Characterization of a Citrus R2R3-MYB Transcription Factor that Regulates the Flavonol and Hydroxycinnamic Acid Biosynthesis
Identifieur interne : 000463 ( Pmc/Curation ); précédent : 000462; suivant : 000464Characterization of a Citrus R2R3-MYB Transcription Factor that Regulates the Flavonol and Hydroxycinnamic Acid Biosynthesis
Auteurs : Chaoyang Liu [République populaire de Chine] ; Jianmei Long [République populaire de Chine] ; Kaijie Zhu [République populaire de Chine] ; Linlin Liu [République populaire de Chine] ; Wei Yang [République populaire de Chine] ; Hongyan Zhang [République populaire de Chine] ; Li Li [États-Unis] ; Qiang Xu [République populaire de Chine] ; Xiuxin Deng [République populaire de Chine]Source :
- Scientific Reports [ 2045-2322 ] ; 2016.
Abstract
Flavonols and hydroxycinnamic acids are important phenylpropanoid metabolites in plants. In this study, we isolated and characterized a citrus R2R3-MYB transcription factor
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DOI: 10.1038/srep25352
PubMed: 27162196
PubMed Central: 4861916
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of a Citrus R2R3-MYB Transcription Factor that Regulates the Flavonol and Hydroxycinnamic Acid Biosynthesis</title><author><name sortKey="Liu, Chaoyang" sort="Liu, Chaoyang" uniqKey="Liu C" first="Chaoyang" last="Liu">Chaoyang Liu</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Long, Jianmei" sort="Long, Jianmei" uniqKey="Long J" first="Jianmei" last="Long">Jianmei Long</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhu, Kaijie" sort="Zhu, Kaijie" uniqKey="Zhu K" first="Kaijie" last="Zhu">Kaijie Zhu</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Linlin" sort="Liu, Linlin" uniqKey="Liu L" first="Linlin" last="Liu">Linlin Liu</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Yang, Wei" sort="Yang, Wei" uniqKey="Yang W" first="Wei" last="Yang">Wei Yang</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Hongyan" sort="Zhang, Hongyan" uniqKey="Zhang H" first="Hongyan" last="Zhang">Hongyan Zhang</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Li, Li" sort="Li, Li" uniqKey="Li L" first="Li" last="Li">Li Li</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Robert W. Holley Center for Agriculture and Health, USDA-ARS, Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University</institution>, Ithaca, NY 14853,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Xu, Qiang" sort="Xu, Qiang" uniqKey="Xu Q" first="Qiang" last="Xu">Qiang Xu</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Deng, Xiuxin" sort="Deng, Xiuxin" uniqKey="Deng X" first="Xiuxin" last="Deng">Xiuxin Deng</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27162196</idno><idno type="pmc">4861916</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861916</idno><idno type="RBID">PMC:4861916</idno><idno type="doi">10.1038/srep25352</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000464</idno><idno type="wicri:Area/Pmc/Curation">000463</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Characterization of a Citrus R2R3-MYB Transcription Factor that Regulates the Flavonol and Hydroxycinnamic Acid Biosynthesis</title><author><name sortKey="Liu, Chaoyang" sort="Liu, Chaoyang" uniqKey="Liu C" first="Chaoyang" last="Liu">Chaoyang Liu</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Long, Jianmei" sort="Long, Jianmei" uniqKey="Long J" first="Jianmei" last="Long">Jianmei Long</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhu, Kaijie" sort="Zhu, Kaijie" uniqKey="Zhu K" first="Kaijie" last="Zhu">Kaijie Zhu</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Linlin" sort="Liu, Linlin" uniqKey="Liu L" first="Linlin" last="Liu">Linlin Liu</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Yang, Wei" sort="Yang, Wei" uniqKey="Yang W" first="Wei" last="Yang">Wei Yang</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Hongyan" sort="Zhang, Hongyan" uniqKey="Zhang H" first="Hongyan" last="Zhang">Hongyan Zhang</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Li, Li" sort="Li, Li" uniqKey="Li L" first="Li" last="Li">Li Li</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Robert W. Holley Center for Agriculture and Health, USDA-ARS, Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University</institution>, Ithaca, NY 14853,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Xu, Qiang" sort="Xu, Qiang" uniqKey="Xu Q" first="Qiang" last="Xu">Qiang Xu</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Deng, Xiuxin" sort="Deng, Xiuxin" uniqKey="Deng X" first="Xiuxin" last="Deng">Xiuxin Deng</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Flavonols and hydroxycinnamic acids are important phenylpropanoid metabolites in plants. In this study, we isolated and characterized a citrus R2R3-MYB transcription factor <italic>CsMYBF1</italic>, encoding a protein belonging to the flavonol-specific MYB subgroup. Ectopic expression of <italic>CsMYBF1</italic> in tomato led to an up-regulation of a series of genes involved in primary metabolism and the phenylpropanoid pathway, and induced a strong accumulation of hydroxycinnamic acid compounds but not the flavonols. The RNAi suppression of <italic>CsMYBF1</italic> in citrus callus caused a down-regulation of many phenylpropanoid pathway genes and reduced the contents of hydroxycinnamic acids and flavonols. Transactivation assays indicated that CsMYBF1 activated several promoters of phenylpropanoid pathway genes in tomato and citrus. Interestingly, CsMYBF1 could activate the <italic>CHS</italic> gene promoter in citrus, but not in tomato. Further examinations revealed that the MYBPLANT cis-elements were essential for CsMYBF1 in activating phenylpropanoid pathway genes. In summary, our data indicated that CsMYBF1 possessed the function in controlling the flavonol and hydroxycinnamic acid biosynthesis, and the regulatory differences in the target metabolite accumulation between two species may be due to the differential activation of <italic>CHS</italic> promoters by CsMYBF1. