Seven healthy females and six males consumed daily 256 mg vitamin C, 229 mg hesperidin (main flavonoid occurring as glycoside), 6 mg carotenoids (mainly luteins and cryptoxanthins), and 0.16 mg folate by incorporation of daily 236 mL of not-from-concentrate orange juice (OJ) into their habitual diet. At the end of three weeks mean vitamin C, folate, carotenoid, and flavanone plasma concentrations increased significantly relative to baseline by 59% (p<0.001), 46% (p=0.018), and 22% (p<0.001), and 8 fold (p=0.045), respectively. Flavanones were excreted in urine 9 fold more at the end of the intervention (p=0.01) but returned to baseline two days after study completion. After the 3-week intervention plasma concentrations of vitamins A and E did not change. 8-Hydroxy deoxyguanosine (8OHdG) in white blood cells declined by 16% (p=0.38; n=11), and in individuals with high baseline concentrations by 29% (p=0.36; n=7), respectively. LDL-/HDL-cholesterol ratio decreased but cholesterol (HDL, LDL, total) and thiobarbituric acid reactive substance plasma concentrations did not change significantly. We conclude from this pilot study that OJ is an excellent food source to enhance circulating concentrations of valuable hydrophilic as well as lipophilic phytochemicals.