Physiological and Proteomic Investigations to Study the Response of Tomato Graft Unions under Temperature Stress
Identifieur interne : 000392 ( Pmc/Curation ); précédent : 000391; suivant : 000393Physiological and Proteomic Investigations to Study the Response of Tomato Graft Unions under Temperature Stress
Auteurs : Sowbiya Muneer [Corée du Sud] ; Chung Ho Ko [Corée du Sud] ; Hao Wei [Corée du Sud] ; Yuze Chen [Corée du Sud] ; Byoung Ryong Jeong [Corée du Sud]Source :
- PLoS ONE [ 1932-6203 ] ; 2016.
Abstract
Grafting is an established practice for asexual propagation in horticultural and agricultural crops. The study on graft unions has become of interest for horticulturists using proteomic and genomic techniques to observe transfer of genetic material and signal transduction pathways from root to shoot and shoot to root. Another reason to study the graft unions was potentially to observe resistance against abiotic stresses. Using physiological and proteomic analyses, we investigated graft unions (rootstock and scions) of tomato genotypes exposed to standard-normal (23/23 and 25/18°C day/night) and high-low temperatures (30/15°C day/night).
Graft unions had varied responses to the diverse temperatures. High-low temperature, but not standard-normal temperature, induced the production of reactive oxygen species (ROS) in the form of H2O2 and O2-1 in rootstock and scions. However, the expression of many cell protection molecules was also induced, including antioxidant enzymes and their immunoblots, which also show an increase in their activities such as superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX). The graft interfaces thus actively defend against stress by modifying their physiological and proteomic responses to establish a new cellular homeostasis. As a result, many proteins for cellular defense were regulated in graft unions under diverse temperature, in addition to the regulation of photosynthetic proteins, ion binding/transport proteins, and protein synthesis. Moreover, biomass, hardness, and vascular transport activity were evaluated to investigate the basic connectivity between rootstock and scions.
Our study provides physiological evidence of the grafted plants’ response to diverse temperature. Most notably, our study provides novel insight into the mechanisms used to adapt the diverse temperature in graft unions (rootstock/scion).
Url:
DOI: 10.1371/journal.pone.0157439
PubMed: 27310261
PubMed Central: 4911148
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Korea</addr-line></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701</wicri:regionArea></affiliation></author><author><name sortKey="Wei, Hao" sort="Wei, Hao" uniqKey="Wei H" first="Hao" last="Wei">Hao Wei</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701, Korea</addr-line></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Yuze" sort="Chen, Yuze" uniqKey="Chen Y" first="Yuze" last="Chen">Yuze Chen</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701, Korea</addr-line></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701</wicri:regionArea></affiliation></author><author><name sortKey="Jeong, Byoung Ryong" sort="Jeong, Byoung Ryong" uniqKey="Jeong B" first="Byoung Ryong" last="Jeong">Byoung Ryong Jeong</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701, Korea</addr-line></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 660–701, Korea</addr-line></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 660–701</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>Research Institute of Life Science, Gyeongsang National University, Jinju, 660–701, Korea</addr-line></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Institute of Life Science, Gyeongsang National University, Jinju, 660–701</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27310261</idno><idno type="pmc">4911148</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911148</idno><idno type="RBID">PMC:4911148</idno><idno type="doi">10.1371/journal.pone.0157439</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000393</idno><idno type="wicri:Area/Pmc/Curation">000392</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Physiological and Proteomic Investigations to Study the Response of Tomato Graft Unions under Temperature Stress</title><author><name sortKey="Muneer, Sowbiya" sort="Muneer, Sowbiya" uniqKey="Muneer S" first="Sowbiya" last="Muneer">Sowbiya Muneer</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701, Korea</addr-line></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701</wicri:regionArea></affiliation></author><author><name sortKey="Ko, Chung Ho" sort="Ko, Chung Ho" uniqKey="Ko C" first="Chung Ho" last="Ko">Chung Ho Ko</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701, Korea</addr-line></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701</wicri:regionArea></affiliation></author><author><name sortKey="Wei, Hao" sort="Wei, Hao" uniqKey="Wei H" first="Hao" last="Wei">Hao Wei</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701, Korea</addr-line></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Yuze" sort="Chen, Yuze" uniqKey="Chen Y" first="Yuze" last="Chen">Yuze Chen</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701, Korea</addr-line></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701</wicri:regionArea></affiliation></author><author><name sortKey="Jeong, Byoung Ryong" sort="Jeong, Byoung Ryong" uniqKey="Jeong B" first="Byoung Ryong" last="Jeong">Byoung Ryong Jeong</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701, Korea</addr-line></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 