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Sci Rep</journal-id><journal-id journal-id-type="iso-abbrev">Sci Rep</journal-id><journal-title-group><journal-title>Scientific Reports</journal-title></journal-title-group><issn pub-type="epub">2045-2322</issn><publisher><publisher-name>Nature Publishing Group</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27162196</article-id><article-id pub-id-type="pmc">4861916</article-id><article-id pub-id-type="pii">srep25352</article-id><article-id pub-id-type="doi">10.1038/srep25352</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Characterization of a Citrus R2R3-MYB Transcription Factor that Regulates the Flavonol and Hydroxycinnamic Acid Biosynthesis</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Chaoyang</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Long</surname><given-names>Jianmei</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Zhu</surname><given-names>Kaijie</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Linlin</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Yang</surname><given-names>Wei</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Hongyan</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Li</given-names></name><xref ref-type="aff" rid="a2">2</xref></contrib><contrib contrib-type="author"><name><surname>Xu</surname><given-names>Qiang</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Deng</surname><given-names>Xiuxin</given-names></name><xref ref-type="corresp" rid="c1">a</xref><xref ref-type="aff" rid="a1">1</xref></contrib><aff id="a1"><label>1</label><institution>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University</institution>, Wuhan 430070,<country>P. R. China</country></aff><aff id="a2"><label>2</label><institution>Robert W. Holley Center for Agriculture and Health, USDA-ARS, Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University</institution>, Ithaca, NY 14853,<country>USA</country></aff></contrib-group><author-notes><corresp id="c1"><label>a</label><email>xxdeng@mail.hzau.edu.cn</email></corresp></author-notes><pub-date pub-type="epub"><day>10</day><month>05</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>6</volume><elocation-id>25352</elocation-id><history><date date-type="received"><day>28</day><month>01</month><year>2016</year></date><date date-type="accepted"><day>12</day><month>04</month><year>2016</year></date></history><permissions><copyright-statement>Copyright © 2016, Macmillan Publishers Limited</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>Macmillan Publishers Limited</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/"><pmc-comment>author-paid</pmc-comment>
<license-p>This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link></license-p></license></permissions><abstract><p>Flavonols and hydroxycinnamic acids are important phenylpropanoid metabolites in plants. In this study, we isolated and characterized a citrus R2R3-MYB transcription factor <italic>CsMYBF1</italic>, encoding a protein belonging to the flavonol-specific MYB subgroup. Ectopic expression of <italic>CsMYBF1</italic> in tomato led to an up-regulation of a series of genes involved in primary metabolism and the phenylpropanoid pathway, and induced a strong accumulation of hydroxycinnamic acid compounds but not the flavonols. The RNAi suppression of <italic>CsMYBF1</italic> in citrus callus caused a down-regulation of many phenylpropanoid pathway genes and reduced the contents of hydroxycinnamic acids and flavonols. Transactivation assays indicated that CsMYBF1 activated several promoters of phenylpropanoid pathway genes in tomato and citrus. Interestingly, CsMYBF1 could activate the <italic>CHS</italic> gene promoter in citrus, but not in tomato. Further examinations revealed that the MYBPLANT cis-elements were essential for CsMYBF1 in activating phenylpropanoid pathway genes. In summary, our data indicated that CsMYBF1 possessed the function in controlling the flavonol and hydroxycinnamic acid biosynthesis, and the regulatory differences in the target metabolite accumulation between two species may be due to the differential activation of <italic>CHS</italic> promoters by CsMYBF1. Therefore, <italic>CsMYBF1</italic> constitutes an important gene source for the engineering of specific phenylpropanoid components.</p></abstract></article-meta></front><floats-group><fig id="f1"><label>Figure 1</label><caption><title>Schematic representation of shikimate and phenylpropanoid biosynthesis pathways in plants.