Synergistic anti-inflammatory effects of Nobiletin and Sulforaphane in lipopolysaccharide-stimulated RAW 264.7 cells
Identifieur interne : 000A81 ( Pmc/Curation ); précédent : 000A80; suivant : 000A82Synergistic anti-inflammatory effects of Nobiletin and Sulforaphane in lipopolysaccharide-stimulated RAW 264.7 cells
Auteurs : Shanshan Guo [République populaire de Chine, États-Unis] ; Peiju Qiu [États-Unis] ; Guang Xu [États-Unis] ; Xian Wu [États-Unis] ; Ping Dong [États-Unis] ; Guanpin Yang [République populaire de Chine] ; Jinkai Zheng [États-Unis] ; David Julian Mcclements [États-Unis] ; Hang Xiao [États-Unis]Source :
- Journal of Agricultural and Food Chemistry [ 0021-8561 ] ; 2012.
Abstract
Inflammation plays important roles in initiation and progress of many diseases including cancers in multiple organ sites. Herein, we investigated the anti-inflammatory effects of two dietary compounds, nobiletin (NBN) and sulforaphane (SFN) in combination. Non-cytotoxic concentrations of NBN, SFN, and their combinations were studied in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. The results showed that combined NBN and SFN treatments produced much stronger inhibitory effects on the production of nitric oxide (NO) than NBN or SFN alone at higher concentrations. These enhanced inhibitory effects were synergistic based on the isobologram analysis. Western blot analysis showed that combined NBN and SFN treatments synergistically decreased iNOS and COX-2 protein expression levels and induced heme oxygenase-1 (HO-1) protein expression. Real-time PCR analysis indicated that low doses of NBN and SFN in combination significantly suppressed LPS-induced upregulation of IL-1 mRNA levels, and synergistically increased HO-1 mRNA levels. Overall our results demonstrated that NBN and SFN in combination produced synergistic effects in inhibiting LPS-induced inflammation in RAW 264.7 cells.
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DOI: 10.1021/jf300129t
PubMed: 22335189
PubMed Central: 3296826
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R. China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Marine Life Science, Ocean University of China, Qingdao, Shandong</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="A2">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Food Science, University of Massachusetts, Amherst, MA</wicri:regionArea></affiliation></author><author><name sortKey="Qiu, Peiju" sort="Qiu, Peiju" uniqKey="Qiu P" first="Peiju" last="Qiu">Peiju Qiu</name><affiliation wicri:level="1"><nlm:aff id="A2">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Food Science, University of Massachusetts, Amherst, MA</wicri:regionArea></affiliation></author><author><name sortKey="Xu, Guang" sort="Xu, Guang" uniqKey="Xu G" first="Guang" last="Xu">Guang Xu</name><affiliation wicri:level="1"><nlm:aff id="A3">Department of Plant, Soil & Insect Sciences, University of Massachusetts, Amherst, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant, Soil & Insect Sciences, University of Massachusetts, Amherst, MA</wicri:regionArea></affiliation></author><author><name sortKey="Wu, Xian" sort="Wu, Xian" uniqKey="Wu X" first="Xian" last="Wu">Xian Wu</name><affiliation wicri:level="1"><nlm:aff id="A2">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Food Science, University of Massachusetts, Amherst, MA</wicri:regionArea></affiliation></author><author><name sortKey="Dong, Ping" sort="Dong, Ping" uniqKey="Dong P" first="Ping" last="Dong">Ping Dong</name><affiliation wicri:level="1"><nlm:aff id="A2">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Food Science, University of Massachusetts, Amherst, MA</wicri:regionArea></affiliation></author><author><name sortKey="Yang, Guanpin" sort="Yang, Guanpin" uniqKey="Yang G" first="Guanpin" last="Yang">Guanpin Yang</name><affiliation wicri:level="1"><nlm:aff id="A1">College of Marine Life Science, Ocean University of China, Qingdao, Shandong, P. 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China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Marine Life Science, Ocean University of China, Qingdao, Shandong</wicri:regionArea></affiliation></author><author><name sortKey="Zheng, Jinkai" sort="Zheng, Jinkai" uniqKey="Zheng J" first="Jinkai" last="Zheng">Jinkai Zheng</name><affiliation wicri:level="1"><nlm:aff id="A2">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Food Science, University of Massachusetts, Amherst, MA</wicri:regionArea></affiliation></author><author><name sortKey="Mcclements, David Julian" sort="Mcclements, David Julian" uniqKey="Mcclements D" first="David Julian" last="Mcclements">David Julian Mcclements</name><affiliation wicri:level="1"><nlm:aff id="A2">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Food Science, University of Massachusetts, Amherst, MA</wicri:regionArea></affiliation></author><author><name sortKey="Xiao, Hang" sort="Xiao, Hang" uniqKey="Xiao H" first="Hang" last="Xiao">Hang Xiao</name><affiliation wicri:level="1"><nlm:aff id="A2">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Food Science, University of Massachusetts, Amherst, MA</wicri:regionArea></affiliation></author></analytic><series><title level="j">Journal of Agricultural and Food Chemistry</title><idno type="ISSN">0021-8561</idno><idno type="eISSN">1520-5118</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P2">Inflammation plays important roles in initiation and progress of many diseases including cancers in multiple organ sites. Herein, we investigated the anti-inflammatory effects of two dietary compounds, nobiletin (NBN) and sulforaphane (SFN) in combination. Non-cytotoxic concentrations of NBN, SFN, and their combinations