario A" uniqKey="Moreno Eutimio M" first="Mario A" last="Moreno-Eutimio">Mario A. Moreno-Eutimio</name><affiliation><nlm:affiliation>Laboratorio de Inmunobiología, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation><wicri:noCountry code="subField">México D.F.</wicri:noCountry></affiliation></author><author><name sortKey="Sandoval Hernandez, Teresa" sort="Sandoval Hernandez, Teresa" uniqKey="Sandoval Hernandez T" first="Teresa" last="Sandoval-Hernández">Teresa Sandoval-Hernández</name><affiliation><nlm:affiliation>Laboratorio de Inmunobiología, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation><wicri:noCountry code="subField">México D.F.</wicri:noCountry></affiliation></author><author><name sortKey="Arriaga Alba, Myriam" sort="Arriaga Alba, Myriam" uniqKey="Arriaga Alba M" first="Myriam" last="Arriaga-Alba">Myriam Arriaga-Alba</name><affiliation><nlm:affiliation>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</nlm:affiliation><wicri:noCountry code="subField">México D.F.</wicri:noCountry></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this study was to evaluate the antifungal activity of essential oils (EOs) of Citrus sinensis (C. sinensis) and Citrus latifolia (C. latifolia) against five Candida species: Candida albicans, Candida tropicalis, Candida glabrata, Candida lusitaniae and Candida guilliermondii; and perform its genotoxic evaluation. The EOs of C. sinensis and C. latifolia were obtained from the peel by hydro-distillation. The major components determined by GC-MS were in C. sinensis, d-limonene (96%) and α-myrcene (2.79%); and in C. latifolia, d-limonene (51.64%), β-thujene (14.85%), β-pinene (12.79%) and γ-terpinene (12.8%). Antifungal properties were studied by agar diffusion method, where C. sinensis presented low activity and C. latifolia essential oil was effective to inhibit growing of C. lusitaniae and C. guilliermondii with IC50 of 6.90 and 2.92 μg respectively. The minimum inhibitory concentrations (MIC) for C. sinensis were in a range of 0.42-3.71 μg and for C. latifolia of 0.22-1.30 μg. Genotoxic evaluation was done by Ames test where none of the oils induced point mutations. Flow cytometry was used to measure toxicity in human oral epithelial cells, C. sinensis was not cytotoxic and C. latifolia was toxic at 21.8 μg. These properties might bestow different odontological applications to each essential oil.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">27137128</PMID><DateCreated><Year>2016</Year><Month>5</Month><Day>4</Day></DateCreated><DateRevised><Year>2016</Year><Month>5</Month><Day>17</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>6</Volume><PubDate><Year>2016</Year><Month>May</Month><Day>03</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>Antimycotic Activity and Genotoxic Evaluation of Citrus sinensis and Citrus latifolia Essential Oils.</ArticleTitle><Pagination><MedlinePgn>25371</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/srep25371</ELocationID><Abstract><AbstractText>The aim of this study was to evaluate the antifungal activity of essential oils (EOs) of Citrus sinensis (C. sinensis) and Citrus latifolia (C. latifolia) against five Candida species: Candida albicans, Candida tropicalis, Candida glabrata, Candida lusitaniae and Candida guilliermondii; and perform its genotoxic evaluation. The EOs of C. sinensis and C. latifolia were obtained from the peel by hydro-distillation. The major components determined by GC-MS were in C. sinensis, d-limonene (96%) and α-myrcene (2.79%); and in C. latifolia, d-limonene (51.64%), β-thujene (14.85%), β-pinene (12.79%) and γ-terpinene (12.8%). Antifungal properties were studied by agar diffusion method, where C. sinensis presented low activity and C. latifolia essential oil was effective to inhibit growing of C. lusitaniae and C. guilliermondii with IC50 of 6.90 and 2.92 μg respectively. The minimum inhibitory concentrations (MIC) for C. sinensis were in a range of 0.42-3.71 μg and for C. latifolia of 0.22-1.30 μg. Genotoxic evaluation was done by Ames test where none of the oils induced point mutations. Flow cytometry was used to measure toxicity in human oral epithelial cells, C. sinensis was not cytotoxic and C. latifolia was toxic at 21.8 μg. These properties might bestow different odontological applications to each essential oil.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ruiz-Pérez</LastName><ForeName>Nancy J</ForeName><Initials>NJ</Initials><AffiliationInfo><Affiliation>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>González-Ávila</LastName><ForeName>Marisela</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. Av. Normalistas # 800 Col. Colinas de la Normal, Guadalajara, Jal. México C.P. 44270.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sánchez-Navarrete</LastName><ForeName>Jaime</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Toscano-Garibay</LastName><ForeName>Julia D</ForeName><Initials>JD</Initials><AffiliationInfo><Affiliation>Laboratorio de Medicina Regenerativa, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moreno-Eutimio</LastName><ForeName>Mario A</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Laboratorio de Inmunobiología, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sandoval-Hernández</LastName><ForeName>Teresa</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Laboratorio de Inmunobiología, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arriaga-Alba</LastName><ForeName>Myriam</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratorio de Investigación microbiológica, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, México D.F. C.P. 07650.