</title><p>Enzyme names are abbreviated as follows: DHAPS, DAHP synthase; DHQS, dehydroquinate synthase; DHD-SDH, 3-dehydroquinate dehydratase/shikimate 5-dehydrogenase; SK, shikimate kinase; EPSPS, EPSP synthase; CS, chorismate synthase; PAL, phenylalanine ammonia lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-hydroxy-cinnamoyl CoA ligase; C3H, ρ-Coumarate 3-hydrolase; COMT, caffeic acid 3-O-methyltransferase; CCR, cinnamoyl CoA reductase; CAD, cinnamyl alcohol dehydrogenase; CHS, chalcone synthase; CHI, chalcone isomerase; F3H, flavanone-3-hydroxylase; F3′H, flavonoid-3′-hydroxylase; F3′5′H, flavonoid-3′5′-hydroxylase; FLS, flavonol synthase; FNS, flavone synthase; HCT, cinnamoyl CoA shikimate/quinate transferase; HQT, hydroxycinnamoyl CoA quinate transferase.</p></caption><graphic xlink:href="srep25352-f1"></graphic></fig><fig id="f2"><label>Figure 2</label><caption><title>Sequence comparison, expression pattern and subcellular localization of CsMYBF1.</title><p>(<bold>a</bold>) Phylogenetic analysis of selected plant MYB proteins. The scale bar represents 0.05 substitutions per site. GenBank accession numbers of the proteins are listed in <xref ref-type="supplementary-material" rid="S1">Supplementary Table S1</xref>. (<bold>b</bold>) Expression pattern of <italic>CsMYBF1</italic> in different tissues of citrus. Data are means ± SE of three replicate PCRs. (<bold>c</bold>) The subcellular localization of CsMYBF1 in citrus protoplast. OsGhd7-CFP and CsMYBF1-GFP were co-transformed into citrus mesophyll protoplasts. OsGhd7-CFP was used as a nuclear marker. (i) OsGhd7-CFP , (ii) CsMYBF1-GFP, (iii) bright field, (iv) merged picture.</p></caption><graphic xlink:href="srep25352-f2"></graphic></fig><fig id="f3"><label>Figure 3</label><caption><title>Integrated metabolome and transcriptome analysis for the <italic>CsMYBF1</italic>-overexpressing tomato fruits.</title><p>Differences between metabolic profiles of WT/transgenic lines were adopted by PCA of LC-MS (<bold>a</bold>) and GC-MS data sets (<bold>b</bold>). (<bold>c</bold>) Hierarchical cluster analysis of secondary metabolites data in the WT and transgenic tomato fruits. (<bold>d</bold>) HPLC analysis of methanol extracts from WT and <italic>CsMYBF1</italic>-overexpressing tomato fruits. S1, 3-Caffeoylquinic acid; S2, 4-Caffeoylquinic acid; S3, Quercetin glucosyl-glucoside rhamnoside; S4, Kaempferol glucosyl-glucoside rhamnoside; S5, Rutin; S6, Phloretin 3′,5′-di-C-glucoside; S7, Kaempferol rutinoside; S8, Dicaffeoylquinic acid; S9, Dicaffeoylquinic acid; S10, Naringenin chalcone-glucoside; S11, Tricaffeoylquinic acid; S12, Naringenin chalcone (<bold>e</bold>) Hierarchical cluster analysis of primary metabolites data in the WT and transgenic tomato fruits. Data were processed with Z-score transformation and hierarchically clustered using Spearman distance. (<bold>f</bold>) Analysis of transcript levels of endogenous phenylpropanoid related genes in transgenic tomato fruits. Data are means ± SE of three replicate PCRs. Asterisks (P < 0.05, Student’s t-test) indicate significant differences compared with WT.</p></caption><graphic xlink:href="srep25352-f3"></graphic></fig><fig id="f4"><label>Figure 4</label><caption><title>Effects of RNAi suppression of <italic>CsMYBF1</italic> in citrus callus on the endogenous gene expression and metabolite accumulation.</title><p>Expression analyses of <italic>CsMYBF1</italic> expression in WT and transgenic lines by quantitative RT-PCR (<bold>a</bold>) and semi-quantitative RT-PCR (<bold>b</bold>). (<bold>c</bold>) Contents of five representative phenylpropanoid compounds in WT and transgenic callus lines determined by LC-MS. Data are means of three replicates and error bars indicating SD. DW, dry weight. Asterisks indicate significant differences as determined by t-test analysis (<italic>P</italic> < 0.05). (<bold>d</bold>) Differences between metabolic profiles of WT and RNAi callus lines detected by PCA of LC-MS data sets. (<bold>e</bold>) Visualized flavonol accumulation in transgenic calli. Representative images for the calli of WT (i), RNAi lines (ii) and negative control (iii) were shown. Bars = 1mm. (<bold>f</bold>) HPLC analysis of methanol extracts from WT and RNAi callus lines. C1, Kaempferol derivative; C2, Rutin; C3, Kaempferol-rutinside; C4, Quercetin derivative; C5, Kaempferol derivative; C6, Quercetin derivative (<bold>g</bold>) Analysis of transcript levels of endogenous phenylpropanoid biosynthesis genes in transgenic lines by quantitative RT-PCR. The WT expression data were normalized to 1. Data are means ± SD of two independent biological replicates. Asterisks (P < 0.05, Student’s t-test) indicate significant differences compared with WT.</p></caption><graphic xlink:href="srep25352-f4"></graphic></fig><fig id="f5"><label>Figure 5</label><caption><p>Effects of CsMYBF1 on promoter activities of phenylpropanoid pathway genes from tomato (<bold>a</bold>) and citrus (<bold>b</bold>) in transient expression assays. LUC, Firefly luciferase activity; REN, Renilla luciferase activity. The ratio of LUC/REN of the empty vector (EV) plus promoter was used as a calibrator (set as 1). Error bars indicate SE from six replicates. Asterisks indicate significant differences as determined by t-test analysis (<italic>P</italic> < 0.05).