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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">5-demethyltangeretin inhibits human non-small cell lung cancer cell growth by inducing G2/M cell cycle arrest and apoptosis</title><author><name sortKey="Charoensinphon, Noppawat" sort="Charoensinphon, Noppawat" uniqKey="Charoensinphon N" first="Noppawat" last="Charoensinphon">Noppawat Charoensinphon</name><affiliation><nlm:aff id="A1">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff></affiliation></author><author><name sortKey="Qiu, Peiju" sort="Qiu, Peiju" uniqKey="Qiu P" first="Peiju" last="Qiu">Peiju Qiu</name><affiliation><nlm:aff id="A1">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A2">Marine Drug and Food Institute, Ocean University of China, Qingdao, Shandong, China</nlm:aff></affiliation></author><author><name sortKey="Dong, Ping" sort="Dong, Ping" uniqKey="Dong P" first="Ping" last="Dong">Ping Dong</name><affiliation><nlm:aff id="A1">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff></affiliation></author><author><name sortKey="Zheng, Jinkai" sort="Zheng, Jinkai" uniqKey="Zheng J" first="Jinkai" last="Zheng">Jinkai Zheng</name><affiliation><nlm:aff id="A1">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff></affiliation></author><author><name sortKey="Ngauv, Pearline" sort="Ngauv, Pearline" uniqKey="Ngauv P" first="Pearline" last="Ngauv">Pearline Ngauv</name><affiliation><nlm:aff id="A1">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff></affiliation></author><author><name sortKey="Cao, Yong" sort="Cao, Yong" uniqKey="Cao Y" first="Yong" last="Cao">Yong Cao</name><affiliation><nlm:aff id="A3">College of Food Science, South China Agricultural University, Guangzhou, Guangdong, China</nlm:aff></affiliation></author><author><name sortKey="Li, Shiming" sort="Li, Shiming" uniqKey="Li S" first="Shiming" last="Li">Shiming Li</name><affiliation><nlm:aff id="A4">Department of Food Science, Rutgers, the State University of New Jersey, New Brunswick, NJ, USA</nlm:aff></affiliation></author><author><name sortKey="Ho, Chi Tang" sort="Ho, Chi Tang" uniqKey="Ho C" first="Chi-Tang" last="Ho">Chi-Tang Ho</name><affiliation><nlm:aff id="A4">Department of Food Science, Rutgers, the State University of New Jersey, New Brunswick, NJ, USA</nlm:aff></affiliation></author><author><name sortKey="Xiao, Hang" sort="Xiao, Hang" uniqKey="Xiao H" first="Hang" last="Xiao">Hang Xiao</name><affiliation><nlm:aff id="A1">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23926120</idno><idno type="pmc">4648632</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648632</idno><idno type="RBID">PMC:4648632</idno><idno type="doi">10.1002/mnfr.201300136</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000D66</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">5-demethyltangeretin inhibits human non-small cell lung cancer cell growth by inducing G2/M cell cycle arrest and apoptosis</title><author><name sortKey="Charoensinphon, Noppawat" sort="Charoensinphon, Noppawat" uniqKey="Charoensinphon N" first="Noppawat" last="Charoensinphon">Noppawat Charoensinphon</name><affiliation><nlm:aff id="A1">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff></affiliation></author><author><name sortKey="Qiu, Peiju" sort="Qiu, Peiju" uniqKey="Qiu P" first="Peiju" last="Qiu">Peiju Qiu</name><affiliation><nlm:aff id="A1">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A2">Marine Drug and Food Institute, Ocean University of China, Qingdao, Shandong, China</nlm:aff></affiliation></author><author><name sortKey="Dong, Ping" sort="Dong, Ping" uniqKey="Dong P" first="Ping" last="Dong">Ping Dong</name><affiliation><nlm:aff id="A1">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff></affiliation></author><author><name sortKey="Zheng, Jinkai" sort="Zheng, Jinkai" uniqKey="Zheng J" first="Jinkai" last="Zheng">Jinkai Zheng</name><affiliation><nlm:aff id="A1">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff></affiliation></author><author><name