Molecular Analysis of Colletotrichum Species in the Carposphere and Phyllosphere of Olive
Identifieur interne : 000228 ( Pmc/Curation ); précédent : 000227; suivant : 000229Molecular Analysis of Colletotrichum Species in the Carposphere and Phyllosphere of Olive
Auteurs : Saveria Mosca [Italie] ; Maria G. Li Destri Nicosia [Italie] ; Santa O. Cacciola [Italie] ; Leonardo Schena [Italie]Source :
- PLoS ONE [ 1932-6203 ] ; 2014.
Abstract
A metagenomic approach based on the use of genus specific primers was developed and utilized to characterize
Url:
DOI: 10.1371/journal.pone.0114031
PubMed: 25501572
PubMed Central: 4263604
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular Analysis of <italic>Colletotrichum</italic> Species in the Carposphere and Phyllosphere of Olive</title><author><name sortKey="Mosca, Saveria" sort="Mosca, Saveria" uniqKey="Mosca S" first="Saveria" last="Mosca">Saveria Mosca</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Dipartimento di Agraria, Università Mediterranea, Reggio Calabria, Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Agraria, Università Mediterranea, Reggio Calabria</wicri:regionArea></affiliation></author><author><name sortKey="Li Destri Nicosia, Maria G" sort="Li Destri Nicosia, Maria G" uniqKey="Li Destri Nicosia M" first="Maria G." last="Li Destri Nicosia">Maria G. Li Destri Nicosia</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Dipartimento di Agraria, Università Mediterranea, Reggio Calabria, Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Agraria, Università Mediterranea, Reggio Calabria</wicri:regionArea></affiliation></author><author><name sortKey="Cacciola, Santa O" sort="Cacciola, Santa O" uniqKey="Cacciola S" first="Santa O." last="Cacciola">Santa O. Cacciola</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Dipartimento di Gestione dei Sistemi Agroalimentari e Ambientali, Università degli Studi, Catania, Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Gestione dei Sistemi Agroalimentari e Ambientali, Università degli Studi, Catania</wicri:regionArea></affiliation></author><author><name sortKey="Schena, Leonardo" sort="Schena, Leonardo" uniqKey="Schena L" first="Leonardo" last="Schena">Leonardo Schena</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Dipartimento di Agraria, Università Mediterranea, Reggio Calabria, Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Agraria, Università Mediterranea, Reggio Calabria</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25501572</idno><idno type="pmc">4263604</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263604</idno><idno type="RBID">PMC:4263604</idno><idno type="doi">10.1371/journal.pone.0114031</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000228</idno><idno type="wicri:Area/Pmc/Curation">000228</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Molecular Analysis of <italic>Colletotrichum</italic> Species in the Carposphere and Phyllosphere of Olive</title><author><name sortKey="Mosca, Saveria" sort="Mosca, Saveria" uniqKey="Mosca S" first="Saveria" last="Mosca">Saveria Mosca</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Dipartimento di Agraria, Università Mediterranea, Reggio Calabria, Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Agraria, Università Mediterranea, Reggio Calabria</wicri:regionArea></affiliation></author><author><name sortKey="Li Destri Nicosia, Maria G" sort="Li Destri Nicosia, Maria G" uniqKey="Li Destri Nicosia M" first="Maria G." last="Li Destri Nicosia">Maria G. Li Destri Nicosia</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Dipartimento di Agraria, Università Mediterranea, Reggio Calabria, Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Agraria, Università Mediterranea, Reggio Calabria</wicri:regionArea></affiliation></author><author><name sortKey="Cacciola, Santa O" sort="Cacciola, Santa O" uniqKey="Cacciola S" first="Santa O." last="Cacciola">Santa O. Cacciola</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Dipartimento di Gestione dei Sistemi Agroalimentari e Ambientali, Università degli Studi, Catania, Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Gestione dei Sistemi Agroalimentari e Ambientali, Università degli Studi, Catania</wicri:regionArea></affiliation></author><author><name sortKey="Schena, Leonardo" sort="Schena, Leonardo" uniqKey="Schena L" first="Leonardo" last="Schena">Leonardo Schena</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Dipartimento di Agraria, Università Mediterranea, Reggio Calabria, Italy</addr-line></nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Agraria, Università Mediterranea, Reggio Calabria</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A metagenomic approach based on the use of genus specific primers was developed and utilized to characterize <italic>Colletotrichum</italic> species associated with the olive phyllosphere and carposphere. Selected markers enabled the specific amplification of almost the entire ITS1-5.8S-ITS2 region of the rDNA and its use as barcode gene. The analysis of different olive samples (green and senescent leaves, floral residues, symptomatic and asymptomatic fruits, and litter leaves and mummies) in three different phenological phases (June, October and December) enabled the detection of 12 genotypes associated with 4 phylotypes identified as <italic>C. godetiae, C. acutatum s.s., C. gloeosporioides s.s</italic>. and <italic>C. kahawae</italic>. Another three genotypes were not identified at the level of species but were associated with the species complexes of <italic>C. acutatum</italic>, <italic>C. gloeosporioides</italic> and <italic>C. boninense sensu lato</italic>. <italic>Colletotrichum godetiae</italic> and <italic>C. acutatum s.s.</italic> were by far the most abundant while <italic>C. gloeosporioides s.s.</italic> was detected in a limited number of samples whereas ther phylotypes were rarely found. The high incidence of <italic>C. acutatum s.s.</italic> represents a novelty for Italy and more generally for the Mediterranean basin since it had been previously reported only in Portugal. As regards to the phenological phase, <italic>Colletotrichum</italic> species were found in a few samples in June and were diffused on all assessed samples in December. According to data new infections on olive tissues mainly occur in the late fall. Furthermore, <italic>Colletotrichum</italic> species seem to have a saprophytic behavior on floral olive residues. The method developed in the present study proved to be valuable and its future application may contribute to the study of cycle and aetiology of diseases caused by <italic>Colletotrichum</italic> species in many different pathosystems.