</p></caption><graphic xlink:href="srep25352-f5"></graphic></fig><fig id="f6"><label>Figure 6</label><caption><title>CsMYBF1 specifically activates the three selected promoters via the MYBPLANT cis-element.</title><p>Diagrams showing various DNA fragments of the three selected promoters inked to the firefly luciferase reporter: (<bold>a</bold>) ProCs4CL, (<bold>b</bold>) ProSlFLS and (<bold>c</bold>) ProCsCHS. The detailed promoter lengths and the position of the cis-elements were shown in Figure S4. (<bold>d</bold>) Diagrams showing four copies of wild type/mutant MYBPLANT cis-elements linked to the minimal 35S promoter and the firefly luciferase reporter. The corresponding relative ratio of LUC/REN was shown on the right. The ratio of LUC/REN of the empty vector (EV) plus promoter was used as a calibrator (set as 1). Error bars indicate SE from six replicates. Asterisks indicate significant differences as determined by t-test analysis (<italic>P</italic> < 0.05).</p></caption><graphic xlink:href="srep25352-f6"></graphic></fig><fig id="f7"><label>Figure 7</label><caption><title>Yeast one-hybrid assays.</title><p>(<bold>a</bold>) Schematic structures of the yeast one hybrid effector (pGADT7-CsMYBF1) and reporter vector. (<bold>b</bold>) Growth of yeast cells transformed with the effector plasmid and the reporter plasmid on SD-Trp-Leu-His supplemented with or without 50 mM 3-AT. P. Control, positive control (p53HIS2 plus pGAD-p53); N. Control, negative control (p53HIS2 plus pGAD-CsMYBF1)</p></caption><graphic xlink:href="srep25352-f7"></graphic></fig><fig id="f8"><label>Figure 8</label><caption><title>CsMYBF1 binds to the promoters containing MYBPLANT cis-elements.</title><p>(<bold>a</bold>) Sequences for oligonucleotides used in the EMSA. The red bold letters highlight MYBPLANT sequences, mutated positions are underlined. (<bold>b</bold>) EMSA of the CsMYBF1 binding to the promoters of <italic>Cs4CL</italic>, <italic>SlFLS</italic> and <italic>CsCHS</italic>. The “+” and “−” indicate the presence and absence of the corresponding probe or protein, respectively. Arrows indicate the positions of protein-DNA probe complex and free probes, respectively.</p></caption><graphic xlink:href="srep25352-f8"></graphic></fig><table-wrap position="float" id="t1"><label>Table 1</label><caption><title>Changes in secondary metabolites in <italic>CsMYBF1</italic>-overexpressing tomato fruits relative to the WT.</title></caption><table frame="hsides" rules="groups" border="1"><colgroup><col align="left"></col><col align="left"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col></colgroup><thead valign="bottom"><tr><th rowspan="2" align="left" valign="bottom" charoff="50">Trend</th><th rowspan="2" align="left" valign="bottom" charoff="50">Putative metabolite name</th><th colspan="5" align="center" valign="top" charoff="50">Content (mg/100 g DW)<hr></hr></th></tr><tr><th align="center" valign="top" charoff="50">WT</th><th align="center" valign="top" charoff="50">D7</th><th align="center" valign="top" charoff="50">C2</th><th align="center" valign="top" charoff="50">E2</th><th align="center" valign="top" charoff="50">D4</th></tr></thead><tbody valign="top"><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">Caffeic acid</td><td align="center" valign="top" charoff="50">1.67 ± 0.45</td><td align="center" valign="top" charoff="50"><bold>4.32 ± 1.27</bold></td><td align="center" valign="top" charoff="50"><bold>9.74 ± 4.51</bold></td><td align="center" valign="top" charoff="50"><bold>5.33 ± 0.68</bold></td><td align="center" valign="top" charoff="50"><bold>3.96 ± 0.12</bold></td></tr><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">Ferulic acid</td><td align="center" valign="top" charoff="50">1.1 ± 0.51</td><td align="center" valign="top" charoff="50"><bold>2.68 ± 0.65</bold></td><td align="center" valign="top" charoff="50"><bold>4.85 ± 2.19</bold></td><td align="center" valign="top" charoff="50"><bold>2.82 ± 0.24</bold></td><td align="center" valign="top" charoff="50"><bold>2.51 ± 0.65</bold></td></tr><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">Sinapic acid</td><td align="center" valign="top" charoff="50">0.75 ± 0.25</td><td align="center" valign="top" charoff="50"><bold>1.89 ± 0.78</bold></td><td align="center" valign="top" charoff="50"><bold>3.1 ± 1.05</bold></td><td align="center" valign="top" charoff="50"><bold>2.12 ± 0.59</bold></td><td align="center" valign="top" charoff="50"><bold>2.41 ± 0.41</bold></td></tr><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">3-Caffeoylquinic acid</td><td align="center" valign="top" charoff="50">24.56 ± 8.12</td><td align="center" valign="top" charoff="50"><bold>85.83 ± 29.13</bold></td><td align="center" valign="top" charoff="50"><bold>132.8 ± 41.71</bold></td><td align="center" valign="top" charoff="50"><bold>105.86 ± 6.52</bold></td><td align="center" valign="top" charoff="50"><bold>108.37 ± 13.55</bold></td></tr><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">4-Caffeoylquinic acid</td><td align="center" valign="top" charoff="50">2 ± 0.68</td><td align="center" valign="top" charoff="50"><bold>5.13 ± 2.29</bold></td><td align="center" valign="top" charoff="50"><bold>7.02 ± 