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>May</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>J Appl Microbiol. 1999 Jun;86(6):985-90</RefSource><PMID Version="1">10438227</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Phytother Res. 1999 Aug;13(5):397-400</RefSource><PMID Version="1">10441779</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Clin Microbiol Rev. 1999 Oct;12(4):501-17</RefSource><PMID Version="1">10515900</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Forsch Komplementmed. 2009 Apr;16(2):79-90</RefSource><PMID Version="1">19420953</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Food Chem Toxicol. 2010 Jun;48(6):1734-40</RefSource><PMID Version="1">20385194</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Molecules. 2010 Nov;15(11):7532-46</RefSource><PMID Version="1">21030907</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Roum Arch Microbiol Immunol. 2010 Jul-Sep;69(3):145-52</RefSource><PMID Version="1">21434591</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Food Chem Toxicol. 2013 Mar;53:228-34</RefSource><PMID Version="1">23201371</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Drugs. 2013 Jun;73(9):919-34</RefSource><PMID Version="1">23729001</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Sci Rep. 2015;5:9657</RefSource><PMID Version="1">25965506</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Agric Food Chem. 2002 Feb 13;50(4):796-805</RefSource><PMID Version="1">11829647</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>J Appl Microbiol. 2003;94(4):665-74</RefSource><PMID Version="1">12631202</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Free Radic Res. 2003 Jun;37(6):681-7</RefSource><PMID Version="1">12868495</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Toxicol Lett. 2004 Aug 1;151(3):481-7</RefSource><PMID Version="1">15261992</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Arch Biochem Biophys. 1974 Jan;160(1):83-9</RefSource><PMID Version="1">4597559</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Mutat Res. 1983 May;113(3-4):173-215</RefSource><PMID Version="1">6341825</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Science. 1987 Apr 17;236(4799):271-80</RefSource><PMID Version="1">3563506</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Pharmazie. 1994 Jun;49(6):448-51</RefSource><PMID Version="1">8047546</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>New Horiz. 1996 Aug;4(3):338-44</RefSource><PMID Version="1">8856751</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Teratog Carcinog Mutagen. 1996;16(2):125-38</RefSource><PMID Version="1">8875742</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Int J Food Microbiol. 1996 Nov;33(1):85-102</RefSource><PMID Version="1">8913811</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Int J Food Microbiol. 1998 Aug 18;43(1-2):73-9</RefSource><PMID Version="1">9761340</PMID></CommentsCorrections></CommentsCorrectionsList><OtherID Source="NLM">PMC4853807</OtherID></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>9</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>4</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>5</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>5</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>5</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27137128</ArticleId><ArticleId IdType="pii">srep25371</ArticleId><ArticleId IdType="doi">10.1038/srep25371</ArticleId><ArticleId IdType="pmc">PMC4853807</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Arriaga Alba, Myriam" sort="Arriaga Alba, Myriam" uniqKey="Arriaga Alba M" first="Myriam" last="Arriaga-Alba">Myriam Arriaga-Alba</name><name sortKey="Gonzalez Vila, Marisela" sort="Gonzalez Vila, Marisela" uniqKey="Gonzalez Vila M" first="Marisela" last="González-Ávila">Marisela González-Ávila</name><name sortKey="Moreno Eutimio, Mario A" sort="Moreno Eutimio, Mario A" uniqKey="Moreno Eutimio M" first="Mario A" last="Moreno-Eutimio">Mario A. Moreno-Eutimio</name><name sortKey="Ruiz Perez, Nancy J" sort="Ruiz Perez, Nancy J" uniqKey="Ruiz Perez N" first="Nancy J" last="Ruiz-Pérez">Nancy J. Ruiz-Pérez</name><name sortKey="Sanchez Navarrete, Jaime" sort="Sanchez Navarrete, Jaime" uniqKey="Sanchez Navarrete J" first="Jaime" last="Sánchez-Navarrete">Jaime Sánchez-Navarrete</name><name sortKey="Sandoval Hernandez, Teresa" sort="Sandoval Hernandez, Teresa" uniqKey="Sandoval Hernandez T" first="Teresa" last="Sandoval-Hernández">Teresa Sandoval-Hernández</name><name sortKey="Toscano Garibay, Julia D" sort="Toscano Garibay, Julia D" uniqKey="Toscano Garibay J" first="Julia D" last="Toscano-Garibay">Julia D. Toscano-Garibay</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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