Data on antioxidant activity in grapevine (Vitis vinifera L.) following cryopreservation by vitrification
Identifieur interne : 000A27 ( Pmc/Curation ); précédent : 000A26; suivant : 000A28Data on antioxidant activity in grapevine (Vitis vinifera L.) following cryopreservation by vitrification
Auteurs : María Fernanda Lazo-Javalera [Mexique] ; Martín Ernesto Tiznado-Hernández [Mexique] ; Irasema Vargas-Arispuro [Mexique] ; Elisa Valenzuela-Soto [Mexique] ; María Del Carmen Rocha-Granados [Mexique] ; Marcos Edel Martínez-Montero [Cuba] ; Marisela Rivera-Domínguez [Mexique]Source :
- Data in Brief [ 2352-3409 ] ; 2015.
Abstract
Cryopreservation is used for the long-term conservation of plant genetic resources. This technique very often induces lethal injury or tissue damage. In this study, we measured indicators of viability and cell damage following cryopreservation and vitrification-cryopreservation in
Url:
DOI: 10.1016/j.dib.2015.10.012
PubMed: 26958607
PubMed Central: 4773358
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Data on antioxidant activity in grapevine (<italic>Vitis vinifera</italic> L.) following cryopreservation by vitrification</title><author><name sortKey="Lazo Javalera, Maria Fernanda" sort="Lazo Javalera, Maria Fernanda" uniqKey="Lazo Javalera M" first="María Fernanda" last="Lazo-Javalera">María Fernanda Lazo-Javalera</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000</wicri:regionArea></affiliation></author><author><name sortKey="Tiznado Hernandez, Martin Ernesto" sort="Tiznado Hernandez, Martin Ernesto" uniqKey="Tiznado Hernandez M" first="Martín Ernesto" last="Tiznado-Hernández">Martín Ernesto Tiznado-Hernández</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Research Center of Food and Development, A.C. Vegetal Origin Food Technology Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Research Center of Food and Development, A.C. Vegetal Origin Food Technology Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000</wicri:regionArea></affiliation></author><author><name sortKey="Vargas Arispuro, Irasema" sort="Vargas Arispuro, Irasema" uniqKey="Vargas Arispuro I" first="Irasema" last="Vargas-Arispuro">Irasema Vargas-Arispuro</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000</wicri:regionArea></affiliation></author><author><name sortKey="Valenzuela Soto, Elisa" sort="Valenzuela Soto, Elisa" uniqKey="Valenzuela Soto E" first="Elisa" last="Valenzuela-Soto">Elisa Valenzuela-Soto</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000</wicri:regionArea></affiliation></author><author><name sortKey="Rocha Granados, Maria Del Carmen" sort="Rocha Granados, Maria Del Carmen" uniqKey="Rocha Granados M" first="María Del Carmen" last="Rocha-Granados">María Del Carmen Rocha-Granados</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Plant Molecular Biology Laboratory. Michoacana University de San Nicolás de Hidalgo, Paseo Gral. Lázaro Cárdenas y Berlín S/N, Col. Viveros, C.P. 60170 Uruapan, Michoacán, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Plant Molecular Biology Laboratory. Michoacana University de San Nicolás de Hidalgo, Paseo Gral. Lázaro Cárdenas y Berlín S/N, Col. Viveros, C.P. 60170 Uruapan, Michoacán</wicri:regionArea></affiliation></author><author><name sortKey="Martinez Montero, Marcos Edel" sort="Martinez Montero, Marcos Edel" uniqKey="Martinez Montero M" first="Marcos Edel" last="Martínez-Montero">Marcos Edel Martínez-Montero</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Bioplants Center, Plant Breeding Laboratory University of Ciego de Avila, Car. a Moron km 9, CP 69450 Ciego de Avila, Cuba</nlm:aff><country xml:lang="fr">Cuba</country><wicri:regionArea>Bioplants Center, Plant Breeding Laboratory University of Ciego de Avila, Car. a Moron km 9, CP 69450 Ciego de Avila</wicri:regionArea></affiliation></author><author><name