0.54</bold></td><td align="center" valign="top" charoff="50"><bold>6.11 ± 0.31</bold></td><td align="center" valign="top" charoff="50"><bold>4.55 ± 0.6</bold></td></tr><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">5-Caffeoylquinic acid</td><td align="center" valign="top" charoff="50">1.25 ± 0.35</td><td align="center" valign="top" charoff="50"><bold>2.86 ± 0.4</bold></td><td align="center" valign="top" charoff="50"><bold>4.35 ± 0.39</bold></td><td align="center" valign="top" charoff="50"><bold>3.26 ± 0.39</bold></td><td align="center" valign="top" charoff="50"><bold>2.99 ± 0.69</bold></td></tr><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">Caffeic acid-hexose I</td><td align="center" valign="top" charoff="50">46.64 ± 21.16</td><td align="center" valign="top" charoff="50">81.98 ± 25.5</td><td align="center" valign="top" charoff="50"><bold>148.84 ± 6.93</bold></td><td align="center" valign="top" charoff="50"><bold>103.18 ± 19.37</bold></td><td align="center" valign="top" charoff="50">66.4 ± 18.08</td></tr><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">Caffeic acid-hexose II</td><td align="center" valign="top" charoff="50">8.88 ± 1.99</td><td align="center" valign="top" charoff="50">14.87 ± 5.44</td><td align="center" valign="top" charoff="50"><bold>20.87 ± 2.57</bold></td><td align="center" valign="top" charoff="50"><bold>17.73 ± 3.72</bold></td><td align="center" valign="top" charoff="50">10.13 ± 2.55</td></tr><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">Caffeic acid-hexose III</td><td align="center" valign="top" charoff="50">34.8 ± 12.79</td><td align="center" valign="top" charoff="50"><bold>90.07 ± 21.14</bold></td><td align="center" valign="top" charoff="50"><bold>171.25 ± 67.81</bold></td><td align="center" valign="top" charoff="50"><bold>100.2 ± 15.18</bold></td><td align="center" valign="top" charoff="50"><bold>62.24 ± 5.48</bold></td></tr><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">Ferulic acid-hexose</td><td align="center" valign="top" charoff="50">11.96 ± 2.68</td><td align="center" valign="top" charoff="50"><bold>23.76 ± 4.75</bold></td><td align="center" valign="top" charoff="50"><bold>39.49 ± 14.82</bold></td><td align="center" valign="top" charoff="50"><bold>23.3 ± 3.47</bold></td><td align="center" valign="top" charoff="50"><bold>21.18 ± 4.45</bold></td></tr><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">Sinapic acid hexose</td><td align="center" valign="top" charoff="50">1.51 ± 0.26</td><td align="center" valign="top" charoff="50"><bold>5.6 ± 1.93</bold></td><td align="center" valign="top" charoff="50"><bold>10.29 ± 3.56</bold></td><td align="center" valign="top" charoff="50"><bold>7.54 ± 2.35</bold></td><td align="center" valign="top" charoff="50"><bold>5.52 ± 2.11</bold></td></tr><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">Dicaffeoylquinic acid I</td><td align="center" valign="top" charoff="50">3.39 ± 1.68</td><td align="center" valign="top" charoff="50">13.98 ± 11.58</td><td align="center" valign="top" charoff="50"><bold>20.1 ± 2.23</bold></td><td align="center" valign="top" charoff="50"><bold>15.66 ± 1.77</bold></td><td align="center" valign="top" charoff="50">6.95 ± 3.15</td></tr><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">Dicaffeoylquinic acid II</td><td align="center" valign="top" charoff="50">2.8 ± 1.76</td><td align="center" valign="top" charoff="50">14.09 ± 10.57</td><td align="center" valign="top" charoff="50"><bold>20.48 ± 5.99</bold></td><td align="center" valign="top" charoff="50"><bold>14.16 ± 0.68</bold></td><td align="center" valign="top" charoff="50"><bold>8.53 ± 1.7</bold></td></tr><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">Dicaffeoylquinic acid III</td><td align="center" valign="top" charoff="50">14.51 ± 6.25</td><td align="center" valign="top" charoff="50">67.66 ± 44.24</td><td align="center" valign="top" charoff="50"><bold>75.64 ± 25.16</bold></td><td align="center" valign="top" charoff="50"><bold>47.85 ± 5.52</bold></td><td align="center" valign="top" charoff="50"><bold>53.8 ± 7.82</bold></td></tr><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">Vanillic acid hexoside</td><td align="center" valign="top" charoff="50">1.04 ± 0.65</td><td align="center" valign="top" charoff="50">3.04 ± 1.98</td><td align="center" valign="top" charoff="50"><bold>7.06 ± 2.9</bold></td><td align="center" valign="top" charoff="50"><bold>6.5 ± 2.17</bold></td><td align="center" valign="top" charoff="50"><bold>3.33 ± 1.74</bold></td></tr><tr><td align="left" valign="top" charoff="50">Up</td><td align="left" valign="top" charoff="50">Protocatechuic acid-hexoside</td><td align="center" valign="top" charoff="50">1.27 ± 0.63</td><td align="center" valign="top" charoff="50">7.03 ± 6.4</td><td align="center" valign="top" charoff="50"><bold>8.32 ± 3.82</bold></td><td align="center" valign="top" charoff="50"><bold>3.12 ± 1.33</bold></td><td align="center" valign="top" charoff="50">1.06 ± 0.34</td></tr><tr><td align="left" valign="top" charoff="50">Down</td><td align="left" valign="top" charoff="50">L-Tyrosine</td><td align="center" valign="top" charoff="50">22.02 ± 6.53</td><td align="center" valign="top" charoff="50"><bold>7.6 ± 2.49</bold></td><td align="center" valign="top" charoff="50">12.62 ± 4.92</td><td align="center" valign="top" charoff="50"><bold>9.4 ± 0.96</bold></td><td align="center" valign="top" charoff="50">13.14 ± 5.96</td></tr><tr><td align="left" valign="top" charoff="50">Down</td><td align="left" valign="top" charoff="50">L-Phenylalanine</td><td align="center" valign="top" charoff="50">53.17 ± 4.01</td><td align="center" valign="top" charoff="50"><bold>23.81 ± 11.53</bold></td><td align="center" valign="top" charoff="50"><bold>19.8 ± 7.53</bold></td><td align="center" valign="top" charoff="50"><bold>23.64 ± 3.58</bold></td><td align="center" valign="top" charoff="50"><bold>31.01 ± 11.42</bold></td></tr><tr><td align="left" valign="top" charoff="50">Down</td><td align="left" valign="top" charoff="50">L-Tryptophan</td><td align="center" valign="top" charoff="50">19.18 ± 2.7</td><td align="center" valign="top" charoff="50"><bold>9.18 ± 5.51</bold></td><td align="center" valign="top" charoff="50">17.91 ± 4.15</td><td align="center" valign="top" charoff="50"><bold>14.18 ± 1.37</bold></td><td align="center" valign="top" charoff="50"><bold>10.97 ± 4.46</bold></td></tr><tr><td align="left" valign="top" charoff="50">No change</td><td align="left" valign="top" charoff="50">Coumaric acid</td><td align="center" valign="top" charoff="50">3.32 ± 2.2</td><td align="center" valign="top" charoff="50">6.35 ± 3.27</td><td align="center" valign="top" charoff="50"><bold>9.45 ± 3.82</bold></td><td align="center" valign="top" charoff="50">4.6 ± 0.57</td><td align="center" valign="top" charoff="50">6.92 ± 2.65</td></tr><tr><td align="left" valign="top" charoff="50">No change</td><td align="left" valign="top" charoff="50">Rutin</td><td align="center" valign="top" charoff="50">34.76 ± 11.31</td><td align="center" valign="top" charoff="50">47.71 ± 14.64</td><td align="center" valign="top" charoff="50">55.34 ± 13.6</td><td align="center" valign="top" charoff="50">35.08 ± 9.25</td><td align="center" valign="top" charoff="50"><bold>60.4 ± 5.31</bold></td></tr><tr><td align="left" valign="top" charoff="50">No change</td><td align="left" valign="top" charoff="50">Kaempferol rutinoside</td><td align="center" valign="top" charoff="50">0.66 ± 0.28</td><td align="center" valign="top" charoff="50">0.56 ± 0.12</td><td align="center" valign="top" charoff="50">0.76 ± 0.36</td><td align="center" valign="top" charoff="50">0.7 ± 0.29</td><td align="center" valign="top" charoff="50">0.47 ± 0.1</td></tr><tr><td align="left" valign="top" charoff="50">No change</td><td align="left" valign="top" charoff="50">Eriodictyol chalcone</td><td align="center" valign="top" charoff="50">2.13 ± 0.93</td><td align="center" valign="top" charoff="50">1.71 ± 0.79</td><td align="center" valign="top" charoff="50">2.24 ± 0.54</td><td align="center" valign="top" charoff="50">1.4 ± 0.13</td><td align="center" valign="top" charoff="50">1.94 ± 0.29</td></tr><tr><td align="left" valign="top" charoff="50">No change</td><td align="left" valign="top" charoff="50">Naringenin chalcone</td><td align="center" valign="top" charoff="50">111.97 ± 59.58</td><td align="center" valign="top" charoff="50">96.91 ± 26.25</td><td align="center" valign="top" charoff="50">84.6 ± 26.53</td><td align="center" valign="top" charoff="50">55.85 ± 8.89</td><td align="center" valign="top" charoff="50">131.43 ± 30.63</td></tr></tbody></table><table-wrap-foot><fn id="t1-fn1"><p>DW, dry weight. Data presenting mean value ± SD of four biological replicates. Values in bold denote significant differences as determined by <italic>t</italic>-test analysis (<italic>P</italic> < 0.05). The compounds contents differ significantly from WT in at least two out of four transgenic lines as indicated in the status row: Up, upregulated; Down, downregulated; while differ significantly only in one transgenic line or no significant alterations compared to WT were labeled ‘No change’ (some representative compounds were listed).</p></fn></table-wrap-foot></table-wrap><table-wrap position="float" id="t2"><label>Table 2</label><caption><title>Gene expression changes for selected genes which were significantly induced in <italic>CsMYBF1</italic>-overexpressing tomato fruits.</title></caption><table frame="hsides" rules="groups" border="1"><colgroup><col align="left"></col><col align="center"></col><col align="center"></col><col align="center"></col></colgroup><thead valign="bottom"><tr><th align="left" valign="top" charoff="50">Gene_id</th><th align="center" valign="top" charoff="50">Log<sub>2</sub> Fold Change</th><th align="center" valign="top" charoff="50"><italic>P</italic> adj.