sortKey="Ngauv, Pearline" sort="Ngauv, Pearline" uniqKey="Ngauv P" first="Pearline" last="Ngauv">Pearline Ngauv</name><affiliation><nlm:aff id="A1">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff></affiliation></author><author><name sortKey="Cao, Yong" sort="Cao, Yong" uniqKey="Cao Y" first="Yong" last="Cao">Yong Cao</name><affiliation><nlm:aff id="A3">College of Food Science, South China Agricultural University, Guangzhou, Guangdong, China</nlm:aff></affiliation></author><author><name sortKey="Li, Shiming" sort="Li, Shiming" uniqKey="Li S" first="Shiming" last="Li">Shiming Li</name><affiliation><nlm:aff id="A4">Department of Food Science, Rutgers, the State University of New Jersey, New Brunswick, NJ, USA</nlm:aff></affiliation></author><author><name sortKey="Ho, Chi Tang" sort="Ho, Chi Tang" uniqKey="Ho C" first="Chi-Tang" last="Ho">Chi-Tang Ho</name><affiliation><nlm:aff id="A4">Department of Food Science, Rutgers, the State University of New Jersey, New Brunswick, NJ, USA</nlm:aff></affiliation></author><author><name sortKey="Xiao, Hang" sort="Xiao, Hang" uniqKey="Xiao H" first="Hang" last="Xiao">Hang Xiao</name><affiliation><nlm:aff id="A1">Department of Food Science, University of Massachusetts, Amherst, MA, USA</nlm:aff></affiliation></author></analytic><series><title level="j">Molecular nutrition & food research</title><idno type="ISSN">1613-4125</idno><idno type="eISSN">1613-4133</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="S1"><title>Scope</title><p id="P1">Tangeretin and 5-demethyltangeretin (5DT) are two closely related polymethoxyflavones found in citrus fruits. We investigated growth inhibitory effects on three human non-small cell lung cancer (NSCLC) cells.</p></sec><sec id="S2"><title>Methods and results</title><p id="P2">Cell viability assay demonstrated that 5DT inhibited NSCLC cell growth in a time- and dose-dependent manner, and IC<sub>50</sub>s of 5DT were 79-fold, 57-fold and 56-fold lower than those of tangeretin in A549, H460, and H1299 cells, respectively. Flow cytometry analysis showed that 5DT induced extensive G2/M cell cycle arrest and apoptosis in NSCLC cells, while tangeretin at 10-fold higher concentrations did not. The apoptosis induced by 5DT was further confirmed by activation of caspase-3 and cleavage of PARP. Moreover, 5DT dose-dependently upregulated p53 and p21<sup>Cip1/Waf1</sup>, and downregulated Cdc-2 (Cdk-1) and cyclin B1. HPLC analysis revealed that the intracellular levels of 5DT in NSCLC cells were 2.7 - 4.9-fold higher than those of tangeretin after the cells were treated with 5DT or tangeretin at the same concentration.</p></sec><sec id="S3"><title>Conclusions</title><p id="P3">our results demonstrated that 5DT inhibited NSCLC cell growth by inducing G2/M cell cycle arrest and apoptosis. These effects were much stronger than those produced by tangeretin, which is partially due to the higher intracellular uptake of 5DT than tangeretin.</p></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
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<front><journal-meta><journal-id journal-id-type="nlm-journal-id">101231818</journal-id><journal-id journal-id-type="pubmed-jr-id">32150</journal-id><journal-id journal-id-type="nlm-ta">Mol Nutr Food Res</journal-id><journal-id journal-id-type="iso-abbrev">Mol Nutr Food Res</journal-id><journal-title-group><journal-title>Molecular nutrition & food research</journal-title></journal-title-group><issn pub-type="ppub">1613-4125</issn><issn pub-type="epub">1613-4133</issn></journal-meta><article-meta><article-id pub-id-type="pmid">23926120</article-id><article-id pub-id-type="pmc">4648632</article-id><article-id pub-id-type="doi">10.1002/mnfr.201300136</article-id><article-id pub-id-type="manuscript">NIHMS523157</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>5-demethyltangeretin inhibits human non-small cell lung cancer cell growth by inducing G2/M cell cycle arrest and