were studied in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. The results showed that combined NBN and SFN treatments produced much stronger inhibitory effects on the production of nitric oxide (NO) than NBN or SFN alone at higher concentrations. These enhanced inhibitory effects were synergistic based on the isobologram analysis. Western blot analysis showed that combined NBN and SFN treatments synergistically decreased iNOS and COX-2 protein expression levels and induced heme oxygenase-1 (HO-1) protein expression. Real-time PCR analysis indicated that low doses of NBN and SFN in combination significantly suppressed LPS-induced upregulation of IL-1 mRNA levels, and synergistically increased HO-1 mRNA levels. Overall our results demonstrated that NBN and SFN in combination produced synergistic effects in inhibiting LPS-induced inflammation in RAW 264.7 cells.</p></div></front></TEI><pmc article-type="research-article" xml:lang="en"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">0374755</journal-id><journal-id journal-id-type="pubmed-jr-id">4421</journal-id><journal-id journal-id-type="nlm-ta">J Agric Food Chem</journal-id><journal-title-group><journal-title>Journal of Agricultural and Food Chemistry</journal-title></journal-title-group><issn pub-type="ppub">0021-8561</issn><issn pub-type="epub">1520-5118</issn></journal-meta><article-meta><article-id pub-id-type="pmid">22335189</article-id><article-id pub-id-type="pmc">3296826</article-id><article-id pub-id-type="doi">10.1021/jf300129t</article-id><article-id pub-id-type="manuscript">NIHMS359934</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Synergistic anti-inflammatory effects of Nobiletin and Sulforaphane in lipopolysaccharide-stimulated RAW 264.7 cells</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Guo</surname><given-names>Shanshan</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Qiu</surname><given-names>Peiju</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Xu</surname><given-names>Guang</given-names></name><xref ref-type="aff" rid="A3">3</xref></contrib><contrib contrib-type="author"><name><surname>Wu</surname><given-names>Xian</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Dong</surname><given-names>Ping</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Yang</surname><given-names>Guanpin</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Zheng</surname><given-names>Jinkai</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>McClements</surname><given-names>David Julian</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Xiao</surname><given-names>Hang</given-names></name><xref ref-type="aff" rid="A2">2</xref><xref ref-type="corresp" rid="CR1">*</xref></contrib></contrib-group><aff id="A1"><label>1</label>College of Marine Life Science, Ocean University of China, Qingdao, Shandong, P. R. China</aff><aff id="A2"><label>2</label>Department of Food Science, University of Massachusetts, Amherst, MA, USA</aff><aff id="A3"><label>3</label>Department of Plant, Soil & Insect Sciences, University of Massachusetts, Amherst, MA, USA</aff><author-notes><fn id="FN1"><p id="P1">The authors have declared no conflict of interest.</p></fn><corresp id="CR1"><label>*</label><bold>Corresponding Author:</bold> Hang Xiao Department of Food Science University of Massachusetts 100 Holdsworth Way Amherst, MA 01003, USA Tel: (413) 545-2281; Fax: (413) 545-1262 <email>hangxiao@foodsci.umass.edu</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>29</day><month>2</month><year>2012</year></pub-date><pub-date pub-type="epub"><day>27</day><month>2</month><year>2012</year></pub-date><pub-date pub-type="ppub"><day>7</day><month>3</month><year>2012</year></pub-date><pub-date pub-type="pmc-release"><day>7</day><month>3</month><year>2013</year></pub-date><volume>60</volume><issue>9</issue><fpage>2157</fpage><lpage>2164</lpage><abstract><p id="P2">Inflammation plays important roles in initiation and progress of many diseases including cancers in multiple organ sites. Herein, we investigated the anti-inflammatory effects of two dietary compounds, nobiletin (NBN) and sulforaphane (SFN) in combination. Non-cytotoxic concentrations of NBN, SFN, and their combinations were studied in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. The results showed that combined NBN and SFN treatments produced much stronger inhibitory effects on the production of nitric oxide (NO) than NBN or SFN alone at higher concentrations. These enhanced inhibitory effects were synergistic based on the isobologram analysis. Western blot analysis showed that combined NBN and SFN treatments synergistically decreased iNOS and COX-2 protein expression levels and induced heme oxygenase-1 (HO-1) protein expression. Real-time PCR analysis indicated that low doses of NBN and SFN in combination significantly suppressed LPS-induced upregulation of IL-1 mRNA levels, and synergistically increased HO-1 mRNA levels. Overall our results demonstrated that NBN and SFN in combination produced synergistic effects in inhibiting LPS-induced inflammation in RAW 264.7 cells.</p></abstract><kwd-group><kwd>Nobiletin</kwd><kwd>sulforaphane</kwd><kwd>inflammation</kwd><kwd>nitric oxide</kwd><kwd>iNOS</kwd><kwd>COX-2</kwd></kwd-group><funding-group><award-group><funding-source country="United States">National Cancer Institute : NCI</funding-source><award-id>R21 CA139174-02 || CA</award-id></award-group><award-group><funding-source country="United States">National Cancer Institute : NCI</funding-source><award-id>R21 CA139174-01A2 || CA</award-id></award-group></funding-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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