</th><th align="center" valign="top" charoff="50">Description</th></tr></thead><tbody valign="top"><tr><td colspan="4" align="left" valign="top" charoff="50">Glycolysis</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc03g093520.2</td><td align="center" valign="top" charoff="50">1.67</td><td align="center" valign="top" charoff="50">0.0367</td><td align="center" valign="top" charoff="50">6-phosphofructokinase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc12g095880.1</td><td align="center" valign="top" charoff="50">1.88</td><td align="center" valign="top" charoff="50">0.0048</td><td align="center" valign="top" charoff="50">6-phosphofructokinase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc04g072800.2</td><td align="center" valign="top" charoff="50">2.46</td><td align="center" valign="top" charoff="50">0.0012</td><td align="center" valign="top" charoff="50">Phosphoglycerate mutase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc10g085550.1</td><td align="center" valign="top" charoff="50">2.17</td><td align="center" valign="top" charoff="50">0.042</td><td align="center" valign="top" charoff="50">Enolase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc03g114500.2</td><td align="center" valign="top" charoff="50">3.05</td><td align="center" valign="top" charoff="50">1.96E-05</td><td align="center" valign="top" charoff="50">Enolase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc06g076650.2</td><td align="center" valign="top" charoff="50">1.63</td><td align="center" valign="top" charoff="50">0.0212</td><td align="center" valign="top" charoff="50">Enolase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc09g008840.2</td><td align="center" valign="top" charoff="50">1.33</td><td align="center" valign="top" charoff="50">0.0466</td><td align="center" valign="top" charoff="50">Pyruvate kinase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc06g051930.2</td><td align="center" valign="top" charoff="50">1.4</td><td align="center" valign="top" charoff="50">0.0466</td><td align="center" valign="top" charoff="50">Pyruvate kinase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc11g045370.1</td><td align="center" valign="top" charoff="50">1.91</td><td align="center" valign="top" charoff="50">0.0443</td><td align="center" valign="top" charoff="50">Lactate dehydrogenase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc04g064710.2</td><td align="center" valign="top" charoff="50">6.75</td><td align="center" valign="top" charoff="50">0.0486</td><td align="center" valign="top" charoff="50">Alcohol dehydrogenase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc06g072160.2</td><td align="center" valign="top" charoff="50">3.62</td><td align="center" valign="top" charoff="50">0.0001</td><td align="center" valign="top" charoff="50">Alcohol dehydrogenase</td></tr><tr><td colspan="4" align="left" valign="top" charoff="50">Pentose phosphate pathway</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc05g015950.2</td><td align="center" valign="top" charoff="50">1.78</td><td align="center" valign="top" charoff="50">0.0101</td><td align="center" valign="top" charoff="50">Glucose-6-phosphate 1-dehydrogenase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc02g093830.2</td><td align="center" valign="top" charoff="50">2.29</td><td align="center" valign="top" charoff="50">0.0002</td><td align="center" valign="top" charoff="50">Glucose-6-phosphate 1-dehydrogenase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc12g014380.1</td><td align="center" valign="top" charoff="50">1.89</td><td align="center" valign="top" charoff="50">0.0026</td><td align="center" valign="top" charoff="50">Glucose-6-phosphate isomerase</td></tr><tr><td colspan="4" align="left" valign="top" charoff="50">Sucrose biosynthesis</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc07g042550.2</td><td align="center" valign="top" charoff="50">2.15</td><td align="center" valign="top" charoff="50">0.0005</td><td align="center" valign="top" charoff="50">Sucrose synthase 1</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc03g098290.2</td><td align="center" valign="top" charoff="50">2.74</td><td align="center" valign="top" charoff="50">0.0002</td><td align="center" valign="top" charoff="50">Sucrose synthase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc02g081300.2</td><td align="center" valign="top" charoff="50">2.25</td><td align="center" valign="top" charoff="50">0.0366</td><td align="center" valign="top" charoff="50">Sucrose synthase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc07g007790.2</td><td align="center" valign="top" charoff="50">1.48</td><td align="center" valign="top" charoff="50">0.0178</td><td align="center" valign="top" charoff="50">Sucrose phosphate synthase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc10g081660.1</td><td align="center" valign="top" charoff="50">1.67</td><td align="center" valign="top" charoff="50">0.0159</td><td align="center" valign="top" charoff="50">Sucrose phosphatase</td></tr><tr><td colspan="4" align="left" valign="top" charoff="50">Shikimate pathway</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc02g083590.2</td><td align="center" valign="top" charoff="50">1.43</td><td align="center" valign="top" charoff="50">0.0353</td><td align="center" valign="top" charoff="50">Dehydroquinate synthase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc01g067750.2</td><td align="center" valign="top" charoff="50">2.17</td><td align="center" valign="top" charoff="50">0.001</td><td align="center" valign="top" charoff="50">3-dehydroquinate dehydratase/shikimate dehydrogenase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc04g051860.2</td><td align="center" valign="top" charoff="50">2.4</td><td align="center" valign="top" charoff="50">0.0004</td><td align="center" valign="top" charoff="50">Shikimate kinase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc05g050980.2</td><td align="center" valign="top" charoff="50">2.87</td><td