sortKey="Rivera Dominguez, Marisela" sort="Rivera Dominguez, Marisela" uniqKey="Rivera Dominguez M" first="Marisela" last="Rivera-Domínguez">Marisela Rivera-Domínguez</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26958607</idno><idno type="pmc">4773358</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773358</idno><idno type="RBID">PMC:4773358</idno><idno type="doi">10.1016/j.dib.2015.10.012</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000A28</idno><idno type="wicri:Area/Pmc/Curation">000A27</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Data on antioxidant activity in grapevine (<italic>Vitis vinifera</italic> L.) following cryopreservation by vitrification</title><author><name sortKey="Lazo Javalera, Maria Fernanda" sort="Lazo Javalera, Maria Fernanda" uniqKey="Lazo Javalera M" first="María Fernanda" last="Lazo-Javalera">María Fernanda Lazo-Javalera</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000</wicri:regionArea></affiliation></author><author><name sortKey="Tiznado Hernandez, Martin Ernesto" sort="Tiznado Hernandez, Martin Ernesto" uniqKey="Tiznado Hernandez M" first="Martín Ernesto" last="Tiznado-Hernández">Martín Ernesto Tiznado-Hernández</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Research Center of Food and Development, A.C. Vegetal Origin Food Technology Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Research Center of Food and Development, A.C. Vegetal Origin Food Technology Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000</wicri:regionArea></affiliation></author><author><name sortKey="Vargas Arispuro, Irasema" sort="Vargas Arispuro, Irasema" uniqKey="Vargas Arispuro I" first="Irasema" last="Vargas-Arispuro">Irasema Vargas-Arispuro</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000</wicri:regionArea></affiliation></author><author><name sortKey="Valenzuela Soto, Elisa" sort="Valenzuela Soto, Elisa" uniqKey="Valenzuela Soto E" first="Elisa" last="Valenzuela-Soto">Elisa Valenzuela-Soto</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000</wicri:regionArea></affiliation></author><author><name sortKey="Rocha Granados, Maria Del Carmen" sort="Rocha Granados, Maria Del Carmen" uniqKey="Rocha Granados M" first="María Del Carmen" last="Rocha-Granados">María Del Carmen Rocha-Granados</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Plant Molecular Biology Laboratory. Michoacana University de San Nicolás de Hidalgo, Paseo Gral. Lázaro Cárdenas y Berlín S/N, Col. Viveros, C.P. 60170 Uruapan, Michoacán, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Plant Molecular Biology Laboratory. Michoacana University de San Nicolás de Hidalgo, Paseo Gral. Lázaro Cárdenas y Berlín S/N, Col. Viveros, C.P. 60170 Uruapan, Michoacán</wicri:regionArea></affiliation></author><author><name sortKey="Martinez Montero, Marcos Edel" sort="Martinez Montero, Marcos Edel" uniqKey="Martinez Montero M" first="Marcos Edel" last="Martínez-Montero">Marcos Edel Martínez-Montero</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Bioplants Center, Plant Breeding Laboratory University of Ciego de Avila, Car. a Moron km 9, CP 69450 Ciego de Avila, Cuba</nlm:aff><country xml:lang="fr">Cuba</country><wicri:regionArea>Bioplants Center, Plant Breeding Laboratory University of Ciego de Avila, Car. a Moron km 9, CP 69450 Ciego de Avila</wicri:regionArea></affiliation></author><author><name sortKey="Rivera Dominguez, Marisela" sort="Rivera Dominguez, Marisela" uniqKey="Rivera Dominguez M" first="Marisela" last="Rivera-Domínguez">Marisela Rivera-Domínguez</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000</wicri:regionArea></affiliation></author></analytic><series><title level="j">Data in Brief</title><idno type="eISSN">2352-3409</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Cryopreservation is used