660–701, Korea</addr-line></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 660–701</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>Research Institute of Life Science, Gyeongsang National University, Jinju, 660–701, Korea</addr-line></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Institute of Life Science, Gyeongsang National University, Jinju, 660–701</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="sec001"><title>Background</title><p>Grafting is an established practice for asexual propagation in horticultural and agricultural crops. The study on graft unions has become of interest for horticulturists using proteomic and genomic techniques to observe transfer of genetic material and signal transduction pathways from root to shoot and shoot to root. Another reason to study the graft unions was potentially to observe resistance against abiotic stresses. Using physiological and proteomic analyses, we investigated graft unions (rootstock and scions) of tomato genotypes exposed to standard-normal (23/23 and 25/18°C day/night) and high-low temperatures (30/15°C day/night).</p></sec><sec id="sec002"><title>Results</title><p>Graft unions had varied responses to the diverse temperatures. High-low temperature, but not standard-normal temperature, induced the production of reactive oxygen species (ROS) in the form of H<sub>2</sub>O<sub>2</sub> and O<sub>2</sub><sup>-1</sup> in rootstock and scions. However, the expression of many cell protection molecules was also induced, including antioxidant enzymes and their immunoblots, which also show an increase in their activities such as superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX). The graft interfaces thus actively defend against stress by modifying their physiological and proteomic responses to establish a new cellular homeostasis. As a result, many proteins for cellular defense were regulated in graft unions under diverse temperature, in addition to the regulation of photosynthetic proteins, ion binding/transport proteins, and protein synthesis. Moreover, biomass, hardness, and vascular transport activity were evaluated to investigate the basic connectivity between rootstock and scions.</p></sec><sec id="sec003"><title>Conclusions</title><p>Our study provides physiological evidence of the grafted plants’ response to diverse temperature. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27310261</article-id><article-id pub-id-type="pmc">4911148</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0157439</article-id><article-id pub-id-type="publisher-id">PONE-D-15-49254</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Plant Biochemistry</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Biochemistry</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Agriculture</subject><subj-group><subject>Crop Science</subject><subj-group><subject>Crops</subject><subj-group><subject>Fruits</subject><subj-group><subject>Tomatoes</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Plants</subject><subj-group><subject>Fruits</subject><subj-group><subject>Tomatoes</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and life sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Proteins</subject><subj-group><subject>DNA-binding proteins</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Physiology</subject><subj-group><subject>Plant Defenses</subject><subj-group><subject>Plant Resistance to Abiotic Stress</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Pathology</subject><subj-group><subject>Plant Resistance to Abiotic Stress</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Ecology</subject><subj-group><subject>Plant Ecology</subject><subj-group><subject>Plant-Environment Interactions</subject><subj-group><subject>Plant Resistance to Abiotic Stress</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Ecology and Environmental Sciences</subject><subj-group><subject>Ecology</subject><subj-group><subject>Plant Ecology</subject><subj-group><subject>Plant-Environment Interactions</subject><subj-group><subject>Plant Resistance to Abiotic Stress</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Ecology</subject><subj-group><subject>Plant-Environment Interactions</subject><subj-group><subject>Plant Resistance to Abiotic Stress</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Plant Biochemistry</subject><subj-group><subject>Photosynthesis</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Biochemistry</subject><subj-group><subject>Photosynthesis</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Physiology</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Proteomics</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Proteins</subject><subj-group><subject>Chaperone Proteins</subject></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Physiological and Proteomic Investigations to Study the Response of Tomato Graft Unions under Temperature Stress</article-title><alt-title alt-title-type="running-head">New Insights from Proteome of Tomato Graft Unions Exposed to Temperature Stress</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Muneer</surname><given-names>Sowbiya</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ko</surname><given-names>Chung Ho</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="author-notes" rid="currentaff001"><sup>¤a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Wei</surname><given-names>Hao</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Yuze</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="author-notes" rid="currentaff002"><sup>¤b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Jeong</surname><given-names>Byoung