apoptosis</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Charoensinphon</surname><given-names>Noppawat</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Qiu</surname><given-names>Peiju</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Dong</surname><given-names>Ping</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Zheng</surname><given-names>Jinkai</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Ngauv</surname><given-names>Pearline</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Cao</surname><given-names>Yong</given-names></name><xref ref-type="aff" rid="A3">3</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Shiming</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Ho</surname><given-names>Chi-Tang</given-names></name><xref ref-type="aff" rid="A4">4</xref></contrib><contrib contrib-type="author"><name><surname>Xiao</surname><given-names>Hang</given-names></name><xref ref-type="corresp" rid="CR1">*</xref><xref ref-type="aff" rid="A1">1</xref></contrib></contrib-group><aff id="A1"><label>1</label>Department of Food Science, University of Massachusetts, Amherst, MA, USA</aff><aff id="A2"><label>2</label>Marine Drug and Food Institute, Ocean University of China, Qingdao, Shandong, China</aff><aff id="A3"><label>3</label>College of Food Science, South China Agricultural University, Guangzhou, Guangdong, China</aff><aff id="A4"><label>4</label>Department of Food Science, Rutgers, the State University of New Jersey, New Brunswick, NJ, USA</aff><author-notes><corresp id="CR1"><label>*</label><bold>Corresponding Author:</bold> Hang Xiao, Department of Food Science, University of Massachusetts Amherst, 102 Holdsworth Way, Amherst, MA 01003, USA, Tel: (413) 545-2281; Fax: (413) 545-1262, <email>hangxiao@foodsci.umass.edu</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>13</day><month>11</month><year>2013</year></pub-date><pub-date pub-type="epub"><day>08</day><month>8</month><year>2013</year></pub-date><pub-date pub-type="ppub"><month>12</month><year>2013</year></pub-date><pub-date pub-type="pmc-release"><day>17</day><month>11</month><year>2015</year></pub-date><volume>57</volume><issue>12</issue><fpage>2103</fpage><lpage>2111</lpage><pmc-comment>elocation-id from pubmed: 10.1002/mnfr.201300136</pmc-comment>
<abstract><sec id="S1"><title>Scope</title><p id="P1">Tangeretin and 5-demethyltangeretin (5DT) are two closely related polymethoxyflavones found in citrus fruits. We investigated growth inhibitory effects on three human non-small cell lung cancer (NSCLC) cells.</p></sec><sec id="S2"><title>Methods and results</title><p id="P2">Cell viability assay demonstrated that 5DT inhibited NSCLC cell growth in a time- and dose-dependent manner, and IC<sub>50</sub>s of 5DT were 79-fold, 57-fold and 56-fold lower than those of tangeretin in A549, H460, and H1299 cells, respectively. Flow cytometry analysis showed that 5DT induced extensive G2/M cell cycle arrest and apoptosis in NSCLC cells, while tangeretin at 10-fold higher concentrations did not. The apoptosis induced by 5DT was further confirmed by activation of caspase-3 and cleavage of PARP. Moreover, 5DT dose-dependently upregulated p53 and p21<sup>Cip1/Waf1</sup>, and downregulated Cdc-2 (Cdk-1) and cyclin B1. HPLC analysis revealed that the intracellular levels of 5DT in NSCLC cells were 2.7 - 4.9-fold higher than those of tangeretin after the cells were treated with 5DT or tangeretin at the same concentration.</p></sec><sec id="S3"><title>Conclusions</title><p id="P3">our results demonstrated that 5DT inhibited NSCLC cell growth by inducing G2/M cell cycle arrest and apoptosis. These effects were much stronger than those produced by tangeretin, which is partially due to the higher intracellular uptake of 5DT than tangeretin.</p></sec></abstract><kwd-group><kwd>5-demethyltangeretin</kwd><kwd>5-hydroxy-6,7,8,4′-tetramethoxyflavone</kwd><kwd>apoptosis</kwd><kwd>lung cancer</kwd><kwd>tangeretin</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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