for the long-term conservation of plant genetic resources. This technique very often induces lethal injury or tissue damage. In this study, we measured indicators of viability and cell damage following cryopreservation and vitrification-cryopreservation in <italic>Vitis vinifera</italic> L. axillary buds cv. “Flame seedless” stored in liquid nitrogen (LN) for: three seconds, one hour, one day, one week and one month; after LN thawed at 38 °C for three minutes. The enzymatic activity of catalase (CAT) and superoxide dismutase (SOD), as well as the amount of malondialdehyde (MDA), total protein and viability were assayed.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Matsumoto, T" uniqKey="Matsumoto T">T. Matsumoto</name></author><author><name sortKey="Sakai, A" uniqKey="Sakai A">A. Sakai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sakai, A" uniqKey="Sakai A">A. Sakai</name></author><author><name sortKey="Kobayashi, S" uniqKey="Kobayashi S">S. Kobayashi</name></author><author><name sortKey="Oiyama, I" uniqKey="Oiyama I">I. Oiyama</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Steponkus, P L" uniqKey="Steponkus P">P.L. Steponkus</name></author><author><name sortKey="Lanphear, F O" uniqKey="Lanphear F">F.O. Lanphear</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shuhaida, N" uniqKey="Shuhaida N">N. Shuhaida</name></author><author><name sortKey="Keng, C L" uniqKey="Keng C">C.L. Keng</name></author><author><name sortKey="Rathinam, X" uniqKey="Rathinam X">X. Rathinam</name></author><author><name sortKey="Sinnia, S" uniqKey="Sinnia S">S. Sinnia</name></author><author><name sortKey="Subramaniam, S" uniqKey="Subramaniam S">S. Subramaniam</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alotto, D" uniqKey="Alotto D">D. Alotto</name></author><author><name sortKey="Ariotti, S" uniqKey="Ariotti S">S. Ariotti</name></author><author><name sortKey="Graziano, S" uniqKey="Graziano S">S. Graziano</name></author><author><name sortKey="Verrua, R" uniqKey="Verrua R">R. Verrua</name></author><author><name sortKey="Stella, M" uniqKey="Stella M">M. Stella</name></author><author><name sortKey="Magliacani, G" uniqKey="Magliacani G">G. Magliacani</name></author><author><name sortKey="Castagnoli, C" uniqKey="Castagnoli C">C. Castagnoli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ramirez Serrano, R" uniqKey="Ramirez Serrano R">R. Ramírez-Serrano</name></author><author><name sortKey="Larrinaga Mayoral, J" uniqKey="Larrinaga Mayoral J">J. Larrinaga-Mayoral</name></author><author><name sortKey="Murillo Amador, B" uniqKey="Murillo Amador B">B. Murillo-Amador</name></author><author><name sortKey="Hernandez Saavedra, N" uniqKey="Hernandez Saavedra N">N. Hernández-Saavedra</name></author><author><name sortKey="Fujiyama, H" uniqKey="Fujiyama H">H. 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Bradford</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="other"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Data Brief</journal-id><journal-id journal-id-type="iso-abbrev">Data Brief</journal-id><journal-title-group><journal-title>Data in Brief</journal-title></journal-title-group><issn pub-type="epub">2352-3409</issn><publisher><publisher-name>Elsevier</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26958607</article-id><article-id pub-id-type="pmc">4773358</article-id><article-id pub-id-type="publisher-id">S2352-3409(15)00260-7</article-id><article-id pub-id-type="doi">10.1016/j.dib.2015.10.012</article-id><article-categories><subj-group subj-group-type="heading"><subject>Data Article</subject></subj-group></article-categories><title-group><article-title>Data on antioxidant activity in grapevine (<italic>Vitis vinifera</italic> L.) following cryopreservation by vitrification</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Lazo-Javalera</surname><given-names>María