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Cannon, P" uniqKey="Cannon P">P Cannon</name></author><author><name sortKey="Damm, U" uniqKey="Damm U">U Damm</name></author><author><name sortKey="Johnston, P" uniqKey="Johnston P">P Johnston</name></author><author><name sortKey="Weir, B" uniqKey="Weir B">B Weir</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cacciola, S" uniqKey="Cacciola S">S Cacciola</name></author><author><name sortKey="Faedda, R" uniqKey="Faedda R">R Faedda</name></author><author><name sortKey="Sinatra, F" uniqKey="Sinatra F">F Sinatra</name></author><author><name sortKey="Agosteo, G" uniqKey="Agosteo G">G Agosteo</name></author><author><name 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uniqKey="Sanzani S">SM Sanzani</name></author><author><name sortKey="Schena, L" uniqKey="Schena L">L Schena</name></author><author><name sortKey="De Cicco, V" uniqKey="De Cicco V">V De Cicco</name></author><author><name sortKey="Ippolito, A" uniqKey="Ippolito A">A Ippolito</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Almeida, M" uniqKey="Almeida M">M Almeida</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25501572</article-id><article-id pub-id-type="pmc">4263604</article-id><article-id pub-id-type="publisher-id">PONE-D-14-31143</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0114031</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology and Life Sciences</subject><subj-group><subject>Agriculture</subject></subj-group><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Pathology</subject><subj-group><subject>Plant Pathogens</subject><subj-group><subject>Plant Fungal Pathogens</subject></subj-group></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Molecular Analysis of <italic>Colletotrichum</italic> Species in the Carposphere and Phyllosphere of Olive</article-title><alt-title alt-title-type="running-head">Molecular Analysis of <italic>Colletotrichum</italic> Species</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Mosca</surname><given-names>Saveria</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Li Destri Nicosia</surname><given-names>Maria G.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Cacciola</surname><given-names>Santa O.</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Schena</surname><given-names>Leonardo</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Dipartimento di Agraria, Università Mediterranea, Reggio Calabria, Italy</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Dipartimento di Gestione dei Sistemi Agroalimentari e Ambientali, Università degli Studi, Catania, Italy</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Al-Ahmad</surname><given-names>Ali</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>University Hospital of the Albert-Ludwigs-University Freiburg, Germany</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>lschena@unirc.it</email></corresp><fn fn-type="conflict"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: LS. Performed the experiments: SM. Analyzed the data: SM LS. Contributed reagents/materials/analysis tools: SM MGLDN. Wrote the paper: LS SOC.</p></fn></author-notes><pub-date pub-type="collection"><year>2014</year></pub-date><pub-date pub-type="epub"><day>11</day><month>12</month><year>2014</year></pub-date><volume>9</volume><issue>12</issue><elocation-id>e114031</elocation-id><history><date date-type="received"><day>17</day><month>7</month><year>2014</year></date><date date-type="accepted"><day>3</day><month>11</month><year>2014</year></date></history><permissions><copyright-year>2014</copyright-year><copyright-holder>Mosca et al</copyright-holder><license><license-p>This is an open-access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><abstract><p>A metagenomic approach based on the use of genus specific primers was developed and utilized to characterize <italic>Colletotrichum</italic> species associated with the olive phyllosphere and carposphere. Selected markers enabled the specific amplification of almost the entire ITS1-5.8S-ITS2 region of the rDNA and its use as barcode gene. The analysis of different olive samples (green and senescent leaves, floral residues, symptomatic and asymptomatic fruits, and litter leaves and mummies) in three different phenological phases (June, October and December) enabled the detection of 12 genotypes associated with 4 phylotypes identified as <italic>C. godetiae, C. acutatum s.s., C. gloeosporioides s.s</italic>. and <italic>C. kahawae</italic>. Another three genotypes were not identified at the level of species but were associated with the species complexes of <italic>C. acutatum</italic>, <italic>C. gloeosporioides</italic> and <italic>C. boninense sensu lato</italic>. <italic>Colletotrichum godetiae</italic> and <italic>C. acutatum s.s.</italic> were by far the most abundant while <italic>C. gloeosporioides s.s.</italic> was detected in a limited number of samples whereas ther phylotypes were rarely found. The high incidence of <italic>C. acutatum s.s.</italic> represents a novelty for Italy and more generally for the Mediterranean basin since it had been previously reported only in Portugal. As regards to the phenological phase, <italic>Colletotrichum</italic> species were found in a few samples in June and were diffused on all assessed samples in December. According to data new infections on olive tissues mainly occur in the late fall. Furthermore, <italic>Colletotrichum</italic> species seem to have a saprophytic behavior on floral olive residues. The method developed in the present study proved to be valuable and its future application may contribute to the study of cycle and aetiology of diseases caused by <italic>Colletotrichum</italic> species in many different pathosystems.</p></abstract><funding-group><funding-statement>This work was funded by grant MIUR-FIRB 2010 (RBFR10PZ4N) from the Italian Ministry of Education, University and Research (MIUR). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="21"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>The authors confirm that all data underlying the findings are fully available without restriction. All relevant data are within the paper.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>The authors confirm that all data underlying the findings are fully available without restriction. All relevant data are within the paper.</p></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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