Ryong</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="aff" rid="aff002"><sup>2</sup></xref><xref ref-type="aff" rid="aff003"><sup>3</sup></xref><xref ref-type="corresp" rid="cor001">*</xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, 660–701, Korea</addr-line></aff><aff id="aff002"><label>2</label><addr-line>Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 660–701, Korea</addr-line></aff><aff id="aff003"><label>3</label><addr-line>Research Institute of Life Science, Gyeongsang National University, Jinju, 660–701, Korea</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Dai</surname><given-names>Shaojun</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Northeast Forestry University, CHINA</addr-line></aff><author-notes><fn fn-type="conflict" id="coi001"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con" id="contrib001"><p>Conceived and designed the experiments: SM BRJ. Performed the experiments: SM CHK. Analyzed the data: SM. Contributed reagents/materials/analysis tools: BRJ. Wrote the paper: SM BRJ. Helped in physiological data collection: HW YC.</p></fn><fn fn-type="current-aff" id="currentaff001"><label>¤a</label><p>Current address: Korea National Arboretum, Useful Plant Resource Centre, 21–4, Dudam-gil, Yongmun-myeon, yangpyeong-gun, Gyeonggi-do, 12519, Korea</p></fn><fn fn-type="current-aff" id="currentaff002"><label>¤b</label><p>Current address: Solar Energy Base, Pudong Twon, Jimo, Qingdao City, Shandong Province, 266234, China</p></fn><corresp id="cor001">* E-mail: <email>brjeong@gnu.ac.kr</email></corresp></author-notes><pub-date pub-type="epub"><day>16</day><month>6</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>11</volume><issue>6</issue><elocation-id>e0157439</elocation-id><history><date date-type="received"><day>11</day><month>11</month><year>2015</year></date><date date-type="accepted"><day>31</day><month>5</month><year>2016</year></date></history><permissions><copyright-statement>© 2016 Muneer et al</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>Muneer et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="pone.0157439.pdf"></self-uri><abstract><sec id="sec001"><title>Background</title><p>Grafting is an established practice for asexual propagation in horticultural and agricultural crops. The study on graft unions has become of interest for horticulturists using proteomic and genomic techniques to observe transfer of genetic material and signal transduction pathways from root to shoot and shoot to root. Another reason to study the graft unions was potentially to observe resistance against abiotic stresses. Using physiological and proteomic analyses, we investigated graft unions (rootstock and scions) of tomato genotypes exposed to standard-normal (23/23 and 25/18°C day/night) and high-low temperatures (30/15°C day/night).</p></sec><sec id="sec002"><title>Results</title><p>Graft unions had varied responses to the diverse temperatures. High-low temperature, but not standard-normal temperature, induced the production of reactive oxygen species (ROS) in the form of H<sub>2</sub>O<sub>2</sub> and O<sub>2</sub><sup>-1</sup> in rootstock and scions. However, the expression of many cell protection molecules was also induced, including antioxidant enzymes and their immunoblots, which also show an increase in their activities such as superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX). The graft interfaces thus actively defend against stress by modifying their physiological and proteomic responses to establish a new cellular homeostasis. As a result, many proteins for cellular defense were regulated in graft unions under diverse temperature, in addition to the regulation of photosynthetic proteins, ion binding/transport proteins, and protein synthesis. Moreover, biomass, hardness, and vascular transport activity were evaluated to investigate the basic connectivity between rootstock and scions.</p></sec><sec id="sec003"><title>Conclusions</title><p>Our study provides physiological evidence of the grafted plants’ response to diverse temperature. Most notably, our study provides novel insight into the mechanisms used to adapt the diverse temperature in graft unions (rootstock/scion).</p></sec></abstract><funding-group><award-group id="award001"><funding-source><institution-wrap><institution-id institution-id-type="funder-id">http://dx.doi.org/10.13039/501100003668</institution-id><institution>Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries</institution></institution-wrap></funding-source><award-id>Project No. 312034-04</award-id><principal-award-recipient><name><surname>Jeong</surname><given-names>Byoung Ryong</given-names></name></principal-award-recipient></award-group><funding-statement>This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture Forestry and Fisheries (Project No. 312034-04). SM, CHK, HW and YC were supported by a scholarship from BK21 program, Ministry of Education, Korea.</funding-statement></funding-group><counts><fig-count count="9"></fig-count><table-count count="2"></table-count><page-count count="23"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>All data is presented in the manuscript and its Supporting Information files.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>All data is presented in the manuscript and its Supporting Information files.</p></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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