Fernanda</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Tiznado-Hernández</surname><given-names>Martín Ernesto</given-names></name><xref rid="aff0010" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Vargas-Arispuro</surname><given-names>Irasema</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Valenzuela-Soto</surname><given-names>Elisa</given-names></name><xref rid="aff0005" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Rocha-Granados</surname><given-names>María del Carmen</given-names></name><xref rid="aff0015" ref-type="aff">c</xref></contrib><contrib contrib-type="author"><name><surname>Martínez-Montero</surname><given-names>Marcos Edel</given-names></name><xref rid="aff0020" ref-type="aff">d</xref></contrib><contrib contrib-type="author"><name><surname>Rivera-Domínguez</surname><given-names>Marisela</given-names></name><email>marisela@ciad.mx</email><xref rid="aff0005" ref-type="aff">a</xref><xref rid="cor1" ref-type="corresp">⁎</xref></contrib></contrib-group><aff id="aff0005"><label>a</label>Research Center of Food and Development, A.C. Biotechnology and Plant Molecular Biology Laboratory. Food Science Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico</aff><aff id="aff0010"><label>b</label>Research Center of Food and Development, A.C. Vegetal Origin Food Technology Coordination, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83000, Mexico</aff><aff id="aff0015"><label>c</label>Plant Molecular Biology Laboratory. Michoacana University de San Nicolás de Hidalgo, Paseo Gral. Lázaro Cárdenas y Berlín S/N, Col. Viveros, C.P. 60170 Uruapan, Michoacán, Mexico</aff><aff id="aff0020"><label>d</label>Bioplants Center, Plant Breeding Laboratory University of Ciego de Avila, Car. a Moron km 9, CP 69450 Ciego de Avila, Cuba</aff><author-notes><corresp id="cor1"><label>⁎</label>Corresponding author. <email>marisela@ciad.mx</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>21</day><month>10</month><year>2015</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="collection"><month>12</month><year>2015</year></pub-date><pub-date pub-type="epub"><day>21</day><month>10</month><year>2015</year></pub-date><volume>5</volume><fpage>549</fpage><lpage>555</lpage><history><date date-type="received"><day>11</day><month>9</month><year>2015</year></date><date date-type="rev-recd"><day>8</day><month>10</month><year>2015</year></date><date date-type="accepted"><day>13</day><month>10</month><year>2015</year></date></history><permissions><copyright-statement>© 2015 The Authors</copyright-statement><copyright-year>2015</copyright-year><license license-type="CC BY" xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).</license-p></license></permissions><abstract><p>Cryopreservation is used for the long-term conservation of plant genetic resources. This technique very often induces lethal injury or tissue damage. In this study, we measured indicators of viability and cell damage following cryopreservation and vitrification-cryopreservation in <italic>Vitis vinifera</italic> L. axillary buds cv. “Flame seedless” stored in liquid nitrogen (LN) for: three seconds, one hour, one day, one week and one month; after LN thawed at 38 °C for three minutes. The enzymatic activity of catalase (CAT) and superoxide dismutase (SOD), as well as the amount of malondialdehyde (MDA), total protein and viability were assayed.</p></abstract></article-meta></front><floats-group><fig id="f0005"><label>Fig. 1</label><caption><p>Viability (%) in ‘Flame seedless’ grapevine buds. Cryopreserved (a) and vitrified-cryopreserved (b). C: buds without treatment, I: buds stored in LN for 3 s, H: buds stored in LN for one hour, D: buds stored in LN for one day, W: buds stored in LN for one week, M: buds stored in LN for one month. Black-colored bars indicate buds without thawing; grey-colored bars indicate buds thawed at 38 °C for 3 min. Different letters indicate differences between treatments according to the Tukey–Kramer test.