align="center" valign="top" charoff="50">0.0012</td><td align="center" valign="top" charoff="50">EPSP synthase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc04g049350.2</td><td align="center" valign="top" charoff="50">2.68</td><td align="center" valign="top" charoff="50">4.43E-05</td><td align="center" valign="top" charoff="50">Chorismate synthase 1</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc02g088460.2</td><td align="center" valign="top" charoff="50">1.9</td><td align="center" valign="top" charoff="50">0.0115</td><td align="center" valign="top" charoff="50">Chorismate mutase</td></tr><tr><td colspan="4" align="left" valign="top" charoff="50">Phenylpropanoid pathway</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc00g282510.1</td><td align="center" valign="top" charoff="50">3.31</td><td align="center" valign="top" charoff="50">0.0019</td><td align="center" valign="top" charoff="50">Phe ammonia-lyase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc09g007900.2</td><td align="center" valign="top" charoff="50">2.65</td><td align="center" valign="top" charoff="50">0.0011</td><td align="center" valign="top" charoff="50">Phe ammonia-lyase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc09g007910.2</td><td align="center" valign="top" charoff="50">3.5</td><td align="center" valign="top" charoff="50">2.08E-07</td><td align="center" valign="top" charoff="50">Phe ammonia-lyase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc09g007920.2</td><td align="center" valign="top" charoff="50">3.82</td><td align="center" valign="top" charoff="50">4.44E-08</td><td align="center" valign="top" charoff="50">Phe ammonia-lyase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc10g011930.1</td><td align="center" valign="top" charoff="50">4.38</td><td align="center" valign="top" charoff="50">0.0048</td><td align="center" valign="top" charoff="50">Phe ammonia-lyase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc10g086180.1</td><td align="center" valign="top" charoff="50">3.82</td><td align="center" valign="top" charoff="50">1.45E-05</td><td align="center" valign="top" charoff="50">Phe ammonia-lyase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc09g007930.2</td><td align="center" valign="top" charoff="50">1.95</td><td align="center" valign="top" charoff="50">0.0172</td><td align="center" valign="top" charoff="50">Phe ammonia-lyase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc05g047530.2</td><td align="center" valign="top" charoff="50">3.81</td><td align="center" valign="top" charoff="50">0.0006</td><td align="center" valign="top" charoff="50">Cinnamate 4-hydroxylase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc03g117870.2</td><td align="center" valign="top" charoff="50">2.01</td><td align="center" valign="top" charoff="50">0.0027</td><td align="center" valign="top" charoff="50">4-Coumarate-CoA ligase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc03g097030.2</td><td align="center" valign="top" charoff="50">2.58</td><td align="center" valign="top" charoff="50">0.0037</td><td align="center" valign="top" charoff="50">4-Coumarate-CoA ligase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc10g078240.1</td><td align="center" valign="top" charoff="50">2.17</td><td align="center" valign="top" charoff="50">0.0039</td><td align="center" valign="top" charoff="50">Coumarate 3-hydrolase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc08g076780.1</td><td align="center" valign="top" charoff="50">4.52</td><td align="center" valign="top" charoff="50">0.001</td><td align="center" valign="top" charoff="50">Cinnamoyl-CoA reductase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc05g052240.2</td><td align="center" valign="top" charoff="50">1.6</td><td align="center" valign="top" charoff="50">0.0201</td><td align="center" valign="top" charoff="50">Chalcone isomerase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc02g083860.2</td><td align="center" valign="top" charoff="50">1.45</td><td align="center" valign="top" charoff="50">0.0295</td><td align="center" valign="top" charoff="50">Flavanone 3-hydroxylase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc03g115220.2</td><td align="center" valign="top" charoff="50">2.38</td><td align="center" valign="top" charoff="50">0.0031</td><td align="center" valign="top" charoff="50">Flavonoid 3′-hydroxylase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc11g013110.1</td><td align="center" valign="top" charoff="50">1.95</td><td align="center" valign="top" charoff="50">0.0026</td><td align="center" valign="top" charoff="50">Flavonol synthase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc02g089770.2</td><td align="center" valign="top" charoff="50">1.91</td><td align="center" valign="top" charoff="50">0.0036</td><td align="center" valign="top" charoff="50">Dihydroflavonol 4-reductase</td></tr><tr><td align="left" valign="top" charoff="50"> Solyc08g006770.2</td><td align="center" valign="top" charoff="50">2.53</td><td align="center" valign="top" charoff="50">0.0006</td><td align="center" valign="top" charoff="50">Anthocyanidin synthase</td></tr></tbody></table></table-wrap></floats-group></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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