</p></caption><alt-text id="at0005">Fig. 1.</alt-text><graphic xlink:href="gr1"></graphic></fig><fig id="f0010"><label>Fig. 2</label><caption><p>Catalase activity (a,b) and superoxide dismutase activity (c,d) (U/mg protein) in ‘Flame seedless’ grapevine buds. Cryopreserved (a,c) and vitrified-cryopreserved (b,d). C: buds without treatment, P: buds immersed in cryoprotective solution, I: buds stored in LN for 3 s, H: buds stored in LN for one hour, D: buds stored in LN for one day, W: buds stored in LN for one week, M: buds stored in LN for one month. Black-colored bars indicate buds without thawing; grey-colored bars indicate buds thawed at 38 °C for 3 min. Different letters indicate differences between treatments according to the Tukey–Kramer test.</p></caption><alt-text id="at0010">Fig. 2.</alt-text><graphic xlink:href="gr2"></graphic></fig><fig id="f0015"><label>Fig. 3</label><caption><p>Malondialdehyde (MDA) content (µmol g<sup>−1</sup> of fresh weight) in grapevine buds cryopreserved and vitrified-cryopreserved for different times of storage in LN. (a) Buds without thawing and (b) buds thawed at 38 °C for 3 min. C: buds without treatment; P: buds immersed in cryoprotective solution, I: buds stored in LN for 3 s, H: buds stored in LN for one hour, D: buds stored in LN for one day, W: buds stored in LN for one week, M: buds stored in LN for one month. Black-colored bars indicate buds treated or not (white bars) with PVS2 solution and stored in LN. Different letters indicate differences between treatments according to the Tukey–Kramer test.</p></caption><alt-text id="at0015">Fig. 3.</alt-text><graphic xlink:href="gr3"></graphic></fig><table-wrap id="t0005" position="float"><label>Table 1</label><caption><p>Effect of thawing on total protein content (µg g<sup>−1</sup>) of fresh weight in ‘Flame seedless’ grapevine buds treated for different times with vitrification or vitrification-cryopreservation.</p></caption><alt-text id="at0020">Table 1</alt-text><table frame="hsides" rules="groups"><thead><tr><th></th><th colspan="2">Vitrification-cryopreservation<hr></hr></th><th colspan="2">Cryopreservation<hr></hr></th></tr><tr><th>Time in LN</th><th>Without thawing Mean±SD (µg g<sup>−1</sup>FW)</th><th>Thawing (38 °C/3 min) Mean±SD (µg g<sup>−1</sup>FW)</th><th>Without thawing Mean±SD (µg g<sup>−1</sup>FW)</th><th>Thawing (38 °C/3 min) Mean±SD (µg g<sup>−1</sup>FW)</th></tr></thead><tbody><tr><td>Control</td><td>1.9±1.7</td><td>1.9±1.7</td><td>1.9±1.7</td><td>1.9±1.7</td></tr><tr><td>PVS2</td><td>20.9±6.7</td><td>–</td><td>–</td><td>–</td></tr><tr><td>Initial</td><td>–</td><td>33.9±3.9<xref rid="tbl1fnStar" ref-type="table-fn">⁎</xref></td><td>–</td><td>4.1±2.2</td></tr><tr><td>1 h</td><td>27.9±1.6</td><td>10.8±4.1</td><td>5.3±3.2</td><td>10.4±1.5</td></tr><tr><td>1 day</td><td>16.4±5.1</td><td>19.9±4.5</td><td>8.8±2.4</td><td>5.2±4.4</td></tr><tr><td>1 week</td><td>28.1±11.2</td><td>21.8±5.5</td><td>1.6±0.6</td><td>1.5±1.5</td></tr><tr><td>1 month</td><td>48.5±10.4<xref rid="tbl1fnStar" ref-type="table-fn">⁎</xref></td><td>37.0±5.9<xref rid="tbl1fnStar" ref-type="table-fn">⁎</xref></td><td>24.4±3.7</td><td>18.3±5.4</td></tr></tbody></table><table-wrap-foot><fn><p>PVS2 refers to treatment by immersion into cryoprotective solution only. Values represent means ± standard deviation.</p></fn></table-wrap-foot><table-wrap-foot><fn id="tbl1fnStar"><label>*</label><p id="ntp0005">Indicates differences (<italic>P</italic><0.05) among treatments according to the Tukey–Kramer test.</p></fn></table-wrap-foot></table-wrap></floats-group></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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